LOCUS       pEcoFus_(pFP41)         5555 bp ds-DNA     circular     10-MAY-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter              2320..2420
                           /label="4.5S RNA promoter"
                           /ApEinfo_revcolor=#b1ff67
                           /ApEinfo_fwdcolor=#b1ff67
     terminator            2454..2484
                           /label="terminator"
                           /ApEinfo_revcolor=#c6c9d1
                           /ApEinfo_fwdcolor=#c6c9d1
     CDS                   complement(2782..3153)
                           /label="traJ"
                           /ApEinfo_revcolor=#993366
                           /ApEinfo_fwdcolor=#993366
     oriT                  3186..3295
                           /label="oriT"
                           /ApEinfo_revcolor=#b4abac
                           /ApEinfo_fwdcolor=#b4abac
     CDS                   complement(3671..4294)
                           /label="catP"
                           /ApEinfo_revcolor=#993366
                           /ApEinfo_fwdcolor=#993366
     rep_origin            complement(4655..5243)
                           /label="colE"
                           /ApEinfo_revcolor=#ffef86
                           /ApEinfo_fwdcolor=#ffef86
     origin of replication 5552..1969
                           /label="Fuso ORI"
                           /ApEinfo_revcolor=#ff9ccd
                           /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 AGATATTCTT TTTTAAACTT TTCAAATGCT TCAAATTCTA CATCTGCAAC TTCTACATCT
       61 TTTCCAGTAT TTCCAAACTT CACACAATTT TTGTAAACAT TTCTTAATTT TTCCAAGTAT
      121 TCTATGTCTT CCTTTTCTTC TTTAACATAC TCTTGTAACT GTGTCATCAA ATTAGTTTTG
      181 TCTTCCACAT TCCCCCTTTT TCTTGTAATT GCCTTATTTA ATTCTTCAAA TCTTATTGTT
      241 TTCATTTTAT TGTACCTCCA ATTTTTTTAT ATATCTATGT TAAAAAACAT ATTGACAATT
      301 GATTAAAACA ATGATATTAT GGTCGGTTGA GCTCATTGAG CTGAGTGCAG ACTTTCCCCC
      361 TTGTAAAAAG GGGAAAAAAC GATTGATAAT GATACAATGG TCGTATATCG CTATGTTCGC
      421 ATCGTTTAAC TTACTTTATC GCATATTTAA TTATATGCCA GTGTTAGCCC TGCCCAACAC
      481 CTAGAGTTTT AGCTTACTTT CTAAATTATA ACCCTAATAA AGTACCCACT CAGAGCACCT
      541 CCAAGACTTT TAAAGGTAGC CTCGCACCTT GCTACAATTC ATAATACCAC CATTACGGTG
      601 CTATACCGTT TCGAGCAGAA CGGCTATACA TACGAATTTA TCTTATGTAT AAAATTTGTT
      661 ACAAATATAA TAGTAAGTTT TTTTTATATA AAAAAGCAGA TCGCTTTTAT TTTTTTTGCA
      721 ACCTGCTTCA CAACTCATTT TTTTACTTTA GTAAATAAAT CCTTTACATC TTTTAAAAAA
      781 TATGGTATAT TAAGTCAAAG CAGGCATAAC ATATATGTTA TGTTTTATAG TAAAAATGGA
      841 CTATTCAACT ATTTAAAAAA TGGTAGAATA CAAATAATTT TCCTGGCAGT CAATTATTTT
      901 TTAAAAATTT TTACTATTAT ATTTTTAAAT TTTAAATTTT AGATAGAGTT ATTTGGCGAT
      961 AACTCTTTTT GTCTTACTTC TGAGTTTAGC ATATTTTTTT TGTTTTGTAA ATCCATTTCT
     1021 TTATTTTTCT TTACAACATA AACTTTTTTT ACTTCTCTAT AAATCTTCTC ACCAGTTGAA
     1081 ACATCAATTT CTAACAGTTC TCTAGCAATA AAATTTTTAA TATAGAGTAA ATCTTTTTCA
     1141 TCTGTAAGTA TATCATTCAT TTCATCAAAA TCCAACTTTT TTTTGGTTTC TTTTGGTTTT
     1201 ATTAAATTTC GTGAACAGAA ATAGGCTTTT TTTTGATTTG CTTTCTTTTC TAAGTCTTTC
     1261 ACAAAATACT TCGTTATATA CACCCCCACA GCTTCATTTT TAACAGTTTC TTTAATCTTA
     1321 TTTATTTTTA TAAATCCATT CGCCCACAAT TCCCCAAGCT TCTTATTATC TATATATGGG
     1381 ACCGAGAACA GCACCAAATG ATAATGGATT GCTCCCCTCT GCTGGAGTTC CCAAGTTGCA
     1441 ATATATTTAA GCTGTTGATT TTTCAAGTAC CTTTTCAATC TCTTAATAAA TAAAGTAAAT
     1501 TCACGATTTG CCCTTTCTAT ATCCTGTATA TTTTCTTTAA AAGTAAGAGT TAAAAAGGTA
     1561 CTTTTATCAT CATAATTACA ATCAATTATC CTTTTTATTT CAAATCGCTT GTCCTGGTAA
     1621 TATGCTTTCC TTCGTTGCAT ACTTTCAGCT TTTTCAATGC TACTTAATTC TTCAAAATCT
     1681 CTTCTTTTTC TTCCTGGTCT TCTTTTAAAG TCAAAAGCAG TTAAAGCATC AATATTGAAA
     1741 ATGTATTCAG TTATATTTTT TGATTTTATT TCTTTAGTGT TATAAAATTT CAACTCAATA
     1801 AATTCCCCCT TTATTAATCT GCATAATCTT TTTTATAATG TCCTTTACAA CCTAATATTC
     1861 TTAATCTATC ATTTTTTAAG TCATAAATTA GTCTATGTTC ATCACTTATT CTTCTGCTAT
     1921 AAATAGCCCT ATTTTTAAAA TGTTTTAATC TCTCAGGCTT ACCAGTACCg cggccgcCTC
     1981 CCAACATAAA CATATATTGC TTGTTCTAAC TCATATTTTT GTAAAATTTC AACATCATAT
     2041 CCAGTCAAAT CGTACTCTCT CTTATATACA AATGGTTTTT CTAATTTTTT GTTCAATAAA
     2101 TCTCCTCCTA TTTCTTACTT AATTTAGCTA AAAATCTATT TATATTTCAC CTCCTTTTTG
     2161 ACTTTAAGCC TACGAATACC ATATTTATGA TTATAACATC TTCTATTTTT CCCAAATCCT
     2221 TACATCTCCC CTAATTTTTA GACTTAAGGG CGAATTCTGC ATCAAGCTAG CATAAAAATA
     2281 AGAAGCCTGC ATTTGCAGGC TTCTTATTTT TATGgtacct taaaaacaaa gataagagaa
     2341 agaataaaaa gtgattttct taaaagaata gcgggttaat ataaagttga caaaaaagaa
     2401 taaaaatgat aatatatatt gGATCCTATA AGTTTTAATA AAACTTTAAA TAGAAAAAGG
     2461 CTTCTCTCAT GAGAAGTCTT TTTTATTTAA AATAAATATA AAATAAAATA GAGGCTATAA
     2521 ATAGCCTCTA TTTTATGTGA GAAATCCCTA AATAAAAAGA TGCCAGTGTG CTGGAATTCC
     2581 CCTTCAATAT TCCACAATAA GGCTGTAGGC GCTAGGGACC TCTTTAGCTC CTTGGAAGCT
     2641 GTCAGTAGTA GGGGATCGGT CTTGCCTTGC TCGTCGGTGA TGTACTTCAC CAGCTCCGCG
     2701 AAGTCGCTCT TCTTGATGGA GCGCATGGGG ACGTGCTTGG CAATCACGCG CACCCCCCGG
     2761 CCGTTTTAGC GGCTAAAAAA GTCATGGCTC TGCCCTCGGG CGGACCACGC CCATCATGAC
     2821 CTTGCCAAGC TCGTCCTGCT TCTCTTCGAT CTTCGCCAGC AGGGCGAGGA TCGTGGCATC
     2881 ACCGAACCGC GCCGTGCGCG GGTCGTCGGT GAGCCAGAGT TTCAGCAGGC CGCCCAGGCG
     2941 GCCCAGGTCG CCATTGATGC GGGCCAGCTC GCGGACGTGC TCATAGTCCA CGACGCCCGT
     3001 GATTTTGTAG CCCTGGCCGA CGGCCAGCAG GTAGGCCGAC AGGCTCATGC CGGCCGCCGC
     3061 CGCCTTTTCC TCAATCGCTC TTCGTTCGTC TGGAAGGCAG TACACCTTGA TAGGTGGGCT
     3121 GCCCTTCCTG GTTGGCTTGG TTTCATCAGC CATCCGCTTG CCCTCATCTG TTACGCCGGC
     3181 GGTAGCCGGC CAGCCTCGCA GAGCAGGATT CCCGTTGAGC ACCGCCAGGT GCGAATAAGG
     3241 GACAGTGAAG AAGGAACACC CGCTCGCGGG TGGGCCTACT TCACCTATCC TGCCCGGCTG
     3301 ACGCCGTTGG ATACACCAAG GAAAGTCTAC ACGAACCCTT TGGCAAAATC CTGTATATCG
     3361 TGCGAAAAAG GATGGATATA CCGAAAAAAT CGCTATAATG ACCCCGAAGC AGGAAGGGCG
     3421 AATTCTGCAG ATCTGACCGG TCTCTGAAAA TATAAAAACC ACAGATTGAT ACTAAAACCT
     3481 TGGTTGTGTT GCTTTTCGGG GCTTAAATCA AGGAAAAATC CTTGTTTTAA GCCTTTCAAA
     3541 AAGAAACACA AGGTCTTTGT ACTAACCTGT GGTTATGTAT AAAATTGTAG ATTTTAGGGT
     3601 AACAAAAAAC ACCGTATTTC TACGATGTTT TTGCTTAAAT ACTTGTTTTT AGTTACAGAC
     3661 AAACCTGAAG TTAACTATTT ATCAATTCCT GCAATTCGTT TACAAAACGG CAAATGTGAA
     3721 ATCCGTCACA TACTGCGTGA TGAACTTGAA TTGCCAAAGG AAGTATAATT TTGTTATCTT
     3781 CTTTATAATA TTTCCCCATA GTAAAAATAG GAATCAAATA ATCATATCCT TTCTGCAAAT
     3841 TCAGATTAAA GCCATCGAAG GTTGACCACG GTATCATAGA TACATTAAAA ATGTTTTCCG
     3901 GAGCATTTGG CTTTCCTTCC ATTCTATGAT TGTTTCCATA CCGTTGCGTA TCACTTTCAT
     3961 AATCTGCTAA AAATGATTTA AAGTCAGACT TACACTCAGT CCAAAGGCTG GAAAATGTTT
     4021 CAGTATCATT GTGAAATATT GTATAGCTTG GTATCATCTC ATCATATATC CCCAATTCAC
     4081 CATCTTGATT GATTGCCGTC CTAAACTCTG AATGGCGGTT TACAATCATT GCAATATAAT
     4141 AAAGCATTGC AGGATATAGT TTCATTCCCT TTTCCTTTAT TTGTGTGATA TCCACTTTAA
     4201 CGGTCATGCT GTAGGTACAA GGTACACTTG CAAAGTAGTG GTCAAAATAC TCTTTTCTGT
     4261 TCCAACTATT TTTATCAATT TTTTCAAATA CCATCTAAGT TCCCTCTCAA ATTCAAGTTT
     4321 ATCGCTCTAA TGAACAAAGA TATTATACCA CATTTTTGTG AATTTTTCAA CTTGCCCACT
     4381 TCGACTGCAC TCCCGACTTA ATAACTTCTT GAACACTTGC CGAAAAAGAA AAACTGCCGG
     4441 GTACGTACCC GGGATCGATC CCCGCCGAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
     4501 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT
     4561 TATCCACTGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG
     4621 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG
     4681 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT
     4741 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
     4801 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT
     4861 GTAGGTATCA CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC
     4921 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA
     4981 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG
     5041 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG
     5101 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT
     5161 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA
     5221 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC
     5281 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAA ATGCAAGTTT CTAACTAACA
     5341 TACATCATAG TTACTAAACT ATGGTAACTA TAATTTTATT AACTATATTA AGCACTGATT
     5401 AGTACTATAA CTCAATATAA GCATATCCCC TGTATCGTAA CTAGAGAACC AAACGACGGA
     5461 AAAAGCGATA TAGATAAGTT TAGATAGTGC ACCAATTTTT TACTTTTCTA TTTTAGAATC
     5521 TAGCATTTCC AATGCTTACT AATACcgatc gTTCT
//