LOCUS       Exported                5883 bp ds-DNA     circular SYN 22-JUN-2022
DEFINITION  Sobol_C5S_1_422 coverage: 600x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 22, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(32..56)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(94..112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(157..360)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        361..740
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1006..1110
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1111..1971
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2142..2730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3018..3039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3054..3084
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3092..3108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3116..3132
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3258..3482
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4257..4829
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     intron          4901..5130
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     protein_bind    5230..5254
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(5272..5883,1))
                     /codon_start=1
                     /label=MGMT
                     /translation="IVK*NAPHWTALWGSWSCLVVSRVCTK*SSWARGRLQLMPWRSQP
                     PLRFSEVRSP*CSAQPG*MPISTSPRLSKSSPCRLFTIPFSSKSRSPDRCYGSC*RL*N
                     SEK*FLTSN*QPWQATPKPREQWEEQ*EAILSPSSSRATEWSAAAEPWATTPEDWP*RN
                     GFWPMKATGWGSQAWEGAQVWQGPGSRERELPRAPRLLAET"
ORIGIN
        1 tccttgtcca tggtgaaggg ggcggccgcg gagcctgctt ttttgtacaa acttgttgat
       61 atcgatccga gctcggtacc aagcttgggt ctccctatag tgagtcgtat taatttcgat
      121 aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat agacctccca
      181 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
      241 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
      301 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
      361 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
      421 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
      481 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
      541 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
      601 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
      661 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
      721 actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa
      781 acgcctaacc ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc
      841 ttaaattttg ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg
      901 gcttaactat gcggcatcag agcagattgt actgagagtg caccataggg gatcgggaga
      961 tctcccgatc cgtcgacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1021 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1081 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1141 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1201 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1261 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1321 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1381 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1441 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1501 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1561 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1621 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1681 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1741 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1801 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1861 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1921 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1981 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2041 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2101 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2161 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2221 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2281 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2341 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2401 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2461 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2521 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2581 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2641 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2701 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2761 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2821 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2881 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2941 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3001 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     3061 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     3121 aacagctatg accatgatta cgccaagctc tagctagagg tcgacggtat acagacatga
     3181 taagatacat tgatgagttt ggacaaacca caactagaat gcactcgagc cccagctggt
     3241 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
     3301 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
     3361 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
     3421 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
     3481 agtcgaggct gatcagcgag ctctagagtc ggtgggcctc gggggcgggt gcggggtcgg
     3541 cggggccgcc ccgggtggct tcggtcggag ccatggggtc gtgcgctcct ttcggtcggg
     3601 cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc gggtcatgca ccaggtgcgc
     3661 ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc cgagccgctc gtagaagggg
     3721 aggttgcggg gcgcggaggt ctccgaggaa ggcgggcacc ccggcgcgct cggccgcctc
     3781 cactccgggg agcacgacgg cgctgcccag acccttgccc tggtggtcgg gcgagacgcc
     3841 gacggtggcc gggaaccacg cgggctcctt gggccggtgc ggcgccagga ggccttccat
     3901 ctgttgctgc gcggccagcc gggaaccgct caactcggcc atgcgcgggc cgatctcggc
     3961 gaacaccgcc cccgcttcga cgctctccgg cgtggtccag accgccaccg cggcgccgtc
     4021 gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag
     4081 ctcggtgacc cgctcgatgt ggcggtccgg gtcgacggtg tggcgcgtgg cggggtagtc
     4141 ggcgaacgcg gcggcgaggg tgcgtacggc ccgggggacg tcgtcgcggg tggcgaggcg
     4201 caccgtgggc ttgtactcgg tcatggaagg tcgtctcctt gtgggttgtg gcaagcttat
     4261 catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag acgttttttt
     4321 aacctcgact aaacacatgt aaagcatgtg caccgaggcc ccagatcaga tcccatacaa
     4381 tggggtacct tctgggcatc cttcagcccc ttgttgaata cgcttgagga gagccatttg
     4441 actctttcca caactatcca actcacaacg tggcactggg gttgtgccgc ctttgcaggt
     4501 gtatcttata cacgtggctt ttggccgcag aggcacctgt cgccaggtgg ggggttccgc
     4561 tgcctgcaaa gggtcgctac agacgttgtt tgtcttcaag aagcttccag aggaactgct
     4621 tccttcacga cattcaacag accttgcatt cctttggcga gaggggaaag acccctagga
     4681 atgctcgtca agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat
     4741 atggtggaaa ataacatata gacaaacgca caccggcctt attccaagcg gcttcggcca
     4801 gtaacgttag ggggggggag ggagaggggg ggggggagag gggcggaatt ggccgcccta
     4861 gatgcatgct cgacctgcag ttggacctgg gagtggacac ctgtggagag aaaggcaaag
     4921 tggatgtcat tgtcactcaa gtgtatggcc agatctcaag cctgccacac ctcaagcttg
     4981 acaacaaaaa gattgtcttt tctgaccaga tggacgcggc caccctcaaa ggcatcaccg
     5041 cgggccaggt gaatatcaaa tcctcctcgt ttttggaaac tgacaatctt agcgcagaag
     5101 tcatgcccgc ttttgagagg gagtactcac cccaacagct ggccctcgca gacagcgaat
     5161 taattccagc acactggcgg ccgttactag tggatccact gaattccgtc gacgcggccg
     5221 cagatatcaa ccactttgta caagaaagct gggtcggcgc gcccaccctt ctagtttcgg
     5281 ccagcaggcg gggagcccga ggtagctccc gctcccttga gccaggcccc tgccagacct
     5341 gagctccctc ccaagcctgg cttccccaac cggtggcctt catgggccag aagccattcc
     5401 ttcacggcca gtcctccgga gtagttgccc acggctccgc tgctgcagac cactctgtgg
     5461 cacgggatga ggatggggac aggattgcct ctcattgctc ctcccactgc tcgcgcggct
     5521 ttggggttgc ctgccagggc tgctaattgc tggtaagaaa tcacttctcc gaatttcaca
     5581 accttcagca gcttccataa cacctgtctg gtgaacgact cttgctggaa aacgggatgg
     5641 tgaagagccg gcacggggaa ctcttcgata gcctcgggct ggtggaaata ggcattcagc
     5701 caggctgtgc actgcatcag gggctccgga cctccgagaa ccgcagcggg ggctgggacc
     5761 tccacggcat cagctgcaga cgtccccttg cccaggagct ttatttcgtg cagaccctgc
     5821 tcacaaccag acagctccag cttccccaaa gggctgtcca gtgtggtgcg tttcatttca
     5881 caa
//
