LOCUS       Exported                7248 bp DNA     circular SYN 18-DEC-2019
DEFINITION  Vector for expressing 6xHis-TEV-tagged proteins in insect cells 
            using the Bac-to-Bac(R) baculovirus system. For other reading 
            frames, use pFastBac(TM)HT B or pFastBac(TM)HT C.
ACCESSION   .
VERSION     .
KEYWORDS    His-TEV-NEDD4L_pfastb
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
COMMENT     Transform into DH10Bac(TM) E. coli cells.
FEATURES             Location/Qualifiers
     source          1..7248
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; 
                     arrowindicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        484..588
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
                     /note="color: #ffffff"
     CDS             589..1449
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7
                     /note="Tn7R"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
                     /note="color: #a6acb3"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=aacC1
                     /note="GmR"
                     /note="confers resistance to gentamycin"
                     /note="color: #993366"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3524..3552)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=intI1 (promoter lies within the coding sequence
                     /note="Pc promoter"
                     /note="class 1 integron promoter"
                     /note="color: #ffffff; direction: LEFT"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /label=polh from Autographa californica promoter
                     /note="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
                     /note="color: #ffffff"
     CDS             4050..6560
                     /codon_start=1
                     /label=1- His-TEV-NEDD4L_C2_W1_L_w2Hect
                     /note="1- His-TEV-NEDD4L_C2_W1_L_w2Hect"
                     /note="color: #993366"
                     /translation="MSYYHHHHHHDYDIPTTENLYFQGMATGLGEPVYGLSEDEGESRI
                     LRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYF
                     RVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHK
                     SRVKGFLRLKMAYMPKNGGQDEENSDQRDDMEHGWEVVDSNDSASQHQEELPPPPLPPG
                     WEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISE
                     DLEPEPSEGGDVPEPWETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELSRRLQ
                     ITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPPSAPAGRARSSTVTGGEEPT
                     PSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQNPAITGPAVPYSREF
                     KQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEK
                     GLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGR
                     VAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLM
                     FCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTE
                     LLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDA
                     EKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPY
                     ETFEDLREKLLMAVENAQGFEGVD"
     polyA_signal    6738..6872
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     mobile_element  6901..7066
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7
                     /note="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
                     /note="color: #a6acb3"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg
     4081 attacgatat cccaacgacc gaaaacctgt attttcaggg catggcgacc gggctcgggg
     4141 agccggtcta tggactttcc gaagacgagg gagagtcccg tattctcaga gtaaaagttg
     4201 tttctggaat tgatctcgcc aaaaaggaca tctttggagc cagtgatccg tatgtgaaac
     4261 tttcattgta cgtagcggat gagaatagag aacttgcttt ggtccagaca aaaacaatta
     4321 aaaagacact gaacccaaaa tggaatgaag aattttattt cagggtaaac ccatctaatc
     4381 acagactcct atttgaagta tttgacgaaa atagactgac acgagacgac ttcctgggcc
     4441 aggtggacgt gccccttagt caccttccga cagaagatcc aaccatggag cgaccctata
     4501 catttaagga ctttctcctc agaccaagaa gtcataagtc tcgagttaag ggatttttgc
     4561 gattgaaaat ggcctatatg ccaaaaaatg gaggtcaaga tgaagaaaac agtgaccaga
     4621 gggatgacat ggagcatgga tgggaagttg ttgactcaaa tgactcggct tctcagcacc
     4681 aagaggaact tcctcctcct cctctgcctc ccgggtggga agaaaaagtg gacaatttag
     4741 gccgaactta ctatgtcaac cacaacaacc ggaccactca gtggcacaga ccaagcctga
     4801 tggacgtgtc ctcggagtcg gacaataaca tcagacagat caaccaggag gcagcacacc
     4861 ggcgcttccg ctcccgcagg cacatcagcg aagacttgga gcccgagccc tcggagggcg
     4921 gggatgtccc cgagccttgg gagaccattt cagaggaagt gaatatcgct ggagactctc
     4981 tcggtctggc tctgccccca ccaccggcct ccccaggatc tcggaccagc cctcaggagc
     5041 tgtcagagga actaagcaga aggcttcaga tcactccaga ctccaatggg gaacagttca
     5101 gctctttgat tcaaagagaa ccctcctcaa ggttgaggtc atgcagtgtc accgacgcag
     5161 ttgcagaaca gggccatcta ccaccgccca gtgccccagc tgggagagcg cgttcatcaa
     5221 ctgtcacggg tggtgaggaa ccaacgccat cagtggccta tgtacatact acgccgggtc
     5281 tgccttcagg ctgggaagaa agaaaagatg ctaaggggcg cacatactat gtcaatcata
     5341 acaatcgaac cacaacttgg actcgaccta tcatgcagaa cccagctatt actggtccgg
     5401 ctgtccctta ctccagagaa tttaagcaga aatatgacta cttcaggaag aaattaaaga
     5461 aacctgctga tatccccaat aggtttgaaa tgaaacttca cagaaataac atatttgaag
     5521 agtcctatcg gagaattatg tccgtgaaaa gaccagatgt cctaaaagct agactgtgga
     5581 ttgagtttga atcagagaaa ggtcttgact atgggggtgt ggccagagaa tggttcttct
     5641 tactgtccaa agagatgttc aacccctact acggcctctt tgagtactct gccacggaca
     5701 actacaccct tcagatcaac cctaattcag gcctctgtaa tgaggatcat ttgtcctact
     5761 tcacttttat tggaagagtt gctggtctgg ccgtatttca tgggaagctc ttagatggtt
     5821 tcttcattag accattttac aagatgatgt tgggaaagca gataaccctg aatgacatgg
     5881 aatctgtgga tagtgaatat tacaactctt tgaaatggat cctggagaat gaccctactg
     5941 agctggacct catgttctgc atagacgaag aaaactttgg acagacatat caagtggatt
     6001 tgaagcccaa tgggtcagaa ataatggtca caaatgaaaa caaaagggaa tatatcgact
     6061 tagtcatcca gtggagattt gtgaacaggg tccagaagca gatgaacgcc ttcttggagg
     6121 gattcacaga actacttcct attgatttga ttaaaatttt tgatgaaaat gagctggagt
     6181 tgctcatgtg cggcctcggt gatgtggatg tgaatgactg gagacagcat tctatttaca
     6241 agaacggcta ctgcccaaac caccccgtca ttcagtggtt ctggaaggct gtgctactca
     6301 tggacgccga aaagcgtatc cggttactgc agtttgtcac agggacatcg cgagtaccta
     6361 tgaatggatt tgccgaactt tatggttcca atggtcctca gctatttaca atagagcaat
     6421 ggggcagtcc tgagaaactg cccagagctc acacatgctt taatcgcctt gacttacctc
     6481 catatgaaac ctttgaagat ttacgagaga aacttctcat ggccgtggaa aatgctcaag
     6541 gatttgaagg ggtggattaa gcggccgctt tcgaatctag agcctgcagt ctcgaggcat
     6601 gcggtaccaa gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag
     6661 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     6721 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6781 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6841 aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct aggagatccg
     6901 aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct
     6961 tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa
     7021 ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg ggcatttttc
     7081 ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc gtcatcttcg
     7141 gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt attaatgttt
     7201 gtaattgact gaatatcaac gcttatttgc agcctgaatg gcgaatgg
//
