LOCUS       Exported                1149 bp ds-DNA     linear   UNA 13-MAY-2021
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 25, 2021 from SnapGene 5.1.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     regulatory      1..6
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7..444
                     /codon_start=1
                     /label=TVA
                     /translation="MARLLPALLLLLLPGNVTGNGSGNGSLSRCPPGQFRCSEPPGAHG
                     ECYPQDWLCDGHPDCDDGRDEWGCGTSAIPAVPTDNGTEAPTAPAPGRALPARNHGRMW
                     MLITAVLLCCLVAVGGIAAWGKSKAKSRSDIFSLESASKGGG"
     CDS             445..1149
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(587..605)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     855..874
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gccaccatgg cgcggctgct gcccgcgctg ctgctgctgc tgctgcccgg taacgtgacc
       61 ggtaacgggt ccggtaacgg ttctttgtcc cgttgccccc ccggtcagtt ccgctgctcg
      121 gagccgcccg gtgcccacgg ggagtgttac ccgcaggact ggctgtgcga cggacacccc
      181 gactgcgacg acgggcggga cgagtggggc tgcgggacca gcgcgatccc cgcggtgccc
      241 acggacaacg gcacagaggc tcccactgcc cctgctcctg gacgtgctct gccagccagg
      301 aatcacggcc gcatgtggat gctgatcact gcagtgctcc tgtgctgcct ggtagctgtg
      361 ggtggtatcg ctgcatgggg gaagtccaaa gcaaaaagca ggtctgacat cttcagtctt
      421 gaaagcgcat ccaagggggg tggggtgagc aagggcgagg aggataacat ggccatcatc
      481 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      541 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      601 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
      661 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      721 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      781 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
      841 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
      901 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
      961 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     1021 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     1081 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     1141 ctgtacaag
//
