LOCUS       pVoPo-mSc               4523 bp ds-DNA     circular     15-JUN-2022
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     misc_feature    250..277
                     /label="JVO-18405"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(351..372)
                     /label="JVO-18158"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    353..372
                     /label="JVO-18156"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(359..378)
                     /label="JVO-18370"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    379..397
                     /label="JVO-18369"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(396..478)
                     /label="gene-specific primer: ACTAGTGCTTGGATTCTCACC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     404..499
                     /label="gene-specific primer: GTATATTAGAAAGGAGGAATATATAATGGTATC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    417..466
                     /label="promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     None            467..491
                     /label="5UTR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    492..1190
                     /label="sfGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    492..1190
                     /label="mScarlet-I"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     terminator      1224..1254
                     /label="slpA terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1285..1307)
                     /label="JVO-18155"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     ori             1334..2016
                     /label="ColE1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1347..1372)
                     /label="JVO-18400"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(1376..1399)
                     /label="JVO-18157"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1952..1980
                     /label="JVO-18401"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1992..2011
                     /label="JVO-18159"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     cds             complement(2137..2760)
                     /label="catP"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2190..2212)
                     /label="JVO-18160"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2260..2288)
                     /label="JVO-18402"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2679..2707
                     /label="JVO-18403"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2751..2787
                     /label="JVO-18073"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     ORI             2864..323
                     /label="ext. F-ORI"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2899..2927)
                     /label="JV-18404"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 ttcaatgcta cttaattctt caaaatctct tctttttctt cctggtcttc ttttaaagtc
       61 aaaagcagtt aaagcatcaa tattgaaaat gtattcagtt atattttttg attttatttc
      121 tttagtgtta taaaatttca actcaataaa ttcccccttt attaatctgc ataatctttt
      181 ttataatgtc ctttacaacc taatattctt aatctatcat tttttaagtc ataaattagt
      241 ctatgttcat cacttattct tctgctataa atagccctat ttttaaaatg ttttaatctc
      301 tcaggcttac cagtaccgcg gccgctccag taatgacctc agaactccat ctggatttgt
      361 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc actagtAAGC
      421 ATAAAAAAAA GTTGGATAAT ATACTAAAAT AGTGTATAAT AAATATgtat attagaaagg
      481 aggaatatat aatggtatca aaaggtgaag cagttataaa agagtttatg agatttaaag
      541 ttcatatgga aggtagtatg aatggacatg aatttgagat tgaaggagaa ggagaaggta
      601 gaccttatga aggaacacaa acagctaaac ttaaagttac taaaggagga ccattaccat
      661 tttcttggga tattctttct cctcaattta tgtatggatc aagagctttt attaaacatc
      721 ctgcagatat acctgattat tataaacagt catttcctga aggatttaaa tgggaaagag
      781 ttatgaactt tgaagatgga ggagcagtta cagtaactca agatacttct ttagaagatg
      841 gaacattgat ttataaggta aaacttagag gaactaattt tccacctgat ggaccagtta
      901 tgcaaaagaa aacaatgggt tgggaagcat ctacagaaag attatatcca gaagatggag
      961 ttttaaaagg tgacataaaa atggctttaa gactaaaaga tggaggaaga tatttagcag
     1021 atttcaaaac tacatataaa gctaaaaaac cagtacaaat gcctggagct tataatgtag
     1081 atagaaagtt agacataact agtcacaatg aagattatac agttgttgaa caatatgaaa
     1141 gaagtgaagg tagacattca actggtggta tggatgaatt atacaaataa ggatcctata
     1201 agttttaata aaactttaaa tagaaaaagg cttctctcat gagaagtctt ttttatttaa
     1261 aataaatata aaataaaata gaggctataa atagcctcta ttttatgtga gaaatcccta
     1321 aataaaaaac tagtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1381 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1441 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1501 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     1561 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1621 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1681 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1741 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1801 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1861 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1921 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1981 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2041 tggcgaattc ttatggattt tagggtaaca aaaaacaccg tatttctacg atgtttttgc
     2101 ttaaatactt gtttttagtt acagacaaac ctgaagttaa ctatttatca attcctgcaa
     2161 ttcgtttaca aaacggcaaa tgtgaaatcc gtcacatact gcgtgatgaa cttgaattgc
     2221 caaaggaagt ataattttgt tatcttcttt ataatatttc cccatagtaa aaataggaat
     2281 caaataatca tatcctttct gcaaattcag attaaagcca tcgaaggttg accacggtat
     2341 catagataca ttaaaaatgt tttccggagc atttggcttt ccttccattc tatgattgtt
     2401 tccataccgt tgcgtatcac tttcataatc tgctaaaaat gatttaaagt cagacttaca
     2461 ctcagtccaa aggctggaaa atgtttcagt atcattgtga aatattgtat agcttggtat
     2521 catctcatca tatatcccca attcaccatc ttgattgatt gccgtcctaa actctgaatg
     2581 gcggtttaca atcattgcaa tataataaag cattgcagga tatagtttca ttcccttttc
     2641 ctttatttgt gtgatatcca ctttaacggt catgctgtag gtacaaggta cacttgcaaa
     2701 gtagtggtca aaatactctt ttctgttcca actattttta tcaatttttt caaataccat
     2761 ttaaaattac ctccttaaaa tatactgctt taattgtata attttagcta attattgtca
     2821 agtattttta aagttatgta aattttttac ttatatttta tcgatcgttc tagatattct
     2881 tttttaaact tttcaaatgc ttcaaattct acatctgcaa cttctacatc ttttccagta
     2941 tttccaaact tcacacaatt tttgtaaaca tttcttaatt tttccaagta ttctatgtct
     3001 tccttttctt ctttaacata ctcttgtaac tgtgtcatca aattagtttt gtcttccaca
     3061 ttcccccttt ttcttgtaat tgccttattt aattcttcaa atcttattgt tttcatttta
     3121 ttgtacctcc aattttttta tatatctatg ttaaaaaaca tattgacaat tgattaaaac
     3181 aatgatatta tggtcggttg agctcattga gctgagtgca gactttcccc cttgtaaaaa
     3241 ggggaaaaaa cgattgataa tgatacaatg gtcgtatatc gctatgttcg catcgtttaa
     3301 cttactttat cgcatattta attatatgcc agtgttagcc ctgcccaaca cctagagttt
     3361 tagcttactt tctaaattat aaccctaata aagtacccac tcagagcacc tccaagactt
     3421 ttaaaggtag cctcgcacct tgctacaatt cataatacca ccattacggt gctataccgt
     3481 ttcgagcaga acggctatac atacgaattt atcttatgta taaaatttgt tacaaatata
     3541 atagtaagtt ttttttatat aaaaaagcag atcgctttta ttttttttgc aacctgcttc
     3601 acaactcatt tttttacttt agtaaataaa tcctttacat cttttaaaaa atatggtata
     3661 ttaagtcaaa gcaggcataa catatatgtt atgttttata gtaaaaatgg actattcaac
     3721 tatttaaaaa atggtagaat acaaataatt ttcctggcag tcaattattt tttaaaaatt
     3781 tttactatta tatttttaaa ttttaaattt tagatagagt tatttggcga taactctttt
     3841 tgtcttactt ctgagtttag catatttttt ttgttttgta aatccatttc tttatttttc
     3901 tttacaacat aaactttttt tacttctcta taaatcttct caccagttga aacatcaatt
     3961 tctaacagtt ctctagcaat aaaattttta atatagagta aatctttttc atctgtaagt
     4021 atatcattca tttcatcaaa atccaacttt tttttggttt cttttggttt tattaaattt
     4081 cgtgaacaga aataggcttt tttttgattt gctttctttt ctaagtcttt cacaaaatac
     4141 ttcgttatat acacccccac agcttcattt ttaacagttt ctttaatctt atttattttt
     4201 ataaatccat tcgcccacaa ttccccaagc ttcttattat ctatatatgg gaccgagaac
     4261 agcaccaaat gataatggat tgctcccctc tgctggagtt cccaagttgc aatatattta
     4321 agctgttgat ttttcaagta ccttttcaat ctcttaataa ataaagtaaa ttcacgattt
     4381 gccctttcta tatcctgtat attttcttta aaagtaagag ttaaaaaggt acttttatca
     4441 tcataattac aatcaattat cctttttatt tcaaatcgct tgtcctggta atatgctttc
     4501 cttcgttgca tactttcagc ttt
//