LOCUS       Exported                7980 bp DNA     circular SYN 26-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 26, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
COMMENT     LOCUS       MIGRII                  6462 bp    DNA     circular     
            14-NOV-2006

            SOURCE      

              ORGANISM  

            COMMENT     This file is created by Vector NTI

                        http://www.invitrogen.com/

            COMMENT     VNTDATE|425052980|

            COMMENT     VNTDBDATE|427051417|

            COMMENT     LSOWNER|

            COMMENT     VNTNAME|MIGRII|

            COMMENT     VNTAUTHORNAME|auto|

            COMMENT     VNTREPLTYPE|Plasmid

            COMMENT     VNTREPLTYPE|Virus

            COMMENT     VNTEXTCHREPL|Bacteria

            COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..515
                     /label=5'LTR
     misc_feature    516..1404
                     /label=packaging signal
     primer_bind     1333..1353
                     /label=MSCV-5'
     misc_feature    1455..1720
                     /label=hU6 promoter
                     /note="hU6 promoter"
     misc_feature    2075..2380
                     /label=ccdB
                     /note="ccdB"
     misc_feature    2433..2525
                     /label=iCRISPR scaffold
                     /note="iCRISPR scaffold"
     misc_feature    2532..3079
                     /label=mPGK promoter
                     /note="mPGK promoter"
     misc_feature    3080..3748
                     /label=Puro-2A-BFP
                     /note="Puro-2A-BFP"
     misc_feature    3695..3748
                     /label=T2A
                     /note="T2A"
     misc_feature    3749..4276
                     /label=Thy1.1
                     /note="Thy1.1"
     LTR             4372..4855
                     /label=3' LTR
     rep_origin      5368..6041
                     /direction=RIGHT
                     /label=ColE1
     misc_feature    complement(6189..7046)
                     /label=AmpR
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggatccagtt tggttagtac
     1441 cgggccctac gcgtccaagg tcgggcagga agagggccta tttcccatga ttccttcata
     1501 tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac
     1561 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     1621 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
     1681 tttcttggct ttatatatct tgtggaaagg acgaaacacc gggtcttccg taatctagag
     1741 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     1801 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1861 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1921 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1981 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     2041 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     2101 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     2161 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga
     2221 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     2281 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     2341 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     2401 cagccagtct gcaggtcgac catagaagac acgtttaaga gctatgctgg aaacagcata
     2461 gcaagtttaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     2521 tttttggatc caattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     2581 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     2641 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     2701 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     2761 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     2821 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     2881 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     2941 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     3001 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcatcc atctagatct
     3061 cgagcagctg aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3121 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     3181 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     3241 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     3301 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     3361 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     3421 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     3481 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     3541 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     3601 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     3661 ccgcaagccc ggtgccggcg gcgggtccgg aggagagggc agaggaagtc tcctaacatg
     3721 cggtgacgtg gaggagaatc ctggcccaac catgggtctt ttctgcagtc accgtcctcg
     3781 aggcaccatg aacccagcca tcagcgtcgc tctcctgctc tcagtcttgc aggtgtcccg
     3841 agggcagaag gtgaccagcc tgacagcctg cctggtgaac caaaaccttc gcctggactg
     3901 ccgccatgag aataacacca aggataactc catccagcat gagttcagcc tgacccgaga
     3961 gaagaggaag cacgtgctct caggcaccct tgggataccc gagcacacgt accgctcccg
     4021 cgtcaccctc tccaaccagc cctatatcaa ggtccttacc ctagccaact tcaccaccaa
     4081 ggatgagggc gactactttt gtgagcttcg cgtctcgggc gcgaatccca tgagctccaa
     4141 taaaagtatc agtgtgtata gagacaagct ggtcaagtgt ggcggcataa gcctgctggt
     4201 tcagaacaca tcctggatgc tgctgctgct gctttccctc tccctcctcc aagccctgga
     4261 cttcatttct ctgtgagtcg acctgcagcc aagctatcga taaaataaaa gattttattt
     4321 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa
     4381 gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa
     4441 ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc
     4501 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt
     4561 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     4621 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     4681 tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc
     4741 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     4801 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt
     4861 aacagtttct tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc
     4921 ctgactcaat tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt
     4981 atggacagcg cagaagagct ggggagaatt aattcgtaat catggtcata gctgtttcct
     5041 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5101 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5161 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5221 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5281 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5341 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5401 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5461 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5521 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5581 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5641 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5701 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5761 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5821 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     5881 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5941 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6001 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6061 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6121 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6181 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6241 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6301 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6361 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6421 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6481 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6541 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6601 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6661 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6721 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6781 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6841 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6901 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6961 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7021 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7081 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7141 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     7201 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     7261 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     7321 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     7381 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     7441 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     7501 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     7561 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     7621 cgttgtaaaa cgacggcgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     7681 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     7741 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     7801 cgccggtgat gccggccacg atgcgtccgg cgtagaggcg attagtccaa tttgttaaag
     7861 acaggatatc agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta
     7921 tagagtacga gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa
//
