LOCUS       Exported                4637 bp DNA     circular SYN 18-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 18, 2022 from SnapGene Viewer 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    202..413
                     /label=Polymerase I promoter
     misc_feature    414..516
                     /label=5' UTR
     CDS             complement(517..2169)
                     /codon_start=1
                     /label=luciferase ORF
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    2170..2222
                     /label=3' UTR
     misc_feature    2223..2254
                     /label=Polymerase I terminator
     misc_feature    2606..2692
                     /label=rrnB T1 terminator
     misc_feature    2784..2811
                     /label=rrnB T2 terminator
     misc_feature    2830..2921
                     /label=AmpR promoter
     CDS             2922..3782
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    3953..4542
                     /label=ori
ORIGIN
        1 gatcacgatc ctttctggcg agtccccgtg cggagtcgga gagcgctccc tgagcgcgcg
       61 tgcggcccga gaggtcgcgc ctggccggcc ttcggtccct cgtgtgtccc ggtcgtagga
      121 ggggccggcc gaaaatgctt ccggctcccg ctctggagac acgggccggc cccctgcgtg
      181 tggcacgggc ggccgggagg gcgtccccgg cccggcgctg ctcccgcgtg tgtcctgggg
      241 ttgaccagag ggccccgggc gctccgtgtg tggctgcgat ggtggcgttt ttggggacag
      301 gtgtccgtgt cgcgcgtcgc ctgggccggc ggcgtggtcg gtgacgcgac ctcccggccc
      361 cgggggaggt atatctttcg ctccgagtcg gcattttggg ccgccgggtt attagtagta
      421 acaagagcat ttttcagaaa caattaagtt tagtaaggac aattgttcaa acaaaacagg
      481 aacaaagggt ttagaacaga ctcaaccatt cctccattac acggcgatct ttccgccctt
      541 cttggccttt atgaggatct ctctgatttt tcttgcgtcg agttttccgg taagaccttt
      601 cggtacttcg tccacaaaca caactcctcc gcgcaacttt ttcgcggttg ttacttgact
      661 ggcgacgtaa tccacgatct ctttttccgt catcgtcttt ccgtgctcca aaacaacaac
      721 ggcggcggga agttcaccgg cgtcatcgtc gggaagacct gcgacacctg cgtcgaagat
      781 gttggggtgt tggagcaaga tggattccaa ttcagcggga gccacctgat agcctttgta
      841 cttaatcaga gacttcaggc ggtcaacgat gaagaagtgt tcgtcttcgt cccagtaagc
      901 tatgtctcca gaatgtagcc atccatcctt gtcaatcaag gcgttggtcg cttccggatt
      961 gtttacataa ccggacataa tcataggacc tctcacacac agttcgcctc tttgattaac
     1021 gcccagcgtt ttcccggtat ccagatccac aaccttcgct tcaaaaaatg gaacaacttt
     1081 accgaccgcg cccggtttat catccccctc gggtgtaatc agaatagctg atgtagtctc
     1141 agtgagccca tatccttgcc tgatacctgg cagatggaac ctcttggcaa ccgcttcccc
     1201 gacttcctta gagaggggag cgccaccaga agcaatttcg tgtaaattag ataaatcgta
     1261 tttgtcaatc agagtgcttt tggcgaagaa ggagaatagg gttggcacca gcagcgcact
     1321 ttgaatcttg taatcctgaa ggctcctcag aaacagctct tcttcaaatc tatacattaa
     1381 gacgactcga aatccacata tcaaatatcc gagtgtagta aacattccaa aaccgtgatg
     1441 gaatggaaca acacttaaaa tcgcagtatc cggaatgatt tgattgccaa aaataggatc
     1501 tctggcatgc gagaatctca cgcaggcagt tctatgaggc agagcgacac ctttaggcag
     1561 accagtagat ccagaggagt tcatgatcag tgcaattgtc ttgtccctat cgaaggactc
     1621 tggcacaaaa tcgtattcat taaaaccggg aggtagatga gatgtgacga acgtgtacat
     1681 cgactgaaat ccctggtaat ccgttttaga atccatgata ataatttttt ggatgattgg
     1741 gagctttttt tgcacgttca aaattttttg caaccccttt ttggaaacga acaccacggt
     1801 aggctgcgaa atgcccatac tgttgagcaa ttcacgttca ttataaatgt cgttcgcggg
     1861 cgcaactgca actccgataa ataacgcgcc caacaccggc ataaagaatt gaagagagtt
     1921 ttcactgcat acgacgattc tgtgatttgt attcagccca tatcgtttca tagcttctgc
     1981 caaccgaacg gacatttcga agtactcagc gtaagtgatg tccacctcga tatgtgcatc
     2041 tgtaaaagca attgttccag gaaccagggc gtatctcttc atagccttat gcagttgctc
     2101 tccagcggtt ccatcttcca gcggatagaa tggcgccggg cctttcttta tgtttttggc
     2161 gtcttccatt gttcattttt ggcctatttg cttcagtttt gagaagatgc tctgcttctg
     2221 ctccccccca acttcggagg tcgaccagta ctccgggcga cactttgttt ttttttttcc
     2281 cccgatgctg gaggtcgacc agatgtccga aagtgtcccc cccccccccc cccccggcgc
     2341 ggagcggcgg ggccactctg gactcttttt tttttttttt ttttggggat cggccgctag
     2401 cttggctgtt ttggcggatg aaagaagatt ttcagcctga tacagattaa atcagaacgc
     2461 agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac
     2521 cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat
     2581 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2641 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
     2701 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
     2761 aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct
     2821 acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2881 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2941 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3001 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3061 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttcccatga
     3121 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     3181 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     3241 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     3301 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     3361 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     3421 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     3481 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     3541 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     3601 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     3661 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     3721 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3781 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     3841 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3901 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3961 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa ancaccgcta ccagcggngg
     4021 tttgnttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagac
     4081 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     4141 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4201 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4261 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4321 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4381 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4441 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4501 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4561 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4621 cctgattcat tactcga
//
