LOCUS       pSLPB2B-NLS-ecDHFR-linker-dCas9-tagRFPt-P2A-tagBFP-pGK-Blasticidin_Bv2       11929 bp    DNA     circular UNA 01-FEB-2022
DEFINITION  SP149: B v2.
ACCESSION   SP149
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        complement(650..754)
                     /gene="AmpRp; bla"
                     /note="AmpR promoter; PROMOTER AmpR promoter; color:
                     #ffffff; direction: LEFT"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /X-Transferred_From="2014); Digestion of  pU6-sgGAL4-1
                     EF1Alpha-puro-T2A-BFP 3XUAS-mTK-mCherry (adopted Gilbert
                     2014) - Fragment 2; Digestion of  pU6-sgGAL4-1
                     EF1Alpha-puro-T2A-BFP 3XUAS-mTK-mCherry (adopted Gilbert
                     2014) - Fragment 3; Fire_reporter; Tet-inducible
                     Luciferase reporter; Tet-pLKO-puro Sequences;
                     lentiGuide-Puro; lentiGuide-Puro Dusp1 guide 1;
                     lentiGuide-Puro Fgfr2 guide 1; lentiGuide-Puro Spry4 guide
                     1; pEGIP; pHR-SFFV-KRAB-dCas9-P2A-mCherry;
                     pHR-SFFV-KRAB-dCas9-P2A-mCherry (Gilbert et al.; pLenti
                     PGK GFP Blast; pLenti PGK GFP Puro; pU6-sgGAL4-1
                     EF1Alpha-puro-T2A-BFP 3XUAS-mTK-mCherry (adopted Gilbert
                     2014)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="lentiGuide-Puro, pSLQ2812_pPB:
                     CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9 
                     (modified), pSLQ2817_pPB:
                     CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
                     (modified), pSLQ2818_pPB:
                     CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
                     , pSLQ2833_CAG-PYL1-VPR-IRES-Zeocin-WPRE-SV40PA
                     PGK-ABI-tagBFP-SadCas9 (modified), pTRETightBI-RY-0,
                     pU6-sgGAL4-1 EF1Alpha-puro-T2A-BFP 3XUAS-mTK-mCherry
                     (adopted Gilbert 2014)"
                     /Transferred_Similarity="100.00%"
                     /modified_by="noviello"
                     /label="bla promoter"
     promoter        1433..1754
                     /gene="5' PB"
                     /note="5' PB repeat; PROMOTER 5' PB repeat"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /X-Transferred_From="Digestion of pSLQ2812_pPB:
                     CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9 
                     (modified) - Fragment 2; pSLQ2812_pPB:
                     CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9 
                     (modified); pSLQ2817_pPB:
                     CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
                     (modified); pSLQ2818_pPB:
                     CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA
                     PGK-ABI-tagBFP-SpdCas9;
                     pSLQ2833_CAG-PYL1-VPR-IRES-Zeocin-WPRE-SV40PA
                     PGK-ABI-tagBFP-SadCas9 (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pSLPB-EF1-MCS, pSLQ2812_pPB:
                     CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9 
                     (modified), pSLQ2817_pPB:
                     CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
                     (modified), pSLQ2818_pPB:
                     CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
                     , pSLQ2833_CAG-PYL1-VPR-IRES-Zeocin-WPRE-SV40PA
                     PGK-ABI-tagBFP-SadCas9 (modified)"
                     /Transferred_Similarity="100.00%"
                     /label="5' PB"
     promoter        1761..2306
                     /X-Transferred_From="Digestion of
                     pCDH-EF1a-MCS-pA-Pgk-copGFP-T2A-Puro (modified); PB-EF1a
                     empty; PiggyBac-EF1a empty; PiggyBac-EF1a-AID
                     FL-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     PiggyBac-EF1a-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     PiggyBac-EF1a-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     PiggyBac-EF1a-dCas9-tagRFPt-T2A-tagBFP;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-Venus;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P; pCDH-CMV-MCS-EF1-Puro; pCDH-EF1-MCS-CAG-MCS-T2A-Blast
                     v2; pCDH-EF1-MCS-PGK-Blast;
                     pCDH-EF1-MCS-PGK-attP1-Blast-attP2 v2;
                     pCDH-EF1-MCS-Pgk-Blast;
                     pCDH-EF1a-MCS-pA-Pgk-copGFP-T2A-Puro;
                     pCDH-EF1a-MCS-pA-Pgk-copGFP-T2A-Puro (modified);
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-Venus; pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFP-T; pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFP-T v2; pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-Venus; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFP-T; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt v2; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     pCDH550-AID(FL)-NLS-dCas9-NLS-VP192;
                     pCDH550-AID(FL)-linker-NLS-dCas9-His-NLS-VP192-pGK-Blast;
                     pCDH550-NLS-AID(FL)-dCas9-His-NLS-VP192-pGK-Blast;
                     pCDH550-NLS-AID(FL)-linker-dCas9-His-NLS-VP192-pGK-Blast;
                     pX335A_hCas9_D10A_"
                     /X-Transferred_Similarity="100.00%"
                     /vntifkey=29
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /Transferred_From="PiggyBac-EF1a empty, PiggyBac-EF1a-AID
                     FL-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-dCas9-tagRFPt-T2A-tagBFP,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P, pCDH-CMV-MCS-EF1-Puro, pCDH-EF1-MCS-CAG-MCS-T2A-Blast
                     v2, pCDH-EF1-MCS-PGK-Blast,
                     pCDH-EF1-MCS-PGK-attP1-Blast-attP2 v2,
                     pCDH-EF1a-MCS-pA-Pgk-copGFP-T2A-Puro, pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFP-T v2, pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP, pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt v2, pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     pCDH550-AID(FL)-linker-NLS-dCas9-His-NLS-VP192-pGK-Blast,
                     pCDH550-NLS-AID(FL)-dCas9-His-NLS-VP192-pGK-Blast,
                     pCDH550-NLS-AID(FL)-linker-dCas9-His-NLS-VP192-pGK-Blast,
                     pSLPB-EF1-MCS"
                     /Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /label="EF1a promoter"
     a               <2320..2336
                     /Transferred_From="RG77 mAID aa65-132 - 1 F A, RG80 ecDHFR
                     - 1 F A, RG83 FKBP DD - 1 F A"
                     /Transferred_Similarity="65.38%"
                     /label="Kozak+NotI"
     ORF             join(2334..>2364,<2842..>7819)
                     /Genetic_code="Standard"
                     /Reading_frame=3
                     /created_by="ORF Finder"
                     /label="ORF 7 (frame 3)"
     localization    2337..2357
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="pCAG-dCas9-5xPlat2AflD; pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFP-T v2; pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt v2"
                     /X-Transferred_Similarity="100.00%; 100.00% -> 90.48%;
                     90.48%"
                     /Transferred_From="pCDH-AID-linker-NLS-dCas9-NLS-His-linke
                     r-tagRFPt-T2A-tagBFP, pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFP-T v2, pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP, pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt v2"
                     /Transferred_Similarity="100.00%"
                     /label="NLS"
     CDS             2358..2846
                     /created_by="noviello"
                     /label="ecDHFR"
     misc_feature    <2361..2366
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    2841..>2845
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     polylinker      2847..2891
                     /created_by="User"
                     /Transferred_From="pCDH-AID-linker-NLS-dCas9-NLS-His-linke
                     r-tagRFPt-T2A-tagBFP"
                     /Transferred_Similarity="100.00%"
                     /label="GGS linker"
     gene            2892..6992
                     /created_by="User"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /X-Transferred_From="2014); Ligated sequences;
                     SV40-dCas9-10xGCN4_v4-T2A-Blasticidin;
                     pAC154-dual-dCas9VP160-sgExpression; pCAG-dCas9TET1CD;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-Venus;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P; pCDH-NLS-AID FL-dCas9-NLS-His-linker-Venus;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-Venus;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt v2;
                     pCDH550-AID(FL)-linker-NLS-dCas9-His-NLS-VP192-pGK-Blast;
                     pCDH550-NLS-AID(FL)-dCas9-His-NLS-VP192-pGK-Blast;
                     pCDH550-NLS-AID(FL)-linker-dCas9-His-NLS-VP192-pGK-Blast;
                     pEGIF dCas9-10XGCN4-blastR;
                     pHR-EF1a-KRAB-dCas9-P2A-mCherry;
                     pHR-PGK-KRAB-dCas9-P2A-mCherry;
                     pHR-SFFV-KRAB-dCas9-P2A-mCherry (Gilbert et al.;
                     pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP; pLenti-EF1a-dCAS9-VP64"
                     /X-Transferred_Similarity="100.00%; 100.00% -> 99.05%"
                     /Transferred_From="pCDH-AID-linker-NLS-dCas9-NLS-His-linke
                     r-tagRFP-T v2,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P, pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T v2,
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt v2,
                     pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP"
                     /Transferred_Similarity="100.00%"
                     /modified_by="noviello"
                     /label="pCas9(D10A, N863A)"
     localization    6993..7013
                     /created_by="User"
                     /X-Transferred_From="pAC154-dual-dCas9VP160-sgExpression;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-Venus;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P; pCDH-NLS-AID FL-dCas9-NLS-His-linker-Venus;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-Venus;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt v2;
                     pCDH550-AID(FL)-NLS-dCas9-NLS-VP192;
                     pCDH550-AID(FL)-linker-NLS-dCas9-His-NLS-VP192-pGK-Blast;
                     pCDH550-NLS-AID(FL)-dCas9-His-NLS-VP192-pGK-Blast;
                     pCDH550-NLS-AID(FL)-linker-dCas9-His-NLS-VP192-pGK-Blast"
                     /X-Transferred_Similarity="100.00%; 100.00% -> 90.48%;
                     90.48%"
                     /Transferred_From="pCDH-AID-linker-NLS-dCas9-NLS-His-linke
                     r-Venus,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P, pCDH-NLS-AID FL-dCas9-NLS-His-linker-Venus,
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T,
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T v2,
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-Venus,
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt,
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt v2,
                     pCDH550-AID(FL)-linker-NLS-dCas9-His-NLS-VP192-pGK-Blast,
                     pCDH550-NLS-AID(FL)-dCas9-His-NLS-VP192-pGK-Blast,
                     pCDH550-NLS-AID(FL)-linker-dCas9-His-NLS-VP192-pGK-Blast"
                     /Transferred_Similarity="100.00%"
                     /label="NLS"
     tag             7014..7031
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="pCDH-AID-linker-NLS-dCas9-NLS-His-lin
                     ker-Venus;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P; pCDH-NLS-AID FL-dCas9-NLS-His-linker-Venus;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-Venus;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt v2;
                     pCDH550-AID(FL)-NLS-dCas9-NLS-VP192;
                     pCDH550-AID(FL)-linker-NLS-dCas9-His-NLS-VP192-pGK-Blast;
                     pCDH550-NLS-AID(FL)-dCas9-His-NLS-VP192-pGK-Blast;
                     pCDH550-NLS-AID(FL)-linker-dCas9-His-NLS-VP192-pGK-Blast"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pCDH-AID-linker-NLS-dCas9-NLS-His-linke
                     r-Venus,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P, pCDH-NLS-AID FL-dCas9-NLS-His-linker-Venus,
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T,
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T v2,
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-Venus,
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt,
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt v2,
                     pCDH550-AID(FL)-linker-NLS-dCas9-His-NLS-VP192-pGK-Blast,
                     pCDH550-NLS-AID(FL)-dCas9-His-NLS-VP192-pGK-Blast,
                     pCDH550-NLS-AID(FL)-linker-dCas9-His-NLS-VP192-pGK-Blast"
                     /Transferred_Similarity="100.00%"
                     /label="6xHis tag"
     polylinker      7032..7076
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /modified_by="User"
                     /X-Transferred_From="pCDH-AID-linker-NLS-dCas9-NLS-His-lin
                     ker-Venus;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P; pCDH-NLS-AID FL-dCas9-NLS-His-linker-Venus;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-Venus;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt v2;
                     pCDH550-AID(FL)-NLS-dCas9-NLS-VP192;
                     pCDH550-AID(FL)-linker-NLS-dCas9-His-NLS-VP192-pGK-Blast;
                     pCDH550-NLS-AID(FL)-dCas9-His-NLS-VP192-pGK-Blast;
                     pCDH550-NLS-AID(FL)-linker-dCas9-His-NLS-VP192-pGK-Blast;
                     plenti-EF1a-dCAS9-VP64"
                     /X-Transferred_Similarity="100.00%; 93.75%; 95.83%"
                     /Transferred_From="pCDH-AID-linker-NLS-dCas9-NLS-His-linke
                     r-Venus,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P, pCDH-NLS-AID FL-dCas9-NLS-His-linker-Venus,
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T,
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T v2,
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-Venus,
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt,
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt v2,
                     pCDH550-AID(FL)-linker-NLS-dCas9-His-NLS-VP192-pGK-Blast,
                     pCDH550-NLS-AID(FL)-dCas9-His-NLS-VP192-pGK-Blast,
                     pCDH550-NLS-AID(FL)-linker-dCas9-His-NLS-VP192-pGK-Blast"
                     /Transferred_Similarity="100.00%"
                     /label="Linker (GGS)5"
     reporter        7086..7814
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /X-Transferred_From="pCDH-AID-linker-NLS-dCas9-NLS-His-lin
                     ker-tagRFP-T;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P; pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt v2;
                     pLL7.0-mTiam-tgRFPt-SspB (R73Q); pLL7.0-tgRFPt-SSPB R73Q
                     (iLID micro); pLL7.0-tgRFPt-SSPB WT (iLID nano)"
                     /X-Transferred_Similarity="100.00%; 99.86% -> 99.59%"
                     /Transferred_From="pCDH-AID-linker-NLS-dCas9-NLS-His-linke
                     r-tagRFP-T,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P, pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T,
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T v2,
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt,
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt v2"
                     /Transferred_Similarity="100.00%"
                     /modified_by="noviello"
                     /label="tagRFPt"
     ORF             <7818..8591
                     /Genetic_code="Standard"
                     /Reading_frame=2
                     /created_by="ORF Finder"
                     /label="ORF 16 (frame 2)"
     site            7824..7889
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="pSLPB-AID-dCas9-RFP-T2A-BFP-pGK-Blast
                     icidin-P2A-osTIR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pSLPB-AID-NLS-linker-dCas9-tagRFPt-tagP
                     2A-BFP-pGK-Blastidicin-T2A-osTIR"
                     /Transferred_Similarity="100.00%"
                     /label="P2A"
     reporter        7890..8588
                     /SerialCloner_Color="&h1D7D29"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#eb1439"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /X-Transferred_From="Digestion of
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P - Fragment 1; Digestion of
                     pSLLV1-EF1-dCas9-tagRFPt-2A-tagBFP - Fragment 2;
                     PiggyBac-EF1a-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     PiggyBac-EF1a-dCas9-tagRFPt-T2A-tagBFP;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P; pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     pSLLV1-dCas9-VP192-T2A-tagBFP;
                     pSLLV1-dCas9-VP64-T2A-tagBFP;
                     pSLLV1-dCas9-tagRFPt-T2A-tagBFP;
                     pSLPB-AID-NLS-linker-dCas9-tagRFP-T2A-tagBFP;
                     pSLPB-AID-dCas9-RFP-T2A-BFP-pGK-Blasticidin-P2A-osTIR;
                     pSLQ2812_pPB: CAG-GID1-VPR-IRES-Puro-WPRE
                     PGK-GAI-tagBFP-SpdCas9  (modified); pSLQ2817_pPB:
                     CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
                     (modified); pSLQ2818_pPB:
                     CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA
                     PGK-ABI-tagBFP-SpdCas9;
                     pSLQ2833_CAG-PYL1-VPR-IRES-Zeocin-WPRE-SV40PA
                     PGK-ABI-tagBFP-SadCas9 (modified)"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="User"
                     /Transferred_From="Digestion of
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P - Fragment 1, Digestion of
                     pSLLV1-EF1-dCas9-tagRFPt-2A-tagBFP - Fragment 2,
                     PiggyBac-EF1a-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-dCas9-tagRFPt-T2A-tagBFP,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P, pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     pSLLV1-dCas9-VP192-T2A-tagBFP,
                     pSLLV1-dCas9-VP64-T2A-tagBFP,
                     pSLLV1-dCas9-tagRFPt-T2A-tagBFP,
                     pSLPB-AID-NLS-linker-dCas9-tagRFPt-T2A-tagBFP-pGK-Blastici
                     din,
                     pSLPB-AID-NLS-linker-dCas9-tagRFPt-tagP2A-BFP-pGK-Blastidi
                     cin-T2A-osTIR"
                     /Transferred_Similarity="100.00%"
                     /label="tagBFP"
     terminator      8603..8830
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /SerialCloner_Color="&hFF8000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="False"
                     /SerialCloner_Arrow="True"
                     /vntifkey=43
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /X-Transferred_From="Digestion of
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P - Fragment 1; Digestion of
                     pCDH-EF1a-MCS-pA-Pgk-copGFP-T2A-Puro (modified); Digestion
                     of pSLLV1-EF1-dCas9-tagRFPt-2A-tagBFP - Fragment 2;
                     Digestion of pcDNA3.1_pMagFast2(3x)-iRFP 2 - Fragment 2;
                     PB-TET-mOct4; PX855 (dCas9(N)-FRB-2xNES);
                     PiggyBac-EF1a-AID
                     FL-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     PiggyBac-EF1a-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     PiggyBac-EF1a-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     PiggyBac-EF1a-dCas9-tagRFPt-T2A-tagBFP; bGH_PA term;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-Venus;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P; pCDH-EF1-MCS-CAG-MCS-T2A-Blast v2;
                     pCDH-EF1-MCS-PGK-Blast; pCDH-EF1-MCS-PGK-attP1-Blast-attP2
                     v2; pCDH-EF1a-MCS-pA-Pgk-copGFP-T2A-Puro;
                     pCDH-EF1a-MCS-pA-Pgk-copGFP-T2A-Puro (modified);
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-Venus; pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFP-T; pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFP-T v2; pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-Venus; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFP-T; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt v2; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     pSLLV1-dCas9-VP192-T2A-tagBFP;
                     pSLLV1-dCas9-VP64-T2A-tagBFP;
                     pSLLV1-dCas9-tagRFPt-T2A-tagBFP;
                     pSLPB-AID-dCas9-RFP-T2A-BFP-pGK-Blasticidin-P2A-osTIR;
                     pcDNA3.1_nMagHigh1-EGFP-CAAX 2;
                     pcDNA3.1_pMagFast2(3x)-iRFP 2; pcDNA5-H2B-AID-EYFP"
                     /X-Transferred_Similarity="100.00%; 100.00% -> 92.98%"
                     /Transferred_From="Digestion of
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P - Fragment 1, Digestion of
                     pSLLV1-EF1-dCas9-tagRFPt-2A-tagBFP - Fragment 2,
                     PiggyBac-EF1a-AID
                     FL-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-dCas9-tagRFPt-T2A-tagBFP,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P, pCDH-EF1-MCS-PGK-attP1-Blast-attP2 v2,
                     pCDH-EF1a-MCS-pA-Pgk-copGFP-T2A-Puro, pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFP-T v2, pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP, pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt v2, pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     pSLLV1-dCas9-VP192-T2A-tagBFP,
                     pSLLV1-dCas9-VP64-T2A-tagBFP,
                     pSLLV1-dCas9-tagRFPt-T2A-tagBFP,
                     pSLPB-AID-NLS-linker-dCas9-tagRFPt-tagP2A-BFP-pGK-Blastidi
                     cin-T2A-osTIR, pcDNA3.1_nMagHigh1-EGFP-CAAX 2,
                     pcDNA3.1_pMagFast2(3x)-iRFP 2, pcDNA5-H2B-AID-EYFP "
                     /Transferred_Similarity="100.00%"
                     /modified_by="noviello"
                     /label="bGH_PA_terminator"
     promoter        8831..9255
                     /modified_by="User"
                     /X-Transferred_From="Digestion of
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P - Fragment 1; Digestion of
                     pCDH-EF1a-MCS-pA-Pgk-copGFP-T2A-Puro (modified); Digestion
                     of pSLLV1-EF1-dCas9-tagRFPt-2A-tagBFP - Fragment 2;
                     PiggyBac-EF1a-AID
                     FL-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     PiggyBac-EF1a-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     PiggyBac-EF1a-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     PiggyBac-EF1a-dCas9-tagRFPt-T2A-tagBFP;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-Venus;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P; pCDH-EF1-MCS-PGK-Blast;
                     pCDH-EF1-MCS-PGK-attP1-Blast-attP2 v2;
                     pCDH-EF1a-MCS-pA-Pgk-copGFP-T2A-Puro;
                     pCDH-EF1a-MCS-pA-Pgk-copGFP-T2A-Puro (modified);
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-Venus; pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFP-T; pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFP-T v2; pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-Venus; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFP-T; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt v2; pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     pCDH550-AID(FL)-NLS-dCas9-NLS-VP192;
                     pCDH550-AID(FL)-linker-NLS-dCas9-His-NLS-VP192-pGK-Blast;
                     pCDH550-NLS-AID(FL)-dCas9-His-NLS-VP192-pGK-Blast;
                     pCDH550-NLS-AID(FL)-linker-dCas9-His-NLS-VP192-pGK-Blast;
                     pSLLV1-MCS-PGK-Blast; pSLLV1-dCas9-VP192-T2A-tagBFP;
                     pSLLV1-dCas9-VP64-T2A-tagBFP;
                     pSLLV1-dCas9-tagRFPt-T2A-tagBFP;
                     pSLPB-AID-NLS-linker-dCas9-tagRFP-T2A-tagBFP;
                     pSLPB-AID-dCas9-RFP-T2A-BFP-pGK-Blasticidin-P2A-osTIR;
                     pSUPER-EF1-Puro-U6-sgRNA-TetO-mCMV-mCherry;
                     pSUPER-EF1-Puro-U6-sgRNA-UAS-mTK-mCherry"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P - Fragment 1, Digestion of
                     pSLLV1-EF1-dCas9-tagRFPt-2A-tagBFP - Fragment 2,
                     PiggyBac-EF1a-AID
                     FL-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-dCas9-tagRFPt-T2A-tagBFP,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P, pCDH-EF1-MCS-PGK-attP1-Blast-attP2 v2,
                     pCDH-EF1a-MCS-pA-Pgk-copGFP-T2A-Puro, pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFP-T v2, pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP, pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt v2, pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     pCDH550-AID(FL)-linker-NLS-dCas9-His-NLS-VP192-pGK-Blast,
                     pCDH550-NLS-AID(FL)-dCas9-His-NLS-VP192-pGK-Blast,
                     pCDH550-NLS-AID(FL)-linker-dCas9-His-NLS-VP192-pGK-Blast,
                     pSLLV1-MCS-PGK-Blast, pSLLV1-dCas9-VP192-T2A-tagBFP,
                     pSLLV1-dCas9-VP64-T2A-tagBFP,
                     pSLLV1-dCas9-tagRFPt-T2A-tagBFP,
                     pSLPB-AID-NLS-linker-dCas9-tagRFPt-T2A-tagBFP-pGK-Blastici
                     din,
                     pSLPB-AID-NLS-linker-dCas9-tagRFPt-tagP2A-BFP-pGK-Blastidi
                     cin-T2A-osTIR, pSUPER-EF1-Puro-U6-sgRNA-TetO-mCMV-mCherry,
                     pSUPER-EF1-Puro-U6-sgRNA-UAS-mTK-mCherry"
                     /Transferred_Similarity="100.00%"
                     /label="PGK"
     cds             9256..9654
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /modified_by="User"
                     /X-Transferred_From="Digestion of PiggyBac-EF1a-AID
                     FL-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     Digestion of pLenti-EF1a-dCAS9-VP64 PCR Product - Fragment
                     2; Digestion of pLenti-EF1a-dCas9-VP192; Digestion of
                     pLenti-EF1a-dCas9-VP64; Ligated sequences;
                     PiggyBac-EF1a-AID
                     FL-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     PiggyBac-EF1a-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     PiggyBac-EF1a-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     PiggyBac-EF1a-dCas9-tagRFPt-T2A-tagBFP;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-Venus;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P; pCDH-EF1-MCS-CAG-MCS-T2A-Blast v2;
                     pCDH-EF1-MCS-PGK-Blast; pCDH-EF1-MCS-PGK-attP1-Blast-attP2
                     v2; pCDH-NLS-AID FL-dCas9-NLS-His-linker-Venus;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-Venus;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFP-T;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt v2;
                     pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     pCDH550-AID(FL)-NLS-dCas9-NLS-VP192;
                     pCDH550-AID(FL)-linker-NLS-dCas9-His-NLS-VP192-pGK-Blast;
                     pCDH550-NLS-AID(FL)-dCas9-His-NLS-VP192-pGK-Blast;
                     pCDH550-NLS-AID(FL)-linker-dCas9-His-NLS-VP192-pGK-Blast;
                     pLenti-EF1-dCas9-SVPR; pLenti-EF1-dCas9-VPR;
                     pLenti-EF1a-dCAS9-VP64; pLenti-EF1a-dCAS9-VP64 PCR
                     Product; pLenti-EF1a-dCas9-VP192; pLenti-EF1a-dCas9-VP64;
                     pSLLV1-EF1-MCS-PGK-Blast;
                     pSLLV1-EF1-dCas9-tagRFPt-2A-tagBFP;
                     plenti-EF1a-dCAS9-VP64"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="Digestion of
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P - Fragment 1, Digestion of
                     pSLLV1-EF1-dCas9-tagRFPt-2A-tagBFP - Fragment 2,
                     PiggyBac-EF1a-AID
                     FL-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-dCas9-tagRFPt-T2A-tagBFP,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFP-T v2,
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P, pCDH-EF1-MCS-PGK-attP1-Blast-attP2 v2, pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFP-T v2, pCDH-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP, pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt v2, pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     pCDH550-AID(FL)-linker-NLS-dCas9-His-NLS-VP192-pGK-Blast,
                     pCDH550-NLS-AID(FL)-dCas9-His-NLS-VP192-pGK-Blast,
                     pCDH550-NLS-AID(FL)-linker-dCas9-His-NLS-VP192-pGK-Blast,
                     pLenti-EF1a-dCas9-SVPR, pLenti-EF1a-dCas9-VP192,
                     pLenti-EF1a-dCas9-VP64, pLenti-EF1a-dCas9-VPR,
                     pSLLV1-MCS-PGK-Blast, pSLLV1-dCas9-VP192-T2A-tagBFP,
                     pSLLV1-dCas9-VP64-T2A-tagBFP,
                     pSLLV1-dCas9-tagRFPt-T2A-tagBFP,
                     pSLLV2-MCS-CAG-MCS-T2A-Blast,
                     pSLPB-AID-NLS-linker-dCas9-tagRFPt-T2A-tagBFP-pGK-Blastici
                     din"
                     /Transferred_Similarity="100.00%"
                     /label="Blasticidin"
     terminator      9655..10244
                     /X-Transferred_From="pCDH-AID-linker-NLS-dCas9-NLS-His-lin
                     ker-Venus;
                     pCDH-AID-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBF
                     P; pCDH-EF1-MCS-PGK-Blast; pCDH-EF1-MCS-Pgk-Blast;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFP-T v2;
                     pCDH-NLS-AID FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-Venus;
                     pCDH-NLS-AID FL-linker-dCas9-NLS-His-linker-tagRFPt v2;
                     pCDH-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP;
                     pLL7.0-Venus-iLID-CAAX; pSLLV1-MCS-PGK-Blast;
                     pSLLV1-dCas9-tagRFPt-T2A-tagBFP;
                     pSLPB-AID-NLS-linker-dCas9-tagRFP-T2A-tagBFP;
                     pSLPB-AID-dCas9-RFP-T2A-BFP-pGK-Blasticidin-P2A-osTIR"
                     /X-Transferred_Similarity="100.00%; 99.16%"
                     /modified_by="User"
                     /Transferred_From="pSLLV1-MCS-PGK-Blast,
                     pSLLV1-dCas9-tagRFPt-T2A-tagBFP,
                     pSLPB-AID-NLS-linker-dCas9-tagRFPt-T2A-tagBFP-pGK-Blastici
                     din,
                     pSLPB-AID-NLS-linker-dCas9-tagRFPt-tagP2A-BFP-pGK-Blastidi
                     cin-T2A-osTIR"
                     /Transferred_Similarity="100.00%"
                     /label="WPRE"
     promoter        10251..10498
                     /gene="3' PB"
                     /note="3' PB repeat; PROMOTER 3' PB repeat"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pSLQ2812_pPB:
                     CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9 
                     (modified), pSLQ2817_pPB:
                     CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
                     (modified), pSLQ2818_pPB:
                     CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
                     , pSLQ2833_CAG-PYL1-VPR-IRES-Zeocin-WPRE-SV40PA
                     PGK-ABI-tagBFP-SadCas9 (modified)"
                     /Transferred_Similarity="100.00%"
                     /label="3' PB"
     gene            10777..11717
                     /created_by="noviello"
                     /label="ColE1 origin gene"
     CDS             complement(11718..649)
                     /gene="AmpR; amp; bla"
                     /note="AmpR; CDS beta-lactamase AmpR confers resistance to
                     ampici llin, carbenicillin, and related antibiotics; This
                     feature has 2 segments:  1: 4470 .. 5261 / #ccffcc 2: 5262
                     .. 5330 / #ccffcc / signal sequence Cleavage site after
                     base 5261; ampR (confers resistance to ampicillin);
                     confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /codon_start=1
                     /product="ampicillin resistance protein; beta-lactamase"
                     /Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVK
                     DAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLG
                     RRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTA
                     FLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLI
                     DWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGS
                     QATMDERNRQIAEIGAS LIKHW"
                     /ApEinfo_fwdcolor="#e1e100; pink"
                     /ApEinfo_revcolor="#e8e800; pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0; arrow_data {{0 1 2 0 0 -1} {} 0}width 5
                     offset 0"
                     /transl_table=11
                     /protein_id="BAF36708.1"
                     /db_xref="GI:117939119"
                     /X-Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVK
                     VKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQ
                     LGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKEL
                     TAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQ
                     LIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTT
                     GSQATMDERNRQIAEIGAS LIKHW;
                     MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESF
                     RPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMT
                     VRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPND
                     ERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADK
                     SGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
                     /vntifkey=4
                     /dnas_title="AmpR"
                     /cds_type="ORF"
                     /invitrogen="""1020000"
                     /X-Transferred_From="302; Clipboard Contents 3 2;
                     Fire_reporter; Fire_reporter_ba_mVenus; LSL-Cas9-Rosa26TV
                     Sequences; PB-empty_GFP-BSDR; PN_186_PB-TRE-SunTag;
                     SV40-dCas9-10xGCN4_v4-T2A-Blasticidin; lentiCas9-Blast;
                     lentiGuide-Puro; pBROAD3-PCP-EGFP-NLS_Abhi;
                     pBROAD3-TetR-ICP; pCAGG-L/eGFP; pCAGGS-Li; pCR4-12xMBS-PBS
                     addgene; pDN-D2irTNG4kwh; pEGIF dCas9-10XGCN4-blastR;
                     pEGIP; pEGIP with AscI; pSLQ2812_pPB:
                     CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9 
                     (modified); pSLQ2814_PiggyBa; pSLQ2817_pPB:
                     CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
                     (modified); pSLQ2818_PiggyBa (modified); pSLQ2818_pPB:
                     CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA
                     PGK-ABI-tagBFP-SpdCas9;
                     pSLQ2833_CAG-PYL1-VPR-IRES-Zeocin-WPRE-SV40PA
                     PGK-ABI-tagBFP-SadCas9 (modified); pTRETightBI-RY-0;
                     pUC19; pUC19_dig_XbaI; pUC19_g1_EGFP_Neo;
                     pUC19_g1_mCherry_Puro; pUC19_g2_eGFP_neo;
                     pUC19_g2_mCherry_puro; pUC19_g4_EGFP_Neo;
                     pUC19_g4_mC_puro; pUC19_g5_EGFP_Neo;
                     pUC19_g5_mCherry_Puro; pUC19_g6_EGFP_puro;
                     pUC19_g6_mCherry_puro; pUC19_g7_EGFP_puro;
                     pUC19_g7_mCherry_puro"
                     /X-Transferred_Similarity="100.00%; 100.00% -> 99.88%"
                     /Transferred_From="PiggyBac-EF1a empty, PiggyBac-EF1a-AID
                     FL-linker-NLS-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-NLS-AID
                     FL-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-NLS-AID
                     FL-linker-dCas9-NLS-His-linker-tagRFPt-T2A-tagBFP,
                     PiggyBac-EF1a-dCas9-tagRFPt-T2A-tagBFP,
                     PiggyBac-empty_GFP-BSDR, lentiGuide-Puro,
                     pLL7.0-mTiam-tgRFPt-SspB (R73Q), pSLQ2812_pPB:
                     CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9 
                     (modified), pSLQ2817_pPB:
                     CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
                     (modified), pSLQ2818_pPB:
                     CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9
                     , pSLQ2833_CAG-PYL1-VPR-IRES-Zeocin-WPRE-SV40PA
                     PGK-ABI-tagBFP-SadCas9 (modified)"
                     /Transferred_Similarity="100.00%"
                     /Color="#ff0000"
                     /modified_by="noviello"
                     /label="AmpR"
ORIGIN      
        1 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
       61 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
      121 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
      181 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
      241 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
      301 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
      361 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
      421 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
      481 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
      541 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
      601 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
      661 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
      721 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
      781 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
      841 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
      901 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
      961 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     1021 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     1081 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     1141 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     1201 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa
     1261 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     1321 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     1381 aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg ggttaaccct
     1441 agaaagatag tctgcgtaaa attgacgcat gcattcttga aatattgctc tctctttcta
     1501 aatagcgcga atccgtcgct gtgcatttag gacatctcag tcgccgcttg gagctcccgt
     1561 gaggcgtgct tgtcaatgcg gtaagtgtca ctgattttga actataacga ccgcgtgagt
     1621 caaaatgacg catgattatc ttttacgtga cttttaagat ttaactcata cgataattat
     1681 attgttattt catgttctac ttacgtgata acttattata tatatatttt cttgttatag
     1741 atatcaacta gaatacgcgt aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     1801 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     1861 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     1921 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     1981 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     2041 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     2101 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     2161 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     2221 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     2281 agatccaagc tgtgaccggc gcctactcta gagctagcga attcgccgcc accatgccta
     2341 agaaaaagag gaaggtgagc ggccgcatca gtctgattgc ggcgttagcg gtagattacg
     2401 ttatcggcat ggaaaacgcc atgccgtgga acctgcctgc cgatctcgcc tggtttaaac
     2461 gcaacacctt aaataaaccc gtgattatgg gccgccatac ctgggaatca atcggtcgtc
     2521 cgttgccagg acgcaaaaat attatcctca gcagtcaacc gagtacggac gatcgcgtaa
     2581 cgtgggtgaa gtcggtggat gaagccatcg cggcgtgtgg tgacgtacca gaaatcatgg
     2641 tgattggcgg cggtcgcgtt attgaacagt tcttgccaaa agcgcaaaaa ctgtatctga
     2701 cgcatatcga cgcagaagtg gaaggcgaca cccatttccc ggattacgag ccggatgact
     2761 gggaatcggt attcagcgaa ttccacgatg ctgatgcgca gaactctcac agctattgct
     2821 ttgagattct ggagcggcga ggatccggcg gatctggagg tagtggtgga tctggaggtt
     2881 ctggtggctc cgacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct
     2941 gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca
     3001 ccgaccggca cagcatcaag aagaacctga tcggcgccct gctgttcgac agcggagaaa
     3061 cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc
     3121 ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct
     3181 tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca
     3241 tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc
     3301 tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc
     3361 tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca
     3421 acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg
     3481 aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca
     3541 agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt
     3601 tcggcaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc
     3661 tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc
     3721 tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg
     3781 acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga
     3841 gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc
     3901 tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg
     3961 gctacgccgg ctacatcgat ggcggagcca gccaggaaga gttctacaag ttcatcaagc
     4021 ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc
     4081 tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag
     4141 agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg
     4201 aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg
     4261 gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact
     4321 tcgaggaagt ggtggacaag ggcgccagcg cccagagctt catcgagcgg atgaccaact
     4381 tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact
     4441 tcaccgtgta caacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg
     4501 ccttcctgag cggcgagcag aaaaaagcca tcgtggacct gctgttcaag accaaccgga
     4561 aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg
     4621 tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc
     4681 tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag
     4741 atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa
     4801 cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg
     4861 gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga
     4921 caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc
     4981 acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg
     5041 atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc
     5101 tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga
     5161 acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc
     5221 gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag
     5281 aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga
     5341 atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg
     5401 tggacgctat cgtgcctcag agctttctga aggacgactc catcgataac aaagtgctga
     5461 ctcggagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga
     5521 agatgaagaa ctactggcgc cagctgctga atgccaagct gattacccag aggaagttcg
     5581 acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca
     5641 agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc
     5701 ggatgaacac taagtacgac gagaacgaca aactgatccg ggaagtgaaa gtgatcaccc
     5761 tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga
     5821 tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga
     5881 tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg
     5941 tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct
     6001 tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc
     6061 ggaagcggcc tctgatcgag acaaacggcg aaacaggcga gatcgtgtgg gataagggcc
     6121 gggactttgc caccgtgcgg aaagtgctgt ctatgcccca agtgaatatc gtgaaaaaga
     6181 ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgaca
     6241 agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca
     6301 ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga
     6361 agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc
     6421 ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc
     6481 tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg
     6541 gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc
     6601 tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt
     6661 ttgtggaaca gcacaaacac tacctggacg agatcatcga gcagatcagc gagttctcca
     6721 agagagtgat cctggccgac gctaatctgg acaaggtgct gagcgcctac aacaagcaca
     6781 gagacaagcc tatcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc
     6841 tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca
     6901 gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga
     6961 cacggatcga cctgtctcag ctgggaggcg accccaagaa gaagcgcaag gtgcaccacc
     7021 accaccacca cggaggaggt ggaagcggag gaggaggaag cggaggagga ggtagctggc
     7081 gcgccgtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag ctgtacatgg
     7141 agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg
     7201 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
     7261 acatcctggc taccagcttc atgtacggca gcagaacctt catcaaccac acccagggca
     7321 tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
     7381 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
     7441 tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga
     7501 aaacactcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc ggcctggaag
     7561 gcagaaccga catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttcaaga
     7621 ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg
     7681 accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgagg
     7741 tggctgtggc cagatactgc gacctcccta gcaaactggg gcacaaactt aatggcatgg
     7801 acgagctgta caagttaatt aacggaagcg gagctactaa cttcagcctg ctgaagcagg
     7861 ctggagacgt ggaggagaac cctggaccta tgagcgagct gattaaggag aacatgcaca
     7921 tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg
     7981 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc
     8041 tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca
     8101 accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg
     8161 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
     8221 aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc
     8281 ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg taccccgctg
     8341 acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga
     8401 tcgcaaacat caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg
     8461 gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg
     8521 tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca
     8581 agcttaatta gacgcgtatt aacgactgtg ccttctagtt gccagccatc tgttgtttgc
     8641 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     8701 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     8761 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     8821 ggctctatgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     8881 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     8941 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     9001 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     9061 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     9121 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     9181 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     9241 gcctttcgac ctgcaatggc caagcctttg tctcaagaag aatccaccct cattgaaaga
     9301 gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct
     9361 ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct
     9421 tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt
     9481 atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag
     9541 gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg
     9601 acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaaaatcaa
     9661 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     9721 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     9781 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     9841 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     9901 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     9961 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
    10021 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
    10081 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
    10141 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt
    10201 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgccggtacc taaaagtttt
    10261 gttactttat agaagaaatt ttgagttttt gttttttttt aataaataaa taaacataaa
    10321 taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa acttaatatc
    10381 tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc gtcaatttta
    10441 cgcatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt aatctagtat
    10501 taatccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
    10561 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
    10621 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
    10681 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
    10741 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    10801 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    10861 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    10921 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    10981 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    11041 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    11101 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    11161 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    11221 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    11281 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    11341 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
    11401 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    11461 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    11521 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    11581 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    11641 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    11701 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    11761 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    11821 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    11881 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtg
//
