LOCUS       Exported                7901 bp DNA     circular SYN 29-JAN-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   gene
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
                     /note="This forward directional feature has 3 segments:
                        1: 183 .. 188 / #ffffff / -35
                        2: 189 .. 204 / #ffffff
                        3: 205 .. 211 / #ffffff / -10"
     protein_bind    219..235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     CDS             258..911
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /note="color: #cc99b2"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     869..891
                     /label=pGEX 5' Sequencing Primer
                     /note="color: black; sequence: GGGCTGGCAAGCCACGTTTGGTG"
     CDS             918..939
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=PreScission site
                     /note="color: #cc99b2
                     Cleavage site after base 935"
                     /translation="LEVLFQG"
     misc_feature    3903..3913
                     /label=stop codons
                     /note="stop codons in all three reading frames"
                     /note="color: #ff0000"
     primer_bind     complement(3951..3973)
                     /label=pGEX 3' Sequencing Primer
                     /note="color: black; sequence: CCGGGAGCTGCATGTGTCAGAGG"
     promoter        4204..4308
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4309..5169
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 4309 .. 4377 / #ccffcc / signal sequence
                        2: 4378 .. 5169 / #ccffcc
                     Cleavage site after base 4377"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5340..5928
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        6172..6249
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6250..7332
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggagta cgcgccggct
      961 ccatggcgac cgggctcggg gagccggtct atggactttc cgaagacgag ggagagtccc
     1021 gtattctcag agtaaaagtt gtttctggaa ttgatctcgc caaaaaggac atctttggag
     1081 ccagtgatcc gtatgtgaaa ctttcattgt acgtagcgga tgagaataga gaacttgctt
     1141 tggtccagac aaaaacaatt aaaaagacac tgaacccaaa atggaatgaa gaattttatt
     1201 tcagggtaaa cccatctaat cacagactcc tatttgaagt atttgacgaa aatagactga
     1261 cacgagacga cttcctgggc caggtggacg tgccccttag tcaccttccg acagaagatc
     1321 caaccatgga gcgaccctat acatttaagg actttctcct cagaccaaga agtcataagt
     1381 ctcgagttaa gggatttttg cgattgaaaa tggcctatat gccaaaaaat ggaggtcaag
     1441 atgaagaaaa cagtgaccag agggatgaca tggagcatgg atgggaagtt gttgactcaa
     1501 atgactcggc ttctcagcac caagaggaac ttcctcctcc tcctctgcct cccgggtggg
     1561 aagaaaaagt ggacaattta ggccgaactt actatgtcaa ccacaacaac cggaccactc
     1621 agtggcacag accaagcctg atggacgtgt cctcggagtc ggacaataac atcagacaga
     1681 tcaaccagga ggcagcacac cggcgcttcc gctcccgcag gcacatcagc gaagacttgg
     1741 agcccgagcc ctcggagggc ggggatgtcc ccgagccttg ggagaccatt tcagaggaag
     1801 tgaatatcgc tggagactct ctcggtctgg ctctgccccc accaccggcc tccccaggat
     1861 ctcggaccag ccctcaggag ctgtcagagg aactaagcag aaggcttcag atcactccag
     1921 actccaatgg ggaacagttc agctctttga ttcaaagaga accctcctca aggttgaggt
     1981 catgcagtgt caccgacgca gttgcagaac agggccatct accaccgccc agtgccccag
     2041 ctgggagagc gcgttcatca actgtcacgg gtggtgagga accaacgcca tcagtggcct
     2101 atgtacatac tacgccgggt ctgccttcag gctgggaaga aagaaaagat gctaaggggc
     2161 gcacatacta tgtcaatcat aacaatcgaa ccacaacttg gactcgacct atcatgcagc
     2221 ttgcagaaga tggtgcgtcc ggatcagcca caaacagtaa caaccatcta atcgagcctc
     2281 agatccgccg gcctcgtagc ctcagctcgc caacagtaac tttatctgcc ccgctggagg
     2341 gtgccaagga ctcacccgta cgtcgggctg tgaaagacac cctttccaac ccacagtccc
     2401 cacagccatc accttacaac tcccccaaac cacaacacaa agtcacacag agcttcttgc
     2461 cacccggctg ggaaatgagg atagcgccaa acggccggcc cttcttcatt gatcataaca
     2521 caaagactac aacctgggaa gatccacgtt tgaaatttcc agtacatatg cggtcaaaga
     2581 catctttaaa ccccaatgac cttggccccc ttcctcctgg ctgggaagaa agaattcact
     2641 tggatggccg aacgttttat attgatcata atagcaaaat tactcagtgg gaagacccaa
     2701 gactgcagaa cccagctatt actggtccgg ctgtccctta ctccagagaa tttaagcaga
     2761 aatatgacta cttcaggaag aaattaaaga aacctgctga tatccccaat aggtttgaaa
     2821 tgaaacttca cagaaataac atatttgaag agtcctatcg gagaattatg tccgtgaaaa
     2881 gaccagatgt cctaaaagct agactgtgga ttgagtttga atcagagaaa ggtcttgact
     2941 atgggggtgt ggccagagaa tggttcttct tactgtccaa agagatgttc aacccctact
     3001 acggcctctt tgagtactct gccacggaca actacaccct tcagatcaac cctaattcag
     3061 gcctctgtaa tgaggatcat ttgtcctact tcacttttat tggaagagtt gctggtctgg
     3121 ccgtatttca tgggaagctc ttagatggtt tcttcattag accattttac aagatgatgt
     3181 tgggaaagca gataaccctg aatgacatgg aatctgtgga tagtgaatat tacaactctt
     3241 tgaaatggat cctggagaat gaccctactg agctggacct catgttctgc atagacgaag
     3301 aaaactttgg acagacatat caagtggatt tgaagcccaa tgggtcagaa ataatggtca
     3361 caaatgaaaa caaaagggaa tatatcgact tagtcatcca gtggagattt gtgaacaggg
     3421 tccagaagca gatgaacgcc ttcttggagg gattcacaga actacttcct attgatttga
     3481 ttaaaatttt tgatgaaaat gagctggagt tgctcatgtg cggcctcggt gatgtggatg
     3541 tgaatgactg gagacagcat tctatttaca agaacggcta ctgcccaaac caccccgtca
     3601 ttcagtggtt ctggaaggct gtgctactca tggacgccga aaagcgtatc cggttactgc
     3661 agtttgtcac agggacatcg cgagtaccta tgaatggatt tgccgaactt tatggttcca
     3721 atggtcctca gctatttaca atagagcaat ggggcagtcc tgagaaactg cccagagctc
     3781 acacaagttt taatcgcctt gacttacctc catatgaaac ctttgaagat ttacgagaga
     3841 aacttctcat ggccgtggaa aatgctcaag gatttgaagg ggtggattaa gcggccgcat
     3901 cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     3961 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     4021 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     4081 agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     4141 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4201 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4261 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4321 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4381 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4441 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4501 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     4561 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4621 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4681 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4741 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4801 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     4861 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4921 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4981 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5041 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5101 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5161 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5221 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5281 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5341 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5401 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5461 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     5521 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5581 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5641 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5701 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5761 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5821 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5881 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5941 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6001 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6061 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     6121 cggtattttc tccttacgca tctgtgcggt atttcacacc gcataaattc cgacaccatc
     6181 gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
     6241 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
     6301 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
     6361 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
     6421 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
     6481 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
     6541 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
     6601 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
     6661 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
     6721 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
     6781 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
     6841 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
     6901 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
     6961 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
     7021 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
     7081 gacagctcat gttatatccc gccgtcaacc accatcaaac aggattttcg cctgctgggg
     7141 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     7201 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     7261 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7321 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7381 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     7441 cacaggaaac agctatgacc atgattacgg attcactggc cgtcgtttta caacgtcgtg
     7501 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     7561 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     7621 atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg
     7681 agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt
     7741 acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg ccgtttgttc
     7801 ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac
     7861 aggaaggcca gacgcgaatt atttttgatg gcgttggaat t
//
