LOCUS       pRK172-6XHis-0N4        3760 bp    DNA     circular     15-Jun-2022
SOURCE
  ORGANISM
COMMENT     This file was created by DNADynamo
COMMENT     www.bluetractorsoftware.co.uk
FEATURES             Location/Qualifiers
     Promoter        140..168
                     /label="Promoter ampicillin_resistance_gene(b-lactamase)promoter"
                     /note="Pr_amp"
     Selection       210..1070
                     /label="Selection ampicillin_resistance_gene_ORF"
                     /note="amp"
     Origin          1225..1844
                     /label="Origin pBR322_replication_origin"
                     /note="Ori_pBR322"
     Other           2371..2387
                     /label="Other T7_gene_10_translational_enhancer_RBS"
                     /note="T7_transl_en_RBS"
     Terminator      3631..3758
                     /label="Terminator T7_transcription_termination_region"
                     /note="T7"
     CDS             2399..3616
                     /label="6XHis Tau HiBiT"
     Insert          2399..3616
                     /label="Insert Insert from feature 6XHis Tau HiBiT (from His Tau HiBiT PCR Product)"
                     /note="Insert from feature 6XHis Tau HiBiT (from His Tau HiBiT PCR Product)"
ORIGIN
        1 nttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 
       61 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 
      121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 
      181 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 
      241 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 
      301 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 
      361 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 
      421 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc 
      481 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 
      541 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 
      601 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 
      661 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 
      721 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac 
      781 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 
      841 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 
      901 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 
      961 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 
     1021 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 
     1081 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 
     1141 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 
     1201 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 
     1261 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 
     1321 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 
     1381 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 
     1441 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 
     1501 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 
     1561 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 
     1621 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 
     1681 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 
     1741 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 
     1801 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 
     1861 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 
     1921 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 
     1981 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc 
     2041 atctgtgcgg tatttcacac cgcagatggt gcactctcag tacaatctgc tctgatgccg 
     2101 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg 
     2161 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 
     2221 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 
     2281 gaaacgcgcg aggcaggatc tcgatcccgc gaaattaata cgatacacta tagggagacc 
     2341 acaacggttt ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgat 
     2401 gatgcatcac catcaccatc acgctgagcc ccgccaggag ttcgaagtga tggaagatca 
     2461 cgctgggacg tacgggttgg gggacaggaa agatcagggg ggctacacca tgcaccaaga 
     2521 ccaagagggt gacacggacg ctggcctgaa agctgaagaa gcaggcattg gagacacccc 
     2581 cagcctggaa gacgaagctg ctggtcacgt gacccaagct cgcatggtca gtaaaagcaa 
     2641 agacgggact ggaagcgatg acaaaaaagc caagggggct gatggtaaaa cgaagatcgc 
     2701 cacaccgcgg ggagcagccc ctccaggcca gaagggccag gccaacgcca ccaggattcc 
     2761 agcaaaaacc ccgcccgctc caaagacacc acccagctct ggtgaacctc caaaatcagg 
     2821 ggatcgcagc ggctacagca gccccggctc cccaggcact cccggcagcc gctcccgcac 
     2881 cccgtccctt ccaaccccac ccacccggga gcccaagaag gtggcagtgg tccgtactcc 
     2941 acccaagtcg ccgtcttccg ccaagagccg cctgcagaca gcccccgtgc ccatgccaga 
     3001 cctgaagaat gtcaagtcca agatcggctc cactgagaac ctgaagcacc agccgggagg 
     3061 cgggaaggtg cagataatta ataagaagct ggatcttagc aacgtccagt ccaagtgtgg 
     3121 ctcaaaggat aatatcaaac acgtcagtgg aggcggcagt gtgcaaatag tctacaaacc 
     3181 agttgacctg agcaaggtga cctccaagtg tggctcatta ggcaacatcc atcataaacc 
     3241 aggaggtggc caggtggaag taaaatctga gaagcttgac ttcaaggaca gagtccagtc 
     3301 gaagattggg tccctggaca atatcaccca cgtccctggc ggaggaaata aaaagattga 
     3361 aacccacaag ctgaccttcc gcgagaacgc caaagccaag acagaccacg gggcggagat 
     3421 cgtgtacaag tcgccagtgg tgtctgggga cacgtctcca cggcatctca gcaatgtctc 
     3481 ctccaccggc agcatcgaca tggtagactc gccccagctc gccacgctag ctgacgaggt 
     3541 gtctgcctcc ctggccaagc agggtttggg ttcaggttca gtgagcggct ggcgcctgtt 
     3601 caagaagatc agctaggaat tccgatccgg ctgctaacaa agcccgaaag gaagctgagt 
     3661 gggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct 
     3721 tgaggggttt tttgctgaaa ggaggaacta tatccgataa 
//