LOCUS       pcDNA-mSUN1\EGFP        8884 bp    DNA     circular     24-JAN-2019
DEFINITION  attB1-attB2-attB5.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-12652321|
COMMENT     VNTDBDATE|-12655029|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pcDNA-mSUN1 EGFP|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pcDNA-mSUN1 EGFP" 0 8884 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 -65415 0 "" 0 "" "" "" "" "" "" "pcDNA6.2/V5-DEST" 
COMMENT             "" 0 0 1 5099 1 5099 3162 919 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 2 0 3162 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 919 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 -2147418488 0 "" 0 "" "" "" "" "" "" 
COMMENT             "pE-mSUN1 L1R5" "" 0 0 1 2769 5100 7868 2583 5351 0 "" "" "" "" "" 
COMMENT             "" (CObList) (CTerm 2 0 2583 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 5351 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "pE(2F)EGFPpA (L5-L2)" 
COMMENT             "" 0 0 1 1016 7869 8884 2531 3546 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 2 0 2531 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 3546 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI" "AgeI" "ApoI" "AscI" "AvaII" "BglI" "BglII" 
COMMENT         "EcoRV" "KpnI" "NdeI" "NheI" "NotI" "PmeI" "PvuI" "PvuII" "SacI" 
COMMENT         "SacII" "SalI" "ScaI" "SpeI" "XbaI" "XhoI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 8884 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #3=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 2682 0 3925 0 4213 0 0 
COMMENT            "") 
COMMENT        #4=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 7 1193 0 5463 0 6261 0 6531 
COMMENT            0 7096 0 7472 0 8632 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 8619 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 7114 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 6 1079 0 1299 0 4791 0 5120 
COMMENT            0 6125 0 7583 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "PstI" "ctgcag" 6 0 5 5686 0 6755 0 7151 0 
COMMENT             7187 0 8652 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 1195 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 1193 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AgeI" "accggt" 2 0 2 86 0 7829 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "ApoI" "raatty" 2 0 5 829 0 1907 0 4308 0 8619 
COMMENT             0 8757 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 9 230 0 1460 0 3396 0 3618 
COMMENT             0 5562 0 5738 0 7443 0 7788 0 8541 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 4 3378 0 4521 0 4643 
COMMENT             0 4714 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "BglII" "agatct" 2 0 4 381 0 4193 0 7562 0 
COMMENT             8611 0 0 "") #19=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 2 6297 0 7539 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 4665 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 1 104 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 1536 0 3628 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "PvuII" "cagctg" 4 0 5 392 0 1508 0 2192 0 
COMMENT             6993 0 7022 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "SacI" "gagctc" 6 0 2 4999 0 7784 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 32 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 1994 0 4179 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 3738 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 4430 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 19 0 8640 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 4 5463 0 7096 0 7472 0 
COMMENT             8632 0 0 "") 
COMMENT        #34=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 4 844 0 3857 0 6845 0 
COMMENT             7592 0 0 "")) 
COMMENT       (CObList 
COMMENT        #35=(CFSignal (CObList) "attB1" 21 0 0 5092 5112 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #36=(CFSignal (CObList) "attB2" 21 0 1 8877 13 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #37=(CFSignal (CObList) "attB5" 21 0 0 7861 7881 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #38=(CFSignal (CObList) "mSUN1 no stop" 4 0 0 5121 7859 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #39=(CFSignal (CObList) "3 stops" 4 0 0 91 99 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"123000001\"") 1 1 1 1 "") 
COMMENT        #40=(CFSignal (CObList) "V5 epitope" 4 0 0 40 81 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1350000\"") 1 1 1 1 "") 
COMMENT        #41=(CFSignal (CObList) "V5 reverse primer" 27 0 1 49 69 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2140000\"") 1 1 1 1 "") 
COMMENT        #42=(CFSignal (CObList) "TK pA reverse primer" 27 0 1 115 133 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"2120000\"") 1 1 1 1 "") 
COMMENT        #43=(CFSignal (CObList) "TK pA" 25 0 0 108 379 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1860000\"") 1 1 1 1 "") 
COMMENT        #44=(CFSignal (CObList) "T7 promoter" 30 0 0 5043 5059 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2370000\"") 1 1 1 1 "") 
COMMENT        #45=(CFSignal (CObList) "T7 primer" 27 0 0 5043 5062 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2100000\"") 1 1 1 1 "") 
COMMENT        #46=(CFSignal (CObList) "SV40 pA" 25 0 0 1856 1986 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1840000\"") 1 1 1 1 "") 
COMMENT        #47=(CFSignal (CObList) "SV40 early promoter" 29 0 0 848 1217 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"2290000\"") 1 1 1 1 "") 
COMMENT        #48=(CFSignal (CObList) "pUC origin" 33 0 1 2369 3039 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2540000\"") 1 1 1 1 "") 
COMMENT        #49=(CFSignal (CObList) "f1 origin" 33 0 0 415 843 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2500000\"") 1 1 1 1 "") 
COMMENT        #50=(CFSignal (CObList) "EM7 promoter" 30 0 0 1232 1299 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2340000\"") 1 1 1 1 "") 
COMMENT        #51=(CFSignal (CObList) "CMV promoter" 29 0 0 4412 4999 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2180000\"") 1 1 1 1 "") 
COMMENT        #52=(CFSignal (CObList) "CMV forward primer" 27 0 0 4949 4969 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1930000\"") 1 1 1 1 "") 
COMMENT        #53=(CFSignal (CObList) "Bsd(R)" 4 0 0 1300 1698 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1050000\"") 1 1 1 1 "") 
COMMENT        #54=(CFSignal (CObList) "bla promoter" 30 0 1 4045 4143 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2330000\"") 1 1 1 1 "") 
COMMENT        #55=(CFSignal (CObList) "Amp(R)" 4 0 1 3184 4044 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1020000\"") 1 1 1 1 "") 
COMMENT        #56=(CFSignal (CObList) "SV40 late polyA (from pCS2+)" 25 0 0 8677 8872 
COMMENT             1 (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #57=(CFSignal (CObList) "EGFP" 4 0 0 7884 8600 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #58=(CFSignal (CObList) "EGFP-like chromophore" 88 0 0 8075 8088 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #59=(CFSignal (CObList) "pCS2 MCS fragment" 21 0 0 8601 8660 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #60=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #61=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pcDNA-mSUN1 EGFP" 
COMMENT                    2) 5 "" 0 4) 
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COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #63=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pcDNA-mSUN1 EGFP'" 1) 
COMMENT                     #64=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "attB1-attB2-attB5" 1) 
COMMENT                     #65=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #66=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 01/22/19 06:16AM" 1) 
COMMENT                     #67=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/24/19 03:24AM" 1) 
COMMENT                     #68=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8884 bp" 1) 
COMMENT                     #69=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Constructed" 1) 
COMMENT                     #70=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #71=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) 
COMMENT                   "Standard Fields") 
COMMENT              #72=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #73=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 11 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #74=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Mix Entry Clones pE(2F)EGFPpA (L5-L2), pE-mSUN1 L1R5 and Destination Vector pcDNA6.2/V5-DEST in LR buffer and TE buffers" 
COMMENT                          1) 
COMMENT                     #75=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Thaw, vortex and add LR Clonase enzyme (LR Clonase Plus enzyme for multi-site Gateway). Incubate >= 1 hr. at 25\260." 
COMMENT                          1) 
COMMENT                     #76=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Add Proteinase K for 10 min at 37\260." 1) 
COMMENT                     #77=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Transform into DH5a E. coli cells (One Shot TOP10 for multi-site Gateway) or any non-F' episome bearing strain." 
COMMENT                          1) 
COMMENT                     #78=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Select Expression Clones on agar plates with antibiotics ampicillin or blasticidin ." 
COMMENT                          1) 
COMMENT                     #79=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Express in appropriate host cells." 1))) 
COMMENT                   "Design Description") 
COMMENT              #80=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #81=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 51514552 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #82=(CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #13# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 86 " 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #13# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 7829 " 1))))) "AgeI: 2 sites") 1 5 
COMMENT                          "ACCGGT" "TGGCCA") 
COMMENT                     #85=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 51514168 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #86=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 2682 " 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 3925 " 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 4213 " 1))))) "ApaLI: 3 sites") 1 
COMMENT                          5 "GTGCAC" "CACGTG") 
COMMENT                     #90=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 51514584 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #91=(CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #14# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 829 " 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #14# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 1907 " 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #14# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 4308 " 1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #14# 4) 1 2 2 0 191) 
COMMENT                                        " N4: 8619 " 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #14# 5) 1 2 2 0 191) 
COMMENT                                        " N5: 8757 " 1))))) "ApoI: 5 sites") 1 5 
COMMENT                          "RAATTY" "YTTAAR") 
COMMENT                     #97=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 51514296 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "AscI: 0 sites") 2 6 "GGCGCGCC" 
COMMENT                          "CCGCGCGG") 
COMMENT                     #98=(CRSFolderPar 
COMMENT                          (CFolderPar 
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COMMENT                            (CObjectList 
COMMENT                             #99=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                         " N1: 1193 " 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                         " N2: 5463 " 1) 
COMMENT                                   #102=(CLinePar 
COMMENT                                         (CParagraph 0 (1 #4# 3) 1 2 2 0 191) 
COMMENT                                         " N3: 6261 " 1) 
COMMENT                                   #103=(CLinePar 
COMMENT                                         (CParagraph 0 (1 #4# 4) 1 2 2 0 191) 
COMMENT                                         " N4: 6531 " 1) 
COMMENT                                   #104=(CLinePar 
COMMENT                                         (CParagraph 0 (1 #4# 5) 1 2 2 0 191) 
COMMENT                                         " N5: 7096 " 1) 
COMMENT                                   #105=(CLinePar 
COMMENT                                         (CParagraph 0 (1 #4# 6) 1 2 2 0 191) 
COMMENT                                         " N6: 7472 " 1) 
COMMENT                                   #106=(CLinePar 
COMMENT                                         (CParagraph 0 (1 #4# 7) 1 2 2 0 191) 
COMMENT                                         " N7: 8632 " 1))))) "AvaI: 7 sites") 1 
COMMENT                          5 "CYCGRG" "GRGCYC") 
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COMMENT                                    (CObjectList 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 230 " 1) 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 1460 " 1) 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 3396 " 1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 3618 " 1) 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 5) 1 2 2 0 191) 
COMMENT                                           " N5: 5562 " 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 6) 1 2 2 0 191) 
COMMENT                                           " N6: 5738 " 1) 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 7) 1 2 2 0 191) 
COMMENT                                           " N7: 7443 " 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 8) 1 2 2 0 191) 
COMMENT                                           " N8: 7788 " 1) 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 9) 1 2 2 0 191) 
COMMENT                                           " N9: 8541 " 1))))) "AvaII: 9 sites") 
COMMENT                           1 4 "GGWCC" "CCWGG") 
COMMENT                     #118=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 51513912 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" 
COMMENT                           "CCTAGG") 
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COMMENT                                    (CObjectList 
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COMMENT                                           " N1: 3378 " 1) 
COMMENT                                     #122=(CLinePar 
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COMMENT                                           " N2: 4521 " 1) 
COMMENT                                     #123=(CLinePar 
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COMMENT                                           " N3: 4643 " 1) 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #17# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 4714 " 1))))) "BglI: 4 sites") 
COMMENT                           7 4 "GCCNNNNNGGC" "CGGNNNNNCCG") 
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COMMENT                                     #128=(CLinePar 
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COMMENT                                           " N2: 4193 " 1) 
COMMENT                                     #129=(CLinePar 
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COMMENT                                           " N3: 7562 " 1) 
COMMENT                                     #130=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #18# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 8611 " 1))))) "BglII: 4 sites") 
COMMENT                           1 5 "AGATCT" "TCTAGA") 
COMMENT                     #131=(CRSFolderPar 
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COMMENT                            (CGroupPar (CParagraph 51513944 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" 
COMMENT                           "TAGCTA") 
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COMMENT                                           " N1: 8619 " 1))))) "EcoRI: 1 site") 
COMMENT                           1 5 "GAATTC" "CTTAAG") 
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COMMENT                             (CObjectList)) "EcoRV: 0 sites") 3 3 "GATATC" 
COMMENT                           "CTATAG") 
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COMMENT                           1 5 "AAGCTT" "TTCGAA") 
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FEATURES             Location/Qualifiers
     misc_feature    5092..5112
                     /vntifkey="21"
                     /label=attB1
     misc_feature    complement(8877..13)
                     /vntifkey="21"
                     /label=attB2
     misc_feature    7861..7881
                     /vntifkey="21"
                     /label=attB5
     CDS             5121..7859
                     /vntifkey="4"
                     /label=mSUN1\no\stop
     CDS             91..99
                     /invitrogen="123000001"
                     /vntifkey="4"
                     /label=3\stops
     CDS             40..81
                     /invitrogen="1350000"
                     /vntifkey="4"
                     /label=V5\epitope
     primer          complement(49..69)
                     /invitrogen="2140000"
                     /vntifkey="27"
                     /label=V5\reverse\primer
     primer          complement(115..133)
                     /invitrogen="2120000"
                     /vntifkey="27"
                     /label=TK\pA\reverse\primer
     polyA_signal    108..379
                     /invitrogen="1860000"
                     /vntifkey="25"
                     /label=TK\pA
     promoter        5043..5059
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     primer          5043..5062
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     polyA_signal    1856..1986
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     promoter        848..1217
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      complement(2369..3039)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     rep_origin      415..843
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     promoter        1232..1299
                     /invitrogen="2340000"
                     /vntifkey="30"
                     /label=EM7\promoter
     promoter        4412..4999
                     /invitrogen="2180000"
                     /vntifkey="29"
                     /label=CMV\promoter
     primer          4949..4969
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     CDS             1300..1698
                     /invitrogen="1050000"
                     /vntifkey="4"
                     /label=Bsd(R)
     promoter        complement(4045..4143)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             complement(3184..4044)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     polyA_signal    8677..8872
                     /vntifkey="25"
                     /label=SV40\late\polyA\(from\pCS2+)
     CDS             7884..8600
                     /vntifkey="4"
                     /label=EGFP
     misc_structure  8075..8088
                     /vntifkey="88"
                     /label=EGFP-like\chromophore
     misc_feature    8601..8660
                     /vntifkey="21"
                     /label=pCS2\MCS\fragment
BASE COUNT     2177 a      2343 c      2276 g      2088 t 
ORIGIN
        1 ttgtacaaag tggttgatct agagggcccg cggttcgaag gtaagcctat ccctaaccct 
       61 ctcctcggtc tcgattctac gcgtaccggt tagtaatgag tttaaacggg ggaggctaac 
      121 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca 
      181 gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct 
      241 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct 
      301 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg 
      361 cggcaggccc tgccatagca gatctgcgca gctggggctc tagggggtat ccccacgcgc 
      421 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 
      481 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 
      541 ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt 
      601 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 
      661 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 
      721 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 
      781 ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 
      841 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 
      901 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 
      961 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 
     1021 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 
     1081 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 
     1141 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 
     1201 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat 
     1261 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa 
     1321 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa 
     1381 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat 
     1441 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct 
     1501 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg 
     1561 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata 
     1621 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt 
     1681 tatgtgtggg agggctaagc acttcgtggc cgaggagcag gactgacacg tgctacgaga 
     1741 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 
     1801 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 
     1861 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     1921 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 
     1981 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 
     2041 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     2101 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     2161 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
     2221 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     2281 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     2341 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     2401 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     2461 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     2521 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     2581 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
     2641 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     2701 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     2761 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     2821 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 
     2881 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     2941 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     3001 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     3061 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     3121 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
     3181 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
     3241 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
     3301 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
     3361 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
     3421 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
     3481 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
     3541 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
     3601 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
     3661 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
     3721 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
     3781 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 
     3841 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
     3901 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
     3961 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
     4021 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 
     4081 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     4141 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc 
     4201 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat 
     4261 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca 
     4321 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg 
     4381 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa 
     4441 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa 
     4501 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata 
     4561 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag 
     4621 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc 
     4681 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta 
     4741 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg 
     4801 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt 
     4861 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca 
     4921 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag 
     4981 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa 
     5041 attaatacga ctcactatag ggagacccaa gctggctagt taagctatca acaagtttgt 
     5101 acaaaaaagc aggcttaacc atggactttt ctcggctgca cacgtacacc ccaccccagt 
     5161 gtgtgccgga gaacactggc tacacttacg cactcagttc tagttactcg tcggatgctc 
     5221 tggattttga aactgagcac aagttggaac ctgtatttga ctctccaagg atgtcccgcc 
     5281 gcagcttgcg tctggtcaca acagcttcgt acagcagtgg ggacagccag gctattgatt 
     5341 cgcacattag caccagcagg gccacccccg ccaaggggag agaaaccagg acagtcaaac 
     5401 agagaagaag tgcaagcaag ccagctttta gtatcaacca cctgtcaggg aagggcttgt 
     5461 cctcgagcac aagccatgac agctcttgca gcctgcggag tgccacggtg ctgcggcacc 
     5521 ctgtgctaga tgagtccctg attcgtgagc agaccaaagt ggaccacttc tggggtctcg 
     5581 atgatgatgg tgaccttaaa ggtggaaata aagctgccac tcagggaaat ggtgaactgg 
     5641 cagcagaggt ggcgagcagc aatggataca cttgccgtga ctgcaggatg ctctcagcgc 
     5701 gcactgacgc actcacagcc cactctgcca tccacgggac cacctccagg gtgtactcca 
     5761 gagacaggac tctcaaacca cgcggagtgt ccttttacct ggataggact ctgtggctgg 
     5821 ccaagtccac ctcctcatcc tttgcatcat ttatagttca acttttccaa gtggttttaa 
     5881 tgaagctcaa ttttgaaact tacaaattga aaggctatga atccagagct tatgaatcac 
     5941 agagctatga gacaaagagc catgagtcag aagcccatct cggtcactgt gggaggatga 
     6001 ctgccggaga actttccaga gtggacgggg agtccctgtg cgatgactgt aaggggaaga 
     6061 agcaccttga gatacacaca gccacccact cgcaactgcc ccagccacac agggtggccg 
     6121 gggccatggg gcgcctctgc atctatacag gtgacctctt ggttcaagca ctgcgaagga 
     6181 ctagagctgc cgggtggtct gtggccgagg ccgtgtggtc ggtgctctgg ctggctgtct 
     6241 ctgctccagg gaaggcagcc tcgggaacct tctggtggct agggagcggc tggtaccaat 
     6301 ttgttacttt gatttcttgg ctgaatgtct ttcttcttac caggtgcctt cgaaatattt 
     6361 gcaaggtttt tgtcttgctc ctcccactcc tacttttact aggtgctggt gtctccctgt 
     6421 ggggccaggg aaacttcttc tcactcctac cagtgctgaa ctggacggcc atgcagccaa 
     6481 cacagagggt ggacgattcc aagggcatgc atagacctgg ccctcttccc ccgagcccac 
     6541 ctccaaaggt tgatcacaag gcttcccagt ggcctcagga gagtgacatg gggcagaagg 
     6601 tagcttcttt gagtgcgcag tgccacaacc atgatgagag acttgcagag ctgacagtcc 
     6661 tgcttcagaa actacagata cgggtagacc aagtggatga cggcagggaa gggctgtcac 
     6721 tgtgggtcaa gaatgtggtt ggacagcacc tgcaggagat gggcaccata gaaccacctg 
     6781 atgctaagac tgacttcatg actttccacc atgaccatga agtgcgtctc tccaacttgg 
     6841 aagatgttct tagaaaactg acagaaaaat ctgaggctat ccagaaggag ctggaagaaa 
     6901 ccaagctgaa agcaggcagc agggatgaag agcagcccct ccttgaccgt gtgcagcacc 
     6961 tagaactgga actgaacctg ttgaagtcac agctgtcaga ctggcagcat ctgaagacca 
     7021 gctgtgagca ggctggggcc cgcatccagg agactgtgca gctcatgttc tctgaggatc 
     7081 agcagggcgg ttccctcgag tggctattag agaagctttc ttctcggttc gtgagcaagg 
     7141 atgagctgca ggtgctctta catgaccttg agctgaaact gctgcagaat atcacacacc 
     7201 acatcaccgt gacaggacag gccccgacat ccgaggctat tgtgtctgcc gtgaatcagg 
     7261 cagggatttc aggaatcaca gaagcgcaag cacatatcat tgtgaacaat gctctgaagc 
     7321 tgtactccca agacaagacg gggatggtgg actttgctct ggagtctgga ggtggcagca 
     7381 tcctaagcac tcggtgctct gagacctatg agaccaagac ggcactgctg agcctgtttg 
     7441 gggtcccact gtggtacttc tcacagtcac ctcgagtggt gatccagccc gacatctacc 
     7501 cagggaattg ctgggcgttc aaaggttccc aggggtacct ggtggtgcgg ttgtccatga 
     7561 agatctaccc aaccacattc accatggaac acattccaaa gacactatca cccactggta 
     7621 acatctccag tgcccccaaa gactttgcag tctatggact ggaaacggag tatcaagaag 
     7681 aggggcagcc tctgggacgg ttcacctatg accaggaagg agactcactc cagatgttcc 
     7741 acacactgga aagacctgac caagccttcc agatagtaga gctccgggtc ctgtccaact 
     7801 ggggccaccc tgagtacact tgcctctacc ggttccgagt ccacggagag cccatccaga 
     7861 caactttgta tacaaaagtt gtgatggtga gcaagggcga ggagctgttc accggggtgg 
     7921 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 
     7981 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 
     8041 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 
     8101 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 
     8161 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 
     8221 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 
     8281 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 
     8341 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 
     8401 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 
     8461 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca 
     8521 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 
     8581 gcatggacga gctgtacaag ggcggtggaa gatctgggaa ttcaaggcct ctcgagcctc 
     8641 tagattctgc agccctatag tgagtcgtat tacgtagatc cagacatgat aagatacatt 
     8701 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt 
     8761 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac 
     8821 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt tttacccagc 
     8881 tttc 
//
