LOCUS       Exported                6313 bp DNA     circular SYN 16-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jun 20, 2022 from SnapGene Viewer 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6313
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      1..141
                     /direction=RIGHT
                     /label=ITR
     promoter        230..921
                     /label=GFAP(0.7Kb)
     rep_origin      921..955
                     /direction=RIGHT
                     /label=MCS
     regulatory      962..971
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1775..2491
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1817..1838)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2078..2097)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2425..2446
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     rep_origin      2607..3198
                     /direction=RIGHT
                     /label=WPRE
     rep_origin      3264..3491
                     /direction=RIGHT
                     /label=bGHpA
     rep_origin      3576..3716
                     /direction=RIGHT
                     /label=ITR
     rep_origin      4633..5490
                     /direction=RIGHT
                     /label=Amp(R)
     rep_origin      5641..6308
                     /direction=RIGHT
                     /label=pUCori
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggcaatt cagtcgataa ctataacggt cctaaggtag
      181 cgatttaaat acgcgctctc ttaaggtagc cccgggacgc gtcaattgag aacatatcct
      241 ggtgtggagt aggggacgct gctctgacag aggctcgggg gcctgagctg gctctgtgag
      301 ctggggagga ggcagacagc caggccttgt ctgcaagcag acctggcagc attgggctgg
      361 ccgcccccca gggcctcctc ttcatgccca gtgaatgact caccttggca cagacacaat
      421 gttcggggtg ggcacagtgc ctgcttcccg ccgcacccca gcccccctca aatgccttcc
      481 gagaagccca ttgagcaggg ggcttgcatt gcaccccagc ctgacagcct ggcatcttgg
      541 gataaaagca gcacagcccc ctaggggctg cccttgctgt gtggcgccac cggcggtgga
      601 gaacaaggct ctattcagcc tgtgcccagg aaaggggatc aggggatgcc caggcatgga
      661 cagtgggtgg caggggggga gaggagggct gtctgcttcc cagaagtcca aggacacaaa
      721 tgggtgaggg gactggaggg gagctctccc catagctggg ctgcggccca accccacccc
      781 ctcaggctat gccagggggt gttgccaggg gcacccgggc atcgccagtc tagcccactc
      841 cttcataaag ccctcgcatc ccaggagcga gcagagccag agcaggttgg agaggagacg
      901 catcacctcc gctgctcgcc gctagcgttt aaacttaagc ttggtaccga gctcggatcc
      961 cgccaccatg gtttctatcg cactgcaggc tggttatgac ctcctcggag atggtagacc
     1021 tgaaagtctg tggctcggaa ttggtacact gctgatgctg atcggaacct tctacttcct
     1081 ggttagagcg tggggagaga ctgacaagga tgcacgggag tactacgcag tgacaatcct
     1141 ggtcagcggt attgccagtg cagcttatct gtcaatgttc tttggtattg gcctcacaga
     1201 ggtgccagta ggtggagaga tgctgaacat ctactacgca agatacgcac agtggctgtt
     1261 cactacacca ctgttgctgc tgcacctggc actcctggcc aaagttgata gagtgaccat
     1321 cggcactctc gttggcgtcg atgctctgat gattgtcaca ggcctgatag gaacactgtc
     1381 acatactgcc attgccagat actcttggtg gctgttctca actatctgta tgatcgtcgt
     1441 tctgtatgtc ctggccactt ctctgcggtc agccgctaag gagagaggcc cagaggttgc
     1501 ctcaaccttc aacatcctca ccgcactggt tctggtcctg tggactgctt accctatcat
     1561 ctggattatc ggcaccgagg gtgccggagt tgtgggcctg ggaatcgaaa ctttgctgtt
     1621 catggtgctg gacgtcaccg ccaaggtggg cttcggtttt atcctgctca ggtcaagagc
     1681 cattctgcaa gatacagaag cacctgaacc ttcagctgga gctgataccg gtgccgccga
     1741 ccgcccggta gtagcagtga gcaaggcggc cgccgtgagc aagggcgagg agctgttcac
     1801 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1861 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatttgcac
     1921 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     1981 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2041 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2101 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2161 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     2221 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     2281 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2341 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     2401 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2461 cactctcggc atggacgagc tgtacaagta agaattctgc agatatccag cacagtggcg
     2521 gccgctcgag tctagagggc ccttcgaagg taagcctatc cctaaccctc tcctcggtct
     2581 cgattctacg cgtaccggtt aatcgataat caacctctgg attacaaaat ttgtgaaaga
     2641 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2701 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2761 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2821 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2881 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2941 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3001 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3061 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3121 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3181 tgggccgcct ccccgcatcg aaacccgctg atcagccggt catcatcacc atcaccattg
     3241 agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     3301 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3361 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3421 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     3481 gggctctatg gcttctgagg cggaaagaac cagatcctct cttaaggtag catcgagatt
     3541 taaattaggg ataacagggt aatggcgcgg gccgcaggaa cccctagtga tggagttggc
     3601 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     3661 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     3721 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     3781 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3841 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3901 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3961 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     4021 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4081 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     4141 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4201 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     4261 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     4321 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     4381 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     4441 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4501 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4561 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4621 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4681 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4741 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4801 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4861 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4921 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4981 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5041 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5101 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5161 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5221 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5281 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5341 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5401 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5461 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5521 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5581 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5641 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5701 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5761 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     5821 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5881 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5941 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6001 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6061 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6121 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6181 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6241 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6301 tttgctcaca tgt
//
