LOCUS       Exported                6013 bp DNA     circular SYN 22-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 22, 2022 from SnapGene 6.0.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            613..1320
                     /label=mCherry
     gene            join(1345..2315,2317..2318,2320..2324,2326..2327,2329..2330,
                     2332..2347,2349..2350,2352..2353,2355,2358..2668)
                     /label=p62/SQSTM
     modified_base   2314..2331
                     /label=silent mutation siRNA5 BS
     variation       2347..2358
                     /label=DDDW335>338AAAA
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tcaaatggcg tcgctcaccg tgaaggccta ccttctgggc
     1381 aaggaggacg cggcgcgcga gattcgccgc ttcagcttct gctgcagccc cgagcctgag
     1441 gcggaagccg aggctgcggc gggtccggga ccctgcgagc ggctgctgag ccgggtggcc
     1501 gccctgttcc ccgcgctgcg gcctggcggc ttccaggcgc actaccgcga tgaggacggg
     1561 gacttggttg ccttttccag tgacgaggaa ttgacaatgg ccatgtccta cgtgaaggat
     1621 gacatcttcc gaatctacat taaagagaaa aaagagtgcc ggcgggacca ccgcccaccg
     1681 tgtgctcagg aggcgccccg caacatggtg caccccaatg tgatctgcga tggctgcaat
     1741 gggcctgtgg taggaacccg ctacaagtgc agcgtctgcc cagactacga cttgtgtagc
     1801 gtctgcgagg gaaagggctt gcaccggggg cacaccaagc tcgcattccc cagccccttc
     1861 gggcacctgt ctgagggctt ctcgcacagc cgctggctcc ggaaggtgaa acacggacac
     1921 ttcgggtggc caggatggga aatgggtcca ccaggaaact ggagcccacg tcctcctcgt
     1981 gcaggggagg cccgccctgg ccccacggca gaatcagctt ctggtccatc ggaggatccg
     2041 agtgtgaatt tcctgaagaa cgttggggag agtgtggcag ctgcccttag ccctctgggc
     2101 attgaagttg atatcgatgt ggagcacgga gggaaaagaa gccgcctgac ccccgtctct
     2161 ccagagagtt ccagcacaga ggagaagagc agctcacagc caagcagctg ctgctctgac
     2221 cccagcaagc cgggtgggaa tgttgagggc gccacgcagt ctctggcgga gcagatgagg
     2281 aagatcgcct tggagtccga ggggcgccct gaggagcaaa tggaatccga caactgttca
     2341 ggaggagctg ctgccgcgac ccatctgtct tcaaaagaag tggacccgtc tacaggtgaa
     2401 ctccagtccc tacagatgcc agaatccgaa gggccaagct ctctggaccc ctcccaggag
     2461 ggacccacag ggctgaagga agctgccttg tacccacatc tcccgccaga ggctgacccg
     2521 cggctgattg agtccctctc ccagatgctg tccatgggct tctctgatga aggcggctgg
     2581 ctcaccaggc tcctgcagac caagaactat gacatcggag cggctctgga caccatccag
     2641 tattcaaagc atcccccgcc gttgtgaccc gggatccacc ggatctagat aactgatcat
     2701 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     2761 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     2821 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2881 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg
     2941 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3001 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3061 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3121 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     3181 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     3241 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     3301 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3361 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3421 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3481 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     3541 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3601 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3661 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3721 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3781 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3841 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     3901 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     3961 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4021 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4081 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4141 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     4201 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     4261 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     4321 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     4381 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     4441 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     4501 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     4561 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     4621 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     4681 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     4741 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     4801 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     4861 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     4921 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     4981 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     5041 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     5101 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     5161 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5221 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5281 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5341 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5401 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5461 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5521 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5581 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5641 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5701 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5761 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5821 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5881 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5941 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6001 taccgccatg cat
//
