LOCUS       pLenti6.3-Lifeac        8500 bp    DNA     circular     28-MAR-2019
DEFINITION  attB1-attB2.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|609653909|
COMMENT     VNTDBDATE|-12745971|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLenti6.3-Lifeact-mApple|
COMMENT     VNTAUTHORNAME|Kathleen Clark|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pLenti6.3-Lifeact-mApple" 0 8500 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI" "AgeI" "ApoI" "AscI" "AvaII" "BglI" "BglII" 
COMMENT         "EcoRV" "KpnI" "NdeI" "NheI" "NotI" "PmeI" "PvuI" "PvuII" "SacI" 
COMMENT         "SacII" "SalI" "ScaI" "SpeI" "XbaI" "XhoI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (CMolPar 0 0 0 0 0 1 8500 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 4 342 0 3160 0 4406 0 7839 
COMMENT            0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 5 40 0 1058 0 6829 0 8055 0 
COMMENT            8247 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 7658 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 7053 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 7681 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 6 1781 0 5125 0 5437 0 
COMMENT            5993 0 6577 0 7058 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 7 541 0 944 0 1164 0 2158 0 
COMMENT            7373 0 7726 0 8212 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 7690 0 8134 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 1060 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 1058 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "AgeI" "accggt" 2 0 2 113 0 733 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "ApoI" "raatty" 2 0 10 152 0 2006 0 2872 0 
COMMENT             2883 0 5663 0 5750 0 7038 0 7045 0 7681 0 7752 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "AscI" "ggcgcgcc" 3 0 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 5 621 0 1325 0 3468 0 3690 
COMMENT             0 6836 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 6 2204 0 2447 0 3714 
COMMENT             0 7103 0 7225 0 7296 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "BglII" "agatct" 2 0 4 1616 0 1682 0 1723 0 
COMMENT             5379 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 19 0 7693 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 2 734 0 1584 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 7247 0 0 "") 
COMMENT        #19=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 2 34 0 6050 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 4 1401 0 2418 0 3463 0 
COMMENT             5251 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "PvuII" "cagctg" 4 0 5 1373 0 1611 0 2387 0 
COMMENT             4900 0 8357 0 0 "") 
COMMENT        #24=(CRSite (CStringList) "SacI" "gagctc" 6 0 5 1578 0 1741 0 5397 0 
COMMENT             5588 0 7581 0 0 "") 
COMMENT        #25=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 59 0 636 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 8376 0 0 "") 
COMMENT        #27=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 3351 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 7664 0 0 "") 
COMMENT        #29=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 46 0 7652 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 40 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 2 3232 0 5750 0 0 "")) 
COMMENT       (CObList 
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COMMENT             (CStringList) 1 1 1 1 "7698..7718") 
COMMENT        #33=(CFSignal (CObList) "attB2" 21 0 1 8493 13 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(8493..13)") 
COMMENT        #34=(CFSignal (CObList) "pUC origin" 33 0 0 4049 4722 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4049..4722") 
COMMENT        #35=(CFSignal (CObList) "Amp(R)" 4 0 0 3044 3904 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3044..3904") 
COMMENT        #36=(CFSignal (CObList) "bla promoter" 29 0 0 2945 3043 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2945..3043") 
COMMENT        #37=(CFSignal (CObList) "SV40 pA" 25 0 0 1955 2086 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1955..2086") 
COMMENT        #38=(CFSignal (CObList) "3'_LTR" 19 0 0 1703 1883 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1703..1883") 
COMMENT        #39=(CFSignal (CObList) "deltaU3" 19 0 0 1649 1883 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1649..1883") 
COMMENT        #40=(CFSignal (CObList) "Blasticidin resistance gene" 4 0 0 1165 1563 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1165..1563") 
COMMENT        #41=(CFSignal (CObList) "EM7 promoter" 30 0 0 1098 1164 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1098..1164") 
COMMENT        #42=(CFSignal (CObList) "SV40 promoter" 29 0 0 735 1043 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "735..1043") 
COMMENT        #43=(CFSignal (CObList) "WPRE" 21 0 0 127 724 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "127..724") 
COMMENT        #44=(CFSignal (CObList) "V5 epitope" 21 0 0 67 108 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "67..108") 
COMMENT        #45=(CFSignal (CObList) "CMV promoter" 29 0 0 7064 7648 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7064..7648") 
COMMENT        #46=(CFSignal (CObList) "cPPT" 21 0 0 6930 7052 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6930..7052") 
COMMENT        #47=(CFSignal (CObList) "3' splice acceptor" 38 0 0 6813 6813 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "6813..6813") 
COMMENT        #48=(CFSignal (CObList) "3' splice acceptor" 38 0 0 6785 6785 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "6785..6785") 
COMMENT        #49=(CFSignal (CObList) "HIV-1 Rev response element (RRE)" 21 0 0 6204 
COMMENT             6437 0 (CStringList) (CStringList) 1 1 1 1 "6204..6437") 
COMMENT        #50=(CFSignal (CObList) "HIC-1 psi packaging signal" 21 0 0 5650 5694 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5650..5694") 
COMMENT        #51=(CFSignal (CObList) "5' splice donor" 38 0 0 5649 5649 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5649..5649") 
COMMENT        #52=(CFSignal (CObList) "HIV-1 5' LTR" 19 0 0 5359 5539 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5359..5539") 
COMMENT        #53=(CFSignal (CObList) "RSV promoter" 29 0 0 5130 5358 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5130..5358") 
COMMENT        #54=(CFSignal (CObList) "mApple without stop" 4 0 0 7778 8485 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "7778..8485") 
COMMENT        #55=(CFSignal (CObList) "LifeAct" 4 0 0 7727 7777 0 (CStringList) 
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COMMENT                          "attB1-attB2." 1) 
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COMMENT                          "Foreign object. Author: Kathleen Clark. Original author: Kathleen Clark" 
COMMENT                          1) 
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COMMENT                          "Created: 12/16/11 04:18AM" 1) 
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COMMENT                          "Last Modified: 03/28/19 08:06AM" 1) 
COMMENT                     #64=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8500 bp" 1) 
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COMMENT                                           "   2945..3043" 1))) "bla promoter") 
COMMENT                             #150=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5130 (3 #53# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #151=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5130  End: 5358" 1) 
COMMENT                                     #152=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #153=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   5130..5358" 1))) "RSV promoter") 
COMMENT                             #154=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 7064 (3 #45# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #155=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7064  End: 7648" 1) 
COMMENT                                     #156=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #157=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   7064..7648" 1))) "CMV promoter"))) 
COMMENT                           "Promoter Eukaryotic (4 total)") 
COMMENT                     #158=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #159=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1098 (3 #41# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #160=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1098  End: 1164" 1) 
COMMENT                                     #161=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #162=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1098..1164" 1))) "EM7 promoter"))) 
COMMENT                           "Promoter Prokaryotic (1 total)") 
COMMENT                     #163=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #164=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4049 (3 #34# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #165=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4049  End: 4722" 1) 
COMMENT                                     #166=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #167=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4049..4722" 1))) "pUC origin"))) 
COMMENT                           "Replication Origin (1 total)") 
COMMENT                     #168=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 38 (7 38 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #169=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5649 (3 #51# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #170=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5649  End: 5649" 1) 
COMMENT                                     #171=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #172=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   5649..5649" 1))) 
COMMENT                                   "5' splice donor") 
COMMENT                             #173=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 6785 (3 #48# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #174=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 6785  End: 6785" 1) 
COMMENT                                     #175=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #176=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   6785..6785" 1))) 
COMMENT                                   "3' splice acceptor") 
COMMENT                             #177=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 6813 (3 #47# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #178=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 6813  End: 6813" 1) 
COMMENT                                     #179=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #180=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   6813..6813" 1))) 
COMMENT                                   "3' splice acceptor"))) 
COMMENT                           "Splicing Signal (3 total)"))) "Feature Map") 
COMMENT              #181=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #182=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 56025632 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #183=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #10# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #184=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #10# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 113 " 1) 
COMMENT                                      #185=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #10# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 733 " 1))))) "AgeI: 2 sites") 
COMMENT                            1 5 "ACCGGT" "TGGCCA") 
COMMENT                      #186=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 54393376 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #187=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #188=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 342 " 1) 
COMMENT                                      #189=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3160 " 1) 
COMMENT                                      #190=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 4406 " 1) 
COMMENT                                      #191=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 7839 " 1))))) "ApaLI: 4 sites") 
COMMENT                            1 5 "GTGCAC" "CACGTG") 
COMMENT                      #192=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 56025664 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #193=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #11# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #194=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 152 " 1) 
COMMENT                                      #195=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2006 " 1) 
COMMENT                                      #196=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2872 " 1) 
COMMENT                                      #197=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 2883 " 1) 
COMMENT                                      #198=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 5663 " 1) 
COMMENT                                      #199=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 5750 " 1) 
COMMENT                                      #200=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 7) 1 2 2 0 191) 
COMMENT                                            " N7: 7038 " 1) 
COMMENT                                      #201=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 8) 1 2 2 0 191) 
COMMENT                                            " N8: 7045 " 1) 
COMMENT                                      #202=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 9) 1 2 2 0 191) 
COMMENT                                            " N9: 7681 " 1) 
COMMENT                                      #203=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #11# 10) 1 2 2 0 
COMMENT                                             191) " N10: 7752 " 1))))) 
COMMENT                             "ApoI: 10 sites") 1 5 "RAATTY" "YTTAAR") 
COMMENT                      #204=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 56025696 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "AscI: 0 sites") 2 6 "GGCGCGCC" 
COMMENT                            "CCGCGCGG") 
COMMENT                      #205=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 54376128 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #206=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #207=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 40 " 1) 
COMMENT                                      #208=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1058 " 1) 
COMMENT                                      #209=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 6829 " 1) 
COMMENT                                      #210=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 8055 " 1) 
COMMENT                                      #211=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 8247 " 1))))) "AvaI: 5 sites") 
COMMENT                            1 5 "CYCGRG" "GRGCYC") 
COMMENT                      #212=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 56025728 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #213=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #13# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #214=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #13# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 621 " 1) 
COMMENT                                      #215=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #13# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1325 " 1) 
COMMENT                                      #216=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #13# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3468 " 1) 
COMMENT                                      #217=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #13# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 3690 " 1) 
COMMENT                                      #218=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #13# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 6836 " 1))))) "AvaII: 5 sites") 
COMMENT                            1 4 "GGWCC" "CCWGG") 
COMMENT                      #219=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 54381568 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #220=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #221=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7658 " 1))))) "BamHI: 1 site") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
COMMENT                      #222=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 56025760 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #223=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #14# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #224=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #14# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2204 " 1) 
COMMENT                                      #225=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #14# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2447 " 1) 
COMMENT                                      #226=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #14# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3714 " 1) 
COMMENT                                      #227=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #14# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 7103 " 1) 
COMMENT                                      #228=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #14# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 7225 " 1) 
COMMENT                                      #229=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #14# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 7296 " 1))))) "BglI: 6 sites") 
COMMENT                            7 4 "GCCNNNNNGGC" "CGGNNNNNCCG") 
COMMENT                      #230=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 56025792 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #231=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #15# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #232=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1616 " 1) 
COMMENT                                      #233=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1682 " 1) 
COMMENT                                      #234=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 1723 " 1) 
COMMENT                                      #235=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #15# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 5379 " 1))))) "BglII: 4 sites") 
COMMENT                            1 5 "AGATCT" "TCTAGA") 
COMMENT                      #236=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 54383776 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #237=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #238=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7053 " 1))))) "ClaI: 1 site") 
COMMENT                            2 4 "ATCGAT" "TAGCTA") 
COMMENT                      #239=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 54367328 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #240=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #241=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7681 " 1))))) "EcoRI: 1 site") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
COMMENT                      #242=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 56025824 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #243=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #16# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #244=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #16# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 19 " 1) 
COMMENT                                      #245=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #16# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 7693 " 1))))) "EcoRV: 2 sites") 
COMMENT                            3 3 "GATATC" "CTATAG") 
COMMENT                      #246=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 54384512 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #247=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #248=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1781 " 1) 
COMMENT                                      #249=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 5125 " 1) 
COMMENT                                      #250=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 5437 " 1) 
COMMENT                                      #251=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 5993 " 1) 
COMMENT                                      #252=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 6577 " 1) 
COMMENT                                      #253=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 7058 " 1))))) 
COMMENT                             "HindIII: 6 sites") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #254=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 56025856 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #255=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #17# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #256=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #17# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 734 " 1) 
COMMENT                                      #257=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #17# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1584 " 1))))) "KpnI: 2 sites") 
COMMENT                            5 1 "GGTACC" "CCATGG") 
COMMENT                      #258=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 54384928 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #259=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #260=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 541 " 1) 
COMMENT                                      #261=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 944 " 1) 
COMMENT                                      #262=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 1164 " 1) 
COMMENT                                      #263=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 2158 " 1) 
COMMENT                                      #264=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 7373 " 1) 
COMMENT                                      #265=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 7726 " 1) 
COMMENT                                      #266=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 7) 1 2 2 0 191) 
COMMENT                                            " N7: 8212 " 1))))) "NcoI: 7 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
COMMENT                      #267=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 56025888 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #268=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #18# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #269=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #18# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7247 " 1))))) "NdeI: 1 site") 
COMMENT                            2 4 "CATATG" "GTATAC") 
COMMENT                      #270=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 56025920 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" 
COMMENT                            "CGATCG") 
COMMENT                      #271=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 56025952 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #272=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #20# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #273=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #20# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 34 " 1) 
COMMENT                                      #274=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #20# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 6050 " 1))))) "NotI: 2 sites") 
COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
COMMENT                      #275=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 56025984 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "PmeI: 0 sites") 4 4 "GTTTAAAC" 
COMMENT                            "CAAATTTG") 
COMMENT                      #276=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 54371328 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #277=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #7# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #278=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7690 " 1) 
COMMENT                                      #279=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 8134 " 1))))) "PstI: 2 sites") 
COMMENT                            5 1 "CTGCAG" "GACGTC") 
COMMENT                      #280=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 56026016 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #281=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #22# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #282=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #22# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1401 " 1) 
COMMENT                                      #283=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #22# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2418 " 1) 
COMMENT                                      #284=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #22# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3463 " 1) 
COMMENT                                      #285=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #22# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 5251 " 1))))) "PvuI: 4 sites") 
COMMENT                            4 2 "CGATCG" "GCTAGC") 
COMMENT                      #286=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 56026048 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #287=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #23# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #288=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #23# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1373 " 1) 
COMMENT                                      #289=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #23# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1611 " 1) 
COMMENT                                      #290=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #23# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2387 " 1) 
COMMENT                                      #291=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #23# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 4900 " 1) 
COMMENT                                      #292=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #23# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 8357 " 1))))) "PvuII: 5 sites") 
COMMENT                            3 3 "CAGCTG" "GTCGAC") 
COMMENT                      #293=(CRSFolderPar 
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FEATURES             Location/Qualifiers
     misc_feature    7698..7718
                     /vntifkey="21"
                     /label=attB1
     misc_feature    complement(8493..13)
                     /vntifkey="21"
                     /label=attB2
     rep_origin      4049..4722
                     /vntifkey="33"
                     /label=pUC\origin
     CDS             3044..3904
                     /vntifkey="4"
                     /label=Amp(R)
     promoter        2945..3043
                     /vntifkey="29"
                     /label=bla\promoter
     polyA_signal    1955..2086
                     /vntifkey="25"
                     /label=SV40\pA
     LTR             1703..1883
                     /vntifkey="19"
                     /label=3'_LTR
     LTR             1649..1883
                     /vntifkey="19"
                     /label=deltaU3
     CDS             1165..1563
                     /vntifkey="4"
                     /label=Blasticidin\resistance\gene
     promoter        1098..1164
                     /vntifkey="30"
                     /label=EM7\promoter
     promoter        735..1043
                     /vntifkey="29"
                     /label=SV40\promoter
     misc_feature    127..724
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    67..108
                     /vntifkey="21"
                     /label=V5\epitope
     promoter        7064..7648
                     /vntifkey="29"
                     /label=CMV\promoter
     misc_feature    6930..7052
                     /vntifkey="21"
                     /label=cPPT
     splicing_signal 6813..6813
                     /vntifkey="38"
                     /label=3'\splice\acceptor
     splicing_signal 6785..6785
                     /vntifkey="38"
                     /label=3'\splice\acceptor
     misc_feature    6204..6437
                     /vntifkey="21"
                     /label=HIV-1\Rev\response\element\(RRE)
     misc_feature    5650..5694
                     /vntifkey="21"
                     /label=HIC-1\psi\packaging\signal
     splicing_signal 5649..5649
                     /vntifkey="38"
                     /label=5'\splice\donor
     LTR             5359..5539
                     /vntifkey="19"
                     /label=HIV-1\5'\LTR
     promoter        5130..5358
                     /vntifkey="29"
                     /label=RSV\promoter
     CDS             7778..8485
                     /vntifkey="4"
                     /label=mApple\without\stop
     CDS             7727..7777
                     /vntifkey="4"
                     /label=LifeAct
BASE COUNT     2186 a      2089 c      2165 g      2060 t 
ORIGIN
        1 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg 
       61 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggttag 
      121 taatgatcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 
      181 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 
      241 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 
      301 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 
      361 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 
      421 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 
      481 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc 
      541 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 
      601 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 
      661 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 
      721 ctggcgatgg taccggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 
      781 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 
      841 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 
      901 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 
      961 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg 
     1021 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt 
     1081 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa 
     1141 tacgacaagg tgaggaacta aaccatggcc aagcctttgt ctcaagaaga atccaccctc 
     1201 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc 
     1261 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact 
     1321 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac 
     1381 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg 
     1441 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat 
     1501 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc 
     1561 taagcacaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct 
     1621 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca 
     1681 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 
     1741 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 
     1801 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 
     1861 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 
     1921 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 
     1981 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 
     2041 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg 
     2101 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 
     2161 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 
     2221 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg 
     2281 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 
     2341 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 
     2401 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg 
     2461 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 
     2521 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 
     2581 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 
     2641 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 
     2701 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     2761 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 
     2821 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
     2881 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg 
     2941 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 
     3001 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 
     3061 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 
     3121 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 
     3181 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 
     3241 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 
     3301 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 
     3361 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 
     3421 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 
     3481 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 
     3541 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 
     3601 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 
     3661 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 
     3721 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 
     3781 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 
     3841 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 
     3901 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 
     3961 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 
     4021 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 
     4081 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 
     4141 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 
     4201 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 
     4261 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 
     4321 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 
     4381 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 
     4441 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 
     4501 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 
     4561 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 
     4621 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 
     4681 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 
     4741 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 
     4801 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 
     4861 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 
     4921 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc 
     4981 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca 
     5041 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta 
     5101 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct 
     5161 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca 
     5221 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt 
     5281 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc 
     5341 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 
     5401 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 
     5461 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 
     5521 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag 
     5581 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc 
     5641 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt 
     5701 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa 
     5761 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag 
     5821 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg 
     5881 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag 
     5941 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag 
     6001 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc 
     6061 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa 
     6121 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag 
     6181 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga 
     6241 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt 
     6301 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa 
     6361 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta 
     6421 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct 
     6481 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc 
     6541 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa 
     6601 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca 
     6661 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg 
     6721 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga 
     6781 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc 
     6841 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga 
     6901 ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg 
     6961 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta 
     7021 caaaaacaaa ttacaaaaat tcaaaatttt atcgataagc ttgggagttc cgcgttacat 
     7081 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa 
     7141 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg 
     7201 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc 
     7261 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct 
     7321 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga 
     7381 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa 
     7441 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc 
     7501 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg 
     7561 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac 
     7621 gctgttttga cctccataga agacaccgac tctagaggat ccactagtcc agtgtggtgg 
     7681 aattctgcag atatcaacaa gtttgtacaa aaaagcaggc ttaaccatgg gtgtcgcaga 
     7741 tttgatcaag aaattcgaaa gcatctcaaa ggaagaaatg gtgagcaagg gcgaggagaa 
     7801 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa 
     7861 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg cctttcagac 
     7921 cgctaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc 
     7981 tcagttcatg tacggctcca aggtctacat taagcaccca gccgacatcc ccgactactt 
     8041 caagctgtcc ttccccgagg gcttcaggtg ggagcgcgtg atgaacttcg aggacggcgg 
     8101 cattattcac gttaaccagg actcctccct gcaggacggc gtgttcatct acaaggtgaa 
     8161 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg 
     8221 ggaggcctcc gaggagcgga tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa 
     8281 gaggctgaag ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc 
     8341 caagaagccc gtgcagctgc ccggcgccta catcgtcgac atcaagttgg acatcgtgtc 
     8401 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac 
     8461 cggcggcatg gacgagctgt acaagtagta cccagctttc 
//
