LOCUS       pRC1153                 5836 bp ds-DNA     circular     04-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..8
                     /label="SbfI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    9..342
                     /label="CMV enhancer (human)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        349..1709
                     /label="Actin promoter (human)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1710..1717
                     /label="NotI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1718..1749
                     /label="CleanCap-AG pT7 (full)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          1718..1749
                     /label="oRC2761.pT7-CleanCap F"
                     /note="sequence: GGCCAGTGAATTGTAATACGACTCACTATAAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1750..1794
                     /label="Moderna 5' UTR"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1795..2403
                     /label="Translation 1795-2403"
     misc_feature    1804..1869
                     /label="3xFlag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1885..2394
                     /label="NanoLuc"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2404..2504
                     /label="Moderna 3'UTR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2480..2529)
                     /label="oRC2448.Moderna3'UTRv2-pA120-mUmU R"
                     /note="sequence: mTmTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTcagactttattcaaagaccacggg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2530..2537
                     /label="NotI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      2538..2585
                     /label="T7 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2586..2591
                     /label="NheI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2592..2599
                     /label="FseI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     polyA_signal    2600..2824
                     /label="BGH pA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2825..2832
                     /label="AscI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      complement(2833..3205)
                     /label="SV40 p(A) terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(3239..3835)
                     /label="PuroR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(3836..4174)
                     /label="SV40 promoter-enhancer-origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     ori             complement(3860..3995)
                     /label="SV40 origin"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        complement(3894..4162)
                     /label="promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(3959..4174)
                     /label="SV40 enhancer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4175..4180
                     /label="SacI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      complement(4181..4207)
                     /label="KanR Terminator"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(4208..5023)
                     /label="KanR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(5024..5171)
                     /label="KanR Promoter"
                     /ApEinfo_revcolor=#82ca9d
                     /ApEinfo_fwdcolor=#82ca9d
     rep_origin      5248..5836
                     /label="ColE1"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 cctgcaggTT ATTAATAGTA ATCAATTAcg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
      421 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
      481 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
      541 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      601 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
      661 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
      721 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
      781 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
      841 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
      901 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
      961 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     1021 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     1081 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
     1141 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
     1201 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     1261 gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     1321 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     1381 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     1441 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     1501 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     1561 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     1621 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     1681 ttattgtgct gTCTCATCAT TTTGGCAAAG CggccgcGGC CAGTGAATTG TAATACGACT
     1741 CACTATAAGG AAATAAGAGA GAAAAGAAGA GTAAGAAGAA ATATAAGAGC CACCATGGga
     1801 tccgactata aggaccacga cggagactac aaggatcatg atattgatta caaggacgac
     1861 gatgacaagg gaggaggagg aagcGTCTTC ACACTCGAAG ATTTcgttgg ggactggcga
     1921 cagacagccg gctacaacct ggaccaagtc cttgaacagg gaggtgtgtc cagtttgttt
     1981 cagaatctcg gggtgtccgt aactccgatc caaaggattg tcctgagcgg tgaaaatggg
     2041 ctgaagatcg acatccatgt catcatcccg tatgaaggtc tgagcggcga ccaaatgggc
     2101 cagatcgaaa aaatttttaa ggtggtgtac cctgtggatg atcatcactt taaggtgatc
     2161 ctgcactatg gcacactggt aatcgacggg gttacgccga acatgatcga ctatttcgga
     2221 cggccgtatg aaggcatcgc cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg
     2281 tggaacggca acaaaattat cgacgagcgc ctgatcaacc ccgacggctc cctgctgttc
     2341 cgagtaacca tcaacggagt gaccggctgg cggctgtgcg aacgcattct ggcgTAGTAA
     2401 TGAGCTGGAG CCTCGGTGGC CATGCTTCTT GCCCCTTGGG CCTCCCCCCA GCCCCTCCTC
     2461 CCCTTCCTGC ACCCGTACCC CCGTGGTCTT TGAATAAAGT CTGAAAAAAA AAAAAAAAAA
     2521 AAAAAAAAAG CGGCCGCCTA GCATAACCCC TTGGGgcctc taaacgggtc ttgaggggtt
     2581 ttttggctag cggccggcct gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     2641 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     2701 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     2761 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     2821 tggcggcgcg ccactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     2881 aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg
     2941 gtatacagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     3001 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3061 ctgcaataaa caagttgggg tgggcgaaga actccagcat gagatccccg cgctggagga
     3121 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
     3181 tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaatcagg
     3241 caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg
     3301 tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtTTCcagga
     3361 aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca
     3421 gacccttgcc ctggtggtcg ggcgaCACgc cgacggtggc caggaaccac gcgggctcct
     3481 tgggccggtg cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc
     3541 tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg
     3601 gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga
     3661 gcccgacgcg cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg
     3721 gatcgacggt gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg
     3781 ccctggggac gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatgcggg
     3841 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     3901 gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     3961 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     4021 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     4081 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     4141 gagcctgggg ACTTTCCACA CCCTAACTGA CACAGAGCTC gtgttacaac caattaacca
     4201 attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     4261 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     4321 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     4381 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     4441 tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa
     4501 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     4561 gtgattgcgc ctgagcgagg cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     4621 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     4681 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc
     4741 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     4801 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     4861 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     4921 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     4981 atcgcggcct ggagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     5041 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
     5101 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt
     5161 tgaaggatca gtcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5221 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5281 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5341 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     5401 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5461 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5521 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5581 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5641 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5701 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5761 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5821 gcggagccta tggaaa
//