LOCUS       pAAV-hSyn-eGFP-P        5850 bp    DNA     circular     25-Jun-2022
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     repeat_region     6..135
                     /label="AAV2 ITR (alternate)"
                     /note="AAV2 ITR (alternate)"
     repeat_region     3118..3247
                     /label="AAV2 ITR"
                     /note="AAV2 ITR"
     promoter        4070..4174
                     /label="AmpR promoter"
                     /note="AmpR promoter"
     CDS             4175..5035
                     /label="AmpR"
                     /note="AmpR"
     rep_origin      5206..5794
                     /label="ori"
                     /note="ori"
     rep_origin      3333..3788
                     /label="f1 ori"
                     /note="f1 ori"
     promoter        180..627
                     /label="hSyn promoter"
                     /note="hSyn promoter"
     polyA_signal     2602..3078
                     /label="hGH poly(A) signal"
                     /note="hGH poly(A) signal"
     misc_feature     1982..2570
                     /label="WPRE"
                     /note="WPRE"
     repeat_region     3118..3258
                     /label="AAV2 ITR"
                     /note="AAV2 ITR"
     CDS             complement(2453..2464)
                     /label="Factor Xa site"
                     /note="Factor Xa site"
     CDS             647..1969
                     /label="eGFP-P2A-LgBiT-Link-SV40NLS"
     misc_feature     647..1369
                     /label="eGFP"
     misc_feature     1370..1435
                     /label="P2A "
     misc_feature     1442..1915
                     /label="LgBiT"
     misc_feature     1916..1945
                     /label="Flexible linker"
     misc_feature     1946..1966
                     /label="SV40 NLS"
     misc_feature     574..591
                     /label="hSyn Left 1"
     misc_feature     1061..1080
                     /label="Found -> DNA"
                     /note="hSyn left 1/gfp f"
     misc_feature     complement(2038..2059)
                     /label="Found <- DNA"
                     /note="hSyn Right 2"
ORIGIN
        1 catgtcctgc aggcagctgc gcgctcgctc gctcactgag gccgcccggg cgtcgggcga 
       61 cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca 
      121 tcactagggg ttcctgcggc cgcacgcgtg tgtctagact gcagagggcc ctgcgtatga 
      181 gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac 
      241 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag 
      301 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg 
      361 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg 
      421 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc 
      481 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc 
      541 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga 
      601 ggagtcgtgt cgtgcctgag agcgcagtcg agaaggtacc gccaccatgg tgagcaaggg 
      661 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 
      721 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 
      781 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 
      841 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 
      901 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 
      961 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 
     1021 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 
     1081 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 
     1141 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 
     1201 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 
     1261 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 
     1321 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aaggaattcg gaagcggagc 
     1381 tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc 
     1441 cgtcttcaca ctcgaagatt tcgttgggga ctgggaacag acagccgcct acaacctgga 
     1501 ccaagtcctt gaacagggag gtgtgtccag tttgctgcag aatctcgccg tgtccgtaac 
     1561 tccgatccaa aggattgtcc ggagcggtga aaatgccctg aagatcgaca tccatgtcat 
     1621 catcccgtat gaaggtctga gcgccgacca aatggcccag atcgaagagg tgtttaaggt 
     1681 ggtgtaccct gtggatgatc atcactttaa ggtgatcctg ccctatggca cactggtaat 
     1741 cgacggggtt acgccgaaca tgctgaacta tttcggacgg ccgtatgaag gcatcgccgt 
     1801 gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga 
     1861 cgagcgcctg atcacccccg acggctccat gctgttccga gtaaccatca acagcggagg 
     1921 cggaggctca ggaggcggag gctcaccaaa gaagaagcgt aaggtataaa agcttatcga 
     1981 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 
     2041 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 
     2101 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 
     2161 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 
     2221 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 
     2281 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 
     2341 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct 
     2401 cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 
     2461 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 
     2521 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg 
     2581 agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg 
     2641 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc 
     2701 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc 
     2761 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg 
     2821 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc 
     2881 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt 
     2941 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa 
     3001 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact 
     3061 gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg 
     3121 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg 
     3181 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag 
     3241 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta 
     3301 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc 
     3361 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc 
     3421 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 
     3481 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 
     3541 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 
     3601 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 
     3661 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg 
     3721 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt 
     3781 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc 
     3841 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc 
     3901 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc 
     3961 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 
     4021 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 
     4081 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 
     4141 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 
     4201 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 
     4261 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 
     4321 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 
     4381 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact 
     4441 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 
     4501 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 
     4561 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 
     4621 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 
     4681 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg 
     4741 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 
     4801 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 
     4861 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 
     4921 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 
     4981 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 
     5041 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 
     5101 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 
     5161 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 
     5221 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 
     5281 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 
     5341 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 
     5401 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 
     5461 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 
     5521 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 
     5581 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 
     5641 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 
     5701 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 
     5761 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 
     5821 gttcctggcc ttttgctggc cttttgctca 
//