LOCUS       p-SUN1\mScarlet\        7396 bp    DNA     circular SYN  3-JUL-2019
DEFINITION  In vivo visualization of actin cytoskeleton (can be used for colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    pLifeAct_mScarlet_N1.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12883782|
COMMENT     VNTDBDATE|-12885392|
COMMENT     LSOWNER|
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COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                          "  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M," 
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COMMENT                          "  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular " 
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COMMENT                                           "Start: 3496  End: 3514 (Complementary)" 
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COMMENT                                           "Start: 3764  End: 3783" 1) 
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COMMENT                                           "Start: 4221  End: 4240 (Complementary)" 
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COMMENT                                     #160=(CLinePar 
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COMMENT                                           "SV40 polyA, reverse primer" 1) 
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COMMENT                                     #162=(CLinePar 
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COMMENT                                   "SV40pA-R") 
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COMMENT                                           "Start: 4275  End: 4294" 1) 
COMMENT                                     #165=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4449  End: 4470 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #170=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "F1 origin, forward primer" 1) 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #172=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                   "F1ori-F") 
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COMMENT                                     #174=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4661  End: 4680" 1) 
COMMENT                                     #175=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "F1 origin, reverse primer" 1) 
COMMENT                                     #176=(CLinePar 
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COMMENT                                     #177=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2009..2028" 1))) "F1ori-R") 
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COMMENT                                    (CObjectList 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4923  End: 4943 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #180=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "SV40 enhancer, reverse primer for pBABE vectors" 
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COMMENT                                     #181=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #182=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(2271..2291)" 1))) 
COMMENT                                   "pBABE 3'") 
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COMMENT                                    (CObjectList 
COMMENT                                     #184=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5170  End: 5189" 1) 
COMMENT                                     #185=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "SV40 promoter/origin, forward primer" 
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COMMENT                                    (CObjectList 
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COMMENT                                           "Start: 5346  End: 5365 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #190=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Neomycin resistance gene, reverse primer" 
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COMMENT                                     #191=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #192=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(2694..2713)" 1))) 
COMMENT                                   "Neo-R") 
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COMMENT                                    (CObjectList 
COMMENT                                     #194=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5956  End: 5975" 1) 
COMMENT                                     #195=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Neomycin resistance gene, forward primer" 
COMMENT                                           1) 
COMMENT                                     #196=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
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COMMENT                                           "   3304..3323" 1))) "Neo-F") 
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COMMENT                                    (CObjectList 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 6274  End: 6293 (Complementary)" 
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COMMENT                                     #200=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Thymidine kinase polyA, reverse primer" 
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COMMENT                                     #201=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #202=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(3622..3641)" 1))) 
COMMENT                                   "TK-pA-R") 
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COMMENT                                    (CObjectList 
COMMENT                                     #204=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7183  End: 7202" 1) 
COMMENT                                     #205=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "pBR322 origin, forward primer" 1) 
COMMENT                                     #206=(CLinePar 
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COMMENT                                     #207=(CLinePar 
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COMMENT                                           "   4531..4550" 1))) "pBR322ori-F"))) 
COMMENT                           "Primer Binding Site (13 total)") 
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COMMENT                                    (CObjectList 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 365   End: 568 " 1) 
COMMENT                                     #211=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "human cytomegalovirus (CMV) immediate early promoter" 
COMMENT                                           1) 
COMMENT                                     #212=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #213=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                    (CObjectList 
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COMMENT                                           "Start: 4794  End: 4898" 1) 
COMMENT                                     #216=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #217=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2142..2246" 1) 
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COMMENT                                     #219=(CLinePar 
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COMMENT                                           "   /gene=\"bla\"" 1))) 
COMMENT                                   "AmpR promoter") 
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COMMENT                                    (CObjectList 
COMMENT                                     #221=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4900  End: 5257" 1) 
COMMENT                                     #222=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "SV40 enhancer and early promoter" 1) 
COMMENT                                     #223=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #224=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   2248..2605" 1))) "SV40 promoter"))) 
COMMENT                           "Promoter Prokaryotic (3 total)") 
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COMMENT                            (CObjectList 
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COMMENT                                   (CGroupPar 
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COMMENT                                    (CObjectList 
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COMMENT                                           "Start: 4312  End: 4767 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #228=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" 
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COMMENT                                     #230=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(1660..2115)" 1) 
COMMENT                                     #231=(CLinePar 
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COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #232=(CLinePar 
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COMMENT                                           "   /direction=LEFT" 1))) "f1 ori") 
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COMMENT                                    (CObjectList 
COMMENT                                     #234=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5108  End: 5243" 1) 
COMMENT                                     #235=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "SV40 origin of replication" 1) 
COMMENT                                     #236=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #237=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                    (CObjectList 
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COMMENT                                           "Start: 6694  End: 7282" 1) 
COMMENT                                     #240=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #242=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4042..4630" 1) 
COMMENT                                     #243=(CLinePar 
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COMMENT                                     #244=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /direction=RIGHT" 1))) "ori"))) 
COMMENT                           "Replication Origin (3 total)"))) "Feature Map") 
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COMMENT                     (CObjectList 
COMMENT                      #246=(CRSFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 3311 " 1) 
COMMENT                                      #249=(CLinePar 
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COMMENT                                            " N2: 3351 " 1))))) "AgeI: 2 sites") 
COMMENT                            1 5 "ACCGGT" "TGGCCA") 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 3412 " 1) 
COMMENT                                      #253=(CLinePar 
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COMMENT                                            " N2: 7025 " 1))))) "ApaLI: 2 sites") 
COMMENT                            1 5 "GTGCAC" "CACGTG") 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 4235 " 1) 
COMMENT                                      #257=(CLinePar 
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COMMENT                                            " N2: 4339 " 1) 
COMMENT                                      #258=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 4350 " 1))))) "ApoI: 3 sites") 
COMMENT                            1 5 "RAATTY" "YTTAAR") 
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COMMENT                            (CFolderPar 
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COMMENT                            "CCGCGCGG") 
COMMENT                      #260=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 945 " 1) 
COMMENT                                      #263=(CLinePar 
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COMMENT                                            " N2: 1743 " 1) 
COMMENT                                      #264=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2013 " 1) 
COMMENT                                      #265=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 2578 " 1) 
COMMENT                                      #266=(CLinePar 
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COMMENT                                            " N5: 2954 " 1) 
COMMENT                                      #267=(CLinePar 
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COMMENT                                            " N6: 3628 " 1) 
COMMENT                                      #268=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 7) 1 2 2 0 191) 
COMMENT                                            " N7: 3820 " 1))))) "AvaI: 7 sites") 
COMMENT                            1 5 "CYCGRG" "GRGCYC") 
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COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 1044 " 1) 
COMMENT                                      #272=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #13# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1220 " 1) 
COMMENT                                      #273=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #13# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2925 " 1) 
COMMENT                                      #274=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #13# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 3270 " 1) 
COMMENT                                      #275=(CLinePar 
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COMMENT                                            " N5: 5937 " 1) 
COMMENT                                      #276=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #13# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 6382 " 1))))) "AvaII: 6 sites") 
COMMENT                            1 4 "GGWCC" "CCWGG") 
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COMMENT                            (CFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 3345 " 1))))) "BamHI: 1 site") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 91 " 1) 
COMMENT                                      #283=(CLinePar 
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COMMENT                                            " N2: 213 " 1) 
COMMENT                                      #284=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #14# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 284 " 1) 
COMMENT                                      #285=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #14# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 5196 " 1))))) "BglI: 4 sites") 
COMMENT                            7 4 "GCCNNNNNGGC" "CGGNNNNNCCG") 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 51499224 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
COMMENT                                      #288=(CLinePar 
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COMMENT                            1 5 "AGATCT" "TCTAGA") 
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FEATURES             Location/Qualifiers
     enhancer        61..364
                     /vntifkey="9"
                     /label=CMV\enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /vntifkey="30"
                     /label=CMV\promoter
                     /note="human cytomegalovirus (CMV) immediate early promoter"
     primer_bind     519..539
                     /vntifkey="28"
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3357..3366
                     /regulatory_class="other"
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="Unknown feature type:regulatory  vertebrate consensus sequence for strong initiation  of translation (Kozak, 1987)"
     CDS             3363..4058
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved
                     from a synthetic template (Bindels et al., 2016)"
                     /vntifkey="4"
                     /label=mScarlet
     primer_bind     complement(3496..3514)
                     /vntifkey="28"
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3764..3783
                     /vntifkey="28"
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4184..4305
                     /vntifkey="25"
                     /label=SV40\poly(A)\signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4221..4240)
                     /vntifkey="28"
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4275..4294
                     /vntifkey="28"
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4312..4767)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=f1\ori
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     primer_bind     complement(4449..4470)
                     /vntifkey="28"
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4661..4680
                     /vntifkey="28"
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4794..4898
                     /gene="bla"
                     /vntifkey="30"
                     /label=AmpR\promoter
     promoter        4900..5257
                     /vntifkey="30"
                     /label=SV40\promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(4923..4943)
                     /vntifkey="28"
                     /label=pBABE\3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5108..5243
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="SV40 origin of replication"
     primer_bind     5170..5189
                     /vntifkey="28"
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5292..6086
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /vntifkey="4"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))"
     primer_bind     complement(5346..5365)
                     /vntifkey="28"
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5956..5975
                     /vntifkey="28"
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6274..6293)
                     /vntifkey="28"
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6318..6365
                     /vntifkey="25"
                     /label=HSV\TK\poly(A)\signal
                     /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6694..7282
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     7183..7202
                     /vntifkey="28"
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             603..3341
                     /vntifkey="4"
                     /label=mSUN1\no\stop
     misc_feature    3342..3343
                     /vntifkey="21"
                     /label=+2bp
BASE COUNT     1783 a      1989 c      1986 g      1638 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca 
      601 ccatggactt ttctcggctg cacacgtaca ccccacccca gtgtgtgccg gagaacactg 
      661 gctacactta cgcactcagt tctagttact cgtcggatgc tctggatttt gaaactgagc 
      721 acaagttgga acctgtattt gactctccaa ggatgtcccg ccgcagcttg cgtctggtca 
      781 caacagcttc gtacagcagt ggggacagcc aggctattga ttcgcacatt agcaccagca 
      841 gggccacccc cgccaagggg agagaaacca ggacagtcaa acagagaaga agtgcaagca 
      901 agccagcttt tagtatcaac cacctgtcag ggaagggctt gtcctcgagc acaagccatg 
      961 acagctcttg cagcctgcgg agtgccacgg tgctgcggca ccctgtgcta gatgagtccc 
     1021 tgattcgtga gcagaccaaa gtggaccact tctggggtct cgatgatgat ggtgacctta 
     1081 aaggtggaaa taaagctgcc actcagggaa atggtgaact ggcagcagag gtggcgagca 
     1141 gcaatggata cacttgccgt gactgcagga tgctctcagc gcgcactgac gcactcacag 
     1201 cccactctgc catccacggg accacctcca gggtgtactc cagagacagg actctcaaac 
     1261 cacgcggagt gtccttttac ctggatagga ctctgtggct ggccaagtcc acctcctcat 
     1321 cctttgcatc atttatagtt caacttttcc aagtggtttt aatgaagctc aattttgaaa 
     1381 cttacaaatt gaaaggctat gaatccagag cttatgaatc acagagctat gagacaaaga 
     1441 gccatgagtc agaagcccat ctcggtcact gtgggaggat gactgccgga gaactttcca 
     1501 gagtggacgg ggagtccctg tgcgatgact gtaaggggaa gaagcacctt gagatacaca 
     1561 cagccaccca ctcgcaactg ccccagccac acagggtggc cggggccatg gggcgcctct 
     1621 gcatctatac aggtgacctc ttggttcaag cactgcgaag gactagagct gccgggtggt 
     1681 ctgtggccga ggccgtgtgg tcggtgctct ggctggctgt ctctgctcca gggaaggcag 
     1741 cctcgggaac cttctggtgg ctagggagcg gctggtacca atttgttact ttgatttctt 
     1801 ggctgaatgt ctttcttctt accaggtgcc ttcgaaatat ttgcaaggtt tttgtcttgc 
     1861 tcctcccact cctactttta ctaggtgctg gtgtctccct gtggggccag ggaaacttct 
     1921 tctcactcct accagtgctg aactggacgg ccatgcagcc aacacagagg gtggacgatt 
     1981 ccaagggcat gcatagacct ggccctcttc ccccgagccc acctccaaag gttgatcaca 
     2041 aggcttccca gtggcctcag gagagtgaca tggggcagaa ggtagcttct ttgagtgcgc 
     2101 agtgccacaa ccatgatgag agacttgcag agctgacagt cctgcttcag aaactacaga 
     2161 tacgggtaga ccaagtggat gacggcaggg aagggctgtc actgtgggtc aagaatgtgg 
     2221 ttggacagca cctgcaggag atgggcacca tagaaccacc tgatgctaag actgacttca 
     2281 tgactttcca ccatgaccat gaagtgcgtc tctccaactt ggaagatgtt cttagaaaac 
     2341 tgacagaaaa atctgaggct atccagaagg agctggaaga aaccaagctg aaagcaggca 
     2401 gcagggatga agagcagccc ctccttgacc gtgtgcagca cctagaactg gaactgaacc 
     2461 tgttgaagtc acagctgtca gactggcagc atctgaagac cagctgtgag caggctgggg 
     2521 cccgcatcca ggagactgtg cagctcatgt tctctgagga tcagcagggc ggttccctcg 
     2581 agtggctatt agagaagctt tcttctcggt tcgtgagcaa ggatgagctg caggtgctct 
     2641 tacatgacct tgagctgaaa ctgctgcaga atatcacaca ccacatcacc gtgacaggac 
     2701 aggccccgac atccgaggct attgtgtctg ccgtgaatca ggcagggatt tcaggaatca 
     2761 cagaagcgca agcacatatc attgtgaaca atgctctgaa gctgtactcc caagacaaga 
     2821 cggggatggt ggactttgct ctggagtctg gaggtggcag catcctaagc actcggtgct 
     2881 ctgagaccta tgagaccaag acggcactgc tgagcctgtt tggggtccca ctgtggtact 
     2941 tctcacagtc acctcgagtg gtgatccagc ccgacatcta cccagggaat tgctgggcgt 
     3001 tcaaaggttc ccaggggtac ctggtggtgc ggttgtccat gaagatctac ccaaccacat 
     3061 tcaccatgga acacattcca aagacactat cacccactgg taacatctcc agtgccccca 
     3121 aagactttgc agtctatgga ctggaaacgg agtatcaaga agaggggcag cctctgggac 
     3181 ggttcaccta tgaccaggaa ggagactcac tccagatgtt ccacacactg gaaagacctg 
     3241 accaagcctt ccagatagta gagctccggg tcctgtccaa ctggggccac cctgagtaca 
     3301 cttgcctcta ccggttccga gtccacggag agcccatcca gccggatcca ccggtcgcca 
     3361 ccatggtgag caagggcgag gcagtgatca aggagttcat gcggttcaag gtgcacatgg 
     3421 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg 
     3481 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttctcctggg 
     3541 acatcctgtc ccctcagttc atgtacggct ccagggcctt caccaagcac cccgccgaca 
     3601 tccccgacta ctataagcag tccttccccg agggcttcaa gtgggagcgc gtgatgaact 
     3661 tcgaggacgg cggcgccgtg accgtgaccc aggacacctc cctggaggac ggcaccctga 
     3721 tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta atgcagaaga 
     3781 agacaatggg ctgggaagca tccaccgagc ggttgtaccc cgaggacggc gtgctgaagg 
     3841 gcgacattaa gatggccctg cgcctgaagg acggcggccg ctacctggcg gacttcaaga 
     3901 ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc gaccgcaagt 
     3961 tggacatcac ctcccacaac gaggactaca ccgtggtgga acagtacgaa cgctccgagg 
     4021 gccgccactc caccggcggc atggacgagc tgtacaagta aagcggccgc gactctagat 
     4081 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct 
     4141 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc 
     4201 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 
     4261 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa 
     4321 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc 
     4381 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga 
     4441 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag 
     4501 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt 
     4561 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta 
     4621 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag 
     4681 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg 
     4741 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa 
     4801 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 
     4861 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct 
     4921 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 
     4981 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 
     5041 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 
     5101 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 
     5161 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 
     5221 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag 
     5281 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 
     5341 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 
     5401 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 
     5461 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 
     5521 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 
     5581 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 
     5641 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 
     5701 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 
     5761 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga 
     5821 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 
     5881 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 
     5941 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 
     6001 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 
     6061 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc 
     6121 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg 
     6181 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 
     6241 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga 
     6301 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt 
     6361 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc 
     6421 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg 
     6481 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta 
     6541 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 
     6601 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 
     6661 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 
     6721 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 
     6781 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 
     6841 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 
     6901 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 
     6961 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 
     7021 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 
     7081 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 
     7141 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 
     7201 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 
     7261 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 
     7321 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 
     7381 tattaccgcc atgcat 
//
