LOCUS       Exported                8066 bp ds-DNA     circular SYN 21-JUN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Tivan161
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 21, 2022 from SnapGene 5.1.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     769..789
                     /label=CMV Forward
                     /note="color: black; sequence: CGCAAATGGGCGGTAGGCGTG"
     primer_bind     863..882
                     /label=T7_F
                     /note="color: black; sequence: TAATACGACTCACTATAGGG"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     923..945
                     /label=Qtruncf
                     /note="color: black; sequence: gagctcggatccatgaggaggtg; 
                     added: 2021-06-30"
     primer_bind     complement(933..952)
                     /label=PCDNA Linear Reverse
                     /note="color: black; sequence: GCTGTTGCACCTCCTCATgg; added:
                     2021-06-30"
     CDS             935..1027
                     /codon_start=1
                     /label=QSOX1 SignalP
                     /note="color: #a6acb3"
                     /translation="MRRCNSGSGPPPSLLLLLLWLLAVPGANAAP"
     primer_bind     965..1011
                     /label=leu
                     /note="color: black; sequence: 
                     CCGCCGTCGCTGCTGCTGCTGCTGCTGTGGCTGCTCGCGGTTCCCGG; added: 
                     2021-06-30"
     primer_bind     1009..1027
                     /label=MegaP forward (qsox end of signal seq+pro)
                     /note="color: black; sequence: CGGCGCTAACGCGGCCCCG; added: 
                     2021-06-30"
     primer_bind     1009..1023
                     /label=MegaP forward (qsox end of signal seq)
                     /note="color: black; sequence: CGGCGCTAACGCGGC; added: 
                     2021-06-30"
     primer_bind     1019..1046
                     /label=OLI141_Muc2_H398A_F
                     /note="color: black; sequence: aggcggctccgccatcaccacc; 
                     added: 2020-04-06"
     CDS             1034..1051
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     primer_bind     1043..1093
                     /label=OLI135_Muc2D1_into N'term_His_TEV_F
                     /note="color: black; sequence: 
                     CACCATCACGAGAATCTCTATTTCCAGGGATCGGAGCTCCAGACAGAGGGC; added:
                     2020-03-10"
     misc_feature    1052..1072
                     /label=TEV site
                     /note="color: #00ff00
                     Cleavage site after base 1069"
     primer_bind     1052..1072
                     /label=OLI139_Tev site Forward
                     /note="color: black; sequence: GAGAATCTCTATTTCCAGGGA; 
                     added: 2020-03-26"
     CDS             1073..3586
                     /codon_start=1
                     /label=Muc2 - D1D2D'
                     /note="color: #993366"
                     /translation="SELQTEGRTRNHGHNVCSTWGNFHYKTFDGDVFRFPGLCDYNFAS
                     DCRGSYKEFAVHLKRGPGQAEAPAGVESILLTIKDDTIYLTRHLAVLNGAVVSTPHYSP
                     GLLIEKSDAYTKVYSRAGLTLMWNREDALMLELDTKFRNHTCGLCGDYNGLQSYSEFLS
                     DGVLFSPLEFGNMQKINQPDVVCEDPEEEVAPASCSEHRAECERLLTAEAFADCQDLVP
                     LEPYLRACQQDRCRCPGGDTCVCSTVAEFSRQCSHAGGRPGNWRTATLCPKTCPGNLVY
                     LESGSPCMDTCSHLEVSSLCEEHRMDGCFCPEGTVYDDIGDSGCVPVSQCHCRLHGHLY
                     TPGQEITNDCEQCVCNAGRWVCKDLPCPGTCALEGGSHITTFDGKTYTFHGDCYYVLAK
                     GDHNDSYALLGELAPCGSTDKQTCLKTVVLLADKKKNVVVFKSDGSVLLNELQVNLPHV
                     TASFSVFRPSSYHIMVSMAIGVRLQVQLAPVMQLFVTLDQASQGQVQGLCGNFNGLEGD
                     DFKTASGLVEATGAGFANTWKAQSSCHDKLDWLDDPCSLNIESANYAEHWCSLLKKTET
                     PFGRCHSAVDPAEYYKRCKYDTCNCQNNEDCLCAALSSYARACTAKGVMLWGWREHVCN
                     KDVGSCPNSQVFLYNLTTCQQTCRSLSEADSHCLEGFAPVDGCGCPDHTFLDEKGRCVP
                     LAKCSCYHRGLYLEAGDVVVRQEERCVCRDGRLHCRQIRLIGQSCTAPKIHMDCSNLTA
                     LATSKPRALSCQTLAAGYYHTECVSGCVCPDGLMDDGRGGCVVEKECPCVHNNDLYSSG
                     AKIKVDCNTCTCKRGRWVCTQAVCHG"
     primer_bind     1073..1101
                     /label=d1d2pre (begining of Muc2)
                     /note="color: black; sequence: 
                     tcggagctccagacagagggcagaacccg; added: 2021-06-30"
     primer_bind     complement(1187..1207)
                     /label=Muc2-C59Rev
                     /note="color: black; sequence: GGAGGCGAAGTTGTAGTCGCA; 
                     added: 2021-06-30"
     primer_bind     1463..1495
                     /label=OLI322 - Muc2-E156A_F
                     /note="color: black; sequence: 
                     gatgcactcatgctggcgctggacactaagttc; added: 2021-06-30"
     primer_bind     complement(1961..2014)
                     /label=D1-Cu-mut
                     /note="color: black; sequence: 
                     GCCTTCTGGGCAGAAACAGCCGTCataGCGcTcCTCCTCGCACAGGCTGCTCAC; 
                     added: 2021-06-30"
     primer_bind     complement(2045..2065)
                     /label=D1TIL-trunc
                     /note="color: black; sequence: 
                     GAATTCTCAATGATGGTGATGATGGTGGCCGCCGCACTGGCTCACAGGAACGCA; 
                     added: 2021-06-30"
     primer_bind     complement(2045..2065)
                     /label=His-TEV-D1 short
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCAggcGCACTGGCTCACAGGAACGCA; 
                     added: 2021-06-30"
     primer_bind     2147..2196
                     /label=OLI408 - D2 into His-TEV vec_F
                     /note="color: black; sequence: 
                     CGCTGGGTGTGCAAAGACCTGCCCTGCCCCGGCACCTGTGCCCTGGAAGG; added: 
                     2021-06-17"
     primer_bind     complement(2155..2175)
                     /label=MUC5B_D1_clon_R
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCAagggcatggcaggtctttgcac; 
                     added: 2021-06-30"
     primer_bind     complement(2156..2176)
                     /label=OLI136 - Muc2D1_into N'term_His_TEV_R
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCAGGGGCAGGGCAGGTCTTTGCA; added:
                     2020-03-10"
     primer_bind     complement(2159..2179)
                     /label=Muc2_D1_Trypsin_N'
                     /note="color: black; sequence: 
                     GCAGAATTCTCAATGATGGTGATGATGGTGGCCGCGCAGGCCGGGGCAGGGCAGGTCTT
                     T; added: 2021-06-30"
     primer_bind     complement(2159..2179)
                     /label=trunc_Muc2_D1
                     /note="color: black; sequence: 
                     GCAGAATTCTCAATGATGGTGATGATGGTGGCCGGGGCAGGGCAGGTCTTT; added:
                     2021-06-30"
     primer_bind     2194..2215
                     /label=OLI141_Muc2_H398A_F
                     /note="color: black; sequence: aggcggctccgccatcaccacc; 
                     added: 2020-04-06"
     primer_bind     2313..2333
                     /label=OLI104-gap
                     /note="color: black; sequence: CCTGTGGCTCCACAGACAAGC; 
                     added: 2020-04-06"
     primer_bind     complement(2473..2493)
                     /label=OLI247-Muc2_V494_Rev
                     /note="color: black; sequence: ACCATGATGTGGTAGGAAGAC; 
                     added: 2020-04-23"
     primer_bind     complement(2568..2599)
                     /label=OLI408 - D2 into His-TEV vec_F
                     /note="color: black; sequence: 
                     CGCTGGGTGTGCAAAGACCTGCCCTGCCCCGGCACCTGTGCCCTGGAAGG; added: 
                     2021-06-17"
     primer_bind     2800..2834
                     /label=Muc2 D2 His to Ala
                     /note="color: black; sequence: 
                     CCCCTTTGGCAGGTGCGCCTCGGCTGTGGACCCTG; added: 2021-06-30"
     primer_bind     2800..2834
                     /label=Muc2 D2 His to Gln
                     /note="color: black; sequence: 
                     CCCCTTTGGCAGGTGCCAGTCGGCTGTGGACCCTG; added: 2021-06-30"
     primer_bind     3077..3098
                     /label=OLI269-nils1
                     /note="color: black; sequence: ctcgagggctttgcgcctgtgg; 
                     added: 2020-05-05"
     primer_bind     complement(3471..3493)
                     /label=OLI143_Muc2_H824A_R
                     /note="color: black; sequence: GTCGTTGTTAgcGACGCAAGGGC; 
                     added: 2020-04-06"
     primer_bind     3510..3530
                     /label=OLI100-Muc2-Ala833Fwd
                     /note="color: black; sequence: CCAAGATCAAGGTGGACTGCA; 
                     added: 2020-04-06"
     primer_bind     complement(3567..3616)
                     /label=OLI409 - D2 into His-TEV vec_R
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCAGCCATGGCACACAGCCTGGG; added: 
                     2021-06-17"
     primer_bind     complement(3643..3695)
                     /label=shRNA (GIPZ) region into pcDNA3 - R
                     /note="color: black; sequence: 
                     caacagatggctggcaactagaaggcacagtcgaggctgatcagcgggtttaa; 
                     added: 2021-06-30"
     primer_bind     complement(3660..3682)
                     /label=OLI27_BGH_R_72C
                     /note="color: black; sequence: gcaactagaaggcacagtcgagg"
     primer_bind     complement(3660..3677)
                     /label=BGH rev
                     /note="color: black; sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    3666..3777
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     3701..3732
                     /label=OLI408 - D2 into His-TEV vec_F
                     /note="color: black; sequence: 
                     CGCTGGGTGTGCAAAGACCTGCCCTGCCCCGGCACCTGTGCCCTGGAAGG; added: 
                     2021-06-17"
     rep_origin      3936..4364
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4378..4707
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      4558..4693
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     complement(4620..4639)
                     /label=sv40-rev
                     /note="color: black; sequence: CCTCGGCCTCTGCATAAATA"
     CDS             4774..5568
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5742..5863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(5779..5798)
                     /label=SV40 pa-R
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     complement(5912..5928)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(5926..5945)
                     /label=pQE30_F
                     /note="color: black; sequence: CGGATAACAATTTCACACAG; added:
                     2021-06-30"
     protein_bind    5936..5952
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(5960..5990)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 5960 .. 5966 / #ffffff / -10
                        2: 5967 .. 5984 / #ffffff
                        3: 5985 .. 5990 / #ffffff / -35"
     protein_bind    6005..6026
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(6314..6899)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(7070..7930)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7070 .. 7861 / #ccffcc
                        2: 7862 .. 7930 / #ccffcc / signal sequence
                     Cleavage site after base 7861"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7931..8035)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccatgagg aggtgcaaca gcggctccgg
      961 gccgccgccg tcgctgctgc tgctgttgct gtggctgctc gcggttcccg gcgctaacgc
     1021 ggccccgcag ggtcatcatc atcaccatca cgagaatctc tatttccagg gatcggagct
     1081 ccagacagag ggcagaaccc gaaaccacgg ccacaacgtc tgcagcacct ggggcaactt
     1141 ccactacaag accttcgacg gggacgtctt ccgcttcccc ggcctctgcg actacaactt
     1201 cgcctccgac tgccgaggct cctacaagga atttgctgtg cacctgaagc ggggtccggg
     1261 ccaggctgag gcccccgccg gggtggagtc catcctgctg accatcaagg atgacaccat
     1321 ctacctcacc cgccacctgg ctgtgcttaa cggggccgtg gtcagcaccc cgcactacag
     1381 ccccgggctg ctcattgaga agagcgatgc ctacaccaaa gtctactccc gcgccggcct
     1441 caccctcatg tggaaccggg aggatgcact catgctggag ctggacacta agttccggaa
     1501 ccacacctgt ggcctctgcg gggactacaa cggcctgcag agctattcag aattcctctc
     1561 tgacggcgtg ctcttcagtc ccctggagtt tgggaacatg cagaagatca accaacccga
     1621 tgtggtgtgt gaggatcccg aggaggaggt ggcccccgca tcctgctccg agcaccgcgc
     1681 cgagtgtgag aggctgctga ccgccgaggc cttcgcggac tgtcaggacc tggtgccgct
     1741 ggagccgtat ctgcgcgcct gccagcagga ccgctgccgg tgcccgggcg gtgacacctg
     1801 cgtctgcagc accgtggccg agttctcccg ccagtgctcc cacgccggcg gccggcccgg
     1861 gaactggagg accgccacgc tctgccccaa gacctgcccc gggaacctgg tgtacctgga
     1921 gagcggctcg ccctgcatgg acacctgctc acacctggag gtgagcagcc tgtgcgagga
     1981 gcaccgcatg gacggctgtt tctgcccaga aggcaccgta tatgacgaca tcggggacag
     2041 tggctgcgtt cctgtgagcc agtgccactg caggctgcac ggacacctgt acacaccggg
     2101 ccaggagatc accaatgact gcgagcagtg tgtctgtaac gctggccgct gggtgtgcaa
     2161 agacctgccc tgccccggca cctgtgccct ggaaggcggc tcccacatca ccaccttcga
     2221 tgggaagacg tacaccttcc acggggactg ctactatgtc ctggccaagg gtgaccacaa
     2281 cgattcctat gctctcctgg gcgagctggc cccctgtggc tccacagaca agcagacctg
     2341 cctgaagacg gtggtgctgc tggctgacaa gaagaagaat gtggtggtct tcaagtccga
     2401 tggcagtgta ctgctcaacg agctgcaggt gaacctgccc cacgtgaccg cgagcttctc
     2461 tgtcttccgc ccgtcttcct accacatcat ggtgagcatg gccattggcg tccggctgca
     2521 ggtgcagctg gccccagtca tgcaactctt tgtgacactg gaccaggcct cccaggggca
     2581 ggtgcagggc ctctgcggga acttcaacgg cctggaaggt gacgacttca agacggccag
     2641 cgggctggtg gaggccacgg gggccggctt tgccaacacc tggaaggcac agtcaagctg
     2701 ccatgacaag ctggactggt tggacgatcc ctgctccctg aacatcgaga gcgccaacta
     2761 cgccgagcac tggtgctccc tcctgaagaa gacagagacc ccctttggca ggtgccactc
     2821 ggctgtggac cctgctgagt attacaagag gtgcaaatat gacacgtgta actgtcagaa
     2881 caatgaggac tgcctgtgcg ccgccctgtc ctcctacgcg cgcgcctgca ccgccaaggg
     2941 cgtcatgctg tggggctggc gggagcatgt ctgcaacaag gatgtgggct cctgccccaa
     3001 ctcgcaggtc ttcctgtaca acctgaccac ctgccagcag acctgccgct ccctctccga
     3061 ggccgacagc cactgtctcg agggctttgc gcctgtggac ggctgcggct gccctgacca
     3121 caccttcctg gacgagaagg gccgctgcgt acccctggcc aagtgctcct gttaccaccg
     3181 cggtctctac ctggaggcgg gggatgtggt cgtcaggcag gaagaacgat gtgtgtgccg
     3241 ggatgggagg ctgcactgta ggcagatccg gctgatcggc cagagctgca cggccccaaa
     3301 gatccacatg gactgcagca acctgactgc actggccacc tcgaagcccc gagccctcag
     3361 ctgccagacg ctggccgccg gctattacca cacagagtgt gtcagtggct gtgtgtgccc
     3421 cgacgggctg atggatgacg gccggggtgg ctgtgtggtg gagaaggaat gcccttgcgt
     3481 ccataacaac gacctgtatt cttccggcgc caagatcaag gtggactgca atacctgcac
     3541 ctgcaagaga ggacgctggg tgtgcaccca ggctgtgtgc catggctgag aattctgcag
     3601 atatccagca cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc
     3661 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     3721 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     3781 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     3841 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     3901 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     3961 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4021 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4081 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4141 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4201 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4261 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4321 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     4381 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4441 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4501 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4561 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4621 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     4681 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     4741 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4801 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4861 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4921 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     4981 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5041 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5101 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5161 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5221 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5281 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5341 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5401 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5461 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5521 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5581 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5641 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5701 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     5761 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5821 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     5881 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5941 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6001 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6061 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6121 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6181 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6241 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6301 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6361 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6421 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6481 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6541 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6601 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6661 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6721 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6781 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6841 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6901 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6961 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7021 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7081 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7141 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7201 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7261 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7321 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7381 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7441 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7501 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7561 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7621 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7681 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7741 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7801 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7861 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7921 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7981 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8041 ttccccgaaa agtgccacct gacgtc
//
