LOCUS       Exported               11052 bp ds-DNA     circular SYN 14-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   VB211122-1170cgc
VERSION     .
KEYWORDS    pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="synthetic DNA construct"
     promoter        1..229
                     /label=RSV promoter
                     /label=RSV promoter
                     /note="/application_notes=Strong promoter; drives 
                     transcription of viral RNA in packaging cells."
                     /note="/description=Rous sarcoma virus enhancer/promoter"
                     /note="/full_name=Rous sarcoma virus (RSV) 
                     enhancer/promoter"
                     /note="/official_designation=RSV"
                     /note="/uuid=3f2ddca7f0cb4015b6abcec8a25c834e"
     LTR             230..410
                     /label=5' LTR-Delta-U3
                     /note="/application_notes=Allows transcription of viral RNA
                     and its packaging into virus."
                     /note="/description=Truncated HIV-1 5' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 5' LTR"
                     /note="/official_designation=Delta5' LTR"
                     /note="/uuid=44156a3083604321be44066133e8ecb9"
     misc_feature    521..565
                     /label=-Psi
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus."
                     /note="/description=HIV-1 packaging signal"
                     /note="/full_name=HIV-1 psi packaging signal"
                     /note="/official_designation=Psi"
                     /note="/uuid=284899413ff943a99f8210a11ad8c54e"
     misc_feature    1075..1308
                     /label=RRE
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Rev protein binding site that 
                     allows Rev-dependent nuclear export of viral RNA during 
                     viral packaging."
                     /note="/description=HIV-1 Rev response element"
                     /note="/full_name=HIV-1 Rev response element"
                     /note="/official_designation=RRE"
                     /note="/uuid=df07099e49674cf3aba3661b54e28bd7"
     misc_feature    1803..1920
                     /label=cPPT
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Facilitates the nuclear import of
                     HIV-1 cDNA through a central DNA flap."
                     /note="/description=Central polypurine tract"
                     /note="/full_name=Central polypurine tract"
                     /note="/official_designation=cPPT"
                     /note="/uuid=f7cb29985f9f41a7a5924d3462266f42"
     promoter        1950..3128
                     /label=EF1A
                     /note="/application_notes=Strong promoter."
                     /note="/description=Human eukaryotic translation elongation
                     factor 1 alpha1 promoter"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor 1 alpha1 promoter"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
     misc_feature    3153..3158
                     /label=Kozak
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Facilitates translation 
                     initiation of ATG start codon downstream of the Kozak 
                     sequence."
                     /note="/description=Kozak translation initiation sequence"
                     /note="/full_name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
     misc_feature    3159..4766
                     /label={WWE(A)(R163A)-HA-nLuc-FLAG}
                     /label=nonstandard type: ORF
                     /note="/application_notes="
                     /note="/official_designation={WWE(A)(R163A)-HA-nLuc-FLAG}"
                     /note="/uuid=940524daf0be4cb880301ed624e561b4"
     misc_feature    4767..4829
                     /label=T2A
                     /label=nonstandard type: Linker
                     /note="/application_notes=Causes co-translational cleavage 
                     of the encoded polypeptide. Multiple proteins can be made 
                     from a polycistronic transcript containing multiple ORFs 
                     separated by 2A. P2A and T2A have higher cleavage 
                     efficiency compared to other 2As."
                     /note="/description=Self-cleaving 2A peptide from Thosea 
                     asigna virus"
                     /note="/full_name=Thosea asigna virus 2A peptide"
                     /note="/official_designation=T2A"
                     /note="/uuid=995274d0c39f4cbdbab66fb78aa938d6"
     misc_feature    4830..5651
                     /label={WWE(B)-myc-cLuc-Flag}
                     /label=nonstandard type: ORF
                     /note="/application_notes="
                     /note="/official_designation={WWE(B)-myc-cLuc-Flag}"
                     /note="/uuid=f559546893864bf88d64d713338a3d0c"
     misc_feature    5676..6263
                     /label=IRES
                     /label=nonstandard type: Linker
                     /note="/application_notes=Recruits ribosome to initiate 
                     translation internally on a transcript independent of its 
                     5' end. Multiple proteins can be made from a polycistronic 
                     transcript containing multiple ORFs separated by IRES."
                     /note="/description=Encephalomyocarditis virus internal 
                     ribosome entry site"
                     /note="/full_name=Encephalomyocarditis virus internal 
                     ribosome entry site"
                     /note="/official_designation=IRES"
                     /note="/uuid=344233c15e2a4a5a9566583bfc1af3cb"
     misc_feature    6264..6863
                     /label=Puro
                     /label=nonstandard type: ORF
                     /note="/application_notes=Allows cells to be resistant to 
                     puromycin."
                     /note="/description=Puromycin resistance gene"
                     /note="/full_name=Puromycin resistance gene"
                     /note="/official_designation=Puro"
                     /note="/uuid=39d7413208494d9291d10b21b13ca44c"
     misc_feature    6893..7490
                     /label=WPRE
                     /label=nonstandard type: Miscellaneous
                     /note="/application_notes=Enhances virus stability in 
                     packaging cells, leading to higher titer of packaged virus;
                     enhances higher expression of transgenes."
                     /note="/description=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/full_name=Woodchuck hepatitis virus 
                     posttranscriptional regulatory element"
                     /note="/official_designation=WPRE"
                     /note="/uuid=0aedff8308474b369c622433538d7836"
     LTR             7572..7806
                     /label=3' LTR-Delta-U3
                     /note="/application_notes=Allows packaging of viral RNA 
                     into virus; self-inactivates the 5' LTR by a copying 
                     mechanism during viral genome integration; contains 
                     polyadenylation signal for transcription termination."
                     /note="/description=Truncated HIV-1 3' long terminal 
                     repeat"
                     /note="/full_name=HIV-1 truncated 3' LTR"
                     /note="/official_designation=DeltaU3/3' LTR"
                     /note="/uuid=c958755414c34372b909e20e81ebcef6"
     polyA_signal    7879..8013
                     /label=SV40 early pA
                     /note="/application_notes=Allows transcription termination 
                     and polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Simian virus 40 early polyadenylation 
                     signal"
                     /note="/full_name=SV40 early polyadenation signal"
                     /note="/official_designation=SV40 early pA"
                     /note="/uuid=2fcb13a62e704d8095b188d9625a6a94"
     misc_feature    8967..9827
                     /label=AmpiciIIin
                     /label=nonstandard type: ORF
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
     rep_origin      9998..10586
                     /direction=RIGHT
                     /label=pUC ori
                     /note="/application_notes=Facilitates plasmid replication 
                     in E. coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=64a8cb3def9f49deb6752795c2d893a8"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtatca actttgtata gaaaagttgg gctccggtgc ccgtcagtgg gcagagcgca
     1981 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2041 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2101 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2161 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2221 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     2281 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     2341 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     2401 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     2461 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     2521 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     2581 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     2641 ctcaagctgg ccggcctgct ctggtgcctg gtctcgcgcc gccgtgtatc gccccgccct
     2701 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     2761 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     2821 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     2881 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     2941 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     3001 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     3061 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     3121 tgtcgtgaca agtttgtaca aaaaagcagg ctgccaccat gggaaatggt gaatatgcat
     3181 ggtattatga aggaagaaat gggtggtggc agtacgatga gcgcactagt agagagctgg
     3241 aagatgcttt ttccaaaggt aaaaagaaca ctgaaatgtt aattgctggc tttctgtatg
     3301 tcgctgatct tgaaaacatg gttcaatata ggagaaatga acatggacgt cgcgctaaga
     3361 ttaagcgaga tataatagat ataccaaaga agggagtagc tggacttagg atctccggac
     3421 agatcagcta cgccagccgg ggcggaggta gctctggcgg aggtgcctac ccatacgatg
     3481 ttccagatta cgctgcagaa gatgccaaaa acattaagaa gggcccagcg ccattctacc
     3541 cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg
     3601 tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt
     3661 acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc
     3721 atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc
     3781 tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga
     3841 acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga
     3901 tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga
     3961 ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct
     4021 tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca
     4081 tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt
     4141 gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg
     4201 ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact
     4261 tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca
     4321 gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg
     4381 ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg
     4441 gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca
     4501 tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg
     4561 acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact
     4621 tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca
     4681 tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg
     4741 gcgactacaa ggacgacgat gacaagggaa gcggagaggg caggggaagt cttctaacat
     4801 gcggggacgt ggaggaaaat cccggcccca tgggcaacgg cgagtatgca tggtattacg
     4861 aaggcagaaa cggatggtgg caatatgatg aaagaacttc tcgagaactg gaagatgcgt
     4921 tctctaaagg gaagaagaat actgaaatgt taatagcggg atttctttac gtcgccgact
     4981 tagaaaacat ggtgcaatac cggcgaaatg agcacggccg gagaaggaaa ataaagaggg
     5041 atattataga tatccccaag aaaggtgtgg ctgggttaag gtcccagatc agctacgcca
     5101 gccggggcgg aggtagctct ggcggaggtg ccgagcagaa gctgatcagc gaggaagacc
     5161 tgtccggcag cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg
     5221 gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg
     5281 accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga
     5341 gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg
     5401 atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga
     5461 aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg
     5521 ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc
     5581 gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtggactac aaggacgacg
     5641 atgacaagtg aacccagctt tcttgtacaa agtgggcccc tctccctccc ccccccctaa
     5701 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     5761 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     5821 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     5881 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     5941 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     6001 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     6061 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     6121 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     6181 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     6241 acgatgataa tatggccaca accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     6301 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     6361 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     6421 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     6481 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6541 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6601 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6661 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     6721 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     6781 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     6841 tgacccgcaa gcccggtgcc tgacaacttt attatacata gttgatcaat tccgataatc
     6901 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6961 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7021 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7081 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7141 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7201 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7261 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     7321 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7381 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7441 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcgg gaattcccgc
     7501 ggttcgcttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7561 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     7621 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7681 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7741 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7801 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7861 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7921 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7981 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     8041 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8101 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8161 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     8221 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     8281 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     8341 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     8401 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     8461 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8521 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8581 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8641 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8701 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8761 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     8821 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8881 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8941 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     9001 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     9061 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     9121 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     9181 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     9241 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     9301 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9361 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9421 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9481 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9541 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9601 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9661 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9721 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9781 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9841 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9901 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9961 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    10021 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    10081 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    10141 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    10201 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    10261 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10321 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    10381 agcgtgagct atgagaaagc gccacgcttc ccgaagagag aaaggcggac aggtatccgg
    10441 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    10501 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    10561 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    10621 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10681 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10741 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10801 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    10861 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    10921 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    10981 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    11041 agctgcaagc tt
//
