LOCUS       p-mPom121\mScarl        8273 bp    DNA     circular SYN 19-JUL-2019
DEFINITION  In vivo visualization of actin cytoskeleton (can be used for colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    pLifeAct_mScarlet_N1.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12904345|
COMMENT     VNTDBDATE|-12908270|
COMMENT     LSOWNER|
COMMENT     VNTNAME|p-mPom121 mScarlet|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                                     #182=(CLinePar 
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COMMENT                                           "human cytomegalovirus (CMV) immediate early promoter" 
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FEATURES             Location/Qualifiers
     enhancer        61..364
                     /vntifkey="9"
                     /label=CMV\enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /vntifkey="30"
                     /label=CMV\promoter
                     /note="human cytomegalovirus (CMV) immediate early promoter"
     primer_bind     519..539
                     /vntifkey="28"
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4234..4243
                     /regulatory_class="other"
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="Unknown feature type:regulatory  vertebrate consensus sequence for strong initiation  of translation (Kozak, 1987)"
     CDS             4240..4935
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved
                     from a synthetic template (Bindels et al., 2016)"
                     /vntifkey="4"
                     /label=mScarlet
     primer_bind     complement(4373..4391)
                     /vntifkey="28"
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4641..4660
                     /vntifkey="28"
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5061..5182
                     /vntifkey="25"
                     /label=SV40\poly(A)\signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5098..5117)
                     /vntifkey="28"
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5152..5171
                     /vntifkey="28"
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5189..5644)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=f1\ori
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     primer_bind     complement(5326..5347)
                     /vntifkey="28"
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5538..5557
                     /vntifkey="28"
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5671..5775
                     /gene="bla"
                     /vntifkey="30"
                     /label=AmpR\promoter
     promoter        5777..6134
                     /vntifkey="30"
                     /label=SV40\promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(5800..5820)
                     /vntifkey="28"
                     /label=pBABE\3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5985..6120
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="SV40 origin of replication"
     primer_bind     6047..6066
                     /vntifkey="28"
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6169..6963
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /vntifkey="4"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))"
     primer_bind     complement(6223..6242)
                     /vntifkey="28"
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6833..6852
                     /vntifkey="28"
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7151..7170)
                     /vntifkey="28"
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7195..7242
                     /vntifkey="25"
                     /label=HSV\TK\poly(A)\signal
                     /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7571..8159
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     8060..8079
                     /vntifkey="28"
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4225..4226
                     /vntifkey="21"
                     /label=+2bp
     CDS             625..4224
                     /vntifkey="4"
                     /label=mPom121\no\stop
     misc_feature    619..624
                     /vntifkey="21"
                     /label=Kozak
BASE COUNT     1812 a      2576 c      2144 g      1741 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgaggc caccatgtct ccggcggctg cggcggctga cggaggcgag 
      661 cggcggcggc ccccgttggg cggacgggaa ggccggagcc gggcccgcgg ctacggagga 
      721 ccggcgggtg ctgcggccct cggcctagcg ctgctcggcc tcgcgctcta cctggtgccc 
      781 gcggcggccg cgctggcctg gctggccgtg ggggcgagcg cggcctggtg gggcctgagc 
      841 cgagaacccc gcggcccgag ggccctgtcg tccttcgtgc gcgacgcgcg ccgtcacccg 
      901 cggccagctc tcaccgcttc accgcctccg gccaagtcac cagtcaacgg tagcctctgc 
      961 gaaccccgca gcccgctggg aggccccgac ccggctgagc tcttactcat gggcagctac 
     1021 ctgggcaagc ccggcccgcc cgagcctgcc ctccggcagg acccaaggga gaggccgggg 
     1081 cgccgcccac ccgcacgctc cccgcctccg gcctcggcgg tccagcgagt ccaccacgtc 
     1141 taccccgcgc tccccacccc gcttttgcga ccctcccgga ggccgcccca ccgagattgt 
     1201 gggcctttgt ccagtcggtt tgttataaca ccccgaagac gatatcccat tcagcaagcc 
     1261 cagtattcct tgcttggggc acttccaacc gtctgctgga atggtggcca taagaaggct 
     1321 gtgctgtctc cacggaactc caggatggtg tgcagcccag tgacagtgag gattgcccct 
     1381 ccagacagca agctattccg ctcatcaatg tcagagcaga tactcgatac aacactgtct 
     1441 tcaccatcaa gtaatgcccc agatccttgt gcaaaggaga ctgtcctgaa cgccctcaag 
     1501 gagaagaaga aaagaacagt ggcggaggaa gaccagctgc accttgatgg ccaggaaaac 
     1561 aagagaaggc gccatgatag cggtgggagc ggacattcag cattcgagcc ccttgtggcc 
     1621 aacggagtcc ctgctgcatt tgtgcctaag cctggctccc tgaagagaag tctggcttcc 
     1681 cagagctcag atgaccactt gaacaaacgg tctcgcacct cctctgtgag ctccctggct 
     1741 agtgcgtgca caggaggcat ccccagttct agccgcaatg ccatcaccag ctcctacagc 
     1801 tccacccgtg gcatctcaca gctgtggaag aggagtggtc ccacatcttc tcccttctcc 
     1861 agtccagctt cctcccgctc ccagacacca gagaggccag ccaagaagac aagagaggag 
     1921 gagccatgtc agcagtccag ttcttcacct cccttggtga cagacaagga gtccccagga 
     1981 gaaaaggtta cagatacaac cactgggaag cagcagagct catggacttc cccacccaca 
     2041 cctggcagct ccgggcaacg caaacgcaag atccagctgc tgccctcccg gcgaggggac 
     2101 cagctcacct tgcctccacc cccagagctt ggctattcca tcactgctga agacctggac 
     2161 atggagagga aagcctcact gcaatggttt aacaaggtct tggaggacaa gccagatgat 
     2221 gcttccgcct cagccactga tggcccccct tccaccagcc ctcccttcac attcaccctg 
     2281 cccgctgtgg ggcctgctgc ctcgccagcc tccctcccgg ctcccagctc taacccactg 
     2341 ttggagagct tgaagaagat gcaggagtct ccagccccat catcctcgga acctgctgag 
     2401 gcagcaacag ttgcagcccc ttcacctccg aagacaccca gcctcttggc ccctcttgtc 
     2461 tcaccactgg cagggcccct agccagcacc tcctcagact ccaaacctgc agccaccttc 
     2521 ctggggctgg cttctgcttc gtctataacc ccactcactg acagcaagtc ctctggagtc 
     2581 tctcaggctg agcagtctgt cagcacccca gcctccacag cctcctcccc gaccccaaag 
     2641 cccagcatgc tgtttgggat gctgagtcca cctgccagct cttcctccct tgccactcca 
     2701 gctccggctt gtgcttctcc catgtttaag cccatttttc cagccacacc taaaagtgag 
     2761 agtgatagcc ccttaccaag tagctcctca gccgccacta cagcttcatc cagcaccgcc 
     2821 cctcccacag cagccagcac cacacccacg ttcaagccca tttttgacaa gatggagcca 
     2881 ttcacagcca tgcccctgtc aactcccttc tccctgaagc agacaactgc tacagccacc 
     2941 actacagcca catcagctcc tctctttact ggcctgggca ctgccacttc cacggtggcc 
     3001 tctggcaccg cagcttctgc ctcaaagcct gtctttggct ttggagtgac cactgcagcg 
     3061 agtactgcca gcagtaccat gacttccact tcccagtcag ttctgttcgg gggagccccc 
     3121 cctgttacta cttctagctc tgccccagct ctggcctcca tcttccagtt tggcaagcct 
     3181 cttgccccag cagcatctgc agcaggcacc tcctttagcc agccccttgc cagctctacc 
     3241 cagactgctg ccagcaatag tggcttcagt ggttttggca gcaccctcac cacctccacc 
     3301 tcggcccctg ccaccaccag tcagcccacg ctgaccttca gcaacactgt cacccccacc 
     3361 ttcaacattc cctttagctc cagtgccaag cctgcgctcc caacctaccc tggagccaac 
     3421 tcacagccca catttggagc caccgatggg gccaccaagc cagcacttgc cccaagtttt 
     3481 ggcagctcgt tcactttcgg caactctgtg gcctcagccc catcggcagc cccagcacca 
     3541 gccacctttg gcagtgctgc acagccagct ttcggagggt tgaaagccgc agcctccacc 
     3601 ttcggcgccc ctgccagcac tcagccagct tttggtagca ccacgtctgt gttctccttt 
     3661 gggtcagcta ccacatctgg ctttggtgct gctgccaccg ctgctaccac cacacagacc 
     3721 accaacagtg ggagcagcag ctctctgttt ggcagctctg ctccatcccc attcacattc 
     3781 ggtggctcag cagctcctgc tggcagtgga ggctttggcc ttagtgctac cccaggcacc 
     3841 agctccacct ctggaacttt cagctttgga tctggacaga gtgggacccc gggcaccacc 
     3901 acctcctttg gaagtctgag tcagaacacc ctgggcgcac ccagccaggg ctcacccttt 
     3961 gccttcagtg tgggcagtac acctgaaagc aagcctgtgt ttggaggcac atctacacct 
     4021 actttcgggc agagtgcccc tgcccccggc gtaggcacca cagggagcag cctctcattt 
     4081 ggggcctctt caacacctgc ccaaggcttt gttggagttg gaccttttgg atcagcagct 
     4141 ccttcgtttt ccattggtgc gggatccaag accccagggg ctcgacagcg acttcaggcc 
     4201 cgaaggcagc acacccgcaa gaagccaccg gtcgccacca tggtgagcaa gggcgaggca 
     4261 gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa cggccacgag 
     4321 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 
     4381 aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc tcagttcatg 
     4441 tacggctcca gggccttcac caagcacccc gccgacatcc ccgactacta taagcagtcc 
     4501 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgccgtgacc 
     4561 gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa gctccgcggc 
     4621 accaacttcc ctcctgacgg ccccgtaatg cagaagaaga caatgggctg ggaagcatcc 
     4681 accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat ggccctgcgc 
     4741 ctgaaggacg gcggccgcta cctggcggac ttcaagacca cctacaaggc caagaagccc 
     4801 gtgcagatgc ccggcgccta caacgtcgac cgcaagttgg acatcacctc ccacaacgag 
     4861 gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac cggcggcatg 
     4921 gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg 
     4981 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa 
     5041 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca 
     5101 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 
     5161 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc 
     5221 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc 
     5281 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag 
     5341 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc 
     5401 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa 
     5461 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg 
     5521 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt 
     5581 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc 
     5641 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 
     5701 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 
     5761 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt 
     5821 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 
     5881 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 
     5941 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 
     6001 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 
     6061 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 
     6121 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa 
     6181 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg 
     6241 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc 
     6301 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca 
     6361 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc 
     6421 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca 
     6481 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat 
     6541 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca 
     6601 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg 
     6661 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc 
     6721 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct 
     6781 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct 
     6841 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac 
     6901 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc 
     6961 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 
     7021 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 
     7081 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg 
     7141 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa 
     7201 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt 
     7261 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg 
     7321 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca 
     7381 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt 
     7441 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 
     7501 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 
     7561 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 
     7621 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 
     7681 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 
     7741 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 
     7801 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 
     7861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 
     7921 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 
     7981 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 
     8041 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 
     8101 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 
     8161 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 
     8221 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat 
//
