LOCUS       Exported                6389 bp ds-DNA    circular UNA 10-OCT-2017
DEFINITION  pBF22.
ACCESSION   urn.local...1431420252257.159
VERSION     .
KEYWORDS    pHG5F
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 30 Jul 2018 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..5218
                     /label="Digestion of 22B (concatenated sequence 1)"
                     /note="Digestion of 22B (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1..3348
                     /label="Digestion of 22A (concatenated sequence 1)"
                     /note="Digestion of 22A (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1..238
                     /label="Digestion of Ligated sequences by EcoRI - Fragment 
                     2 (concatenated sequence 2)"
                     /note="Digestion of Ligated sequences by EcoRI - Fragm"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1..238
                     /label="Digestion of pIB023 Copy - Fragment 2 extraction 
                     (concatenated sequence 3)"
                     /note="Digestion of pIB023 Copy - Fragment 2 extractio"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    239..3343
                     /label="Digestion of pIB023 Copy - Fragment 2 - 
                     lyuEFyU9jS00Beo2DA2I1/GnynA.1386062625056.25 extraction 
                     (concatenated sequence 1)"
                     /note="Digestion of pIB023 Copy - Fragment 2 - lyuEFyU"
                     /note="Geneious type: Concatenated sequence"
     gene            complement(421..1009)
                     /note="ori"
     gene            complement(1180..2040)
                     /label="bla"
                     /note="bla"
     primer_bind     2142..2180
                     /label="05"
                     /note="05"
     primer_bind     2142..2180
                     /label="bf005"
                     /note="bf005"
     misc_feature    2178..3138
                     /label="ActII ORF4"
                     /note="ActII ORF4"
                     /note="Geneious type: ORF"
     primer_bind     2178..2195
                     /label="BF009"
                     /note="BF009"
     primer_bind     2178..2195
                     /label="9"
                     /note="9"
     gene            2344..3111
                     /label="actIP/actII ORF4"
                     /note="actIP/actII ORF4"
     misc_feature    3141..4235
                     /note="sequencing 24GD23"
     promoter        3147..3338
     misc_feature    complement(3319..3338)
                     /label="BF010"
                     /note="BF010"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    3341..3386
                     /note="MCS"
     misc_feature    3344..3345
                     /label="Ligation"
                     /note="Ligation"
                     /note="Geneious type: ligation"
     misc_feature    3346..3348
                     /label="Digestion of PCR - Fragment 2 extraction 
                     (concatenated sequence 2)"
                     /note="Digestion of PCR - Fragment 2 extraction (conca"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    3346..3348
                     /note="Geneious type: Editing History Insertion"
     misc_feature    3387..5200
                     /label="pMS98  (reversed) (concatenated sequence 2)"
                     /note="pMS98 (reversed) (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_signal     complement(3387..3391)
                     /gene="'BTattP"
                     /label="'BTattP misc signal"
                     /note="'BTattP misc signal"
     CDS             3393..5036
                     /gene="Int"
                     /product="protein_id=""AFU62167.1"""
                     /label="SV1 int/attP"
                     /note="SV1 Int"
     misc_signal     complement(5056..5085)
                     /gene="(null)"
                     /label="(null) misc signal"
                     /note="(null) misc signal"
     misc_signal     complement(5142..5183)
                     /gene="(null)"
                     /label="SV1 attP"
                     /note="SV1 attP"
     primer_bind     complement(5183..5200)
                     /label="pr"
                     /note="pr"
     misc_feature    5200..5201
                     /label="origin"
                     /note="origin"
                     /note="Geneious type: origin"
     misc_signal     5200
                     /gene="'ter?"
                     /label="'ter? misc signal"
                     /note="'ter? misc signal"
     misc_feature    5201..5218
                     /label="Digestion of 22A (concatenated sequence 1)"
                     /note="Digestion of 22A (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    5205..5218
                     /label="Digestion of Ligated sequences by EcoRI - Fragment 
                     2 (concatenated sequence 2)"
                     /note="Digestion of Ligated sequences by EcoRI - Fragm"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    5205..5218
                     /label="Digestion of pIB023 Copy - Fragment 2 extraction 
                     (concatenated sequence 3)"
                     /note="Digestion of pIB023 Copy - Fragment 2 extractio"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    5218..5219
                     /label="CATG"
                     /note="CATG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    5218..5219
                     /label="TT"
                     /note="TT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    5219..6389
                     /label="Ligated sequences (concatenated sequence 2)"
                     /note="Ligated sequences (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    5219..5458
                     /label="OriT (concatenated sequence 1)"
                     /note="OriT (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     CDS             5304..5413
                     /gene="oriT"
                     /label="oriT"
                     /note="oriT"
     misc_feature    5459..6389
                     /label="KANA  (reversed) (concatenated sequence 2)"
                     /note="KANA (reversed) (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    5465..5595
                     /gene="aphIIp"
                     /label="aphIIp misc"
                     /note="aphIIp misc"
     CDS             5595..6389
                     /gene="aphII"
                     /label="aphII"
                     /note="aphII"
ORIGIN
        1 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
       61 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      121 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      181 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      241 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      301 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      361 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      421 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      481 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      541 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      601 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      661 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      721 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      781 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      841 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      901 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      961 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1021 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1081 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1141 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1201 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1261 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1321 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1381 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1441 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1501 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1561 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1621 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1681 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1741 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1801 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1861 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1921 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1981 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2041 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2101 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2161 agtgccacct gactagtcca ctgcctctcg gtaaaatcca gcaaaaatta atcagtgcag
     2221 ctcgctgcac tgattaattt ttgatcaata ggagatcgct tgtgacggca agcacattga
     2281 aatctgttga gtaggcctgt tattgtcgcc cccaggagac ggagaatctc gacgggggcg
     2341 cagatgagat tcaacttatt gggacgtgtc catgtaatca ccgatgcggg atgtgtaatt
     2401 ccgcttaaat cctcgaaggc gacccagctc ctggtgctgc tgctcctcag gcggcacgag
     2461 gtggtgggat cgggggtgct catcgaggag ttgtgggcgg accacccgcc ccgcagcgcc
     2521 atgacgacgc tgcagacgta cgtgtaccac acccgccggc tgctggggga gcaccgggtg
     2581 acgagcgacg accgggaatt ggtcctgacc cagccgcccg gctacttcgc cctgatcgac
     2641 gaggacgaac tcgacgtcgc ggtcgccgag cgtctgatcc gcaccggcgg ccggctgctc
     2701 gaggagaacc ggctcgagga ggcgctcgcc tcgttggacg cgggactgga tctctggcga
     2761 ggcccggcgc tgtccaccgt accgtgcggc cgggtgctcg aaagcaatat cgcgcacctg
     2821 gaagagctgc ggctttttgg aatgcagctc cgtatcgacg cgaattggcg gctgggcaga
     2881 atagggccga tgattccgga actccggtcc ctggtaattt cgcatccgct gaacgagacc
     2941 ctgcacgcca aactgatggg cgcgctctgt cagatgggca ggcgcgccga ggcgctggaa
     3001 tcgtatcgga atctccggcg gatactgtcc gacgaactgg gggtggatcc gacgccggaa
     3061 atccagcgta tgcacatgga aattctcaac ggtgagaagg tgctcgtgta gcaccggtcc
     3121 gtgaacgcgg tggagcccct cttaagtgtt cccctccctg cctcgtggtc cctcacgcgc
     3181 tcagctttgg gcgcccggct cgagcggcgg tcgaagggag atggggtgcc gctggacgcg
     3241 gcgccggtgg atccggcatc gaggggtccc gtatcggcct tcgagcctcc ttcgagccac
     3301 ggggccgacg atgacgacga ccaccggacg aacgcatcga ttaattaagc ggccgccata
     3361 tgtctagagg taccgagctc gctagcagat ctatgaaacg agacctacca agcacgttcc
     3421 gcggctcccg cacgccgggc gagccgtggc tcggctacat cagggtcagc acatggcgcg
     3481 aggagaagat cagcccggag ctacagcaat ccgcgatcga gtcatgggcc gcccgaacgg
     3541 gacgtcggat cgtcgactgg atcgttgacc tcgacgctac gggccgcaac ttcaagcgga
     3601 agatcatggg cggcatccag cgcgtcgagg gccgcgaggt cgtcggaatc gccgtatgga
     3661 agttcagccg cttcggccgg aacgacctgg gcatcgctat caacctcgcc cgcctcgaac
     3721 aggccggcgg agacctcgcg tccgccacgg aagaagtcga cgcccgcacg gccgtcggac
     3781 gcttcaaccg cgcgattctg ttcgacctcg ccgtattcga gtcggatcgg gccggcgaac
     3841 agtggaagga gacgcacgcc caccgtcggg ccctgaaact ccccgccacc gggcgtcagc
     3901 ggttcggcta cgtgtggcac ccgaggcgcg tacccgacct caccgcgccg ggcgggttcc
     3961 gcctgcaaga ggagcgctac gaacggcacc cagagttcgc ccccgtggcg gccgaactct
     4021 acgagcgaaa gctcgccgga caggggttct cccagctcgc ctactggctg aacgacgaac
     4081 tactcatccc caccacacgg ggcaaccgct ggggcaccaa caccgttcag cgctacctcg
     4141 actccggatt cgccgccggc ctgctacgcg tccacgaccc ggagtgccgg tgcaagctgg
     4201 gacaggacca cttctctgcg tgcaaagaaa accggtggct ctggctcccc ggggcacagc
     4261 cggcgctcat cgttcccgag cagtggaagg agtacggggc gcacagagag cagacgcgca
     4321 agacgccccc gcgcgcccgc cgggcctcat acccgaccag cggcatcatg cggcacggac
     4381 actgccgcgg gacggccgtt gcgcgatccg gccgcgacgg gaagggcgga ttcgttccgg
     4441 gccacgtgtt cgtgtgcttc aaccgcagga acaaggggaa gtcggcctgt gagcccggcc
     4501 tctacgtgcg ccgcgacgaa gtcgaggccg aggtgttgaa gtggctcgcc gacacggtcg
     4561 ccgacgacat cgacaacgcg ccggcccttc ccgctcagcg caccgcgccg ggcaccgcgc
     4621 cggacccgcg ggcccgcctg gtcgaggagc gcacccgcac ggaagccgag ttggccaaga
     4681 tcgagggtgc actcgaccgt ctcgtcacgg attacgcgct ggacccggac aagtacccgg
     4741 cggacacgtt cgggcgcgta cgcgaccagc tactcggtaa gaagggcgac atcatcaagc
     4801 acttgaagtc gctcagcgag gttgaagtcg cgcccacgcg ggaggagttc cgccccctga
     4861 tcgtcgggct cttgcaggag tgggacatcc tgcacaccac cgagaagaac gccatcctgc
     4921 gccgcctgct gcgccgtctg gtcatccaca accgtaagag cgatcagggc gcacagtgga
     4981 gtgtcgtgcg cagcttcgag tttcatcccg tgtgggagcc cgacccctgg tcttgaccgc
     5041 tggagcgcaa accatgcagg cgcgcttgat tgtttctccg cctgctgcca ccctctgaaa
     5101 accgcacctc agtgcaggga gagggggaac gatgctcgcg agtcctttag agacactgac
     5161 ccacgtcagt ggatctaaag gaccacatcg gagcgcgaag ctagagtcga cctgcaggcg
     5221 gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt
     5281 ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc
     5341 gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct
     5401 ctgcgaggct ggcgggaact ctacacatcg aattcctgca gcccggggga tccactaggg
     5461 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     5521 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat
     5581 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     5641 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     5701 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     5761 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     5821 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     5881 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     5941 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     6001 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     6061 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     6121 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     6181 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     6241 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     6301 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     6361 tctatcgcct tcttgacgag ttcttctga
//
