LOCUS       pE(2F)-mApple\(L        3268 bp    DNA     circular     19-AUG-2009
DEFINITION  attL2-attL5.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|526983356|
COMMENT     VNTDBDATE|526983356|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pE(2F)-mApple (L5L2)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pE(2F)-mApple (L5L2)" 0 3268 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 55050361 0 "" 0 "" "" "" "" "" "" "pDONR221-P5P2" 
COMMENT             "" 0 0 1 2530 1 2530 2897 652 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 2 0 2897 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 652 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 1 1 0 80137288 0 "" 0 "tatacaaaagttgtg" "" "" "" "" "" "" 
COMMENT             "" 0 0 1 15 2531 2545 1 15 0 "tatacaaaagttgtg" "" "" "" "" "" 
COMMENT             (CObList) (CTerm 0 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 0 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 3 1 0 121 0 "" 0 "" "" "" "" "" "" "mApple" "" 0 0 1 708 
COMMENT             2546 3253 1 708 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 2 0 1 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 708 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #3=(CFragment 1 1 0 1073479801 0 "" 0 "tagtacccagctttc" "" "" "" "" "" 
COMMENT             "" "" 0 0 1 15 3254 3268 1 15 0 "tagtacccagctttc" "" "" "" "" "" 
COMMENT             (CObList) (CTerm 0 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 0 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 3268 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #4=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 2 1559 0 2607 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 2439 0 2823 0 3015 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 2980 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 103 0 2902 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "")) 
COMMENT       (CObList 
COMMENT        #14=(CFSignal (CObList) "attL2" 21 0 1 3261 88 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "attL5" 21 0 0 2448 2543 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "Km(R)" 4 0 0 272 1081 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1200000\"") 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "F1 origin" 33 0 0 1202 1875 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2500000\"") 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "M13 Reverse primer binding site" 28 0 1 143 159 
COMMENT             1 (CStringList) (CStringList "/invitrogen=\"2010000\"") 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "M13 Forward (-40) primer binding site" 31 0 0 
COMMENT             2395 2411 1 (CStringList) (CStringList "/invitrogen=\"2000000\"") 1 
COMMENT             1 1 1 "") 
COMMENT        #20=(CFSignal (CObList) "M13 Forward (-20) primer binding site" 28 0 0 
COMMENT             2415 2430 1 (CStringList) (CStringList "/invitrogen=\"1980000\"") 1 
COMMENT             1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "rrnB T2 transcription terminator" 43 0 1 2146 
COMMENT             2173 1 (CStringList) (CStringList "/invitrogen=\"2590000\"") 1 1 1 
COMMENT             1 "") 
COMMENT        #22=(CFSignal (CObList) "rrnB T1 transcription terminator" 43 0 1 2305 
COMMENT             2348 1 (CStringList) (CStringList "/invitrogen=\"2580000\"") 1 1 1 
COMMENT             1 "") 
COMMENT        #23=(CFSignal (CObList) "mApple" 4 0 0 2546 3253 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #24=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #25=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) 
COMMENT                    "pE(2F)-mApple (L5L2)" 2) 5 "" 0 4) 
COMMENT              #26=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pE(2F)-mApple (L5L2)'" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "attL2-attL5" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 08/19/09 08:15AM" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 08/19/09 08:15AM" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 3268 bp" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Constructed" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #35=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) 
COMMENT                   "Standard Fields") 
COMMENT              #36=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #37=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 11 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Generate PCR product using computed primers and a DNA polymerase (i.e. Platinum Pfx or Platinum Taq)." 
COMMENT                          1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Check quality and purify product by PEG/Mg2+ precipitation and agarose gels." 
COMMENT                          1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Mix purified PCR product and pDONR Vector pDONR221-P5P2 in BP buffer and TE buffers." 
COMMENT                          1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Thaw, vortex and add BP Clonase enzyme. Incubate >= 1 hr. at 25\260." 
COMMENT                          1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Add Proteinase K for 10 min at 37\260." 1) 
COMMENT                     #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "pDONR Vector and attB sequence carry identical resistance markers or pDONR vector is not adequately annotated." 
COMMENT                          1) 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Check annotation and if necessary use a different vector or construct the Entry Clone from a pDONR with different resistance." 
COMMENT                          1))) "Design Description") 
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COMMENT                    (CObjectList 
COMMENT                     #46=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 80878808 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #47=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 1559 " 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 2607 " 1))))) "ApaLI: 2 sites") 1 
COMMENT                          5 "GTGCAC" "CACGTG") 
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COMMENT                           (CGroupPar (CParagraph 80878104 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
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COMMENT                                  (CObjectList 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 2439 " 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 2823 " 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 3015 " 1))))) "AvaI: 3 sites") 1 5 
COMMENT                          "CYCGRG" "GRGCYC") 
COMMENT                     #55=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 80875992 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "BamHI: 0 sites") 1 5 "GGATCC" 
COMMENT                          "CCTAGG") 
COMMENT                     #56=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 80879224 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" 
COMMENT                          "TAGCTA") 
COMMENT                     #57=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 80885176 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "EcoRI: 0 sites") 1 5 "GAATTC" 
COMMENT                          "CTTAAG") 
COMMENT                     #58=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 80880440 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" 
COMMENT                          "TTCGAA") 
COMMENT                     #59=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 80874392 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #60=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #10# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #10# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 2980 " 1))))) "NcoI: 1 site") 1 5 
COMMENT                          "CCATGG" "GGTACC") 
COMMENT                     #62=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 80882008 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #63=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #11# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #11# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 103 " 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #11# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 2902 " 1))))) "PstI: 2 sites") 5 1 
COMMENT                          "CTGCAG" "GACGTC") 
COMMENT                     #66=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 80877592 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "SmaI: 0 sites") 3 3 "CCCGGG" 
COMMENT                          "GGGCCC") 
COMMENT                     #67=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 80873560 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList)) "XmaI: 0 sites") 1 5 "CCCGGG" 
COMMENT                          "GGGCCC"))) "Restriction/Methylation Map") 
COMMENT              #68=(CIvgnResourcePar 
COMMENT                   (CLinePar (CParagraph 1021 (0 0) 0 2 0 0 180) 
COMMENT                    "<a href=\"ivgnlink:/iq\">Invitrogen products related to pE(2F)-mApple (L5L2)</a>" 
COMMENT                    1) 2 "" 0 0) 
COMMENT              #69=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #70=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 10 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #71=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #0# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
COMMENT                            #72=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "parent position: from 2897 to 652" 1) 
COMMENT                            #73=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 2530" 1) 
COMMENT                            #74=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 1 to 2530" 1) 
COMMENT                            #75=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  Position 2897" 1))) "Left Terminus") 
COMMENT                            #77=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  Position 652" 1))) "Right Terminus"))) 
COMMENT                          "#1: FRAGMENT of <a href=\"vnti:dnarna/pDONR221-P5P2?sel=2897-652\">pDONR221-P5P2</a> ") 
COMMENT                     #79=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #1# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
COMMENT                            #80=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 2531 to 2545" 1))) 
COMMENT                          "#2: SEQUENCE TATACAAAAGTTGTG") 
COMMENT                     #81=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #2# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
COMMENT                            #82=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "parent position: from 1 to 708" 1) 
COMMENT                            #83=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 708" 1) 
COMMENT                            #84=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 2546 to 3253" 1) 
COMMENT                            #85=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  Position 1" 1))) "Left Terminus") 
COMMENT                            #87=(CFolderPar 
COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  Position 708" 1))) "Right Terminus"))) 
COMMENT                          "#3: FRAGMENT of <a href=\"vnti:dnarna/mApple?sel=1-708\">mApple</a> ") 
COMMENT                     #89=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (5 #3# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
COMMENT                            #90=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 3254 to 3268" 1))) 
COMMENT                          "#4: SEQUENCE TAGTACCCAGCTTTC"))) 
COMMENT                   "Component Fragments") 
COMMENT              #91=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #92=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #93=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 272 (3 #16# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 272   End: 1081" 1))) "Km(R)") 
COMMENT                            #95=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2546  End: 3253" 1))) "mApple"))) 
COMMENT                          "CDS (2 total)") 
COMMENT                     #97=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                  (CObjectList 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2448  End: 2543" 1))) "attL5") 
COMMENT                            #100=(CFolderPar 
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COMMENT                                   (CParagraph 3261 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #101=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 3261  End: 88   (Complementary)" 
COMMENT                                          1))) "attL2"))) 
COMMENT                          "Misc. Feature (2 total)") 
COMMENT                     #102=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 28 (7 28 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #103=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 143 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #104=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 143   End: 159  (Complementary)" 
COMMENT                                           1))) 
COMMENT                                   "M13 Reverse primer binding site") 
COMMENT                             #105=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2415 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #106=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2415  End: 2430" 1))) 
COMMENT                                   "M13 Forward (-20) primer binding site"))) 
COMMENT                           "Primer Binding Site (2 total)") 
COMMENT                     #107=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 31 (7 31 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #108=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2395 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2395  End: 2411" 1))) 
COMMENT                                   "M13 Forward (-40) primer binding site"))) 
COMMENT                           "Protein Binding Site (1 total)") 
COMMENT                     #110=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #111=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1202 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1202  End: 1875" 1))) 
COMMENT                                   "F1 origin"))) "Replication Origin (1 total)") 
COMMENT                     #113=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #114=(CFolderPar 
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COMMENT                                    (CObjectList 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2146  End: 2173 (Complementary)" 
COMMENT                                           1))) 
COMMENT                                   "rrnB T2 transcription terminator") 
COMMENT                             #116=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2305 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2305  End: 2348 (Complementary)" 
COMMENT                                           1))) 
COMMENT                                   "rrnB T1 transcription terminator"))) 
COMMENT                           "Terminator (2 total)"))) "Feature Map")))) 
COMMENT       (CGraphView 
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COMMENT          #119=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #120=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #121=(CWidgetStyle "Molecule Label 1" 0 0 1 
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COMMENT                "@N" 0) 
COMMENT          #122=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #123=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
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COMMENT          #124=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
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COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
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FEATURES             Location/Qualifiers
     misc_feature    complement(3261..88)
                     /vntifkey="21"
                     /label=attL2
     misc_feature    2448..2543
                     /vntifkey="21"
                     /label=attL5
     CDS             272..1081
                     /invitrogen="1200000"
                     /vntifkey="4"
                     /label=Km(R)
     rep_origin      1202..1875
                     /invitrogen="2500000"
                     /vntifkey="33"
                     /label=F1\origin
     primer_bind     complement(143..159)
                     /invitrogen="2010000"
                     /vntifkey="28"
                     /label=M13\Reverse\primer\binding\site
     protein_bind    2395..2411
                     /invitrogen="2000000"
                     /vntifkey="31"
                     /label=M13\Forward\(-40)\primer\binding\site
     primer_bind     2415..2430
                     /invitrogen="1980000"
                     /vntifkey="28"
                     /label=M13\Forward\(-20)\primer\binding\site
     terminator      complement(2146..2173)
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     terminator      complement(2305..2348)
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     CDS             2546..3253
                     /vntifkey="4"
                     /label=mApple
BASE COUNT      809 a       825 c       845 g       789 t 
ORIGIN
        1 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc 
       61 actatcagtc aaaataaaat cattatttgc catccagctg cagggcggcc gcgatatccc 
      121 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca 
      181 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc 
      241 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag 
      301 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa 
      361 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt 
      421 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact 
      481 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga 
      541 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga 
      601 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca 
      661 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc 
      721 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg 
      781 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc 
      841 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat 
      901 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct 
      961 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg 
     1021 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta 
     1081 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg 
     1141 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga 
     1201 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     1261 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     1321 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 
     1381 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     1441 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     1501 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     1561 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     1621 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     1681 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     1741 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     1801 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     1861 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 
     1921 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 
     1981 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 
     2041 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 
     2101 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc 
     2161 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac 
     2221 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg 
     2281 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt 
     2341 cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc 
     2401 ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa taatgatttt 
     2461 attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat 
     2521 gccaactttg tatacaaaag ttgtgatggt gagcaagggc gaggagaata acatggccat 
     2581 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt 
     2641 cgagatcgag ggcgagggcg agggccgccc ctacgaggcc tttcagaccg ctaagctgaa 
     2701 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta 
     2761 cggctccaag gtctacatta agcacccagc cgacatcccc gactacttca agctgtcctt 
     2821 ccccgagggc ttcaggtggg agcgcgtgat gaacttcgag gacggcggca ttattcacgt 
     2881 taaccaggac tcctccctgc aggacggcgt gttcatctac aaggtgaagc tgcgcggcac 
     2941 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccga 
     3001 ggagcggatg taccccgagg acggcgccct gaagagcgag atcaagaaga ggctgaagct 
     3061 gaaggacggc ggccactacg ccgccgaggt caagaccacc tacaaggcca agaagcccgt 
     3121 gcagctgccc ggcgcctaca tcgtcgacat caagttggac atcgtgtccc acaacgagga 
     3181 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga 
     3241 cgagctgtac aagtagtacc cagctttc 
//
