LOCUS       pCMV-mPom121\GFP        8040 bp    DNA     circular SYN 19-JUL-2019
DEFINITION  Mammalian expression vector for expression of GFP (CMV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-GFP.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Electroporation and RNA interference in the rodent retina in vivo 
            and in vitro.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12904349|
COMMENT     VNTDBDATE|-12908270|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCMV-mPom121 GFP|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pCMV-mPom121 GFP'" 1) 
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COMMENT                          "Mammalian expression vector for expression of GFP (CMV promoter)." 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
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COMMENT                          "  TITLE     Electroporation and RNA interference in the rodent retina in vivo " 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1272..1293" 1))) "EGFP-C") 
COMMENT                             #138=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4986 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #139=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4986  End: 5005 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #140=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Rabbit beta-globin polyA region, reverse primer" 
COMMENT                                           1) 
COMMENT                                     #141=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #142=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(1425..1444)" 1))) 
COMMENT                                   "Bglob-pA-R") 
COMMENT                             #143=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5105 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #144=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5105  End: 5124 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #145=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" 
COMMENT                                           1) 
COMMENT                                     #146=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #147=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(1544..1563)" 1))) 
COMMENT                                   "rbglobpA-R") 
COMMENT                             #148=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5467 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #149=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5467  End: 5483 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #150=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "common sequencing primer, one of multiple similar variants" 
COMMENT                                           1) 
COMMENT                                     #151=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #152=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(1906..1922)" 1))) 
COMMENT                                   "M13 rev") 
COMMENT                             #153=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5467 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #154=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5467  End: 5483 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #155=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "In lacZ gene. Also called M13-rev" 1) 
COMMENT                                     #156=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #157=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(1906..1922)" 1))) 
COMMENT                                   "M13 Reverse") 
COMMENT                             #158=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5480 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #159=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5480  End: 5502 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #160=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "In lacZ gene" 1) 
COMMENT                                     #161=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #162=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(1919..1941)" 1))) 
COMMENT                                   "M13/pUC Reverse") 
COMMENT                             #163=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5748 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #164=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5748  End: 5767" 1) 
COMMENT                                     #165=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "SV40 promoter/origin, forward primer" 
COMMENT                                           1) 
COMMENT                                     #166=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #167=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2187..2206" 1))) "SV40pro-F") 
COMMENT                             #168=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5878 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #169=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5878  End: 5897 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #170=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "SV40 polyA, reverse primer" 1) 
COMMENT                                     #171=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #172=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(2317..2336)" 1))) 
COMMENT                                   "SV40pA-R") 
COMMENT                             #173=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5932 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #174=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5932  End: 5951" 1) 
COMMENT                                     #175=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "SV40 polyA terminator, reverse primer" 
COMMENT                                           1) 
COMMENT                                     #176=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #177=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2371..2390" 1))) "EBV-rev") 
COMMENT                             #178=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 6043 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #179=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 6043  End: 6060 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #180=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "L4440 vector, forward primer" 1) 
COMMENT                                     #181=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #182=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(2482..2499)" 1))) 
COMMENT                                   "L4440") 
COMMENT                             #183=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 6294 (3 #25# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #184=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 6294  End: 6313 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #185=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "pBR322 origin, forward primer" 1) 
COMMENT                                     #186=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #187=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(2733..2752)" 1))) 
COMMENT                                   "pBR322ori-F") 
COMMENT                             #188=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 7596 (3 #27# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #189=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7596  End: 7615" 1) 
COMMENT                                     #190=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Ampicillin resistance gene, reverse primer" 
COMMENT                                           1) 
COMMENT                                     #191=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #192=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4035..4054" 1))) "Amp-R"))) 
COMMENT                           "Primer Binding Site (15 total)") 
COMMENT                     #193=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #194=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 305 (3 #1# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #195=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 305   End: 508 " 1) 
COMMENT                                     #196=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "human cytomegalovirus (CMV) immediate early promoter" 
COMMENT                                           1) 
COMMENT                                     #197=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #198=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   305..508" 1))) "CMV promoter") 
COMMENT                             #199=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5515 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #200=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5515  End: 5545 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #201=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "promoter for the E. coli lac operon" 
COMMENT                                           1) 
COMMENT                                     #202=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #203=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(1954..1984)" 1))) 
COMMENT                                   "lac promoter") 
COMMENT                             #204=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5640 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #205=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5640  End: 5835" 1) 
COMMENT                                     #206=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "SV40 early promoter" 1) 
COMMENT                                     #207=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #208=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2079..2274" 1))) "SV40 promoter") 
COMMENT                             #209=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 7834 (3 #28# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #210=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7834  End: 7938 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #211=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #212=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(4273..4377)" 1) 
COMMENT                                     #213=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #214=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /gene=\"bla\"" 1))) 
COMMENT                                   "AmpR promoter"))) 
COMMENT                           "Promoter Prokaryotic (4 total)") 
COMMENT                     #215=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 31 (7 31 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #216=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5491 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #217=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5491  End: 5507" 1) 
COMMENT                                     #218=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." 
COMMENT                                           1) 
COMMENT                                     #219=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #220=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1930..1946" 1) 
COMMENT                                     #221=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #222=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /bound_moiety=\"lac repressor encoded by lacI\"" 
COMMENT                                           1))) "lac operator") 
COMMENT                             #223=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5560 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #224=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5560  End: 5581" 1) 
COMMENT                                     #225=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "CAP binding activates transcription in the presence of cAMP." 
COMMENT                                           1) 
COMMENT                                     #226=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #227=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1999..2020" 1) 
COMMENT                                     #228=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #229=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /bound_moiety=\"E. coli catabolite activator protein\"" 
COMMENT                                           1))) "CAP binding site"))) 
COMMENT                           "Protein Binding Site (2 total)") 
COMMENT                     #230=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #231=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5686 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #232=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5686  End: 5821" 1) 
COMMENT                                     #233=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "SV40 origin of replication" 1) 
COMMENT                                     #234=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #235=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2125..2260" 1))) "SV40 ori") 
COMMENT                             #236=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 6214 (3 #24# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #237=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 6214  End: 6802 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #238=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" 
COMMENT                                           1) 
COMMENT                                     #239=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #240=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(2653..3241)" 1) 
COMMENT                                     #241=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #242=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /direction=LEFT" 1))) "ori"))) 
COMMENT                           "Replication Origin (2 total)"))) "Feature Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #243=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #244=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #245=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #246=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #247=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #248=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #249=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #250=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #251=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #252=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #253=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #254=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #255=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #256=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #257=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #258=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #259=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #260=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #261=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #262=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #263=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #264=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #265=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #266=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #267=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #268=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #269=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #270=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #271=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #272=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #273=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #274=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #275=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #276=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #277=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #278=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #279=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #280=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
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FEATURES             Location/Qualifiers
     enhancer        1..304
                     /vntifkey="9"
                     /label=CMV\enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /vntifkey="30"
                     /label=CMV\promoter
                     /note="human cytomegalovirus (CMV) immediate early promoter"
     primer_bind     459..479
                     /vntifkey="28"
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4174..4183
                     /regulatory_class="other"
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="Unknown feature type:regulatory  vertebrate consensus sequence for strong initiation  of translation (Kozak, 1987)"
     CDS             4180..4899
                     /codon_start=1
                     /product="enhanced GFP"
                     /vntifkey="4"
                     /label=EGFP
                     /note="mammalian codon-optimized"
     primer_bind     complement(4225..4246)
                     /vntifkey="28"
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4486..4505)
                     /vntifkey="28"
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4833..4854
                     /vntifkey="28"
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4986..5005)
                     /vntifkey="28"
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_site      5051..5106
                     /vntifkey="26"
                     /label=-beta-globin\poly(A)\signal
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(5105..5124)
                     /vntifkey="28"
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R"
     primer_bind     complement(5467..5483)
                     /vntifkey="28"
                     /label=M13\rev
                     /note="common sequencing primer, one of multiple similar variants"
     primer_bind     complement(5467..5483)
                     /vntifkey="28"
                     /label=M13\Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5480..5502)
                     /vntifkey="28"
                     /label=M13/pUC\Reverse
                     /note="In lacZ gene"
     protein_bind    5491..5507
                     /bound_moiety="lac repressor encoded by lacI"
                     /vntifkey="31"
                     /label=lac\operator
                     /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5515..5545)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5560..5581
                     /bound_moiety="E. coli catabolite activator protein"
                     /vntifkey="31"
                     /label=CAP\binding\site
                     /note="CAP binding activates transcription in the presence of cAMP."
     promoter        5640..5835
                     /vntifkey="30"
                     /label=SV40\promoter
                     /note="SV40 early promoter"
     rep_origin      5686..5821
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="SV40 origin of replication"
     primer_bind     5748..5767
                     /vntifkey="28"
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    5841..5975
                     /vntifkey="25"
                     /label=SV40\poly(A)\signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5878..5897)
                     /vntifkey="28"
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5932..5951
                     /vntifkey="28"
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6043..6060)
                     /vntifkey="28"
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6214..6802)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     complement(6294..6313)
                     /vntifkey="28"
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6973..7833)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     primer_bind     7596..7615
                     /vntifkey="28"
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7834..7938)
                     /gene="bla"
                     /vntifkey="30"
                     /label=AmpR\promoter
     misc_feature    4165..4166
                     /vntifkey="21"
                     /label=+2bp
     CDS             565..4164
                     /vntifkey="4"
                     /label=mPom121\no\stop
     misc_feature    559..564
                     /vntifkey="21"
                     /label=Kozak
BASE COUNT     1793 a      2492 c      1959 g      1796 t 
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      541 ccggactcag atctcgaggc caccatgtct ccggcggctg cggcggctga cggaggcgag 
      601 cggcggcggc ccccgttggg cggacgggaa ggccggagcc gggcccgcgg ctacggagga 
      661 ccggcgggtg ctgcggccct cggcctagcg ctgctcggcc tcgcgctcta cctggtgccc 
      721 gcggcggccg cgctggcctg gctggccgtg ggggcgagcg cggcctggtg gggcctgagc 
      781 cgagaacccc gcggcccgag ggccctgtcg tccttcgtgc gcgacgcgcg ccgtcacccg 
      841 cggccagctc tcaccgcttc accgcctccg gccaagtcac cagtcaacgg tagcctctgc 
      901 gaaccccgca gcccgctggg aggccccgac ccggctgagc tcttactcat gggcagctac 
      961 ctgggcaagc ccggcccgcc cgagcctgcc ctccggcagg acccaaggga gaggccgggg 
     1021 cgccgcccac ccgcacgctc cccgcctccg gcctcggcgg tccagcgagt ccaccacgtc 
     1081 taccccgcgc tccccacccc gcttttgcga ccctcccgga ggccgcccca ccgagattgt 
     1141 gggcctttgt ccagtcggtt tgttataaca ccccgaagac gatatcccat tcagcaagcc 
     1201 cagtattcct tgcttggggc acttccaacc gtctgctgga atggtggcca taagaaggct 
     1261 gtgctgtctc cacggaactc caggatggtg tgcagcccag tgacagtgag gattgcccct 
     1321 ccagacagca agctattccg ctcatcaatg tcagagcaga tactcgatac aacactgtct 
     1381 tcaccatcaa gtaatgcccc agatccttgt gcaaaggaga ctgtcctgaa cgccctcaag 
     1441 gagaagaaga aaagaacagt ggcggaggaa gaccagctgc accttgatgg ccaggaaaac 
     1501 aagagaaggc gccatgatag cggtgggagc ggacattcag cattcgagcc ccttgtggcc 
     1561 aacggagtcc ctgctgcatt tgtgcctaag cctggctccc tgaagagaag tctggcttcc 
     1621 cagagctcag atgaccactt gaacaaacgg tctcgcacct cctctgtgag ctccctggct 
     1681 agtgcgtgca caggaggcat ccccagttct agccgcaatg ccatcaccag ctcctacagc 
     1741 tccacccgtg gcatctcaca gctgtggaag aggagtggtc ccacatcttc tcccttctcc 
     1801 agtccagctt cctcccgctc ccagacacca gagaggccag ccaagaagac aagagaggag 
     1861 gagccatgtc agcagtccag ttcttcacct cccttggtga cagacaagga gtccccagga 
     1921 gaaaaggtta cagatacaac cactgggaag cagcagagct catggacttc cccacccaca 
     1981 cctggcagct ccgggcaacg caaacgcaag atccagctgc tgccctcccg gcgaggggac 
     2041 cagctcacct tgcctccacc cccagagctt ggctattcca tcactgctga agacctggac 
     2101 atggagagga aagcctcact gcaatggttt aacaaggtct tggaggacaa gccagatgat 
     2161 gcttccgcct cagccactga tggcccccct tccaccagcc ctcccttcac attcaccctg 
     2221 cccgctgtgg ggcctgctgc ctcgccagcc tccctcccgg ctcccagctc taacccactg 
     2281 ttggagagct tgaagaagat gcaggagtct ccagccccat catcctcgga acctgctgag 
     2341 gcagcaacag ttgcagcccc ttcacctccg aagacaccca gcctcttggc ccctcttgtc 
     2401 tcaccactgg cagggcccct agccagcacc tcctcagact ccaaacctgc agccaccttc 
     2461 ctggggctgg cttctgcttc gtctataacc ccactcactg acagcaagtc ctctggagtc 
     2521 tctcaggctg agcagtctgt cagcacccca gcctccacag cctcctcccc gaccccaaag 
     2581 cccagcatgc tgtttgggat gctgagtcca cctgccagct cttcctccct tgccactcca 
     2641 gctccggctt gtgcttctcc catgtttaag cccatttttc cagccacacc taaaagtgag 
     2701 agtgatagcc ccttaccaag tagctcctca gccgccacta cagcttcatc cagcaccgcc 
     2761 cctcccacag cagccagcac cacacccacg ttcaagccca tttttgacaa gatggagcca 
     2821 ttcacagcca tgcccctgtc aactcccttc tccctgaagc agacaactgc tacagccacc 
     2881 actacagcca catcagctcc tctctttact ggcctgggca ctgccacttc cacggtggcc 
     2941 tctggcaccg cagcttctgc ctcaaagcct gtctttggct ttggagtgac cactgcagcg 
     3001 agtactgcca gcagtaccat gacttccact tcccagtcag ttctgttcgg gggagccccc 
     3061 cctgttacta cttctagctc tgccccagct ctggcctcca tcttccagtt tggcaagcct 
     3121 cttgccccag cagcatctgc agcaggcacc tcctttagcc agccccttgc cagctctacc 
     3181 cagactgctg ccagcaatag tggcttcagt ggttttggca gcaccctcac cacctccacc 
     3241 tcggcccctg ccaccaccag tcagcccacg ctgaccttca gcaacactgt cacccccacc 
     3301 ttcaacattc cctttagctc cagtgccaag cctgcgctcc caacctaccc tggagccaac 
     3361 tcacagccca catttggagc caccgatggg gccaccaagc cagcacttgc cccaagtttt 
     3421 ggcagctcgt tcactttcgg caactctgtg gcctcagccc catcggcagc cccagcacca 
     3481 gccacctttg gcagtgctgc acagccagct ttcggagggt tgaaagccgc agcctccacc 
     3541 ttcggcgccc ctgccagcac tcagccagct tttggtagca ccacgtctgt gttctccttt 
     3601 gggtcagcta ccacatctgg ctttggtgct gctgccaccg ctgctaccac cacacagacc 
     3661 accaacagtg ggagcagcag ctctctgttt ggcagctctg ctccatcccc attcacattc 
     3721 ggtggctcag cagctcctgc tggcagtgga ggctttggcc ttagtgctac cccaggcacc 
     3781 agctccacct ctggaacttt cagctttgga tctggacaga gtgggacccc gggcaccacc 
     3841 acctcctttg gaagtctgag tcagaacacc ctgggcgcac ccagccaggg ctcacccttt 
     3901 gccttcagtg tgggcagtac acctgaaagc aagcctgtgt ttggaggcac atctacacct 
     3961 actttcgggc agagtgcccc tgcccccggc gtaggcacca cagggagcag cctctcattt 
     4021 ggggcctctt caacacctgc ccaaggcttt gttggagttg gaccttttgg atcagcagct 
     4081 ccttcgtttt ccattggtgc gggatccaag accccagggg ctcgacagcg acttcaggcc 
     4141 cgaaggcagc acacccgcaa gaagccaccg gtcgccacca tggtgagcaa gggcgaggag 
     4201 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 
     4261 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 
     4321 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac 
     4381 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 
     4441 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 
     4501 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 
     4561 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 
     4621 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag 
     4681 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 
     4741 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc 
     4801 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 
     4861 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcac tcctcaggtg 
     4921 caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac 
     4981 tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg 
     5041 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt 
     5101 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg 
     5161 gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa 
     5221 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc 
     5281 ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc 
     5341 ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca 
     5401 gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta 
     5461 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 
     5521 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 
     5581 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg 
     5641 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 
     5701 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 
     5761 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 
     5821 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca 
     5881 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 
     5941 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg 
     6001 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 
     6061 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     6121 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
     6181 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
     6241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
     6301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
     6361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 
     6421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
     6481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
     6541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
     6601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 
     6661 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
     6721 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
     6781 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
     6841 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
     6901 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
     6961 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
     7021 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
     7081 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
     7141 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
     7201 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
     7261 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 
     7321 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
     7381 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
     7441 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
     7501 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
     7561 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 
     7621 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
     7681 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
     7741 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
     7801 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
     7861 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     7921 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat 
     7981 tgactagtaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
//
