LOCUS       Exported                6839 bp ds-DNA     circular SYN 24-OCT-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 16, 2022 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             613..1323
                     /codon_start=1
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     primer_bind     1015..1033
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1324..1338
                     /codon_start=1
                     /label=linker
                     /translation="SGLRS"
     CDS             1339..3453
                     /codon_start=1
                     /label=Synapsin 1a (rat)
                     /translation="MNYLRRRLSDSNFMANLPNGYMTDLQRPQPPPPPPSAASPGATPG
                     SAAASAERASTAAPVASPAAPSPGSSGGGGFFSSLSNAVKQTTAAAAATFSEQVGGGSG
                     GAGRGGAAARVLLVIDEPHTDWAKYFKGKKIHGEIDIKVEQAEFSDLNLVAHANGGFSV
                     DMEVLRNGVKVVRSLKPDFVLIRQHAFSMARNGDYRSLVIGLQYAGIPSVNSLHSVYNF
                     CDKPWVFAQMVRLHKKLGTEEFPLIDQTFYPNHKEMLSSTTYPVVVKMGHAHSGMGKVK
                     VDNQHDFQDIASVVALTKTYATAEPFIDAKYDVRVQKIGQNYKAYMRTSVSGNWKTNTG
                     SAMLEQIAMSDRYKLWVDTCSEIFGGLDICAVEALHGKDGRDHIIEVVGSSMPLIGDHQ
                     DEDKQLIVELVVNKMTQALPRQRDASPGRGSHSQTPSPGALPLGRQTSQQPAGPPAQQR
                     PPPQGGPPQPGPGPQRQGPPLQQRPPPQGQQHLSGLGPPAGSPLPQRLPSPTAAPQQSA
                     SQATPMTQGQGRQSRPVAGGPGAPPAARPPASPSPQRQAGPPQATRQASISGPAPPKVS
                     GASPGGQQRQGPPQKPPGPAGPIRQASQAGPGPRTGPPTTQQPRPSGPGPAGRPTKPQL
                     AQKPSQDVPPPIIAAAGGPPHPQLNKSQSLTNAFNLPEPAPPRPSLSQDEVKAETIRSL
                     RKSFASLFSD"
     polyA_signal    3627..3748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3755..4210)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4237..4341
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4343..4700
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4551..4686
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4735..5529
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5761..5808
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6137..6725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgtc taagggcgaa gagctgatca aggaaaatat gcgtatgaag
      661 gtggtcatgg aaggttcggt caacggccac caattcaaat gcacaggtga aggagaaggc
      721 agaccgtacg agggagtgca aaccatgagg atcaaagtca tcgagggagg acccctgcca
      781 tttgcctttg acattcttgc cacgtcgttc atgtatggca gccgtacctt tatcaagtac
      841 ccggccgaca tccctgattt ctttaaacag tcctttcctg agggttttac ttgggaaaga
      901 gttacgagat acgaagatgg tggagtcgtc accgtcacgc aggacaccag ccttgaggat
      961 ggcgagctcg tctacaacgt caaggtcaga ggggtaaact ttccctccaa tggtcccgtg
     1021 atgcagaaga agaccaaggg ttgggagcct aatacagaga tgatgtatcc agcagatggt
     1081 ggtctgagag gatacactga catcgcactg aaagttgatg gtggtggcca tctgcactgc
     1141 aacttcgtga caacttacag gtcaaaaaag accgtcggga acatcaagat gcccggtgtc
     1201 catgccgttg atcaccgcct ggaaaggatc gaggagagtg acaatgaaac ctacgtagtg
     1261 caaagagaag tggcagttgc caaatacagc aaccttggtg gtggcatgga cgagctgtac
     1321 aagtccggac tcagatctat gaactacctg cggcgccgcc tgtcggacag caacttcatg
     1381 gccaatctgc ctaatgggta tatgacagac ctgcagcgcc cgcaaccacc tccgccgccg
     1441 ccctcagccg ccagccctgg ggccactccc ggatccgctg ctgcctctgc cgagagggcc
     1501 tccacagctg ctccagtggc ctctccagca gcccctagtc ccgggtcctc ggggggcggt
     1561 ggcttcttct cctcgctgtc taacgcggtc aaacagacca cagcagctgc agccgccacc
     1621 ttcagtgagc aggtgggcgg tggctctggg ggcgcaggcc gcgggggcgc cgccgccagg
     1681 gtgctgctgg tcatcgacga gccgcacacc gactgggcaa aatacttcaa agggaagaag
     1741 atccatggag aaattgacat taaagtagag caagctgaat tctccgatct caatcttgtg
     1801 gctcatgcca atggtggatt ctccgtggac atggaagttc ttcggaatgg ggtcaaagtt
     1861 gtgaggtctc tgaagccaga ctttgtgctg atccgccagc atgccttcag catggcacgt
     1921 aatggagact accgcagttt ggtcattggg ctgcagtatg ctgggatccc cagtgttaac
     1981 tctttgcatt ctgtctacaa cttttgtgac aaaccctggg tgtttgccca gatggttcga
     2041 ctacacaaga agcttggaac agaggaattc cctctgattg atcagacttt ctatcccaat
     2101 cataaagaga tgctcagcag cacaacatac cctgtagttg tgaagatggg ccacgcacac
     2161 tctgggatgg gcaaggtcaa ggtagacaac caacatgact tccaggatat tgcaagtgtt
     2221 gtggcactga ctaagacata tgccactgct gagcccttca ttgatgctaa atacgatgtg
     2281 cgtgtccaga agattgggca gaactacaag gcctacatga ggacatcagt gtcagggaac
     2341 tggaagacca atacaggctc tgctatgctt gagcagattg ctatgtctga caggtacaag
     2401 ttgtgggtag acacgtgctc agagattttt gggggacttg acatctgcgc agtggaagca
     2461 ctgcatggca aggacggaag ggatcacatt attgaggtgg tgggctcctc catgccactc
     2521 attggggatc accaggatga agacaagcag ctcatcgtgg aacttgtggt caacaagatg
     2581 actcaggctc tgcctcggca gcgggatgct tcccctggca ggggttccca cagccagact
     2641 ccatccccag gagccctgcc cttgggccgc cagacctccc agcagcctgc aggacctcct
     2701 gctcaacaac gacccccacc ccagggaggc cctccacaac caggcccagg acctcagcgc
     2761 cagggacccc cgctgcaaca gcgcccaccc ccacaaggcc agcaacatct ttctggcctt
     2821 ggacccccag ctggcagccc tctgccccag cgcctaccaa gtcccacagc agcacctcag
     2881 cagtccgcct ctcaggccac accaatgacc cagggtcaag gccgccagtc acggccagtg
     2941 gcaggaggcc ccggagcacc tccagcagcc cgcccgccgg cctccccatc tccacagcgt
     3001 caggcagggc ccccacaggc tacccgtcag gcatctatct ctggtccagc tccaccgaag
     3061 gtctcaggag cctcacccgg agggcagcag cgccaaggcc ctccccagaa acccccaggc
     3121 cctgctggtc ccattcgtca ggccagccag gcaggtcccg gacctcgcac tgggccaccc
     3181 accacacagc agccccggcc cagcggccca ggtcctgctg gacgtcccac caaaccacag
     3241 ctggctcaga aacccagcca ggatgtgcca ccacccatca ttgctgctgc cgggggaccc
     3301 ccgcaccccc agctcaacaa atcccagtct ctgaccaatg ccttcaacct tccagagcca
     3361 gccccgccca ggcccagcct tagccaggat gaggtgaaag ctgagaccat ccgcagcctg
     3421 aggaagtctt tcgccagcct cttctccgac tgagagctca agcttcgaat tctgcagtcg
     3481 acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc agccatacca
     3541 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     3601 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     3661 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3721 gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta
     3781 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3841 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3901 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3961 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     4021 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     4081 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     4141 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     4201 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4261 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4321 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     4381 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4441 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4501 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4561 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4621 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4681 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     4741 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4801 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4861 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     4921 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     4981 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     5041 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     5101 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     5161 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     5221 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     5281 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     5341 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5401 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5461 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5521 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     5581 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5641 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5701 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     5761 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     5821 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     5881 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     5941 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     6001 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6061 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6121 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6181 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6241 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     6301 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6361 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6421 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6481 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6541 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6601 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6661 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6721 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6781 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
