LOCUS       Exported                6229 bp DNA     circular SYN 16-JUN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 20, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6229
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      1..141
                     /direction=RIGHT
                     /label=ITR
                     /note="color: #ffff00"
     promoter        230..921
                     /label=GFAP(0.7Kb)
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      921..937
                     /direction=RIGHT
                     /label=MCS
                     /note="color: #ffff00"
     CDS             943..2394
                     /codon_start=1
                     /label=ArcLight
                     /note="color: #993366"
                     /translation="MEGFDGSDFSPPADLVGVDGAVMRNVVDVTINGDVTAPPKAAPRK
                     SESVKKVHWNDVDQGPSEKPETRQEERIDIPEISGLWWGENEHGVDDGRMEIPTTGVGR
                     VQFRVRAVIDHLGMRVFGVFLIFLDIILMIIDLSLPGKSESSQSFYDGMALALSCYFML
                     DLGLRIFAYGPKNFFTNPWEVADGLIIVVTFVVTIFYTVLDEYVQETGADGLGQLVVLA
                     RLLRVVRLARIFYSHQQMKAGDPMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGD
                     ATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQE
                     RTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMA
                     DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNE
                     KRDHMVLLEFVTADGITHGMDELYK"
     CDS             1678..2394
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /note="color: #993366"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTADGITHGMDELYK"
     primer_bind     complement(1708..1736)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     2331..2351
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    2523..3114
                     /label=WPRE
                     /note="color: #999999"
     rep_origin      3180..3407
                     /direction=RIGHT
                     /label=bGHpA
                     /note="color: #ffff00"
     rep_origin      3492..3632
                     /direction=RIGHT
                     /label=ITR
                     /note="color: #ffff00"
     rep_origin      4549..5406
                     /direction=RIGHT
                     /label=Amp(R)
                     /note="color: #ffff00"
     rep_origin      5557..6224
                     /direction=RIGHT
                     /label=pUCori
                     /note="color: #ffff00"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggcaatt cagtcgataa ctataacggt cctaaggtag
      181 cgatttaaat acgcgctctc ttaaggtagc cccgggacgc gtcaattgag aacatatcct
      241 ggtgtggagt aggggacgct gctctgacag aggctcgggg gcctgagctg gctctgtgag
      301 ctggggagga ggcagacagc caggccttgt ctgcaagcag acctggcagc attgggctgg
      361 ccgcccccca gggcctcctc ttcatgccca gtgaatgact caccttggca cagacacaat
      421 gttcggggtg ggcacagtgc ctgcttcccg ccgcacccca gcccccctca aatgccttcc
      481 gagaagccca ttgagcaggg ggcttgcatt gcaccccagc ctgacagcct ggcatcttgg
      541 gataaaagca gcacagcccc ctaggggctg cccttgctgt gtggcgccac cggcggtgga
      601 gaacaaggct ctattcagcc tgtgcccagg aaaggggatc aggggatgcc caggcatgga
      661 cagtgggtgg caggggggga gaggagggct gtctgcttcc cagaagtcca aggacacaaa
      721 tgggtgaggg gactggaggg gagctctccc catagctggg ctgcggccca accccacccc
      781 ctcaggctat gccagggggt gttgccaggg gcacccgggc atcgccagtc tagcccactc
      841 cttcataaag ccctcgcatc ccaggagcga gcagagccag agcaggttgg agaggagacg
      901 catcacctcc gctgctcgcc gctagcgttt aaacttaagc ttatggaggg attcgacggt
      961 tcagatttta gtcctccagc tgatttagtt ggcgttgacg gtgcagtcat gcggaacgtc
     1021 gttgacgtca cgataaatgg tgacgtcact gctccgccga aagctgcgcc aagaaaaagt
     1081 gaatcggtaa agaaagttca ttggaatgat gtagaccaag gaccgagtga aaaaccagag
     1141 acaagacagg aggaacgaat agatataccc gagatttcag gtctatggtg gggcgagaat
     1201 gaacatggag tggacgatgg gagaatggag atacctacta ctggtgtagg tcgcgtccag
     1261 tttcgtgtcc gagcagtgat tgatcatcta gggatgcgag tctttggagt cttcctaatt
     1321 ttcttggaca tcatcctcat gatcattgat ctcagtcttc caggaaaaag tgaatcttca
     1381 caatcctttt atgacgggat ggctttggct ctttcttgtt atttcatgct ggatttagga
     1441 ttaaggatat ttgcctacgg gcccaagaat ttcttcacca acccctggga ggttgctgat
     1501 ggtttgatta tcgttgtcac attcgtcgtc acgatatttt acactgtgtt agatgaatac
     1561 gtgcaagaaa caggagccga tggtttgggg caattggttg tgttggcccg tttgctgcgt
     1621 gtggttcgat tagcaagaat attttattcc caccaacaaa tgaaggctgg ggatcccatg
     1681 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     1741 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     1801 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
     1861 actactttaa cttatggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat
     1921 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     1981 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     2041 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
     2101 gaatacaact ataacgatca ccaggtgtac atcatggcag acaaacaaaa gaatggaatc
     2161 aaagctaact tcaaaattag acacaacatt gaagatggag gcgttcaact agcagaccat
     2221 tatcaacaaa atactccaat tggcgatggg cccgtccttt taccagacaa ccattacctg
     2281 tttacaactt ctactctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     2341 gagtttgtaa cagctgatgg gattacacat ggcatggatg aactatacaa atgatctaga
     2401 actatagtga gtcgtattac atccagcaca gtggcggccg ctcgagtcta gagggccctt
     2461 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggttaatc
     2521 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2581 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2641 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     2701 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     2761 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     2821 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     2881 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     2941 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3001 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3061 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgaaac
     3121 ccgctgatca gccggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
     3181 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     3241 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     3301 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     3361 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     3421 aagaaccaga tcctctctta aggtagcatc gagatttaaa ttagggataa cagggtaatg
     3481 gcgcgggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     3541 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     3601 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     3661 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     3721 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3781 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3841 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3901 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     3961 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4021 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     4081 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4141 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     4201 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     4261 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     4321 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     4381 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4441 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4501 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4561 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4621 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4681 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4741 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4801 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4861 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4921 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4981 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5041 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5101 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5161 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5221 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5281 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5341 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5401 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5461 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5521 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5581 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5641 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5701 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     5761 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5821 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5881 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5941 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6001 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6061 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6121 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6181 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
