LOCUS       Exported                6748 bp DNA     circular SYN 26-JUN-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   gene
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      26..73
                     /label=T7 terminator
                     /note="color: #ffffff"
     CDS             complement(140..157)
                     /codon_start=1
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     misc_feature    158..166
                     /label=MCS
                     /note="color: #99ccff"
     CDS             complement(1628..1651)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /note="color: #cc99b2"
                     /translation="LEVLFQGP"
     CDS             complement(1655..1672)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             complement(1676..1678)
                     /codon_start=1
                     /label=ATG
                     /note="color: #993366"
                     /translation="M"
     RBS             1686..1691
                     /note="color: #a6acb3"
     protein_bind    1722..1746
                     /label=lac operator
                     /note="color: #31849b"
     promoter        complement(1747..1765)
                     /label=T7 promoter
                     /note="color: #ffffff; direction: LEFT"
     promoter        2074..2151
                     /label=lacI promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2152..3234
                     /codon_start=1
                     /label=lacI
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             4010..4234
                     /codon_start=1
                     /label=rop
                     /note="color: #993366"
                     /translation="MNRNPPYTEASVTKQEKTALNMARFIRSQTLTLLEKLNELDADEQ
                     ADICESLHDHADELYRSCLARFGDDGENL"
     rep_origin      complement(4664..5252)
                     /direction=LEFT
                     /label=ori
                     /note="color: #ffff00"
     CDS             5374..6189
                     /codon_start=1
                     /label=KanR
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(6282..6737)
                     /direction=LEFT
                     /label=f1 ori
                     /note="color: #ffff00"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcttaatcca
      181 ccccttcaaa tccttgagca ttttccacgg ccatgagaag tttctctcgt aaatcttcaa
      241 aggtttcata tggaggtaag tcaaggcgat taaagcatgt gtgagctctg ggcagtttct
      301 caggactgcc ccattgctct attgtaaaca gctgaggacc attggaacca taaagttcgg
      361 caaatccatt cataggtact cgcgatgtcc ctgtgacaaa ctgcagtaac cggatacgct
      421 tttcggcgtc catgagtagc acagccttcc agaaccactg aatgacgggg tggtttgggc
      481 agtagccgtt cttgtaaata gaatgctgtc tccagtcatt cacatccaca tcaccgaggc
      541 cgcacatgag caactccagc tcattttcgt caaaaatttt aatcaaatca ataggaagta
      601 gttctgtgaa tccctccaag aaggcgttca tctgcttctg gaccctgttc acaaatctcc
      661 actggatgac taagtcgata tattcccttt tgttttcatt tgtgaccatt atttctgacc
      721 cattgggctt caaatccact tgatatgtct gtccaaagtt ttcttcgtct atgcagaaca
      781 tgaggtccag ctcagtaggg tcattctcca ggatccattt caaagagttg taatattcac
      841 tatccacaga ttccatgtca ttcagggtta tctgctttcc caacatcatc ttgtaaaatg
      901 gtctaatgaa gaaaccatct aagagcttcc catgaaatac ggccagacca gcaactcttc
      961 caataaaagt gaagtaggac aaatgatcct cattacagag gcctgaatta gggttgatct
     1021 gaagggtgta gttgtccgtg gcagagtact caaagaggcc gtagtagggg ttgaacatct
     1081 ctttggacag taagaagaac cattctctgg ccacaccccc atagtcaaga cctttctctg
     1141 attcaaactc aatccacagt ctagctttta ggacatctgg tcttttcacg gacataattc
     1201 tccgatagga ctcttcaaat atgttatttc tgtgaagttt catttcaaac ctattgggga
     1261 tatcagcagg tttctttaat ttcttcctga agtagtcata tttctgctta aattctctgg
     1321 agtaagggac agccggacca gtaatagctg ggttctgcag tcttgggtct tcccactgag
     1381 taattttgct attatgatca atataaaacg ttcggccatc caagtgaatt ctttcttccc
     1441 agccaggagg aagggggcca aggtcattgg ggtttaaaga tgtctttgac cgcatatgta
     1501 ctggaaattt caaacgtgga tcttcccagg ttgtagtctt tgtgttatga tcaatgaaga
     1561 agggccggcc gtttggcgct atcctcattt cccagccggg tggcaagaag ctctgtgtga
     1621 ctttgtgtgg gccctggaac agcacctcca ggccgtggtg gtggtggtgg tgccccatgg
     1681 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
     1741 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg
     1801 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     1861 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
     1921 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
     1981 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
     2041 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt
     2101 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca
     2161 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
     2221 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
     2281 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg
     2341 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
     2401 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
     2461 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
     2521 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
     2581 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
     2641 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
     2701 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
     2761 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
     2821 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
     2881 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
     2941 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
     3001 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     3061 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
     3121 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     3181 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     3241 cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag
     3301 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     3361 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
     3421 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
     3481 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg
     3541 cgagaagcag gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct
     3601 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
     3661 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
     3721 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg
     3781 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac
     3841 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat
     3901 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt
     3961 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa
     4021 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
     4081 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga
     4141 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct
     4201 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     4261 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     4321 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     4381 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa
     4441 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     4501 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4561 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4621 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4681 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4741 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4801 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4861 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4921 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4981 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5041 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5101 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5161 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5221 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5281 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaac aataaaactg
     5341 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct
     5401 tgctctaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     5461 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     5521 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     5581 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     5641 actcctgatg atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta
     5701 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     5761 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     5821 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     5881 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     5941 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     6001 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     6061 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     6121 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     6181 tttttctaag aattaattca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6241 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaaattgtaa acgttaatat
     6301 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     6361 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     6421 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     6481 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     6541 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     6601 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     6661 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     6721 gccgctacag ggcgcgtccc attcgcca
//
