LOCUS       pVoPo-GFP               4541 bp ds-DNA     circular     15-JUN-2022
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     misc_feature    250..277
                     /label="JVO-18405"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(351..372)
                     /label="JVO-18158"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    353..372
                     /label="JVO-18156"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(359..378)
                     /label="JVO-18370"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    379..397
                     /label="JVO-18369"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(396..478)
                     /label="gene-specific primer: ACTAGTGCTTGGATTCTCACC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     404..491
                     /label="gene-specific primer: GTATATTAGAAAGGAGGAATATATAATGGTATC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    417..466
                     /label="promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     None            467..491
                     /label="5UTR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    492..1208
                     /label="sfGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      1242..1272
                     /label="slpA terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1303..1325)
                     /label="JVO-18155"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     ori             1352..2034
                     /label="ColE1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1365..1390)
                     /label="JVO-18400"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(1394..1417)
                     /label="JVO-18157"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1970..1998
                     /label="JVO-18401"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2010..2029
                     /label="JVO-18159"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     cds             complement(2155..2778)
                     /label="catP"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2208..2230)
                     /label="JVO-18160"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2278..2306)
                     /label="JVO-18402"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2697..2725
                     /label="JVO-18403"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2769..2805
                     /label="JVO-18073"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     ORI             2882..323
                     /label="ext. F-ORI"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2917..2945)
                     /label="JV-18404"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 ttcaatgcta cttaattctt caaaatctct tctttttctt cctggtcttc ttttaaagtc
       61 aaaagcagtt aaagcatcaa tattgaaaat gtattcagtt atattttttg attttatttc
      121 tttagtgtta taaaatttca actcaataaa ttcccccttt attaatctgc ataatctttt
      181 ttataatgtc ctttacaacc taatattctt aatctatcat tttttaagtc ataaattagt
      241 ctatgttcat cacttattct tctgctataa atagccctat ttttaaaatg ttttaatctc
      301 tcaggcttac cagtaccgcg gccgctccag taatgacctc agaactccat ctggatttgt
      361 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc actagtAAGC
      421 ATAAAAAAAA GTTGGATAAT ATACTAAAAT AGTGTATAAT AAATATgtat attagaaagg
      481 aggaatatat aATGAGAAAA GGAGAAGAAC TTTTTACAGG TGTTGTACCA ATATTAGTTG
      541 AATTAGATGG AGATGTTAAT GGTCATAAAT TTTCAGTTAG AGGAGAAGGA GAAGGTGATG
      601 CAACTAATGG AAAATTAACT CTTAAGTTTA TATGTACAAC AGGAAAATTA CCTGTACCAT
      661 GGCCTACTTT AGTAACTACA CTAACATATG GAGTTCAATG TTTTGCTAGA TATCCAGATC
      721 ATATGAAACA ACATGACTTT TTCAAATCTG CAATGCCAGA AGGATATGTT CAAGAAAGAA
      781 CAATAAGTTT TAAAGATGAT GGTACATATA AAACTAGAGC AGAAGTTAAA TTTGAAGGAG
      841 ATACTTTAGT TAATAGAATA GAGTTAAAAG GAATTGACTT TAAAGAAGAT GGAAATATTT
      901 TAGGACATAA ACTTGAGTAT AACTTTAATT CTCATAATGT TTACATTACT GCTGATAAAC
      961 AAAAGAATGG TATAAAAGCT AATTTCAAGA TAAGACATAA TGTAGAAGAT GGTTCTGTAC
     1021 AATTAGCAGA TCATTATCAG CAAAATACAC CTATTGGAGA TGGTCCTGTA TTATTACCAG
     1081 ATAATCACTA TCTTTCAACT CAATCAGTAC TAAGTAAAGA TCCTAATGAA AAAAGAGATC
     1141 ATATGGTTTT GTTAGAATTT GTTACAGCTG CAGGAATTAC TCATGGAATG GATGAATTGT
     1201 ATAAATAAgg atcctataag ttttaataaa actttaaata gaaaaaggct tctctcatga
     1261 gaagtctttt ttatttaaaa taaatataaa ataaaataga ggctataaat agcctctatt
     1321 ttatgtgaga aatccctaaa taaaaaacta gtcatgacca aaatccctta acgtgagttt
     1381 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1441 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1501 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1561 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1621 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1681 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1741 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1801 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1861 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1921 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1981 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2041 cggttcctgg ccttttgctg gcgaattctt atggatttta gggtaacaaa aaacaccgta
     2101 tttctacgat gtttttgctt aaatacttgt ttttagttac agacaaacct gaagttaact
     2161 atttatcaat tcctgcaatt cgtttacaaa acggcaaatg tgaaatccgt cacatactgc
     2221 gtgatgaact tgaattgcca aaggaagtat aattttgtta tcttctttat aatatttccc
     2281 catagtaaaa ataggaatca aataatcata tcctttctgc aaattcagat taaagccatc
     2341 gaaggttgac cacggtatca tagatacatt aaaaatgttt tccggagcat ttggctttcc
     2401 ttccattcta tgattgtttc cataccgttg cgtatcactt tcataatctg ctaaaaatga
     2461 tttaaagtca gacttacact cagtccaaag gctggaaaat gtttcagtat cattgtgaaa
     2521 tattgtatag cttggtatca tctcatcata tatccccaat tcaccatctt gattgattgc
     2581 cgtcctaaac tctgaatggc ggtttacaat cattgcaata taataaagca ttgcaggata
     2641 tagtttcatt cccttttcct ttatttgtgt gatatccact ttaacggtca tgctgtaggt
     2701 acaaggtaca cttgcaaagt agtggtcaaa atactctttt ctgttccaac tatttttatc
     2761 aattttttca aataccattt aaaattacct ccttaaaata tactgcttta attgtataat
     2821 tttagctaat tattgtcaag tatttttaaa gttatgtaaa ttttttactt atattttatc
     2881 gatcgttcta gatattcttt tttaaacttt tcaaatgctt caaattctac atctgcaact
     2941 tctacatctt ttccagtatt tccaaacttc acacaatttt tgtaaacatt tcttaatttt
     3001 tccaagtatt ctatgtcttc cttttcttct ttaacatact cttgtaactg tgtcatcaaa
     3061 ttagttttgt cttccacatt cccccttttt cttgtaattg ccttatttaa ttcttcaaat
     3121 cttattgttt tcattttatt gtacctccaa tttttttata tatctatgtt aaaaaacata
     3181 ttgacaattg attaaaacaa tgatattatg gtcggttgag ctcattgagc tgagtgcaga
     3241 ctttccccct tgtaaaaagg ggaaaaaacg attgataatg atacaatggt cgtatatcgc
     3301 tatgttcgca tcgtttaact tactttatcg catatttaat tatatgccag tgttagccct
     3361 gcccaacacc tagagtttta gcttactttc taaattataa ccctaataaa gtacccactc
     3421 agagcacctc caagactttt aaaggtagcc tcgcaccttg ctacaattca taataccacc
     3481 attacggtgc tataccgttt cgagcagaac ggctatacat acgaatttat cttatgtata
     3541 aaatttgtta caaatataat agtaagtttt ttttatataa aaaagcagat cgcttttatt
     3601 ttttttgcaa cctgcttcac aactcatttt tttactttag taaataaatc ctttacatct
     3661 tttaaaaaat atggtatatt aagtcaaagc aggcataaca tatatgttat gttttatagt
     3721 aaaaatggac tattcaacta tttaaaaaat ggtagaatac aaataatttt cctggcagtc
     3781 aattattttt taaaaatttt tactattata tttttaaatt ttaaatttta gatagagtta
     3841 tttggcgata actctttttg tcttacttct gagtttagca tatttttttt gttttgtaaa
     3901 tccatttctt tatttttctt tacaacataa acttttttta cttctctata aatcttctca
     3961 ccagttgaaa catcaatttc taacagttct ctagcaataa aatttttaat atagagtaaa
     4021 tctttttcat ctgtaagtat atcattcatt tcatcaaaat ccaacttttt tttggtttct
     4081 tttggtttta ttaaatttcg tgaacagaaa taggcttttt tttgatttgc tttcttttct
     4141 aagtctttca caaaatactt cgttatatac acccccacag cttcattttt aacagtttct
     4201 ttaatcttat ttatttttat aaatccattc gcccacaatt ccccaagctt cttattatct
     4261 atatatggga ccgagaacag caccaaatga taatggattg ctcccctctg ctggagttcc
     4321 caagttgcaa tatatttaag ctgttgattt ttcaagtacc ttttcaatct cttaataaat
     4381 aaagtaaatt cacgatttgc cctttctata tcctgtatat tttctttaaa agtaagagtt
     4441 aaaaaggtac ttttatcatc ataattacaa tcaattatcc tttttatttc aaatcgcttg
     4501 tcctggtaat atgctttcct tcgttgcata ctttcagctt t
//