LOCUS       Exported                6795 bp ds-DNA    circular UNA 20-OCT-2016
DEFINITION  Concatenation of 3 sequences.
ACCESSION   urn.local...1431420252162.118
VERSION     .
KEYWORDS    pHG4
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 29 Nov 2016 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1
                     /label="origin"
                     /note="origin"
                     /note="Geneious type: origin"
     primer_bind     complement(30..46)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     oriT            complement(432..1020)
                     /direction=LEFT
                     /note="ori"
     gene            complement(1191..2051)
                     /label="bla"
                     /note="bla"
     promoter        2189..3349
                     /label="ActIp/ActII-ORF"
                     /note="ActIp/ActII-ORF promoter"
     promoter        3158..3349
                     /label="act1p"
                     /note="act1p promoter"
     misc_feature    3360..3397
                     /note="MCS"
     CDS             3398..3837
                     /gene="oriT"
                     /label="oriT"
                     /note="oriT"
     CDS             3838..4642
                     /gene="apra"
                     /label="aac(3)IV"
                     /note="apra"
     CDS             complement(4803..6662)
                     /gene="g48 Int"
                     /product="gp48"
                     /label="TG1 int"
                     /note="g48 Int"
     CDS             complement(6686..6755)
                     /gene="put attP"
                     /label="attP"
                     /note="put attP"
     CDS             complement(6769..6795)
                     /gene="'47"
                     /product="gp47 phiC31 gp26 homolog"
                     /label="'47 CDS"
                     /note="'47"
ORIGIN
        1 tctagagtcg aaagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
       61 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      121 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      181 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      241 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      301 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      361 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      421 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      481 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      541 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      601 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      661 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      721 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      781 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      841 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
      901 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
      961 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1021 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1081 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1141 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1201 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1261 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1321 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1381 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1441 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1501 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1561 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1621 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1681 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1741 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1801 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1861 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     1921 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     1981 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2041 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2101 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2161 tttccccgaa aagtgccacc tgactagtcc actgcctctc ggtaaaatcc agcaaaaatt
     2221 aatcagtgca gctcgctgca ctgattaatt tttgatcaat aggagatcgc ttgtgacggc
     2281 aagcacattg aaatctgttg agtaggcctg ttattgtcgc ccccaggaga cggagaatct
     2341 cgacgggggc gcagatgaga ttcaacttat tgggacgtgt ccatgtaatc accgatgcgg
     2401 gatgtgtaat tccgcttaaa tcctcgaagg cgacccagct cctggtgctg ctgctcctca
     2461 ggcggcacga ggtggtggga tcgggggtgc tcatcgagga gttgtgggcg gaccacccgc
     2521 cccgcagcgc catgacgacg ctgcagacgt acgtgtacca cacccgccgg ctgctggggg
     2581 agcaccgggt gacgagcgac gaccgggaat tggtcctgac ccagccgccc ggctacttcg
     2641 ccctgatcga cgaggacgaa ctcgacgtcg cggtcgccga gcgtctgatc cgcaccggcg
     2701 gccggctgct cgaggagaac cggctcgagg aggcgctcgc ctcgttggac gcgggactgg
     2761 atctctggcg aggcccggcg ctgtccaccg taccgtgcgg ccgggtgctc gaaagcaata
     2821 tcgcgcacct ggaagagctg cggctttttg gaatgcagct ccgtatcgac gcgaattggc
     2881 ggctgggcag aatagggccg atgattccgg aactccggtc cctggtaatt tcgcatccgc
     2941 tgaacgagac cctgcacgcc aaactgatgg gcgcgctctg tcagatgggc aggcgcgccg
     3001 aggcgctgga atcgtatcgg aatctccggc ggatactgtc cgacgaactg ggggtggatc
     3061 cgacgccgga aatccagcgt atgcacatgg aaattctcaa cggtgagaag gtgctcgtgt
     3121 agcaccggtc cgtgaacgcg gtggagcccc tcttaagtgt tcccctccct gcctcgtggt
     3181 ccctcacgcg ctcagctttg ggcgcccggc tcgagcggcg gtcgaaggga gatggggtgc
     3241 cgctggacgc ggcgccggtg gatccggcat cgaggggtcc cgtatcggcc ttcgagcctc
     3301 cttcgagcca cggggccgac gatgacgacg accaccggac gaacgcatcg attaattaag
     3361 cggccgccat atggaattcg gtaccgcatg cagatctagg aacttcgaag ttcccgccag
     3421 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag
     3481 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata
     3541 caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat
     3601 ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaatgca
     3661 gctcacggta actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac
     3721 gctgaaaatg ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat
     3781 aagtttatca ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg
     3841 tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt
     3901 cagcttctca accttggggt tacccccggc ggtgtgctgc tggtccacag ctccttccgt
     3961 agcgtccggc ccctcgaaga tgggccactt ggactgatcg aggccctgcg tgctgcgctg
     4021 ggtccgggag ggacgctcgt catgccctcg tggtcaggtc tggacgacga gccgttcgat
     4081 cctgccacgt cgcccgttac accggacctt ggagttgtct ctgacacatt ctggcgcctg
     4141 ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg cagcggggcc acaggcagag
     4201 cagatcatct ctgatccatt gcccctgcca cctcactcgc ctgcaagccc ggtcgcccgt
     4261 gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc caacacgacg
     4321 ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt
     4381 cttcaggatg gcaagttggt acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc
     4441 tttgccttgg cggacaggtg gctcaaggag aagagccttc agaaggaagg tccagtcggt
     4501 catgcctttg ctcggttgat ccgctcccgc gacattgtgg cgacagccct gggtcaactg
     4561 ggccgagatc cgttgatctt cctgcatccg ccagagggcg ggatgcgaag aatgcgatgc
     4621 cgctcgccag tcgattggct gagctcatga agttcctatt ccgaagttcc tattctctag
     4681 aaagtatagg aacttcgaag cagctccagc ctacacgcta ggaacagttg cttagggcaa
     4741 cgaaccgacg ccgggcacga gaaaaggggc cgaagcccct tcccgttgcg ctggtgccgc
     4801 cgtcacgccg ccgctgtgaa cccgttcagc gtctccggct ccgtctcttc ctcgaccttc
     4861 ggcttcgccc agtggatacg cacacggtcg gcgatcgggg gaacccgtcc gggccgctga
     4921 acgcccttcg gaagcttgat cacctcgatc cggtccacga agagccggac gaacgcccga
     4981 cggtcttcaa ggggcgctag cgcccaccac gaacccggtc ccgtcgggtc gctgccccgg
     5041 tcgcccagcc attcgccgat cggcagcacg ggggagtcgg cggcgtcgag ctgacgaagc
     5101 cgttcttcgg ccccttccat gcggagcgtc agcgcggctt cctcttcgag aaacgcgcgc
     5161 cgtcccatgg cggaccggta gccgccgttc cgcttgtctt cgtaaagctc ttcgagcgcc
     5221 ttcacggcgt ccgcgcgctg tgccatcagt tcggagcgct gacccttcaa ctccgggcgt
     5281 tcgtgcgtcg ctccccagcg ccgtgcggct tcgtacatga gcgctgccgt gtcgccttcg
     5341 aggtcgtcag ggtcggcagg gtcgaaggcc gtgatgcgcg cgaagatcgc gccgacgatg
     5401 tacgggtcaa ggttgtgcat cgtgatcgag catgacgacc cgtcatgaac ccggcggggg
     5461 cacttgcacg cgtacgacga cttgtgagct tgatcacctt cgcggacgtt gcccgccatg
     5521 gtcgacccgt tgctgtaacc cgtctccggg tcgagctggc cggagccgta gcagtaaagg
     5581 acgtccattg ccgacaggag cgattgcccc cggtactgac ccttcccgcg tcctcgaccc
     5641 tgaagccact cctgaagttc ccaccattcc gccgggggaa tgtacggctc gaagccgggc
     5701 agggtcagcg gctccatggt gaccgggtcg cgcctgatct tgtaatggct gaagccgccc
     5761 cgcgaaccgt cggcgcgcac cttgtatgcg atgtcagctt gatacccggc aatgcgcggg
     5821 tcgctgagta cgcgcttcaa aacgccgggg tcccaatcgg aaccggcgcg cttcttaccg
     5881 accagcgtgc cgcgcgtagg caccttgtcg cggtacagcc gctcacaaag cccgttcagc
     5941 gacccagggt gaaacgaccc ggctccgcca cccttgaatg gcgtatcgcg gtgcgtcttg
     6001 atctcctgcc acgcccagcg gattaccgcc ccttcgctgc cgtgtgcgcc ttcccaggtg
     6061 tgcgcgctgg gcaccagtcg gcgaatggca accagctttc cgccgtcttc cgggttcgga
     6121 accatttcct cgaccgtgtc gaatccgtac ggcgtcgacc ccgtgtgtcc gcccagccgc
     6181 ttcgccaatt ccttagcgtt cgacacggcg acgctcttgt tcttcgactc atcatgcgaa
     6241 gcctgaaggc gcatgatcag gtgaataagg tccatcattt cgccggggcg gaatgtgcct
     6301 tcgttcacgc tgacaatggt cacgcccagc cggagcaatt ccgtgacgac cggaataatg
     6361 tccagcggct cttcgcggct gaggcgcgaa atgtaatgaa caatgatcat gttcatttcc
     6421 cggttccggc acatgtccaa aatccggttg aactccggcc ggtcgacgcc cgtgaatgcc
     6481 gacgtgccgg gcgcttcgct gaagtgaccc agccacttca cctcgacgcc gtcgcgcgcg
     6541 tactccttcg ccagcgcctc agccttcccc cggttcgcgg cgcgctgggt ggccggtgaa
     6601 gcggtcgaac tgttctcccg ttccgccgac tgtcggtcgt agccgcctgc cagaatgacc
     6661 atgccttcaa ccttcacgct catgccgggc aggctatccc gcccaactgg gtaagagcaa
     6721 agactaacac tgttgggctg gaacggggtt gacctgcgga aacgtctctc agtcggcgcg
     6781 tgccgtgacg cttac
//
