LOCUS       pnCas9-2-UGI-Csy4::Ptr 12179 bp ds-DNA     circular     10-DEC-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          447..467
                     /label="174_Cas9seq4"
                     /note="sequence: CAGATGGTTTTGCCAATCGCA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1100..1119
                     /label="50-Cas9-rv"
                     /note="sequence: GCTGAACGTGGAGGTTTGAG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(1686..1705)
                     /label="49-Cas9-fw"
                     /note="sequence: TCCGCCTGTCTGTACTTCTG"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(2011..2038)
                     /label="154_U_pMCRi_rev"
                     /note="sequence: AGCCAGCATCCGTTTACGACCGTTTTCT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2024..2040
                     /label="176_Cas9seq6"
                     /note="sequence: AAACGGATGCTGGCTAG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2030..2062
                     /label="151_U_fenix_for"
                     /note="sequence: ATGCTGGCTAGTGCCGGAGAATTACAAAAAGGA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2467..2487
                     /label="07> CRISPR_module_check"
                     /note="sequence: acgcattgatttgagtcagct"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          2467..2487
                     /label="07> CRISPR_module_check"
                     /note="sequence: ACGCATTGATTTGAGTCAGCT"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(2471..2498)
                     /label="cas9 USER Rv"
                     /note="sequence: AGTCACCTCCTAGCTGACTCAAATCAAT"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2473..2498)
                     /label="95_U-dCas9-rev"
                     /note="sequence: AGTCACCTCCTAGCTGACTCAAATCA"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(2473..2498)
                     /label="pnCas9PABEC-UGI-U-rev"
                     /note="sequence: AGTCACCTCCTAGCTGACTCAAATCA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          2491..2517
                     /label="UGI-Cas9-U-for"
                     /note="sequence: AGGTGACTCCGGCGGCTCCACCAACCT"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             2498..2509
                     /label="Translation 2498-2509"
     CDS             2510..2761
                     /label="streUGI"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             2510..2761
                     /label="Translation 2510-2761"
     primer          complement(2729..2759)
                     /label="UGI-Cas9-U-rev"
                     /note="sequence: ACAGCATCTTGATCTTGTTCTCGCCGTTGGA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2753..2781
                     /label="pnCas9PABEC-UGI-U-for"
                     /note="sequence: ATGCTGTGAACTTCGGAATAGGAACTTCA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2762..2781
                     /label="pnCas9PABEC-UGI-U-for"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2796..2822)
                     /label="pncas9-U-Kanam-Rv-2"
                     /note="sequence: aattcgatccattgctgttgacaaagg"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          2815..2846
                     /label="Kana-U-pncas9-Fw-2"
                     /note="sequence: atcgaattgtgtctcaaaatctctgatgttac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2916..2942
                     /label="Kanam-U-pnCas9-Fw-2"
                     /note="sequence: agcagcatgagccatattcagcgtgaa"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             2922..3737
                     /label="neo CDS"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     gene            2922..3737
                     /label="neo gene"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(3747..3772)
                     /label="Kanam-U-pncas9-rv"
                     /note="sequence: acgaattgacgcgcggaccgcggtcc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          3766..3791
                     /label="pncas9-U-Kanam-fw"
                     /note="sequence: aattcgttcaagccgagatcagcttc"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      4062..4413
                     /label="pRO1600 oriV"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      5431..5525
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          5432..5460
                     /label="AbR-USER-for"
                     /note="sequence: ACTCCTGTTGATAGATCCAGTAATGACCT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          5432..5460
                     /label="Primer071_AbR-USER-for"
                     /note="sequence: ACTCCTGTTGATAGATCCAGTAATGACCT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(5469..5495)
                     /label="Primer112_T0-pSEVA-U-rev"
                     /note="sequence: ACCGAGCGTTCTGAACAAATCCAGATG"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(5469..5495)
                     /label="312_DV_pSEVA-T0-U-rev"
                     /note="sequence: ACCGAGCGTTCTGAACAAATCCAGATG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(5473..5493)
                     /label="06< CRISPR_PCR"
                     /note="sequence: cgagcgttctgaacaaatcca"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(5473..5493)
                     /label="06< CRISPR_Rv pTn7"
                     /note="sequence: CGAGCGTTCTGAACAAATCCA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(5480..5500)
                     /label="Primer063_T0-terminator-seq-rev"
                     /note="sequence: CGGCAACCGAGCGTTCTGAAC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          5485..5501
                     /label="PS3"
                     /note="sequence: GAACGCTCGGTTGCCGC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          5485..5501
                     /label="PS6_227"
                     /note="sequence: GAACGCTCGGTTGCCGC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(5485..5501)
                     /label="Primer109_pS2"
                     /note="sequence: GCGGCAACCGAGCGTTC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5485..5501)
                     /label="PS2"
                     /note="sequence: GCGGCAACCGAGCGTTC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(5485..5501)
                     /label="102_pSEVA_PS2"
                     /note="sequence: GCGGCAACCGAGCGTTC"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     primer          complement(5485..5501)
                     /label="22-pSEVA_PS2"
                     /note="sequence: GCGGCAACCGAGCGTTC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(5485..5501)
                     /label="JT0073ps2"
                     /note="sequence: GCGGCAACCGAGCGTTC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(5485..5501)
                     /label="11_pS2"
                     /note="sequence: GCGGCAACCGAGCGTTC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(5485..5501)
                     /label="59- pS2"
                     /note="sequence: GCGGCAACCGAGCGTTC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          5487..5502
                     /label="Primer115_pSEVA-T0-U-for"
                     /note="sequence: ACGCTCGGTTGCCGCC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          5487..5502
                     /label="311_DV_pSEVA-T0-U-for"
                     /note="sequence: ACGCTCGGTTGCCGCC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5496..5525)
                     /label="Primer489_Prom_Term_U_rev"
                     /note="sequence: ATTCTCACCAATAAAAAACGCCCGGCGGCA"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          5592..5621
                     /label="ORIexc-UC-R"
                     /note="sequence: AGAAAAGATCAAAGGATCTTCTTGAGATCC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      5613..6201
                     /label="ori"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     6102..6121
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     6292..6311
                     /label="pRS-marker"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(6350..7771)
                     /label="SacB"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(6350..7771)
                     /label="Translation 7771-6350"
     primer          8123..8151
                     /label="pnCas9PABEC-UGI-U-for"
                     /note="sequence: ATGCTGTGAACTTCGGAATAGGAACTTCA"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        8151..8180
                     /label="trc promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          8153..8180
                     /label="Ptrc-seq-F"
                     /note="sequence: GACAATTAATCATCCGGCTCGTATAATG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          8153..8180
                     /label="Ptrc-seq-F"
                     /note="sequence: GACAATTAATCATCCGGCTCGTATAATG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    8193..8923
                     /label="Fragment1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     gene            8207..8923
                     /label="msfGFP"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(8896..8923)
                     /label="1- PEM7-msGFP-GA-F"
                     /note="sequence: TTATTTGTAGAGTTCATCCATGCCGTGC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          8931..8965
                     /label="Primer211_gRNA-U-std-rev"
                     /note="sequence: GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_RNA        8931..9006
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          8938..8971
                     /label="Primer205_gRNA-U-std-rev (pdhR)"
                     /note="sequence: AGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(8980..9004)
                     /label="156_U_sgRNA_accA_rev"
                     /note="sequence: ACCGACTCGGTGCCACTTTTTCAAG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     -35_signal      9041..9046
                     /label="trp"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        9041..9070
                     /label="The trc promoter combines the -35 region from the "
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     -10_signal      9064..9070
                     /label="lacUV5"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     protein_bind    9076..9096
                     /label="protein_bind"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     gene            9099..9116
                     /label="PA14_33310"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             9099..9116
                     /label="PA14_33310"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     source          complement(9099..9683)
                     /label="source"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             9120..9179
                     /label="Translation 9120-9179"
     gene            9180..9683
                     /label="PA14_33300"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             9180..9683
                     /label="PA14_33300"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             9180..9683
                     /label="Translation 9180-9683"
     CDS             9841..10527
                     /label="APOBEC-1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             join(10576..12179,1..2497)
                     /label="Cas9(D10A)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             join(10576..12179,1..2497)
                     /label="Translation 10576-2497"
     primer          11130..11148
                     /label="172_Cas9seq2"
                     /note="sequence: CCAGTTGGTACAAACCTAC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          11818..11836
                     /label="173_Cas9seq3"
                     /note="sequence: TTGGGTGAGCTGCATGCTA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 accagcattt ctttcaggtg aacagaagaa agccattgtt gatttactct tcaaaacaaa
       61 tcgaaaagta accgttaagc aattaaaaga agattatttc aaaaaaatag aatgttttga
      121 tagtgttgaa atttcaggag ttgaagatag atttaatgct tcattaggta cctaccatga
      181 tttgctaaaa attattaaag ataaagattt tttggataat gaagaaaatg aagatatctt
      241 agaggatatt gttttaacat tgaccttatt tgaagatagg gagatgattg aggaaagact
      301 taaaacatat gctcacctct ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta
      361 tactggttgg ggacgtttgt ctcgaaaatt gattaatggt attagggata agcaatctgg
      421 caaaacaata ttagattttt tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct
      481 gatccatgat gatagtttga catttaaaga agacattcaa aaagcacaag tgtctggaca
      541 aggcgatagt ttacatgaac atattgcaaa tttagctggt agccctgcta ttaaaaaagg
      601 tattttacag actgtaaaag ttgttgatga attggtcaaa gtaatggggc ggcataagcc
      661 agaaaatatc gttattgaaa tggcacgtga aaatcagaca actcaaaagg gccagaaaaa
      721 ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa gtcagattct
      781 taaagagcat cctgttgaaa atactcaatt gcaaaatgaa aagctctatc tctattatct
      841 ccaaaatgga agagacatgt atgtggacca agaattagat attaatcgtt taagtgatta
      901 tgatgtcgat cacattgttc cacaaagttt ccttaaagac gattcaatag acaataaggt
      961 cttaacgcgt tctgataaaa atcgtggtaa atcggataac gttccaagtg aagaagtagt
     1021 caaaaagatg aaaaactatt ggagacaact tctaaacgcc aagttaatca ctcaacgtaa
     1081 gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata aagctggttt
     1141 tatcaaacgc caattggttg aaactcgcca aatcactaag catgtggcac aaattttgga
     1201 tagtcgcatg aatactaaat acgatgaaaa tgataaactt attcgagagg ttaaagtgat
     1261 taccttaaaa tctaaattag tttctgactt ccgaaaagat ttccaattct ataaagtacg
     1321 tgagattaac aattaccatc atgcccatga tgcgtatcta aatgccgtcg ttggaactgc
     1381 tttgattaag aaatatccaa aacttgaatc ggagtttgtc tatggtgatt ataaagttta
     1441 tgatgttcgt aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata
     1501 tttcttttac tctaatatca tgaacttctt caaaacagaa attacacttg caaatggaga
     1561 gattcgcaaa cgccctctaa tcgaaactaa tggggaaact ggagaaattg tctgggataa
     1621 agggcgagat tttgccacag tgcgcaaagt attgtccatg ccccaagtca atattgtcaa
     1681 gaaaacagaa gtacagacag gcggattctc caaggagtca attttaccaa aaagaaattc
     1741 ggacaagctt attgctcgta aaaaagactg ggatccaaaa aaatatggtg gttttgatag
     1801 tccaacggta gcttattcag tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa
     1861 gttaaaatcc gttaaagagt tactagggat cacaattatg gaaagaagtt cctttgaaaa
     1921 aaatccgatt gactttttag aagctaaagg atataaggaa gttaaaaaag acttaatcat
     1981 taaactacct aaatatagtc tttttgagtt agaaaacggt cgtaaacgga tgctggctag
     2041 tgccggagaa ttacaaaaag gaaatgagct ggctctgcca agcaaatatg tgaatttttt
     2101 atatttagct agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca
     2161 attgtttgtg gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt
     2221 ttctaagcgt gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa
     2281 acatagagac aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac
     2341 gaatcttgga gctcccgctg cttttaaata ttttgataca acaattgatc gtaaacgata
     2401 tacgtctaca aaagaagttt tagatgccac tcttatccat caatccatca ctggtcttta
     2461 tgaaacacgc attgatttga gtcagctagg aggtgactcc ggcggctcca ccaacctgtc
     2521 cgacatcatc gagaaggaga ctggcaagca gctggtcatc caggagtcca tcctgatgct
     2581 gccggaggaa gtcgaggaag tcatcggcaa caagcccgag tccgacatcc tggtccacac
     2641 cgcctacgac gagtccaccg acgagaacgt catgctgctg acctcggacg cccccgagta
     2701 caagccctgg gccctggtca tccaggactc caacggcgag aacaagatca agatgctgtg
     2761 aacttcggaa taggaacttc aagatcccct gattcccttt gtcaacagca atggatcgaa
     2821 ttgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     2881 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcagcgtg
     2941 aaacgagctg tagccgtccg cgtctgaaca gcaacatgga tgcggatctg tatggctata
     3001 aatgggcgcg tgataacgtg ggtcagagcg gcgcgaccat ttatcgtctg tatggcaaac
     3061 cggatgcgcc ggaactgttt ctgaaacatg gcaaaggcag cgtggcgaac gatgtgaccg
     3121 atgaaatggt gcgtctgaac tggctgaccg aatttatgcc gctgccgacc attaaacatt
     3181 ttattcgcac cccggatgat gcgtggctgc tgaccaccgc gattccgggc aaaaccgcgt
     3241 ttcaggtgct ggaagaatat ccggatagcg gcgaaaacat tgtggatgcg ctggccgtgt
     3301 ttctgcgtcg tctgcatagc attccggtgt gcaactgccc gtttaacagc gatcgtgtgt
     3361 ttcgtctggc ccaggcgcag agccgtatga acaacggcct ggtggatgcg agcgattttg
     3421 atgatgaacg taacggctgg ccggtggaac aggtgtggaa agaaatgcat aaactgctgc
     3481 cgtttagccc ggatagcgtg gtgacccacg gcgattttag cctggataac ctgattttcg
     3541 atgaaggcaa actgattggc tgcattgatg tgggccgtgt gggcattgcg gatcgttatc
     3601 aggatctggc cattctgtgg aactgcctgg gcgaatttag cccgagcctg caaaaacgtc
     3661 tgtttcagaa atatggcatt gataatccgg atatgaacaa actgcaattt catctgatgc
     3721 tggatgaatt tttctaataa ttaattggac cgcggtccgc gcgtcaattc gttcaagccg
     3781 agatcagctt cccggccgcg gagttgttcg gtaaattgtc acaacgccgc ggccaattcg
     3841 agagttttcg ttccactgag cgtcagaccc caattacacg ccactggctg tgcttgctgg
     3901 ggtgacggtg gcaacggtgg cggccttgct gggctatcgc gttggaaaga aacgagggaa
     3961 aggggactga taaaccggtc ttagcccctc cccttggtgt ccaaccgctc tgtaggcctc
     4021 tcaggcgccg ctggtgccgc tggttggacg ccaagggtga atccgcctcg ataccctgat
     4081 tactcgcttc ctgcgccctc tcaggcggcg ataggggact ggtaaaacgg ggattgccca
     4141 gacgcctccc ccgccccttc aggggcacaa atgcggcccc aacggggcca cgtagtggtg
     4201 cgttttttgc gtttccaccc ttttcttcct tttccctttt aaacctttta ggacgtctac
     4261 aggccacgta atccgtggcc tgtagagttt aaaaagggac ggatttgttg ccattaaggg
     4321 acggatttgt tgttaagaag ggacggattt gttgttgtaa agggacggat ttgttgtatt
     4381 gtgggacgca gatacagtgt ccccttatac acaaggaatg tcgaacgtgg cctcaccccc
     4441 aatggtttac aaaagcaatg ccctggtcga ggccgcgtat cgcctcagtg ttcaggaaca
     4501 gcggatcgtt ctggcctgta ttagccaggt gaagaggagc gagcctgtca ccgatgaagt
     4561 gatgtattca gtgacggcgg aggacatagc gacgatggcg ggtgtcccta tcgaatcttc
     4621 ctacaaccag ctcaaagaag cggccctgcg cctgaaacgg cgggaagtcc ggttaaccca
     4681 agagcccaat ggcaagggga aaagaccgag tgtgatgatt accggctggg tgcaaacaat
     4741 catctaccgg gagggtgagg gccgtgtaga actcaggttc accaaagaca tgctgccgta
     4801 cctgacggaa ctcaccaaac agttcaccaa atacgccttg gctgacgtgg ccaagatgga
     4861 cagcacccac gcgatcaggc tttacgagct gctcatgcaa tgggacagca tcggccagcg
     4921 cgaaatagaa attgaccagc tgcgaaagtg gtttcaactg gaaggccggt atccctcgat
     4981 caaggacttc aagttgcgag tgcttgatcc agccgtgacg cagatcaacg agcacagccc
     5041 gctacaggtg gagtgggcgc agcgaaagac cgggcgcaag gtcacacatc tgttgttcag
     5101 ttttggaccg aagaagcccg ccaaggcggt gggtaaggcc ccagcgaagc gcaaggccgg
     5161 gaagatttca gatgctgaga tcgcgaaaca ggctcgccct ggtgagacat gggaagcggc
     5221 ccgcgctcga ctaacccaga tgccgcttgc gcaaactatt aactggcgaa ctacttactc
     5281 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5341 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5401 gcgggactct ggggttcgag agctcgcttg gactcctgtt gatagatcca gtaatgacct
     5461 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg
     5521 agaatccaag cactagtcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5581 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5641 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5701 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     5761 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5821 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5881 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5941 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6001 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6061 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6121 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6181 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6241 tgctggcctt ttgctcacat gttctttcct gcgttgtcga cagagcccgg gcggcatcag
     6301 agcagattgt actgagagtg caccataatc ggcattttct tttgcgtttt tatttgttaa
     6361 ctgttaattg tccttgttca aggatgctgt ctttgacaac agatgttttc ttgcctttga
     6421 tgttcagcag gaagctaggc gcaaacgttg attgtttgtc tgcgtagaat cctctgtttg
     6481 tcatatagct tgtaatcacg acattgtttc ctttcgcttg aggtacagcg aagtgtgagt
     6541 aagtaaaggt tacatcgtta ggatcaagat ccatttttaa cacaaggcca gttttgttca
     6601 gcggcttgta tgggccagtt aaagaattag aaacataacc aagcatgtaa atatcgttag
     6661 acgtaatgcc gtcaatcgtc atttttgatc cgcgggagtc agtgaacaga taccatttgc
     6721 cgttcatttt aaagacgttc gcgcgttcaa tttcatctgt tactgtgtta gatgcaatca
     6781 gcggtttcat cacttttttc agtgtgtaat catcgtttag ctcaatcata ccgagagcgc
     6841 cgtttgctaa ctcagccgtg cgttttttat cgctttgcag aagtttttga ctttcttgac
     6901 ggaagaatga tgtgcttttg ccatagtatg ctttgttaaa taaagattct tcgccttggt
     6961 agccatcttc agttccagtg tttgcttcaa atactaagta tttgtggcct ttatcttcta
     7021 cgtagtgagg atctctcagc gtatggttgt cgcctgagct gtagttgcct tcatcgatga
     7081 actgctgtac attttgatac gtttttccgt caccgtcaaa gattgattta taatcctcta
     7141 caccgttgat gttcaaagag ctgtctgatg ctgatacgtt aacttgtgca gttgtcagtg
     7201 tttgtttgcc gtaatgttta ccggagaaat cagtgtagaa taaacggatt tttccgtcag
     7261 atgtaaatgt ggctgaacct gaccattctt gtgtttggtc ttttaggata gaatcatttg
     7321 catcgaattt gtcgctgtct ttaaagacgc ggccagcgtt tttccagctg tcaatagaag
     7381 tttcgccgac tttttgatag aacatgtaaa tcgatgtgtc atccgcattt ttaggatctc
     7441 cggctaatgc aaagacgatg tggtagccgt gatagtttgc gacagtgccg tcagcgtttt
     7501 gtaatggcca gctgtcccaa acgtccaggc cttttgcaga agagatattt ttaattgtgg
     7561 acgaatcgaa ctcaggaact tgatattttt catttttttg ctgttcaggg atttgcagca
     7621 tatcatggcg tgtaatatgg gaaatgccgt atgtttcctt atatggcttt tggttcgttt
     7681 ctttcgcaaa cgcttgagtt gcgcctcctg ccagcagtgc ggtagtaaag gttaatactg
     7741 ttgcttgttt tgcaaacttt ttgatgttca tcgttcatgt ctcctttttt atgtactgtg
     7801 ttagcggtct gcttcttcca gccctcctgt ttgaagatgg caagttagtt acgcacaata
     7861 aaaaaagacc taaaatatgt aaggggtgac gccaaagtat acactttgcc ctttacacat
     7921 tttaggtctt gcctgcttta tcagtaacaa acccgcgcga tttacttttc gacctcattc
     7981 tattagactc tcgtttggat tgcaactggt ctattttcct cttttgtttg atagaaaatc
     8041 ataaaaggat ttgcagacta cgggcctaaa gaactaaaaa atctatctgt ttcttttcat
     8101 tctctgtatt ttttatagtt tctgttgcat gggcataaag ttgccttaag ttgacaatta
     8161 atcatccggc tcgtataatg tgtggcgaga ccAGGAGGAA AAACATAtgc gtAAAGGTGA
     8221 AGAACTGTTC ACCGGTGTTG TTCCGATCCT GGTTGAACTG GATGGTGATG TTAACGGCCA
     8281 CAAATTCTCT GTTCGTGGTG AAGGTGAAGG TGATGCAACC AACGGTAAAC TGACCCTGAA
     8341 ATTCATCTGC ACTACCGGTA AACTGCCGGT TCCATGGCCG ACTCTGGTGA CTACCCTGAC
     8401 CTATGGTGTT CAGTGTTTTT CTCGTTACCC GGATCACATG AAGCAGCATG ATTTCTTCAA
     8461 ATCTGCAATG CCGGAAGGTT ATGTACAGGA GCGCACCATT TCTTTCAAAG ACGATGGCAC
     8521 CTACAAAACC CGTGCAGAGG TTAAATTTGA AGGTGATACT CTGGTGAACC GTATTGAACT
     8581 GAAAGGCATT GATTTCAAAG AGGACGGCAA CATCCTGGGC CACAAACTGG AATATAACTT
     8641 CAACTCCCAT AACGTTTACA TCACCGCAGA CAAACAGAAG AACGGTATCA AAGCTAACTT
     8701 CAAAATTCGC CATAACGTTG AAGACGGTAG CGTACAGCTG GCGGACCACT ACCAGCAGAA
     8761 CACTCCGATC GGTGATGGTC CGGTTCTGCT GCCGGATAAC CACTACCTGT CCACCCAGTC
     8821 TAAACTGTCC AAAGACCCGA ACGAAAAGCG CGACCACATG GTGCTGCTGG AGTTCGTTAC
     8881 TGCcGCAGGT ATCACGCACG GCATGGATGA ACTCTACAAA TAAggtctca gttttagagc
     8941 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     9001 cggtgctttt tttgagatct gtccataccc atggtctaga TTGACAATTA ATCATCCGGC
     9061 TCGTATAATG TGTGGAATTG TGAGCGGATA ACAATTTCga agccgaagag aagtaagcca
     9121 tggaccacta cctcgacatt cgcttgcgac cggacccgga atttcccccg gcgcaactca
     9181 tgagcgtgct cttcggcaag ctccaccagg ccctggtggc acagggcggg gacaggatcg
     9241 gcgtgagctt ccccgacctc gacgaaagcc gctcccggct gggcgagcgc ctgcgcattc
     9301 atgcctcggc ggacgacctt cgtgccctgc tcgcccggcc ctggctggaa gggttgcggg
     9361 accatctgca attcggagaa ccggcagtcg tgcctcaccc cacaccgtac cgtcaggtca
     9421 gtcgggttca ggcgaaaagc aatccggaac gcctgcggcg gcggctcatg cgccggcacg
     9481 atctgagtga ggaggaggct cggaaacgca ttcccgatac ggtcgcgaga gccttggacc
     9541 tgcccttcgt cacgctacgc agccagagca ccggacagca cttccgtctc ttcatccgcc
     9601 acgggccgtt gcaggtgacg gcagaggaag gaggattcac ctgttacggg ttgagcaaag
     9661 gaggtttcgt tccctggttc tgactcgagg catcaacgac aagccccggc tatcccgata
     9721 gccggggctt tgtcttgacg gtgtacaaga acctctttac tattttgtac cccgaaaatt
     9781 ggcagggcgc acgctctgtc atttcgtttg tgtgtcgtaa gacaatcagt ggagctatag
     9841 atgagtagcg aaaccggtcc ggttgcagtg gatccgaccc tgcgccgtcg cattgaaccg
     9901 cacgagtttg aagtgttctt tgatccgcgc gagctgcgca aagaaacttg cctgctgtac
     9961 gagattaact ggggtggccg ccatagcatc tggcgccata ccagccagaa caccaataag
    10021 cacgtggaag tgaattttat tgaaaaattt accaccgagc gctacttctg ccctaatacc
    10081 cgctgcagca tcacctggtt tctgagctgg agcccgtgcg gcgaatgtag tcgcgccatt
    10141 accgagttcc tgagccgcta tccgcatgtg accctgttca tctacatcgc ccgtctgtac
    10201 catcacgccg atccgcgcaa tcgccaaggt ctgcgtgatc tgattagcag cggtgtgacc
    10261 atccagatca tgaccgaaca agagagcggc tattgctggc gcaacttcgt gaactattct
    10321 ccgagcaacg aagcccactg gccgcgttat ccgcatctgt gggtgcgcct gtatgtgctg
    10381 gagctgtact gcatcatcct gggcctgccg ccttgcctga atattctgcg ccgtaaacag
    10441 ccgcaactga cattcttcac catcgcactg cagagctgcc attatcagcg cctgccgccg
    10501 cacattttat gggccaccgg tctgaaaagc ggtagtgaaa ctccgggcac aagcgaaagc
    10561 gcaaccccgg aaagtgataa gaaatactca ataggcttag ctatcggcac aaatagcgtc
    10621 ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa ggttctggga
    10681 aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga
    10741 gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac acgtcggaag
    10801 aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt
    10861 ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat
    10921 cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat
    10981 catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg
    11041 gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct
    11101 gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt
    11161 gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg
    11221 agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc
    11281 ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt
    11341 gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat
    11401 aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta
    11461 tcagatgcta ttttactttc agatatccta agagtaaata ctgaaataac taaggctccc
    11521 ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa
    11581 gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa
    11641 aacggatatg caggttatat tgatggggga gctagccaag aagaatttta taaatttatc
    11701 aaaccaattt tagaaaaaat ggatggtact gaggaattat tggtgaaact aaatcgtgaa
    11761 gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg
    11821 ggtgagctgc atgctatttt gagaagacaa gaagactttt atccattttt aaaagacaat
    11881 cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt attatgttgg tccattggcg
    11941 cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg
    12001 aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca
    12061 aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag
    12121 tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaagg aatgcgaaa
//