LOCUS       pVoPo-03                4561 bp ds-DNA     circular     15-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    30..56
                     /label="Col-for (JVO-18406)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    257..275
                     /label="tet operator"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    286..286
                     /label="TSS is 1nt downstream"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      320..350
                     /label="slpA terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     ORI             430..1112
                     /label="colE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(443..468)
                     /label="Col-rev (JVO-18400)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1048..1076
                     /label="JVO-18401"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1233..1856)
                     /label="catP"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(1356..1384)
                     /label="JVO-18402"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1775..1803
                     /label="JVO-18403"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     ORI             1960..3942
                     /label="Fuso-ORI"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(1995..2023)
                     /label="JV-18404"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3869..3896
                     /label="JVO-18405"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(3978..3997)
                     /label="OPEN-for (JVO-18370)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3998..4016
                     /label="OPEN-rev (JVO-18369)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(4085..147)
                     /label="TetR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    complement(4142..4161)
                     /label="Col-rev2 (JVO-19951)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
       61 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat
      121 cactttactt ttatctaatc tagacatcat taattcctcc tttttgttga cattatatca
      181 ttgatagagt tatttgtcaa actagttttt tatttcgatg ccctggactt catgaaaaac
      241 taaaaaaaat attgacactc tatcattgat agagtataat taaaatggat cctataagtt
      301 ttaataaaac tttaaataga aaaaggcttc tctcatgaga agtctttttt atttaaaata
      361 aatataaaat aaaatagagg ctataaatag cctctatttt atgtgagaaa tccctaaata
      421 aaaaactagt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
      481 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
      541 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
      601 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
      661 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
      721 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
      781 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
      841 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt
      901 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
      961 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1021 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1081 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1141 gaattcttat ggattttagg gtaacaaaaa acaccgtatt tctacgatgt ttttgcttaa
     1201 atacttgttt ttagttacag acaaacctga agttaactat ttatcaattc ctgcaattcg
     1261 tttacaaaac ggcaaatgtg aaatccgtca catactgcgt gatgaacttg aattgccaaa
     1321 ggaagtataa ttttgttatc ttctttataa tatttcccca tagtaaaaat aggaatcaaa
     1381 taatcatatc ctttctgcaa attcagatta aagccatcga aggttgacca cggtatcata
     1441 gatacattaa aaatgttttc cggagcattt ggctttcctt ccattctatg attgtttcca
     1501 taccgttgcg tatcactttc ataatctgct aaaaatgatt taaagtcaga cttacactca
     1561 gtccaaaggc tggaaaatgt ttcagtatca ttgtgaaata ttgtatagct tggtatcatc
     1621 tcatcatata tccccaattc accatcttga ttgattgccg tcctaaactc tgaatggcgg
     1681 tttacaatca ttgcaatata ataaagcatt gcaggatata gtttcattcc cttttccttt
     1741 atttgtgtga tatccacttt aacggtcatg ctgtaggtac aaggtacact tgcaaagtag
     1801 tggtcaaaat actcttttct gttccaacta tttttatcaa ttttttcaaa taccatttaa
     1861 aattacctcc ttaaaatata ctgctttaat tgtataattt tagctaatta ttgtcaagta
     1921 tttttaaagt tatgtaaatt ttttacttat attttatcga tcgTTCTAGA TATTCTTTTT
     1981 TAAACTTTTC AAATGCTTCA AATTCTACAT CTGCAACTTC TACATCTTTT CCAGTATTTC
     2041 CAAACTTCAC ACAATTTTTG TAAACATTTC TTAATTTTTC CAAGTATTCT ATGTCTTCCT
     2101 TTTCTTCTTT AACATACTCT TGTAACTGTG TCATCAAATT AGTTTTGTCT TCCACATTCC
     2161 CCCTTTTTCT TGTAATTGCC TTATTTAATT CTTCAAATCT TATTGTTTTC ATTTTATTGT
     2221 ACCTCCAATT TTTTTATATA TCTATGTTAA AAAACATATT GACAATTGAT TAAAACAATG
     2281 ATATTATGGT CGGTTGAGCT CATTGAGCTG AGTGCAGACT TTCCCCCTTG TAAAAAGGGG
     2341 AAAAAACGAT TGATAATGAT ACAATGGTCG TATATCGCTA TGTTCGCATC GTTTAACTTA
     2401 CTTTATCGCA TATTTAATTA TATGCCAGTG TTAGCCCTGC CCAACACCTA GAGTTTTAGC
     2461 TTACTTTCTA AATTATAACC CTAATAAAGT ACCCACTCAG AGCACCTCCA AGACTTTTAA
     2521 AGGTAGCCTC GCACCTTGCT ACAATTCATA ATACCACCAT TACGGTGCTA TACCGTTTCG
     2581 AGCAGAACGG CTATACATAC GAATTTATCT TATGTATAAA ATTTGTTACA AATATAATAG
     2641 TAAGTTTTTT TTATATAAAA AAGCAGATCG CTTTTATTTT TTTTGCAACC TGCTTCACAA
     2701 CTCATTTTTT TACTTTAGTA AATAAATCCT TTACATCTTT TAAAAAATAT GGTATATTAA
     2761 GTCAAAGCAG GCATAACATA TATGTTATGT TTTATAGTAA AAATGGACTA TTCAACTATT
     2821 TAAAAAATGG TAGAATACAA ATAATTTTCC TGGCAGTCAA TTATTTTTTA AAAATTTTTA
     2881 CTATTATATT TTTAAATTTT AAATTTTAGA TAGAGTTATT TGGCGATAAC TCTTTTTGTC
     2941 TTACTTCTGA GTTTAGCATA TTTTTTTTGT TTTGTAAATC CATTTCTTTA TTTTTCTTTA
     3001 CAACATAAAC TTTTTTTACT TCTCTATAAA TCTTCTCACC AGTTGAAACA TCAATTTCTA
     3061 ACAGTTCTCT AGCAATAAAA TTTTTAATAT AGAGTAAATC TTTTTCATCT GTAAGTATAT
     3121 CATTCATTTC ATCAAAATCC AACTTTTTTT TGGTTTCTTT TGGTTTTATT AAATTTCGTG
     3181 AACAGAAATA GGCTTTTTTT TGATTTGCTT TCTTTTCTAA GTCTTTCACA AAATACTTCG
     3241 TTATATACAC CCCCACAGCT TCATTTTTAA CAGTTTCTTT AATCTTATTT ATTTTTATAA
     3301 ATCCATTCGC CCACAATTCC CCAAGCTTCT TATTATCTAT ATATGGGACC GAGAACAGCA
     3361 CCAAATGATA ATGGATTGCT CCCCTCTGCT GGAGTTCCCA AGTTGCAATA TATTTAAGCT
     3421 GTTGATTTTT CAAGTACCTT TTCAATCTCT TAATAAATAA AGTAAATTCA CGATTTGCCC
     3481 TTTCTATATC CTGTATATTT TCTTTAAAAG TAAGAGTTAA AAAGGTACTT TTATCATCAT
     3541 AATTACAATC AATTATCCTT TTTATTTCAA ATCGCTTGTC CTGGTAATAT GCTTTCCTTC
     3601 GTTGCATACT TTCAGCTTTT TCAATGCTAC TTAATTCTTC AAAATCTCTT CTTTTTCTTC
     3661 CTGGTCTTCT TTTAAAGTCA AAAGCAGTTA AAGCATCAAT ATTGAAAATG TATTCAGTTA
     3721 TATTTTTTGA TTTTATTTCT TTAGTGTTAT AAAATTTCAA CTCAATAAAT TCCCCCTTTA
     3781 TTAATCTGCA TAATCTTTTT TATAATGTCC TTTACAACCT AATATTCTTA ATCTATCATT
     3841 TTTTAAGTCA TAAATTAGTC TATGTTCATC ACTTATTCTT CTGCTATAAA TAGCCCTATT
     3901 TTTAAAATGT TTTAATCTCT CAGGCTTACC AGTACCgcgg ccgctccagt aatgacctca
     3961 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
     4021 aatccaagca ctagtcataa aaataagaag cctgcatttg caggcttctt atttttatgg
     4081 taccttaaga cccactttca catttaagtt gtttttctaa tccgcatatg atcaattcaa
     4141 ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa taattcgata gcttgtcgta
     4201 ataatggcgg catactatca gtagtaggtg tttccctttc ttctttagcg acttgatgct
     4261 cttgatcttc caatacgcaa cctaaagtaa aatgccccac agcgctgagt gcatataatg
     4321 cattctctag tgaaaaacct tgttggcata aaaaggctaa ttgattttcg agagtttcat
     4381 actgtttttc tgtaggccgt gtacctaaat gtacttttgc tccatcgcga tgacttagta
     4441 aagcacatct aaaactttta gcgttattac gtaaaaaatc ttgccagctt tccccttcta
     4501 aagggcaaaa gtgagtatgg tgcctatcta acatctcaat ggctaaggcg tcgagcaaag
     4561 c
//