LOCUS       pUCM_AAVS1_flox(hygro) 11809 bp ds-DNA     circular     16-JUN-2022
DEFINITION  .
KEYWORDS    "marker:PuroR, AmpR"                                               
FEATURES             Location/Qualifiers
     CDS             54..122
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             123..914
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      1085..1673
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_recomb     complement(1721..2555)
                     /label="AAV 3' Arm"
                     /ApEinfo_revcolor=#800000
                     /ApEinfo_fwdcolor=#800000
     promoter        2585..2955
                     /label="TRE3G"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(2896..2915)
                     /label="101F TRE3G 1F"
                     /note="sequence: gtgttgtggaattgctccag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2967..2975
                     /label="Kozak"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             2976..4502
                     /label="NFIA CDS (nuclear factor 1 A-type isoform 1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             2976..4502
                     /label="Translation 2976-4502"
     primer          3364..3389
                     /label="NFIA_sequencingPrimer1 FWD"
                     /note="sequence: accttgttatggtgattttgtttaaa"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          4090..4109
                     /label="NFIA_sequencingPrimer2 FWD"
                     /note="sequence: catcacccattatccagcag"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             4503..4511
                     /label="Translation 4503-4511"
     misc_feature    4512..4565
                     /label="T2A"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             4512..4565
                     /label="Translation 4512-4565"
     primer          4554..4577
                     /label="121F"
                     /note="sequence: aatcccggccctatgaatctcctg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          4566..4583
                     /label="SOX9_sequencingPrimer1 FWD"
                     /note="sequence: atgaatctcctggacccc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             4566..6095
                     /label="SOX9 CDS (transcription factor SOX-9)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             4566..6095
                     /label="Translation 4566-6095"
     primer          4984..5001
                     /label="SOX9_sequencingPrimer2 FWD"
                     /note="sequence: gcaagctctggagacttc"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          5402..5418
                     /label="SOX9_sequencingPrimer3 FWD"
                     /note="sequence: cagcgacgtcatctcca"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          5819..5836
                     /label="SOX9_sequencingPrimer4 FWD"
                     /note="sequence: cgcctacagccccttcaa"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     polyA_signal    6118..6242
                     /label="SV40polyA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     polyA_signal    complement(6586..7040)
                     /label="RBGpolyA (minus last 72bp)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(7050..7796)
                     /label="rtTA3G"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     intron          7803..7896
                     /label="Mini-Intron"
                     /ApEinfo_revcolor=#a187be
                     /ApEinfo_fwdcolor=#a187be
     enhancer        complement(7904..8873)
                     /label="CAG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     enhancer        complement(8874..9151)
                     /label="CAG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     enhancer        9153..9532
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          9343..9365
                     /label="oSB073_GTSeq_pUCM-TO-hNGN2_FWD"
                     /note="sequence: caatggaaagtccctattggcgt"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     polyA_signal    complement(9538..9762)
                     /label="bGHpolyA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          9742..9762
                     /label="99R bGHpolyA_5'end REV"
                     /note="sequence: gctggcaactagaaggcacag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    9779..9818
                     /label="overhang"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    9779..11266
                     /label="geneblock"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    complement(9784..9817)
                     /label="loxP"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     CDS             complement(9827..9829)
                     /label="Translation 9829-9827"
     primer_bind     9830..9848
                     /label="iAA67 gia_1184_R"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(9830..10852)
                     /label="Hygro (NoCpG, codon-optimized)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     exon            complement(9830..10852)
                     /label="Translation 10852-9830"
     primer_bind     complement(9898..9917)
                     /label="F62"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     complement(10131..10148)
                     /label="iAA64 gia_843_F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     10319..10338
                     /label="iAA66 gia_674_R"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(10561..10580)
                     /label="F61"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     complement(10642..10661)
                     /label="iAA63 gia_332_F"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     10822..10843
                     /label="iAA65 gia_173_R"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             complement(10862..10915)
                     /label="T2A"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(10862..10915)
                     /label="Translation 10915-10862"
     misc_feature    10940..10966
                     /label="Splice Acceptor"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    complement(10971..11004)
                     /label="loxP"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_recomb     complement(11009..11809)
                     /label="AAV 5' Arm"
                     /ApEinfo_revcolor=#800000
                     /ApEinfo_fwdcolor=#800000
     primer          complement(11038..11059)
                     /label="oSB074_GTSeq_pUCM-FLOX_REV"
                     /note="sequence: atgtggctctggttctgggtac"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(11226..11248)
                     /label="oSB072_GTSeq_pUCM-TO-hNGN2_REV"
                     /note="sequence: ccccttacctctctagtctgtgc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    11227..11266
                     /label="overhang"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 aagggcgaat tcaaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
       61 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      121 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      181 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      241 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      301 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      361 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      421 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      481 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      541 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
      601 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
      661 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      721 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      781 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      841 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
      901 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      961 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1021 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1081 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1141 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1201 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1261 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1321 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1381 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1441 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1501 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1561 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1621 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1681 gcaacgcggc ctttttacgg ttcctggcga attcgccctt agagcagagc caggaacccc
     1741 tgtagggaag gggcaggaga gccaggggca tgagatggtg gacgaggaag ggggacaggg
     1801 aagcctgagc gcctctcctg ggcttgccaa ggactcaaac ccagaagccc agagcagggc
     1861 cttagggaag cgggaccctg ctctgggcgg aggaatatgt cccagatagc actggggact
     1921 ctttaaggaa agaaggatgg agaaagagaa agggagtaga ggcggccacg acctggtgaa
     1981 cacctaggac gcaccattct cacaaaggga gttttccaca cggacacccc cctcctcacc
     2041 acagccctgc caggacgggg ctggctactg gccttatctc acaggtaaaa ctgacgcacg
     2101 gaggaacaat ataaattggg gactagaaag gtgaagagcc aaagttagaa ctcaggacca
     2161 acttattctg attttgtttt tccaaactgc ttctcctctt gggaagtgta aggaagctgc
     2221 agcaccagga tcagtgaaac gcaccagaca gccgcgtcag agcagctcag gttctgggag
     2281 agggtagcgc agggtggcca ctgagaaccg ggcaggtcac gcatcccccc cttccctccc
     2341 accccctgcc aagctctccc tcccaggatc ctctctggct ccatcgtaag caaaccttag
     2401 aggttctggc aaggagagag atggctccag gaaatggggg tgtgtcacca gataaggaat
     2461 ctgcctaaca ggaggtgggg gttagaccca atatcaggag actaggaagg aggaggccta
     2521 aggatggggc ttttctgtca ccaatcctgt ccctagtaaa gctgatatct ccaccccaca
     2581 gtggtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     2641 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     2701 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     2761 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     2821 gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt
     2881 gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatacca actttccgta
     2941 ccacttccta ccctcgtaaa cttaagGCCG CCACCatgta ttctccgctc tgtctcaccc
     3001 aggatgaatt tcatcctttc atcgaagcac ttctgcccca cgtccgagcc tttgcctaca
     3061 catggttcaa cctgcaggcc cgaaaacgaa aatacttcaa aaaacatgaa aagcgtatgt
     3121 caaaagaaga agagagagcc gtgaaggatg aattgctaag tgaaaaacca gaggtcaagc
     3181 agaagtgggc atctcgactt ctggcaaagt tgcggaaaga tatccgaccc gaatatcgag
     3241 aggattttgt tcttacagtt acagggaaaa aacctccatg ttgtgttctt tccaacccag
     3301 accagaaagg caagatgcga agaattgact gcctccgcca ggcagataaa gtctggaggt
     3361 tggaccttgt tatggtgatt ttgtttaaag gtattccgct ggaaagtact gatggcgagc
     3421 gccttgtaaa gtccccacaa tgctctaatc cagggctctg tgtccaaccc catcacatag
     3481 gggtttctgt taaggaactc gatttatatt tggcatactt tgtgcatgca gcagattcaa
     3541 gtcaatctga aagtcccagc cagccaagtg acgctgacat taaggaccag ccagaaaatg
     3601 gacatttggg cttccaggac agttttgtca catcaggtgt ttttagtgtc actgagctag
     3661 taagagtgtc acagacacca atagctgcag gaactggccc aaatttttct ctctcagatt
     3721 tggaaagttc ttcatactac agcatgagtc caggagcaat gaggaggtct ttacccagca
     3781 catcctctac gagctccaca aagcgcctca agtctgtgga ggatgaaatg gacagtcctg
     3841 gtgaggagcc attttataca ggccaagggc gctccccagg aagtggcagt cagtcaagtg
     3901 gatggcatga agtggagcca ggaatgccat ctccaaccac actgaagaag tcggagaagt
     3961 ctggtttcag cagcccctcc ccttcacaga cctcctccct gggaacggcg ttcacacagc
     4021 atcaccgacc tgtcattaca ggacccagag caagtccgca tgcaacacca tcgactcttc
     4081 atttcccgac atcacccatt atccagcagc ctgggcctta cttctcacac ccagccatcc
     4141 gctatcaccc tcaggagacg ctgaaagaat ttgtccaact tgtctgccct gatgctggtc
     4201 agcaggctgg acaggtgggg ttcctcaatc ccaatgggag cagccaaggc aaggtgcaca
     4261 acccattcct tcccacccca atgttgccac cgccaccgcc accaccgatg gccaggcctg
     4321 tgcctctgcc ggtgccagac acaaagcctc caaccacgtc aacagaagga ggtgcagcct
     4381 cccccacgtc accaacctac tcgacaccca gcacctcccc cgcaaaccga ttcgtcagtg
     4441 ttggaccacg ggatccaagc tttgtaaata tccctcaaca gacacagtcc tggtacctgg
     4501 gaggtagcgg agagggcaga ggcagtctgc tgacatgcgg tgacgtggaa gagaatcccg
     4561 gccctatgaa tctcctggac cccttcatga agatgaccga cgagcaggag aagggcctgt
     4621 ccggcgcccc cagccccacc atgtccgagg actccgcggg ctcgccctgc ccgtcgggct
     4681 ccggctcgga caccgagaac acgcggcccc aggagaacac gttccccaag ggcgagcccg
     4741 atctgaagaa ggagagcgag gaggacaagt tccccgtgtg catccgcgag gcggtcagcc
     4801 aggtgctcaa aggctacgac tggacgctgg tgcccatgcc ggtgcgcgtc aacggctcca
     4861 gcaagaacaa gccgcacgtc aagcggccca tgaacgcctt catggtgtgg gcgcaggcgg
     4921 cgcgcaggaa gctcgcggac cagtacccgc acttgcacaa cgccgagctc agcaagacgc
     4981 tgggcaagct ctggagactt ctgaacgaga gcgagaagcg gcccttcgtg gaggaggcgg
     5041 agcggctgcg cgtgcagcac aagaaggacc acccggatta caagtaccag ccgcggcgga
     5101 ggaagtcggt gaagaacggg caggcggagg cagaggaggc cacggagcag acgcacatct
     5161 cccccaacgc catcttcaag gcgctgcagg ccgactcgcc acactcctcc tccggcatga
     5221 gcgaggtgca ctcccccggc gagcactcgg ggcaatccca gggcccaccg accccaccca
     5281 ccacccccaa aaccgacgtg cagccgggca aggctgacct gaagcgagag gggcgcccct
     5341 tgccagaggg gggcagacag ccccctatcg acttccgcga cgtggacatc ggcgagctga
     5401 gcagcgacgt catctccaac atcgagacct tcgatgtcaa cgagtttgac cagtacctgc
     5461 cgcccaacgg ccacccgggg gtgccggcca cgcacggcca ggtcacctac acgggcagct
     5521 acggcatcag cagcaccgcg gccaccccgg cgagcgcggg ccacgtgtgg atgtccaagc
     5581 agcaggcgcc gccgccaccc ccgcagcagc ccccacaggc cccgccggcc ccgcaggcgc
     5641 ccccgcagcc gcaggcggcg cccccacagc agccggcggc acccccgcag cagccacagg
     5701 cgcacacgct gaccacgctg agcagcgagc cgggccagtc ccagcgaacg cacatcaaga
     5761 cggagcagct gagccccagc cactacagcg agcagcagca gcactcgccc caacagatcg
     5821 cctacagccc cttcaacctc ccacactaca gcccctccta cccgcccatc acccgctcac
     5881 agtacgacta caccgaccac cagaactcca gctcctacta cagccacgcg gcaggccagg
     5941 gcaccggcct ctactccacc ttcacctaca tgaaccccgc tcagcgcccc atgtacaccc
     6001 ccatcgccga cacctctggg gtcccttcca tcccgcagac ccacagcccc cagcactggg
     6061 aacaacccgt ctacacacag ctcactcgac cttgaatcga tcgcccaccc cAACTTGttt
     6121 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6181 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6241 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6301 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     6361 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     6421 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     6481 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6541 gttcggctgc ggcgagcggt atcagctcac tcaaaggacg gccgcacatg taaggaaaat
     6601 tttagggatg ttaaagaaaa aaataacaca aaacaaaata taaaaaaaat ctaacctcaa
     6661 gtcaaggctt ttctatggaa taaggaatgg acagcagggg gctgtttcat atactgatga
     6721 cctctttata gccaaccttt gttcatggca gccagcatat gggcatatgt tgccaaactc
     6781 taaaccaaat actcattctg atgttttaaa tgatttgccc tcccatatgt ccttccgagt
     6841 gagagacaca aaaaattcca acacactatt gcaatgaaaa taaatttcct ttattagcca
     6901 gaagtcagat gctcaagggg cttcatgatg tccccataat ttttggcaga gggaaaaaga
     6961 tctcagtggt atttgtgagc cagggcattg gccacaccag ccaccacctt ctgataggca
     7021 gcctgcacct gaggagtgaa ttcacgcgtt tagcctggca gcatatcgag atcgaagtca
     7081 tcgagagcgt cagcagggag catatccaga tcaaaatcat ccagggcatc ggcgggcagc
     7141 atgtccaggt cgaagtcgtc cagggcgtcg gtgggtccgc cgctctcgca cttcagctgc
     7201 ttctcgaggc cgcagatgat cagttccagg ccgaacagga aggcgggctc ggcgccctgt
     7261 ctgtcgaaca gctcgatggc ctgcttcagc agggggggca tgctgtcggt ggtgggtgtc
     7321 tctctttcct ctttggcgac ctggtgttcc tgttcttcca gcacgcagcc cagggtgaag
     7381 tggcccacgg cgctcagggc gtacagggcg ttttccaggc tgaagccctg ctggcacagg
     7441 aaggccagct ggttttccag tgtctcgtac tgcttctcgg tgggtctggt gcccaggtgc
     7501 actttggcgc cgtcccggtg gctcagcagg gcgcatctgt agctcttggc gttgttccgc
     7561 aggaagtcct gccagctctc gccttccaga gggcagctgt gggtgtggtg ccggtccagc
     7621 atctcgatgg gcagggcgtc cagcagggcc cgcttgttct tcacgtgcca gtacagggtg
     7681 ggctgttcca cgcccagttt ctgggccagc ttccgggtgg tcaggccctc gatgcccacg
     7741 ccgttcagca gttccagggc gctgttgatc actttgctct tgtccagccg gctcatgtcg
     7801 acattctttg ccaaaatgat gagacagcac aataaccagc acgttgccca ggagctgtag
     7861 gaaaaagaag aaggcatgaa catggttagc agaggctcta gagccgccgg tcacacgcca
     7921 gaagccgaac cccgccctgc cccgtccccc ccgaaggcag ccgtcccccc gcggacagcc
     7981 ccgaggctgg agagggagaa ggggacggcg gcgcggcgac gcacgaaggc cctccccgcc
     8041 catttccttc ctgccggcgc cgcaccgctt cgccccgcgc ccgctagagg gggtgcggcg
     8101 gcgcctccca gatttcggct ccgcacagat ttgggacaaa ggaagtccct gcgccctctc
     8161 gcacgattac cataaaaggc aatggctgcg gctcgccgcg cctcgacagc cgccggcgct
     8221 ccgggggccg ccgcgcccct cccccgagcc ctccccggcc cgaggcggcc ccgccccgcc
     8281 cggcaccccc acctgccgcc accccccgcc cggcacggcg agccccgcgc cacgccccgt
     8341 acggagcccc gcacccgaag ccgggccgtg ctcagcaact cggggagggg ggtgcagggg
     8401 gggttgcagc ccgaccgacg cgcccacacc ccctgctcac ccccccacgc acacaccccg
     8461 cacgcagcct ttgttcccct cgcagccccc cccgcaccgc ggggcaccgc ccccggccgc
     8521 gctcccctcg cgcacactgc ggagcgcaca aagccccgcg ccgcgcccgc agcgctcaca
     8581 gccgccgggc agcgcggagc cgcacgcggc gctccccacg cacacacaca cgcacgcacc
     8641 ccccgagccg ctccccccgc acaaagggcc ctcccggagc ccctcaaggc tttcacgcag
     8701 ccacagaaaa gaaacaagcc gtcattaaac caagcgctaa ttacagcccg gaggagaagg
     8761 gccgtcccgc ccgctcacct gtgggagtaa cgcggtcagt cagagccggg gcgggcggcg
     8821 cgaggcggcg gcggagcggg gcacggggcg aaggcagcgt cgcagcgact ccccgcccgc
     8881 cgcgcgcttc gctttttata gggccgccgc cgccgccgcc tcgccataaa aggaaacttt
     8941 cggagcgcgc cgctctgatt ggctgccgcc gcacctctcc gcctcgcccc gccccgcccc
     9001 tcgccccgcc ccgccccgcc tggcgcgcgc cccccccccc cccccgcccc catcgctgca
     9061 caaaataatt aaaaaataaa taaatacaaa attgggggtg gggagggggg ggagatgggg
     9121 agagtgaagc agaacgtggg gctcacctcg accatggtaa tagcgatgac taatacgtag
     9181 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     9241 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     9301 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     9361 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     9421 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     9481 actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg tcgagaccca
     9541 tagagcccac cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt
     9601 cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt
     9661 cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg
     9721 gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcggg
     9781 tttataactt cgtataatgt atgctatacg aagttatGGT ACCTTATTAt tccttggctc
     9841 tgggtcttgt ggagggtctc ctgtttccag agtcagccag aacttcaaca catccatcag
     9901 tccaaacagc agcagacctt cttgcaattt gagttcttcc aacagttcca gcaccagacc
     9961 tcacaatggc atcacatctt ccttgtgccc aagcagcatc atcaaagttt ccatcaacca
    10021 gagattgata cagttggtcc aggccaattc tgagcatgta ggctctcagt ctgggggaac
    10081 cagccagttc tgggtgtctt ctttcaaaat atcttgtttg ttgttccatg caagccagcc
    10141 aaggtctcca aaaaaaaatg ttggcaacct catattgaga atctccaaac atggcttcag
    10201 accagtcaat gactgcagtg attctgccat tgtctgtcag aacattgttg cttccaaaat
    10261 cagcatggac caggtgtctg acttcaggac aatcttctgc ccacagcatg agttcatcca
    10321 gtgcctgagc aacagaagca gaaactgtgt catccatcac agtctgccag tgatagacat
    10381 gaggatcagc aatggcacaa atgaaatccc tccaagtggt gtactgaccg atgccttggg
    10441 gaccaaaagg accaaatccg ctggtttggc tcagatcagc tgctgcaatg gcatccattg
    10501 cttcagcaac aggttgcaga acagctggca gctcagtttc agggagatcc tggagagtga
    10561 ctccttgtgc tcttctgctg atgcagtagg tgagagattc agaaaattct ccaatgtcca
    10621 gaacttctgg aattggcaga gcagcagagg caaagtgtct gtaaacatat ctgtctttgt
    10681 aaaaaccatc agcacaagaa ttgaccctca gaacataacc tcttcctcca acatcaaaag
    10741 aaaaggctct gctttcttca ccttcagaca gctgcatgag atcagaaaca gaatcaaatt
    10801 tttcaatgag aaacttctca acagaagttg ctgtcagttc aggtttcttc atctcgagcc
    10861 tagggccggg attctcctcc acgtcaccgc atgttagaag acttcctctg ccctctccgc
    10921 tgccagatct ctcgaggccc tgtgggagga agagaagagg tcagaagctt ataacttcgt
    10981 ataatgtatg ctatacgaag ttattgcccc actgtggggt ggaggggaca gataaaagta
    11041 cccagaacca gagccacatt aaccggccct gggaatataa ggtggtccca gctcggggac
    11101 acaggatccc tggaggcagc aaacatgctg tcctgaagtg gacatagggg cccgggttgg
    11161 aggaagaaga ctagctgagc tctcggaccc ctggaagatg ccatgacagg gggctggaag
    11221 agctagcaca gactagagag gtaagggggg taggggagct gcccaaatga aaggagtgag
    11281 aggtgacccg aatccacagg agaacggggt gtccaggcaa agaaagcaag aggatggaga
    11341 ggtggctaaa gccagggaga cggggtactt tggggttgtc cagaaaaacg gtgatgatgc
    11401 aggcctacaa gaaggggagg cgggacgcaa gggagacatc cgtcggagaa ggccatccta
    11461 agaaacgaga gatggcacag gccccagaag gagaaggaaa agggaaccca gcgagtgaag
    11521 acggcatggg gttgggtgag ggaggagaga tgcccggaga ggacccagac acggggagga
    11581 tccgctcaga ggacatcacg tggtgcagcg ccgagaagga agtgctccgg aaagagcatc
    11641 cttgggcagc aacacagcag agagcaaggg gaagagggag tggaggaaga cggaacctga
    11701 aggaggcggc agggaaggat ctgggccagc cgtagaggtg acccaggcca caagctgcag
    11761 acagaaagcg gcacaggccc aggggagaga atgctggtca gagaaagca
//