LOCUS       Exported                9269 bp ds-DNA     circular SYN 21-JUN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Tivan161
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 21, 2022 from SnapGene 5.1.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3587..4789
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     769..789
                     /label=CMV Forward
                     /note="color: black; sequence: CGCAAATGGGCGGTAGGCGTG"
     primer_bind     863..882
                     /label=T7_F
                     /note="color: black; sequence: TAATACGACTCACTATAGGG"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     923..945
                     /label=Qtruncf
                     /note="color: black; sequence: gagctcggatccatgaggaggtg; 
                     added: 2021-06-30"
     primer_bind     933..955
                     /label=OLI65
                     /note="color: black; sequence: 
                     ttccatttcaggtgtcgtgagggatccaccATGAGGAGGTGCAACAGCGGC; added:
                     2021-08-08"
     primer_bind     complement(933..952)
                     /label=PCDNA Linear Reverse
                     /note="color: black; sequence: GCTGTTGCACCTCCTCATgg; added:
                     2021-06-30"
     primer_bind     934..953
                     /label=OLI90
                     /note="color: black; sequence: 
                     ACCGACTCTAGAGGATCCACTAGTCATGAGGAGGTGCAACAGCG; added: 
                     2021-08-08"
     CDS             935..1027
                     /codon_start=1
                     /label=QSOX1 SignalP
                     /note="color: #a6acb3"
                     /translation="MRRCNSGSGPPPSLLLLLLWLLAVPGANAAP"
     primer_bind     965..1011
                     /label=leu
                     /note="color: black; sequence: 
                     CCGCCGTCGCTGCTGCTGCTGCTGCTGTGGCTGCTCGCGGTTCCCGG; added: 
                     2021-06-30"
     primer_bind     1008..1027
                     /label=OLI271
                     /note="color: black; sequence: 
                     ccggcgctaacgcggccccgtcggagctccagacagagggc; added: 
                     2021-08-08"
     primer_bind     1009..1027
                     /label=MegaP forward (qsox end of signal seq+pro)
                     /note="color: black; sequence: CGGCGCTAACGCGGCCCCG; added: 
                     2021-06-30"
     primer_bind     1009..1023
                     /label=MegaP forward (qsox end of signal seq)
                     /note="color: black; sequence: CGGCGCTAACGCGGC; added: 
                     2021-06-30"
     primer_bind     complement(1013..1027)
                     /label=OLI383
                     /note="color: black; sequence: CGGGGCCGCGTTAGC; added: 
                     2021-08-08"
     primer_bind     1019..1046
                     /label=OLI141_Muc2_H398A_F
                     /note="color: black; sequence: aggcggctccgccatcaccacc; 
                     added: 2020-04-06"
     CDS             1034..1051
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     primer_bind     1043..1093
                     /label=OLI135_Muc2D1_into N'term_His_TEV_F
                     /note="color: black; sequence: 
                     CACCATCACGAGAATCTCTATTTCCAGGGATCGGAGCTCCAGACAGAGGGC; added:
                     2020-03-10"
     primer_bind     1043..1072
                     /label=OLI426
                     /note="color: black; sequence: 
                     CACCATCACGAGAATCTCTATTTCCAGGGAACCTGCTCCATTTACGGGAGTG; 
                     added: 2021-08-08"
     primer_bind     1043..1072
                     /label=OLI425
                     /note="color: black; sequence: 
                     CACCATCACGAGAATCTCTATTTCCAGGGAGGCACCTGTGCCCTGGAAGG; added: 
                     2021-08-08"
     misc_feature    1052..1072
                     /label=TEV site
                     /note="color: #00ff00
                     Cleavage site after base 1069"
     primer_bind     1052..1072
                     /label=OLI139_Tev site Forward
                     /note="color: black; sequence: GAGAATCTCTATTTCCAGGGA; 
                     added: 2020-03-26"
     CDS             1073..4789
                     /codon_start=1
                     /label=Muc2 - D1D2D'D3
                     /note="color: #993366"
                     /translation="SELQTEGRTRNHGHNVCSTWGNFHYKTFDGDVFRFPGLCDYNFAS
                     DCRGSYKEFAVHLKRGPGQAEAPAGVESILLTIKDDTIYLTRHLAVLNGAVVSTPHYSP
                     GLLIEKSDAYTKVYSRAGLTLMWNREDALMLELDTKFRNHTCGLCGDYNGLQSYSEFLS
                     DGVLFSPLEFGNMQKINQPDVVCEDPEEEVAPASCSEHRAECERLLTAEAFADCQDLVP
                     LEPYLRACQQDRCRCPGGDTCVCSTVAEFSRQCSHAGGRPGNWRTATLCPKTCPGNLVY
                     LESGSPCMDTCSHLEVSSLCEEHRMDGCFCPEGTVYDDIGDSGCVPVSQCHCRLHGHLY
                     TPGQEITNDCEQCVCNAGRWVCKDLPCPGTCALEGGSHITTFDGKTYTFHGDCYYVLAK
                     GDHNDSYALLGELAPCGSTDKQTCLKTVVLLADKKKNVVVFKSDGSVLLNELQVNLPHV
                     TASFSVFRPSSYHIMVSMAIGVRLQVQLAPVMQLFVTLDQASQGQVQGLCGNFNGLEGD
                     DFKTASGLVEATGAGFANTWKAQSSCHDKLDWLDDPCSLNIESANYAEHWCSLLKKTET
                     PFGRCHSAVDPAEYYKRCKYDTCNCQNNEDCLCAALSSYARACTAKGVMLWGWREHVCN
                     KDVGSCPNSQVFLYNLTTCQQTCRSLSEADSHCLEGFAPVDGCGCPDHTFLDEKGRCVP
                     LAKCSCYHRGLYLEAGDVVVRQEERCVCRDGRLHCRQIRLIGQSCTAPKIHMDCSNLTA
                     LATSKPRALSCQTLAAGYYHTECVSGCVCPDGLMDDGRGGCVVEKECPCVHNNDLYSSG
                     AKIKVDCNTCTCKRGRWVCTQAVCHGTCSIYGSGHYITFDGKYYDFDGHCSYVAVQDYC
                     GQNSSLGSFSIITENVPCGTTGVTCSKAIKIFMGRTELKLEDKHRVVIQRDEGHHVAYT
                     TREVGQYLVVESSTGIIVIWDKRTTVFIKLAPSYKGTVCGLCGNFDHRSNNDFTTRDHM
                     VVSSELDFGNSWKEAPTCPDVSTNPEPCSLNPHRRSWAEKQCSILKSSVFSICHSKVDP
                     KPFYEACVHDSCSCDTGGDCECFCSAVASYAQECTKEGACVFWRTPDLCPIFCDYYNPP
                     HECEWHYEPCGNRSFETCRTINGIHSNISVSYLEGCYPRCPKDRPIYEEDLKKCVTADK
                     CGCYVEDTHYPPGASVPTEETCKSCVCTNSSQVVCRPEEGKILNQTQDGAFCYWEICGP
                     NGTVEKHFNICSIT"
     primer_bind     1073..1101
                     /label=d1d2pre (begining of Muc2)
                     /note="color: black; sequence: 
                     tcggagctccagacagagggcagaacccg; added: 2021-06-30"
     primer_bind     1073..1093
                     /label=OLI271
                     /note="color: black; sequence: 
                     ccggcgctaacgcggccccgtcggagctccagacagagggc; added: 
                     2021-08-08"
     primer_bind     complement(1187..1207)
                     /label=Muc2-C59Rev
                     /note="color: black; sequence: GGAGGCGAAGTTGTAGTCGCA; 
                     added: 2021-06-30"
     primer_bind     1463..1495
                     /label=OLI322 - Muc2-E156A_F
                     /note="color: black; sequence: 
                     gatgcactcatgctggcgctggacactaagttc; added: 2021-06-30"
     primer_bind     1744..1787
                     /label=leu
                     /note="color: black; sequence: 
                     CCGCCGTCGCTGCTGCTGCTGCTGCTGTGGCTGCTCGCGGTTCCCGG; added: 
                     2021-06-30"
     primer_bind     complement(1961..2014)
                     /label=D1-Cu-mut
                     /note="color: black; sequence: 
                     GCCTTCTGGGCAGAAACAGCCGTCataGCGcTcCTCCTCGCACAGGCTGCTCAC; 
                     added: 2021-06-30"
     primer_bind     complement(1965..2007)
                     /label=OLI387
                     /note="color: black; sequence: 
                     gggcagaaacagccgtcatagcgctcctcctcgcacaggctgc; added: 
                     2021-08-08"
     primer_bind     complement(2045..2065)
                     /label=D1TIL-trunc
                     /note="color: black; sequence: 
                     GAATTCTCAATGATGGTGATGATGGTGGCCGCCGCACTGGCTCACAGGAACGCA; 
                     added: 2021-06-30"
     primer_bind     complement(2045..2065)
                     /label=His-TEV-D1 short
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCAggcGCACTGGCTCACAGGAACGCA; 
                     added: 2021-06-30"
     primer_bind     2147..2196
                     /label=OLI408 - D2 into His-TEV vec_F
                     /note="color: black; sequence: 
                     CGCTGGGTGTGCAAAGACCTGCCCTGCCCCGGCACCTGTGCCCTGGAAGG; added: 
                     2021-06-17"
     primer_bind     complement(2155..2175)
                     /label=MUC5B_D1_clon_R
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCAagggcatggcaggtctttgcac; 
                     added: 2021-06-30"
     primer_bind     complement(2155..2175)
                     /label=OLI385
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCAGTGATGGTGATGATGATGTCCCTGGAAAT
                     AGAGATTCTCagggcatggcaggtctttgcac; added: 2021-08-08"
     primer_bind     complement(2156..2176)
                     /label=OLI136 - Muc2D1_into N'term_His_TEV_R
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCAGGGGCAGGGCAGGTCTTTGCA; added:
                     2020-03-10"
     primer_bind     complement(2158..2179)
                     /label=OLI379
                     /note="color: black; sequence: 
                     gtgatgatggtggccgaagcagggcaggtctttg; added: 2021-08-08"
     primer_bind     complement(2159..2213)
                     /label=Muc2_D1_Trypsin_N'
                     /note="color: black; sequence: 
                     GCAGAATTCTCAATGATGGTGATGATGGTGGCCGCGCAGGCCGGGGCAGGGCAGGTCTT
                     T; added: 2021-06-30"
     primer_bind     complement(2159..2179)
                     /label=trunc_Muc2_D1
                     /note="color: black; sequence: 
                     GCAGAATTCTCAATGATGGTGATGATGGTGGCCGGGGCAGGGCAGGTCTTT; added:
                     2021-06-30"
     primer_bind     2177..2196
                     /label=OLI425
                     /note="color: black; sequence: 
                     CACCATCACGAGAATCTCTATTTCCAGGGAGGCACCTGTGCCCTGGAAGG; added: 
                     2021-08-08"
     primer_bind     2194..2215
                     /label=OLI141_Muc2_H398A_F
                     /note="color: black; sequence: aggcggctccgccatcaccacc; 
                     added: 2020-04-06"
     primer_bind     2313..2333
                     /label=OLI104-gap
                     /note="color: black; sequence: CCTGTGGCTCCACAGACAAGC; 
                     added: 2020-04-06"
     primer_bind     complement(2473..2493)
                     /label=OLI247-Muc2_V494_Rev
                     /note="color: black; sequence: ACCATGATGTGGTAGGAAGAC; 
                     added: 2020-04-23"
     primer_bind     complement(2568..2599)
                     /label=OLI408 - D2 into His-TEV vec_F
                     /note="color: black; sequence: 
                     CGCTGGGTGTGCAAAGACCTGCCCTGCCCCGGCACCTGTGCCCTGGAAGG; added: 
                     2021-06-17"
     primer_bind     complement(2568..2585)
                     /label=OLI425
                     /note="color: black; sequence: 
                     CACCATCACGAGAATCTCTATTTCCAGGGAGGCACCTGTGCCCTGGAAGG; added: 
                     2021-08-08"
     primer_bind     2800..2834
                     /label=Muc2 D2 His to Ala
                     /note="color: black; sequence: 
                     CCCCTTTGGCAGGTGCGCCTCGGCTGTGGACCCTG; added: 2021-06-30"
     primer_bind     2800..2834
                     /label=Muc2 D2 His to Gln
                     /note="color: black; sequence: 
                     CCCCTTTGGCAGGTGCCAGTCGGCTGTGGACCCTG; added: 2021-06-30"
     primer_bind     2806..2827
                     /label=OLI142-Muc2_H602A_F
                     /note="color: black; sequence: tggcaggtgcgcctcggctgtg; 
                     added: 2020-04-19"
     primer_bind     3077..3098
                     /label=OLI269-nils1
                     /note="color: black; sequence: ctcgagggctttgcgcctgtgg; 
                     added: 2020-05-05"
     primer_bind     complement(3471..3493)
                     /label=OLI143_Muc2_H824A_R
                     /note="color: black; sequence: GTCGTTGTTAgcGACGCAAGGGC; 
                     added: 2020-04-06"
     primer_bind     3510..3530
                     /label=OLI100-Muc2-Ala833Fwd
                     /note="color: black; sequence: CCAAGATCAAGGTGGACTGCA; 
                     added: 2020-04-06"
     primer_bind     3557..3586
                     /label=OLI428
                     /note="color: black; sequence: 
                     tgggtgtgcacccaggctgtgtgccatggc; added: 2021-08-08"
     primer_bind     complement(3567..3586)
                     /label=OLI409 - D2 into His-TEV vec_R
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCAGCCATGGCACACAGCCTGGG; added: 
                     2021-06-17"
     primer_bind     complement(3579..3588)
                     /label=OLI428
                     /note="color: black; sequence: 
                     tgggtgtgcacccaggctgtgtgccatggc; added: 2021-08-08"
     primer_bind     3580..3608
                     /label=OLI426
                     /note="color: black; sequence: 
                     CACCATCACGAGAATCTCTATTTCCAGGGAACCTGCTCCATTTACGGGAGTG; 
                     added: 2021-08-08"
     primer_bind     complement(3584..3610)
                     /label=OLI272
                     /note="color: black; sequence: gccactcccgtaaatggagcaggtgcc;
                     added: 2021-08-08"
     primer_bind     complement(3701..3720)
                     /label=OLI218
                     /note="color: black; sequence: ATGATGCTGAATGAGCCCAG; added:
                     2021-08-08"
     primer_bind     3952..3974
                     /label=OLI266
                     /note="color: black; sequence: GGCTCCCTCCTACAAGGGCACCG; 
                     added: 2021-08-08"
     primer_bind     4182..4216
                     /label=OLI402
                     /note="color: black; sequence: 
                     GCGTGTTCAGCATCTGcGCCAGCAAGGTGGACCCC; added: 2021-08-08"
     primer_bind     4182..4216
                     /label=OLI403
                     /note="color: black; sequence: 
                     GCGTGTTCAGCATCTGCCAGAGCAAGGTGGACCCC; added: 2021-08-08"
     primer_bind     4563..4585
                     /label=OLI265
                     /note="color: black; sequence: CAGACAAGTGTGGCTGCTATGTC; 
                     added: 2021-08-08"
     primer_bind     complement(4765..4819)
                     /label=OLI429
                     /note="color: black; sequence: 
                     ccactgtgctggatatctgcagaattctcacgtaatggaacagatgttgaagtgc; 
                     added: 2021-08-08"
     primer_bind     complement(4765..4789)
                     /label=OLI430
                     /note="color: black; sequence: cgtaatggaacagatgttgaagtgc; 
                     added: 2021-08-08"
     primer_bind     complement(4766..4819)
                     /label=OLI427
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCACGTAATGGAACAGATGTTGAAGTG; 
                     added: 2021-08-08"
     primer_bind     complement(4790..4819)
                     /label=OLI136 - Muc2D1_into N'term_His_TEV_R
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCAGGGGCAGGGCAGGTCTTTGCA; added:
                     2020-03-10"
     primer_bind     complement(4790..4819)
                     /label=OLI409 - D2 into His-TEV vec_R
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCAGCCATGGCACACAGCCTGGG; added: 
                     2021-06-17"
     primer_bind     complement(4790..4819)
                     /label=His-TEV-D1 short
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCAggcGCACTGGCTCACAGGAACGCA; 
                     added: 2021-06-30"
     primer_bind     complement(4790..4819)
                     /label=MUC5B_D1_clon_R
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCAagggcatggcaggtctttgcac; 
                     added: 2021-06-30"
     primer_bind     complement(4790..4819)
                     /label=OLI385
                     /note="color: black; sequence: 
                     CCACTGTGCTGGATATCTGCAGAATTCTCAGTGATGGTGATGATGATGTCCCTGGAAAT
                     AGAGATTCTCagggcatggcaggtctttgcac; added: 2021-08-08"
     primer_bind     complement(4846..4898)
                     /label=shRNA (GIPZ) region into pcDNA3 - R
                     /note="color: black; sequence: 
                     caacagatggctggcaactagaaggcacagtcgaggctgatcagcgggtttaa; 
                     added: 2021-06-30"
     primer_bind     4862..4873
                     /label=OLI142-Muc2_H602A_F
                     /note="color: black; sequence: tggcaggtgcgcctcggctgtg; 
                     added: 2020-04-19"
     primer_bind     complement(4863..4885)
                     /label=OLI27_BGH_R_72C
                     /note="color: black; sequence: gcaactagaaggcacagtcgagg"
     primer_bind     complement(4863..4880)
                     /label=BGH rev
                     /note="color: black; sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    4869..4980
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     4904..4935
                     /label=OLI408 - D2 into His-TEV vec_F
                     /note="color: black; sequence: 
                     CGCTGGGTGTGCAAAGACCTGCCCTGCCCCGGCACCTGTGCCCTGGAAGG; added: 
                     2021-06-17"
     primer_bind     4926..4935
                     /label=OLI425
                     /note="color: black; sequence: 
                     CACCATCACGAGAATCTCTATTTCCAGGGAGGCACCTGTGCCCTGGAAGG; added: 
                     2021-08-08"
     rep_origin      5139..5567
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5581..5910
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5761..5896
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     complement(5823..5842)
                     /label=sv40-rev
                     /note="color: black; sequence: CCTCGGCCTCTGCATAAATA"
     CDS             5977..6771
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6945..7066
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6982..7001)
                     /label=SV40 pa-R
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     complement(7115..7131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(7129..7148)
                     /label=pQE30_F
                     /note="color: black; sequence: CGGATAACAATTTCACACAG; added:
                     2021-06-30"
     protein_bind    7139..7155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(7163..7193)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 7163 .. 7169 / #ffffff / -10
                        2: 7170 .. 7187 / #ffffff
                        3: 7188 .. 7193 / #ffffff / -35"
     protein_bind    7208..7229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(7517..8102)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(8273..9133)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8273 .. 9064 / #ccffcc
                        2: 9065 .. 9133 / #ccffcc / signal sequence
                     Cleavage site after base 9064"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9134..9238)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccatgagg aggtgcaaca gcggctccgg
      961 gccgccgccg tcgctgctgc tgctgttgct gtggctgctc gcggttcccg gcgctaacgc
     1021 ggccccgcag ggtcatcatc atcaccatca cgagaatctc tatttccagg gatcggagct
     1081 ccagacagag ggcagaaccc gaaaccacgg ccacaacgtc tgcagcacct ggggcaactt
     1141 ccactacaag accttcgacg gggacgtctt ccgcttcccc ggcctctgcg actacaactt
     1201 cgcctccgac tgccgaggct cctacaagga atttgctgtg cacctgaagc ggggtccggg
     1261 ccaggctgag gcccccgccg gggtggagtc catcctgctg accatcaagg atgacaccat
     1321 ctacctcacc cgccacctgg ctgtgcttaa cggggccgtg gtcagcaccc cgcactacag
     1381 ccccgggctg ctcattgaga agagcgatgc ctacaccaaa gtctactccc gcgccggcct
     1441 caccctcatg tggaaccggg aggatgcact catgctggag ctggacacta agttccggaa
     1501 ccacacctgt ggcctctgcg gggactacaa cggcctgcag agctattcag aattcctctc
     1561 tgacggcgtg ctcttcagtc ccctggagtt tgggaacatg cagaagatca accaacccga
     1621 tgtggtgtgt gaggatcccg aggaggaggt ggcccccgca tcctgctccg agcaccgcgc
     1681 cgagtgtgag aggctgctga ccgccgaggc cttcgcggac tgtcaggacc tggtgccgct
     1741 ggagccgtat ctgcgcgcct gccagcagga ccgctgccgg tgcccgggcg gtgacacctg
     1801 cgtctgcagc accgtggccg agttctcccg ccagtgctcc cacgccggcg gccggcccgg
     1861 gaactggagg accgccacgc tctgccccaa gacctgcccc gggaacctgg tgtacctgga
     1921 gagcggctcg ccctgcatgg acacctgctc acacctggag gtgagcagcc tgtgcgagga
     1981 gcaccgcatg gacggctgtt tctgcccaga aggcaccgta tatgacgaca tcggggacag
     2041 tggctgcgtt cctgtgagcc agtgccactg caggctgcac ggacacctgt acacaccggg
     2101 ccaggagatc accaatgact gcgagcagtg tgtctgtaac gctggccgct gggtgtgcaa
     2161 agacctgccc tgccccggca cctgtgccct ggaaggcggc tcccacatca ccaccttcga
     2221 tgggaagacg tacaccttcc acggggactg ctactatgtc ctggccaagg gtgaccacaa
     2281 cgattcctat gctctcctgg gcgagctggc cccctgtggc tccacagaca agcagacctg
     2341 cctgaagacg gtggtgctgc tggctgacaa gaagaagaat gtggtggtct tcaagtccga
     2401 tggcagtgta ctgctcaacg agctgcaggt gaacctgccc cacgtgaccg cgagcttctc
     2461 tgtcttccgc ccgtcttcct accacatcat ggtgagcatg gccattggcg tccggctgca
     2521 ggtgcagctg gccccagtca tgcaactctt tgtgacactg gaccaggcct cccaggggca
     2581 ggtgcagggc ctctgcggga acttcaacgg cctggaaggt gacgacttca agacggccag
     2641 cgggctggtg gaggccacgg gggccggctt tgccaacacc tggaaggcac agtcaagctg
     2701 ccatgacaag ctggactggt tggacgatcc ctgctccctg aacatcgaga gcgccaacta
     2761 cgccgagcac tggtgctccc tcctgaagaa gacagagacc ccctttggca ggtgccactc
     2821 ggctgtggac cctgctgagt attacaagag gtgcaaatat gacacgtgta actgtcagaa
     2881 caatgaggac tgcctgtgcg ccgccctgtc ctcctacgcg cgcgcctgca ccgccaaggg
     2941 cgtcatgctg tggggctggc gggagcatgt ctgcaacaag gatgtgggct cctgccccaa
     3001 ctcgcaggtc ttcctgtaca acctgaccac ctgccagcag acctgccgct ccctctccga
     3061 ggccgacagc cactgtctcg agggctttgc gcctgtggac ggctgcggct gccctgacca
     3121 caccttcctg gacgagaagg gccgctgcgt acccctggcc aagtgctcct gttaccaccg
     3181 cggtctctac ctggaggcgg gggatgtggt cgtcaggcag gaagaacgat gtgtgtgccg
     3241 ggatgggagg ctgcactgta ggcagatccg gctgatcggc cagagctgca cggccccaaa
     3301 gatccacatg gactgcagca acctgactgc actggccacc tcgaagcccc gagccctcag
     3361 ctgccagacg ctggccgccg gctattacca cacagagtgt gtcagtggct gtgtgtgccc
     3421 cgacgggctg atggatgacg gccggggtgg ctgtgtggtg gagaaggaat gcccttgcgt
     3481 ccataacaac gacctgtatt cttccggcgc caagatcaag gtggactgca atacctgcac
     3541 ctgcaagaga ggacgctggg tgtgcaccca ggctgtgtgc catggcacct gctccattta
     3601 cgggagtggc cactacatca cctttgatgg gaagtactac gactttgacg gacactgctc
     3661 ctacgtggct gttcaggact actgcggcca gaactcctca ctgggctcat tcagcatcat
     3721 caccgagaac gtcccctgtg gcactacggg cgtcacctgc tccaaggcca ttaagatctt
     3781 catggggagg acggagctga agttggaaga caagcaccgt gtggtgatcc agcgtgatga
     3841 gggtcaccac gtggcctaca ccacgcggga ggtgggccag tacctggtgg tggagtccag
     3901 cacgggcatc atcgtcatct gggacaagag gaccaccgtg ttcatcaagc tggctccctc
     3961 ctacaagggc accgtgtgtg gcctgtgtgg gaactttgac caccgctcca acaacgactt
     4021 caccacgcgg gaccacatgg tggtgagcag cgagctggac ttcgggaaca gctggaagga
     4081 ggcccccacc tgcccagatg tgagcaccaa ccccgagccc tgcagcctga acccgcaccg
     4141 ccgctcctgg gccgagaagc agtgcagcat cctcaaaagc agcgtgttca gcatctgcca
     4201 cagcaaggtg gaccccaagc ccttctacga ggcctgtgtg cacgactcgt gctcctgtga
     4261 cacgggtggg gactgtgagt gcttctgctc tgccgtggcc tcctacgccc aggagtgtac
     4321 caaagagggg gcctgcgtgt tctggaggac gccggacctg tgccccatat tctgcgacta
     4381 ctacaaccct ccgcatgagt gtgagtggca ctatgagcca tgtgggaacc ggagcttcga
     4441 gacctgcagg accatcaacg gcatccactc caacatctcc gtgtcctacc tggagggctg
     4501 ctacccccgg tgccccaagg acaggcccat ctatgaggag gatctgaaga agtgtgtcac
     4561 tgcagacaag tgtggctgct atgtcgagga cacccactac ccacctggag catcggttcc
     4621 caccgaggag acctgcaagt cctgcgtgtg taccaactcc tcccaagtcg tctgcaggcc
     4681 ggaagaagga aagattctta accagaccca ggatggcgcc ttctgctact gggagatctg
     4741 tggccccaac gggacggtgg agaagcactt caacatctgt tccattacgt gagaattctg
     4801 cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc
     4861 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4921 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4981 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5041 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     5101 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     5161 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5221 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5281 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5341 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5401 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5461 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5521 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     5581 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5641 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5701 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5761 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5821 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     5881 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     5941 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     6001 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     6061 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     6121 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     6181 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     6241 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     6301 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     6361 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     6421 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     6481 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     6541 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     6601 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     6661 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     6721 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     6781 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     6841 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     6901 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     6961 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7021 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7081 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7141 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7201 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7261 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7321 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7381 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7441 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7501 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7561 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7621 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7681 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7741 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7801 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7861 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7921 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7981 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8041 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8281 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8341 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8401 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8461 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8521 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8581 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8641 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8701 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8761 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8821 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8881 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8941 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9001 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9061 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9241 catttccccg aaaagtgcca cctgacgtc
//
