LOCUS       Exported                8776 bp DNA     circular SYN 02-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   eliasa55
  TITLE     Direct Submission
  JOURNAL   Exported Jun 20, 2022 from SnapGene 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8776
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      102..557
                     /label=M13 origin
     rep_origin      119..425
                     /label=F1 ori
     CDS             887..1546
                     /label=AmpR
     rep_origin      1644..2326
                     /label=ColE1 origin
     misc_feature    4528..4549
                     /label=148 Pph_seqF
     misc_feature    4612..5274
                     /label=GST
     primer_bind     5230..5249
                     /label=GST-Fw
     misc_feature    5275..5286
                     /label=GST
                     /label=GST(1)
     primer_bind     5301..5346
                     /label=Fragment 1.FOR
     primer_bind     5301..5346
                     /label=Fragment.FORa
     primer_bind     5301..5331
                     /label=Fragment.FOR
     misc_feature    5302..5322
                     /label=TEV cleavage ENLYFQ^G
     CDS             5329..6045
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     5982..6003
                     /label=EGFP-C_Fw
     primer_bind     complement(6025..6062)
                     /label=Fragment.REVa
     primer_bind     6045..6068
                     /label=Fragment.FOR
     misc_feature    6047..8236
                     /label=TBK1-CDS
     primer_bind     6638..6657
                     /label=TBK1 Seq Fw-1
     primer_bind     7257..7276
                     /label=TBK1 Seq Fw-2
     primer_bind     7477..7496
                     /label=TBK1 Seq Fw-3
     primer_bind     complement(8212..8260)
                     /label=Fragment.REV
     misc_feature    8271..8291
                     /label=4 SV40_seqR
     polyA_signal    complement(8305..8496)
                     /label=SV40 late polyA
     primer_bind     complement(8307..8338)
                     /label=SV40 Rv - SMP0004
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac
     4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga
     4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg
     4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
     4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
     4261 tcccccatct cccggtaccg catgctatgc atcagctgct agcaccatgg ctcgagatcc
     4321 cgggtgatca agtcttcgtc gagtgattgt aaataaaatg taatttacag tatagtattt
     4381 taattaatat acaaatgatt tgataataat tcttatttaa ctataatata ttgtgttggg
     4441 ttgaattaaa ggtccgtata ctccggaata ttaatagatc atggagataa ttaaaatgat
     4501 aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa
     4561 cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc catggcatcc
     4621 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa
     4681 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga
     4741 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat
     4801 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg
     4861 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt
     4921 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt
     4981 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat
     5041 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt
     5101 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt
     5161 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct
     5221 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt
     5281 ccgcgtggat caggatcagg agagaattta tactttcagg gagcgcgcat ggtgagcaag
     5341 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     5401 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     5461 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     5521 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     5581 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     5641 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     5701 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     5761 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     5821 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     5881 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     5941 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     6001 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagcatgc aatccacatc
     6061 caaccacctc tggctcctct cggacattct cggacaaggc gctaccgcta acgtgttcag
     6121 gggaaggcac aagaagaccg gtgacctgtt cgccatcaag gtgttcaaca acatctcatt
     6181 cctgcgccct gtggacgtcc agatgcgtga gttcgaagtg ctgaagaagc tgaaccacaa
     6241 gaacatcgtc aagctgttcg ccatcgagga agaaaccact acccgccaca aggtgctgat
     6301 catggagttc tgcccctgcg gttccctgta cactgtcctg gaagagcctt ccaacgctta
     6361 cggtctgccc gagagcgagt tcctgatcgt cctgcgtgac gtggtcggtg gcatgaacca
     6421 cctgagggaa aacggcatcg tgcacagaga catcaagcca ggaaacatca tgcgcgtgat
     6481 cggcgaggac ggacagagcg tctacaagct gaccgacttc ggcgctgcca gagagctgga
     6541 agacgacgaa cagttcgtct ctctgtacgg aactgaagag tacctgcacc ctgacatgta
     6601 cgagagggcc gtgctgagaa aggaccacca gaagaagtac ggtgctaccg tggacctgtg
     6661 gtctatcggc gtcaccttct accacgctgc cactggatca ctgcctttcc gccccttcga
     6721 aggtcctcgc cgtaacaagg aggtcatgta caagatcatc actggcaagc cctctggcgc
     6781 catctcagga gtccagaagg ctgaaaacgg tcccatcgac tggagcggcg acatgccagt
     6841 gagctgctct ctgtcccgcg gtctgcaagt gctgctgacc ccagtcctgg ccaacatcct
     6901 ggaagctgac caggagaagt gctggggttt cgaccagttc ttcgctgaaa cctccgacat
     6961 cctgcacaga atggtcatcc acgtcttcag cctgcaacag atgactgccc acaagattta
     7021 catccactct tacaacactg ctactatctt ccacgaactg gtctacaagc agaccaagat
     7081 catctccagc aaccaggaac tgatctacga gggaaggaga ctggtgctgg agcccggtcg
     7141 cctggctcag cacttcccaa agactaccga agagaaccct atcttcgtgg tgtcccgcga
     7201 gccactgaac actatcggcc tgatctacga gaagatcagc ctgcccaagg tccacccacg
     7261 ttacgacctg gacggcgacg ctagcatggc taaggccatc accggagtgg tctgctacgc
     7321 ttgccgcatc gcttcaactc tgctgctgta ccaggaactg atgcgcaagg gaatccgttg
     7381 gctgatcgag ctgatcaagg acgactacaa cgaaactgtg cacaagaaga ccgaggtggt
     7441 catcactctg gacttctgca tccgtaacat cgaaaagacc gtgaaggtct acgagaagct
     7501 gatgaagatc aacctggagg ctgccgaact gggagagatc agcgacatcc acactaagct
     7561 gctgcgcctg tcttcatccc agggcactat cgaaacctcc ctgcaagaca tcgactcccg
     7621 tctgagccca ggaggttcac tggctgacgc ttgggctcac caggaaggca cccaccctaa
     7681 ggacaggaac gtggagaagc tgcaagtcct gctgaactgc atgactgaaa tctactacca
     7741 gttcaagaag gacaaggccg agcgccgtct ggcttacaac gaagagcaga tccacaagtt
     7801 cgacaagcag aagctgtact accacgccac caaggctatg actcacttca ccgacgaatg
     7861 cgtcaagaag tacgaggctt tcctgaacaa gagcgaagag tggatcagga agatgctgca
     7921 cctgagaaag cagctgctgt ccctgaccaa ccagtgcttc gacatcgaag aggaagtgag
     7981 caagtaccag gagtacacca acgagctgca agagactctg cctcagaaga tgttcactgc
     8041 cagctctggt atcaagcaca ctatgacccc aatctaccct tcatccaaca ccctggtgga
     8101 aatgactctg ggtatgaaga agctgaagga ggaaatggag ggcgtggtca aggaactggc
     8161 tgagaacaac cacatcttgg aaagattcgg tagcctgacg atggatggtg gactgagaaa
     8221 cgtggactgt ctgtaaaagc ttgtcgagaa gtactagagg atcataatca gccataccac
     8281 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     8341 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     8401 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8461 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag
     8521 gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg
     8581 tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc
     8641 cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg
     8701 catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt gacaaaccgt
     8761 catcttcggc tacttt
//
