LOCUS       Exported               11873 bp DNA     circular SYN 10-JUN-2022
DEFINITION  -.
ACCESSION   -
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 16, 2022 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             667..4802
                     /locus_tag="white dominant eye marker"
                     /label=white dominant eye marker
                     /note="white dominant eye marker"
     protein_bind    4878..4999
                     /locus_tag="5xUAS"
                     /label=5xUAS
     protein_bind    5000..5242
                     /locus_tag="Hsp70Bp"
                     /label=Hsp70Bp
     primer_bind     5024..5056
                     /locus_tag="hsp70.forward"
                     /label=hsp70.forward
     primer_bind     5239..5287
                     /locus_tag="dsarm(E).f"
                     /label=dsarm(E).f
     CDS             5263..8421
                     /locus_tag="dsarm isoform E"
                     /label=dsarm isoform E
     misc_feature    6589..6634
                     /locus_tag="primer mutagenesis"
                     /label=primer mutagenesis
     misc_feature    6610..6612
                     /locus_tag="mutation K193R K->R AAA->AGA"
                     /label=mutation K193R K->R AAA->AGA
     primer_bind     complement(8407..8451)
                     /locus_tag="dsarm(E).r"
                     /label=dsarm(E).r
     terminator      8633..9331
                     /locus_tag="SV40 terminator"
                     /label=SV40 terminator
     polyA_signal    9135..9326
                     /locus_tag="SV40 polyA"
                     /label=SV40 polyA
     misc_feature    9376..9660
                     /locus_tag="attB site"
                     /label=attB site
                     /note="attB site"
     gene            complement(10885..11745)
                     /locus_tag="AMP"
                     /label=AMP
                     /note="AMP"
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccaaaagc
     4861 tttccggatc caagcttgca tgcctgcagg tcggagtact gtcctccgag cggagtactg
     4921 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     4981 tccgagcgga gactctagcg agcgccggag tataaataga ggcgcttcgt ctacggagcg
     5041 acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac
     5101 aagcgcagct gaacaagcta aacaatctgc agtaaagtgc aagttaaagt gaatcaatta
     5161 aaagtaacca gcaaccaagt aaatcaactg caactactga aatctgccaa gaagtaatta
     5221 ttgaatacaa gaagagaact ctgaataggg aattgggaat tcatgtccaa tcaggcaccc
     5281 tggcccgttc gcaagggcat cttccgctcg agcggtcagt cggatttcac gccaacccga
     5341 tcgcctagtc ccattgtgga gatgccatta tccccgccgc cggtggccac gccctcgaat
     5401 agatttggaa tcaattcacc gctctcccca ccgccgcagc ctattcaagt ggtggcgggt
     5461 tcaacgacag ccactaccat gtccactgcg tccgctgcca gagtgtctgg tgcagctgcc
     5521 tccacttcat cctcgtcctc ctcttcttcg tcgtcgcagt gctcgtcgca aacctcgtcg
     5581 agttctagtt cgcacaccag agtacgtaaa agttctaacc cgcccccaca gccaattacc
     5641 agttgtccac tgtcgccacc accaccacca ccaccacagc aacaacaaca actaccacaa
     5701 caactgccac ctccaacgcc catcaacaac accaaccact cagcaattac aacacccaat
     5761 cacaacaata accacaactg caatcaaatg agaaatgtac gcgaaattcc cattgaggtg
     5821 gagcagagca aggaagccct ctcactccgc accggcgaca tcacacagca ggcgagcaac
     5881 aatgtggcgg cctccagtat tacggtgcaa agtgagaatt tctccgcgga caagaaggcg
     5941 atatcccagt cgcagcaatc gcagacgatg acctccaacg ggatcatcag tcaggagaaa
     6001 catgtatcct ccgcctcgca ggccaactac tcgatgtcgc acaagggcgt ttccagtacc
     6061 ggcagtagca tgatcaccag ctcctcgcaa atgtccgcga tgaatggcca gatgctgaag
     6121 ctcgccgatc tcaagttgga tgacctcaag tcgctgacag ctggcagtgg gcagcaggag
     6181 atcgagcaga ccatcaacaa gtattccaat atgctgacct caatagtgag ctccctgcaa
     6241 gaagatgagc gaggtggtag tgccataacc gtccacgatg ttggtggcaa gaagtctcag
     6301 tacctggaga agatcaacga agtcattcgt cgagcttggg cagtgcccac acatggccac
     6361 gagctgggct actcattgtg caattccctg cgccaaagtg gcggcctcga tcttctcatg
     6421 aagaactgcg ttaagcccga tctccagttt tcctccgcac agcttcttga gcagtgccta
     6481 accaccgaga accgcaaaca tgtggtggat aatggtctgg ataaggtggt caatgtggcc
     6541 tgtgtctgca caaagaattc caatatggag cactcccgcg tgggcactgg catcctggag
     6601 catctgttca gacactccga gggcacctgt tcggatgtga taaggctagg tggtctggat
     6661 gccgtgctct ttgagtgccg caccagtgat ttggaaactc tgcgacactg cgctagtgca
     6721 ctggcaaatt tatctctata tggtggagcc gagaatcagg aggagatgat cctacgcaag
     6781 gtgcccatgt ggttgttccc attggccttc cacaacgatg ataacatcaa gtactatgcc
     6841 tgcttggcca ttgcagtgct ggtagccaac aaggagattg aagccgaggt gctgaagtct
     6901 ggatgcttgg atttggtgga accctttgtc acctcccacg acccctcggc ttttgcgaga
     6961 tccaatctgg cacatgccca cgggcaaagc aaacattggc tgaaaagatt ggttccagtt
     7021 ttgagttcca atcgcgaaga ggcccgcaat ctggctgcct tccatttttg catggaggcg
     7081 ggcatcaaga gggagcaggg taacaccgac atcttccgcg agatcaacgc tattgaagct
     7141 ttgaaaaatg tggccagctg tccaaatgcc attgcatcca agttcgccgc tcaggctctg
     7201 agattgattg gagagacagt tccgcacaag ctgtcccaac aggttccctt gtggtccgtg
     7261 gaggatgtgc aggagtgggt aaagcaaatc ggtttcaatg actacatcga caagttcaac
     7321 gagtcccagg tggacgggga tcttttactg aagctcaatc aggataacct ccgggctgat
     7381 attggaatcg gtaatggaat acttctaaag cgttttgaac gcgagctgca aaatctcaag
     7441 cgtatggctg actactcatc caaggacacg gccaagatgc accaatttct ctcggagatc
     7501 ggtactgatt actgcaccta cacgtatgcc atgctgaatg ctggaattga caagtgtgca
     7561 ctgccgcatg tcaatgagga tatgctgatg acagagtgtg gcatccacaa ctcgatccat
     7621 cgtctgagaa ttctcaatgc ggtcaaaaac ttggagaatt cgctgcccag ctcgtcggag
     7681 gagaacatgg ccaagacgct agatgttttc gttagctata ggcgatcaaa cggctcccaa
     7741 ctagccagtc ttctcaaggt gcatctgcag cttcgtggtt tctccgtttt catcgacgtg
     7801 gagcgcctgg aggcgggcaa atttgacaac ggcctattga acagtattcg acaggcaaag
     7861 aactttgtct tagtattaac tcccgatgcc ctgcaccgct gcattaacga cgaggactgc
     7921 aaggactggg tgcatcgcga aatcgtggct gccctgaact cgaactgcaa cattataccc
     7981 atcatagacc agcaattcga ttggcccgag gtggagcgac ttcccgagga catgcgcagt
     8041 gtggcccatt ttaacggtgt taattggatc cacgactacc aggacgcatg catcgataag
     8101 ctcgaaagat ttttgcgcgg cgaaaagaat atcgatcgca ttgcggcgat ggtgcccggc
     8161 acccccggtt cggtctcata ccaaagaatg cacagcaacg actccgacta ccagagcgga
     8221 ggagcaggcg ccggatcagg agcaggaact ggaggaggag gtggtggcgg cgttacgggc
     8281 agtgtggtgg atggcctgat ggtggcggcc aatggcagcg gacaaggtgg gccaacgagt
     8341 actacatcta ctacttcttc tactcccaac tcaaattcaa gctcaagctc taaccagagt
     8401 cccccagcgg ctccagcgta ggaattccgg cgatgctgat ccgagcagtc cgatggggag
     8461 gaggaggagg aggaggagga cggcgatgag gaggaggaag atgatggatc cgatgaggaa
     8521 aatggaaatg gaaatagagg gggcagaaat cgacccagag acaacggagg atgagatgat
     8581 gatcccacat agacttgttg cattaaacga atattagcgc ttatgtctag aggatctttg
     8641 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     8701 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     8761 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
     8821 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
     8881 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
     8941 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     9001 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     9061 aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag
     9121 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     9181 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9241 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9301 ctcatcaatg tatcttatca tgtctggatc ggatccgcgg ccgcggctcg agggtacctc
     9361 tagagatcca ctagtgtcga cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca
     9421 gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt ccatcatgat
     9481 gaacgggtcg aggtggcggt agttgatccc ggcgaacgcg cggcgcaccg ggaagccctc
     9541 gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg ggacgtgcga cggcgtcggc
     9601 gggtgcggat acgcggggca gcgtcagcgg gttctcgacg gtcacggcgg gcatgtcgac
     9661 actagttcta gccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc
     9721 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     9781 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     9841 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     9901 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     9961 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10021 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10081 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10141 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10201 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10261 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10321 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10381 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10441 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10501 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10561 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10621 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10681 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10741 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    10801 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    10861 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10921 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10981 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    11041 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    11101 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11161 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11221 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11281 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11341 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    11401 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    11461 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    11521 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    11581 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    11641 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11701 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11761 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11821 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
