LOCUS       pcDNA-mSUN2\EGFP        8338 bp    DNA     circular     15-MAY-2018
DEFINITION  attB1-attB2-attB5.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-12289518|
COMMENT     VNTDBDATE|-12289518|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pcDNA-mSUN2 EGFP|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pcDNA-mSUN2 EGFP" 0 8338 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 121 0 "" 0 "" "" "" "" "" "" "pcDNA6.2/V5-DEST" "" 
COMMENT             0 0 1 5099 1 5099 3162 919 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 2 0 3162 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 919 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 54346744 0 "" 0 "" "" "" "" "" "" "pE-mSUN2 L1R5" 
COMMENT             "" 0 0 1 2223 5100 7322 2583 4805 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 2 0 2583 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 4805 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "pE(2F)EGFPpA (L5-L2)" 
COMMENT             "" 0 0 1 1016 7323 8338 2531 3546 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 2 0 2531 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 3546 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI" "KpnI" "NotI" "PvuI" "PvuII" "XbaI" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 8338 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #3=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 6 2682 0 3925 0 4213 0 
COMMENT            6745 0 6949 0 7294 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 8 1193 0 5372 0 5604 0 5633 
COMMENT            0 5996 0 6320 0 6926 0 8086 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 7241 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 8073 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 1079 0 1299 0 4791 0 6111 
COMMENT            0 0 "") 
COMMENT        #10=(CRSite (CStringList) "PstI" "ctgcag" 6 0 9 5879 0 6039 0 6593 0 
COMMENT             6620 0 6710 0 6773 0 6941 0 7130 0 8106 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "SmaI" "cccggg" 4 0 3 1195 0 5374 0 5998 0 0 
COMMENT             "") 
COMMENT        #12=(CRSite (CStringList) "XmaI" "cccggg" 2 0 3 1193 0 5372 0 5996 0 0 
COMMENT             "") 
COMMENT        #13=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 2 5721 0 6912 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 1536 0 3628 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "PvuII" "cagctg" 4 0 7 392 0 1508 0 2192 0 
COMMENT             5298 0 6333 0 6414 0 6615 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 19 0 8094 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 8086 0 0 "")) 
COMMENT       (CObList 
COMMENT        #19=(CFSignal (CObList) "attB1" 21 0 0 5092 5112 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #20=(CFSignal (CObList) "attB2" 21 0 1 8331 13 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "attB5" 21 0 0 7315 7335 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #22=(CFSignal (CObList) "3 stops" 4 0 0 91 99 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"123000001\"") 1 1 1 1 "") 
COMMENT        #23=(CFSignal (CObList) "V5 epitope" 4 0 0 40 81 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1350000\"") 1 1 1 1 "") 
COMMENT        #24=(CFSignal (CObList) "V5 reverse primer" 27 0 1 49 69 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2140000\"") 1 1 1 1 "") 
COMMENT        #25=(CFSignal (CObList) "TK pA reverse primer" 27 0 1 115 133 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"2120000\"") 1 1 1 1 "") 
COMMENT        #26=(CFSignal (CObList) "TK pA" 25 0 0 108 379 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1860000\"") 1 1 1 1 "") 
COMMENT        #27=(CFSignal (CObList) "T7 promoter" 30 0 0 5043 5059 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2370000\"") 1 1 1 1 "") 
COMMENT        #28=(CFSignal (CObList) "T7 primer" 27 0 0 5043 5062 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2100000\"") 1 1 1 1 "") 
COMMENT        #29=(CFSignal (CObList) "SV40 pA" 25 0 0 1856 1986 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1840000\"") 1 1 1 1 "") 
COMMENT        #30=(CFSignal (CObList) "SV40 early promoter" 29 0 0 848 1217 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"2290000\"") 1 1 1 1 "") 
COMMENT        #31=(CFSignal (CObList) "pUC origin" 33 0 1 2369 3039 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2540000\"") 1 1 1 1 "") 
COMMENT        #32=(CFSignal (CObList) "f1 origin" 33 0 0 415 843 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2500000\"") 1 1 1 1 "") 
COMMENT        #33=(CFSignal (CObList) "EM7 promoter" 30 0 0 1232 1299 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2340000\"") 1 1 1 1 "") 
COMMENT        #34=(CFSignal (CObList) "CMV promoter" 29 0 0 4412 4999 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2180000\"") 1 1 1 1 "") 
COMMENT        #35=(CFSignal (CObList) "CMV forward primer" 27 0 0 4949 4969 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1930000\"") 1 1 1 1 "") 
COMMENT        #36=(CFSignal (CObList) "Bsd(R)" 4 0 0 1300 1698 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1050000\"") 1 1 1 1 "") 
COMMENT        #37=(CFSignal (CObList) "bla promoter" 30 0 1 4045 4143 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2330000\"") 1 1 1 1 "") 
COMMENT        #38=(CFSignal (CObList) "Amp(R)" 4 0 1 3184 4044 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1020000\"") 1 1 1 1 "") 
COMMENT        #39=(CFSignal (CObList) "mSUN2 no stop" 4 0 0 5121 7313 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #40=(CFSignal (CObList) "SV40 late polyA (from pCS2+)" 25 0 0 8131 8326 
COMMENT             1 (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #41=(CFSignal (CObList) "EGFP" 4 0 0 7338 8054 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #42=(CFSignal (CObList) "EGFP-like chromophore" 88 0 0 7529 7542 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #43=(CFSignal (CObList) "pCS2 MCS fragment" 21 0 0 8055 8114 1 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #44=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #45=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pcDNA-mSUN2 EGFP" 
COMMENT                    2) 5 "" 0 4) 
COMMENT              #46=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pcDNA-mSUN2 EGFP'" 1) 
COMMENT                     #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "attB1-attB2-attB5" 1) 
COMMENT                     #49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 05/15/18 08:33AM" 1) 
COMMENT                     #51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 05/15/18 08:33AM" 1) 
COMMENT                     #52=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8338 bp" 1) 
COMMENT                     #53=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Constructed" 1) 
COMMENT                     #54=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #55=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) 
COMMENT                   "Standard Fields") 
COMMENT              #56=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #57=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 11 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #58=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Mix Entry Clones pE(2F)EGFPpA (L5-L2), pE-mSUN2 L1R5 and Destination Vector pcDNA6.2/V5-DEST in LR buffer and TE buffers" 
COMMENT                          1) 
COMMENT                     #59=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Thaw, vortex and add LR Clonase enzyme (LR Clonase Plus enzyme for multi-site Gateway). Incubate >= 1 hr. at 25\260." 
COMMENT                          1) 
COMMENT                     #60=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Add Proteinase K for 10 min at 37\260." 1) 
COMMENT                     #61=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Transform into DH5a E. coli cells (One Shot TOP10 for multi-site Gateway) or any non-F' episome bearing strain." 
COMMENT                          1) 
COMMENT                     #62=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Select Expression Clones on agar plates with antibiotics ampicillin or blasticidin ." 
COMMENT                          1) 
COMMENT                     #63=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Express in appropriate host cells." 1))) 
COMMENT                   "Design Description") 
COMMENT              #64=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #65=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 51495480 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #66=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 2682 " 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 3925 " 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 4213 " 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 4) 1 2 2 0 191) 
COMMENT                                        " N4: 6745 " 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 5) 1 2 2 0 191) 
COMMENT                                        " N5: 6949 " 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 6) 1 2 2 0 191) 
COMMENT                                        " N6: 7294 " 1))))) "ApaLI: 6 sites") 1 
COMMENT                          5 "GTGCAC" "CACGTG") 
COMMENT                     #73=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 51511032 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #74=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 1193 " 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 5372 " 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 5604 " 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 4) 1 2 2 0 191) 
COMMENT                                        " N4: 5633 " 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 5) 1 2 2 0 191) 
COMMENT                                        " N5: 5996 " 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 6) 1 2 2 0 191) 
COMMENT                                        " N6: 6320 " 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 7) 1 2 2 0 191) 
COMMENT                                        " N7: 6926 " 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 8) 1 2 2 0 191) 
COMMENT                                        " N8: 8086 " 1))))) "AvaI: 8 sites") 1 5 
COMMENT                          "CYCGRG" "GRGCYC") 
COMMENT                     #83=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 51510776 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #84=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 7241 " 1))))) "BamHI: 1 site") 1 5 
COMMENT                          "GGATCC" "CCTAGG") 
COMMENT                     #86=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 51500792 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" 
COMMENT                          "TAGCTA") 
COMMENT                     #87=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 51497560 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #88=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #7# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 8073 " 1))))) "EcoRI: 1 site") 1 5 
COMMENT                          "GAATTC" "CTTAAG") 
COMMENT                     #90=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 51505208 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" 
COMMENT                          "TTCGAA") 
COMMENT                     #91=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 51504152 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #92=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #13# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #13# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 5721 " 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #13# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 6912 " 1))))) "KpnI: 2 sites") 5 1 
COMMENT                          "GGTACC" "CCATGG") 
COMMENT                     #95=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 51501688 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #96=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #9# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #9# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 1079 " 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #9# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 1299 " 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #9# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 4791 " 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (1 #9# 4) 1 2 2 0 191) 
COMMENT                                         " N4: 6111 " 1))))) "NcoI: 4 sites") 1 
COMMENT                          5 "CCATGG" "GGTACC") 
COMMENT                     #101=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 51500536 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList)) "NotI: 0 sites") 2 6 "GCGGCCGC" 
COMMENT                           "CGCCGGCG") 
COMMENT                     #102=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 51501880 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #103=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #10# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #104=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #10# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 5879 " 1) 
COMMENT                                     #105=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #10# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 6039 " 1) 
COMMENT                                     #106=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #10# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 6593 " 1) 
COMMENT                                     #107=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #10# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 6620 " 1) 
COMMENT                                     #108=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #10# 5) 1 2 2 0 191) 
COMMENT                                           " N5: 6710 " 1) 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #10# 6) 1 2 2 0 191) 
COMMENT                                           " N6: 6773 " 1) 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #10# 7) 1 2 2 0 191) 
COMMENT                                           " N7: 6941 " 1) 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #10# 8) 1 2 2 0 191) 
COMMENT                                           " N8: 7130 " 1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #10# 9) 1 2 2 0 191) 
COMMENT                                           " N9: 8106 " 1))))) "PstI: 9 sites") 
COMMENT                           5 1 "CTGCAG" "GACGTC") 
COMMENT                     #113=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 51499448 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #114=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #15# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #15# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 1536 " 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #15# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 3628 " 1))))) "PvuI: 2 sites") 
COMMENT                           4 2 "CGATCG" "GCTAGC") 
COMMENT                     #117=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 51503032 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #118=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #16# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 392 " 1) 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 1508 " 1) 
COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 2192 " 1) 
COMMENT                                     #122=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 5298 " 1) 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 5) 1 2 2 0 191) 
COMMENT                                           " N5: 6333 " 1) 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 6) 1 2 2 0 191) 
COMMENT                                           " N6: 6414 " 1) 
COMMENT                                     #125=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #16# 7) 1 2 2 0 191) 
COMMENT                                           " N7: 6615 " 1))))) "PvuII: 7 sites") 
COMMENT                           3 3 "CAGCTG" "GTCGAC") 
COMMENT                     #126=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 51502200 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #127=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #11# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #128=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #11# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 1195 " 1) 
COMMENT                                     #129=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #11# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 5374 " 1) 
COMMENT                                     #130=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #11# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 5998 " 1))))) "SmaI: 3 sites") 
COMMENT                           3 3 "CCCGGG" "GGGCCC") 
COMMENT                     #131=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 51503224 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #132=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #17# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #133=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #17# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 19 " 1) 
COMMENT                                     #134=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #17# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 8094 " 1))))) "XbaI: 2 sites") 
COMMENT                           1 5 "TCTAGA" "AGATCT") 
COMMENT                     #135=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 51862600 (8 0) 1 1 1 0 178) 
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FEATURES             Location/Qualifiers
     misc_feature    5092..5112
                     /vntifkey="21"
                     /label=attB1
     misc_feature    complement(8331..13)
                     /vntifkey="21"
                     /label=attB2
     misc_feature    7315..7335
                     /vntifkey="21"
                     /label=attB5
     CDS             91..99
                     /invitrogen="123000001"
                     /vntifkey="4"
                     /label=3\stops
     CDS             40..81
                     /invitrogen="1350000"
                     /vntifkey="4"
                     /label=V5\epitope
     primer          complement(49..69)
                     /invitrogen="2140000"
                     /vntifkey="27"
                     /label=V5\reverse\primer
     primer          complement(115..133)
                     /invitrogen="2120000"
                     /vntifkey="27"
                     /label=TK\pA\reverse\primer
     polyA_signal    108..379
                     /invitrogen="1860000"
                     /vntifkey="25"
                     /label=TK\pA
     promoter        5043..5059
                     /invitrogen="2370000"
                     /vntifkey="30"
                     /label=T7\promoter
     primer          5043..5062
                     /invitrogen="2100000"
                     /vntifkey="27"
                     /label=T7\primer
     polyA_signal    1856..1986
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     promoter        848..1217
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      complement(2369..3039)
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     rep_origin      415..843
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=f1\origin
     promoter        1232..1299
                     /invitrogen="2340000"
                     /vntifkey="30"
                     /label=EM7\promoter
     promoter        4412..4999
                     /invitrogen="2180000"
                     /vntifkey="29"
                     /label=CMV\promoter
     primer          4949..4969
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     CDS             1300..1698
                     /invitrogen="1050000"
                     /vntifkey="4"
                     /label=Bsd(R)
     promoter        complement(4045..4143)
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             complement(3184..4044)
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     CDS             5121..7313
                     /vntifkey="4"
                     /label=mSUN2\no\stop
     polyA_signal    8131..8326
                     /vntifkey="25"
                     /label=SV40\late\polyA\(from\pCS2+)
     CDS             7338..8054
                     /vntifkey="4"
                     /label=EGFP
     misc_structure  7529..7542
                     /vntifkey="88"
                     /label=EGFP-like\chromophore
     misc_feature    8055..8114
                     /vntifkey="21"
                     /label=pCS2\MCS\fragment
BASE COUNT     1975 a      2252 c      2183 g      1928 t 
ORIGIN
        1 ttgtacaaag tggttgatct agagggcccg cggttcgaag gtaagcctat ccctaaccct 
       61 ctcctcggtc tcgattctac gcgtaccggt tagtaatgag tttaaacggg ggaggctaac 
      121 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca 
      181 gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct 
      241 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct 
      301 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg 
      361 cggcaggccc tgccatagca gatctgcgca gctggggctc tagggggtat ccccacgcgc 
      421 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 
      481 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 
      541 ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt 
      601 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 
      661 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 
      721 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 
      781 ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 
      841 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 
      901 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 
      961 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 
     1021 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 
     1081 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 
     1141 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 
     1201 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat 
     1261 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa 
     1321 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa 
     1381 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat 
     1441 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct 
     1501 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg 
     1561 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata 
     1621 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt 
     1681 tatgtgtggg agggctaagc acttcgtggc cgaggagcag gactgacacg tgctacgaga 
     1741 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 
     1801 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 
     1861 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     1921 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 
     1981 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 
     2041 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     2101 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     2161 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
     2221 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     2281 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     2341 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     2401 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     2461 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     2521 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     2581 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 
     2641 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     2701 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     2761 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     2821 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 
     2881 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     2941 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     3001 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     3061 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     3121 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
     3181 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
     3241 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
     3301 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
     3361 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
     3421 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
     3481 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
     3541 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
     3601 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
     3661 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
     3721 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
     3781 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 
     3841 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
     3901 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
     3961 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
     4021 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 
     4081 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     4141 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc 
     4201 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat 
     4261 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca 
     4321 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg 
     4381 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa 
     4441 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa 
     4501 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata 
     4561 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag 
     4621 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc 
     4681 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta 
     4741 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg 
     4801 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt 
     4861 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca 
     4921 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag 
     4981 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa 
     5041 attaatacga ctcactatag ggagacccaa gctggctagt taagctatca acaagtttgt 
     5101 acaaaaaagc aggcttaacc atgtcgagac gaagccagcg cctcactcgc tactctcagg 
     5161 atgataacga tggcggcagc agcagcagtg gtgcgagctc cgtggcagga agccagggca 
     5221 ccgtgtttaa agacagtcct ctcaggactt tgaagaggaa atccagcaac atgaagcacc 
     5281 tgtccccagc tccacagctg ggcccctcct ctgactccca cacctcctac tacagcgagt 
     5341 ctgtggttcg agagtcctac atcggcagcc cccgggctgt gtccctcgcc aggagtgccc 
     5401 tcctggatga ccacctacac agtgagccct actggagcgg ggaccttcgg gggaggagga 
     5461 ggagaggaac aggtggttct gagagcagca aggccaatgg gctcaccgcg gagagcaagg 
     5521 cctcagaaga ctttttcgga tcttcctcag gctattcttc agaggatgac cttgcaggct 
     5581 acacggactc agaccagcac agctcggggt ccaggttaag gagtgcagca tctcgggccg 
     5641 gctcctttgt ctggactctg gtcacttttc caggccgcct ctttggtctt ctctactggt 
     5701 ggattggcac cacctggtac cgcctgacaa ctgctgcctc cctcctggat gtcttcgtcc 
     5761 taaccaggtc caggcacttc tcgctgaacc tgaagagttt tctgtggttc cttctgctct 
     5821 tgctactcct gactggtctg acctacggtg cttggcattt ttacccctta gggctgcaga 
     5881 cattgcaacc cgctgtggtc tcctggtggg cagcaaaaga gagcaggaag cagccagagg 
     5941 tgtgggaatc cagagacgcc tcccagcact tccaggctga gcagcgcgtt ctctcccggg 
     6001 ttcactctct ggagcggcgt ctggaagccc ttgctgcaga cttttcctcc aactggcaga 
     6061 aggaggccat acggctggaa cgcctggagc tgcggcaggg ggctgctggc catggaggag 
     6121 gcagtagcct gagccatgaa gatgccctgt ctctcctaga agggttggtg agccgccgcg 
     6181 aggctaccct gaaggaggac ttgcgcaggg acacagtggc tcatatccag gaagaattgg 
     6241 ctaccctgag ggcagagcat caccaagact cggaagatct cttcaagaag atcgtccagg 
     6301 cctctcagga gtccgaagcc cgagtccagc agctgaagac agaatggaaa agcatgaccc 
     6361 aggaggcctt ccaggagagc tctgtgaagg agctgggacg gctggaagcc cagctggcca 
     6421 gcctgcggca ggagctggct gccctgactc tgaagcagaa ctcggtggca gatgaagtgg 
     6481 gcctgctgcc acagaagatc caggctgcca gggctgatgt ggaatcccag ttccctgact 
     6541 ggatcaggca gttccttctt ggagacaggg gtgcgcgcag cgggctcctg cagagagatg 
     6601 agatgcacgc tcagctgcag gagctggaga acaagatcct taccaagatg gctgagatgc 
     6661 agggcaagtc agccagggag gccgcagcgt ccctgggaca gatactgcag aaagaaggca 
     6721 tagttggggt gacagaggag caggtgcacc ggatcgtcaa gcaggccctg cagcgctaca 
     6781 gtgaggacag gattggaatg gtggattacg ccctggaatc aggaggagcc agtgttatca 
     6841 gcacccgctg ctctgagact tatgagacca agacggcact cctcagcctc tttggcatcc 
     6901 ccctgtggta ccactcccag tcacctcggg tcattctgca gccagatgtg cacccaggca 
     6961 actgctgggc cttccagggg ccccagggct ttgcagtggt ccgcctctct gctcgaatcc 
     7021 gacctacagc cgttacctta gagcacgtgc ccaaggcctt gtcacccaac agcactatct 
     7081 ccagtgctcc caaggacttc gccatctttg gcttcgatga agacctgcag caggaaggga 
     7141 cacttcttgg cacgtttgcc tacgaccagg atggggagcc catccagacc ttctatttcc 
     7201 aggcctctaa gatggccaca taccaagttg tggagcttcg gatcctgacc aactggggcc 
     7261 accctgagta cacgtgtatc taccgcttcc gggtgcacgg agagcctgcc cacacaactt 
     7321 tgtatacaaa agttgtgatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 
     7381 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 
     7441 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 
     7501 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct 
     7561 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 
     7621 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 
     7681 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 
     7741 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg 
     7801 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg 
     7861 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 
     7921 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga 
     7981 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 
     8041 acgagctgta caagggcggt ggaagatctg ggaattcaag gcctctcgag cctctagatt 
     8101 ctgcagccct atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag 
     8161 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat 
     8221 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc 
     8281 attcatttta tgtttcaggt tcagggggag gtgtgggagg tttttttacc cagctttc 
//
