LOCUS       Exported                7090 bp ds-DNA    circular UNA 27-APR-2017
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...1431420252387.232
VERSION     .
KEYWORDS    pHG6
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 18 May 2017 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          3380..4765
                     /organism="Saccharopolyspora erythraea NRRL 2338"
                     /strain="NRRL 2338"
                     /mol_type="genomic DNA"
                     /label="source Saccharopolyspora erythraea NRRL 2338"
                     /note="source Saccharopolyspora erythraea NRRL 2338"
                     /db_xref="taxon:405948"
     misc_feature    1..4777
                     /label="Digestion of 24c (concatenated sequence 1)"
                     /note="Digestion of 24c (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1..3348
                     /label="Digestion of Ligated sequences 4 extraction 
                     (concatenated sequence 1)"
                     /note="Digestion of Ligated sequences 4 extraction (co"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1..3348
                     /label="Digestion of pBF24B (concatenated sequence 1)"
                     /note="Digestion of pBF24B (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1..3348
                     /label="Digestion of pIB023 Copy - Fragment 2 (concatenated
                     sequence 1)"
                     /note="Digestion of pIB023 Copy - Fragment 2 (concaten"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1..238
                     /label="Digestion of Ligated sequences by EcoRI - Fragment 
                     2 (concatenated sequence 2)"
                     /note="Digestion of Ligated sequences by EcoRI - Fragm"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    complement(421..1009)
                     /note="Ori"
     gene            complement(1180..2040)
                     /label="bla"
                     /note="bla"
     promoter        complement(2041..2145)
                     /note="Amp promoter"
     primer_bind     2142..2180
                     /label="bf005"
                     /note="bf005"
     primer_bind     2142..2180
                     /label="05"
                     /note="05"
     misc_feature    2178..3138
                     /label="ActII ORF4"
                     /note="ActII ORF4"
                     /note="Geneious type: ORF"
     promoter        2178..3138
                     /note="ACTII-ORT4"
     primer_bind     2178..2195
                     /label="9"
                     /note="9"
     primer_bind     2178..2195
                     /label="BF009"
                     /note="BF009"
     misc_feature    complement(2178..2186)
                     /label="BF008"
                     /note="BF008"
                     /note="Geneious type: primer_bind_reverse"
     promoter        3147..3338
     promoter        3147..3338
                     /note="act1p"
     misc_feature    complement(3319..3338)
                     /label="BF010"
                     /note="BF010"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    3341..3379
                     /note="MCS"
     misc_feature    3346..3348
                     /note="Geneious type: Editing History Insertion"
     misc_feature    3380..4765
                     /label="ner erm e (concatenated sequence 2)"
                     /note="ner erm e (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     gene            complement(3434..4579)
                     /gene="ermE"
                     /locus_tag="SACE_0733"
                     /label="ermE gene"
                     /note="ermE"
                     /db_xref="GeneID:4940594"
     misc_feature    complement(3725..4483)
                     /gene="ermE"
                     /locus_tag="SACE_0733"
                     /label="ermE misc"
                     /note="ermE misc"
                     /note="Ribosomal RNA adenine dimethylase; Region: RrnaAD; 
                     pfam00398"
                     /db_xref="CDD:215900"
     misc_feature    complement(4193..4387)
                     /gene="ermE"
                     /locus_tag="SACE_0733"
                     /label="ermE misc"
                     /note="ermE misc"
                     /note="S-adenosylmethionine-dependent methyltransferases 
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes 
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a 
                     substrate for methyltransfer, creating the product 
                     S-adenosyl-L-homocysteine (AdoHcy); Region: AdoMet_MTases; 
                     cd02440"
                     /db_xref="CDD:100107"
     misc_feature    complement(4232..4240)
                     /gene="ermE"
                     /locus_tag="SACE_0733"
                     /label="ermE misc"
                     /note="ermE misc"
                     /note="S-adenosylmethionine binding site [chemical 
                     binding]; other site"
                     /db_xref="CDD:100107"
     misc_feature    complement(4307..4312)
                     /gene="ermE"
                     /locus_tag="SACE_0733"
                     /label="ermE misc"
                     /note="ermE misc"
                     /note="S-adenosylmethionine binding site [chemical 
                     binding]; other site"
                     /db_xref="CDD:100107"
     misc_feature    complement(4358..4378)
                     /gene="ermE"
                     /locus_tag="SACE_0733"
                     /label="ermE misc"
                     /note="ermE misc"
                     /note="S-adenosylmethionine binding site [chemical 
                     binding]; other site"
                     /db_xref="CDD:100107"
     misc_feature    4766..4777
                     /label="Digestion of pBF24B (concatenated sequence 1)"
                     /note="Digestion of pBF24B (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    4770..4777
                     /label="Digestion of Ligated sequences by EcoRI - Fragment 
                     2 (concatenated sequence 2)"
                     /note="Digestion of Ligated sequences by EcoRI - Fragm"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    4770..4777
                     /label="Digestion of Ligated sequences 4 extraction 
                     (concatenated sequence 1)"
                     /note="Digestion of Ligated sequences 4 extraction (co"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    4770..4777
                     /label="Digestion of pIB023 Copy - Fragment 2 (concatenated
                     sequence 1)"
                     /note="Digestion of pIB023 Copy - Fragment 2 (concaten"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    4777..4778
                     /label="TT"
                     /note="TT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    4777..4778
                     /label="TGCA"
                     /note="TGCA"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    4778..7090
                     /label="Ligated sequences (concatenated sequence 2)"
                     /note="Ligated sequences (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     CDS             4863..4972
                     /gene="oriT"
                     /label="oriT"
                     /note="oriT"
     CDS             complement(4950..4972)
                     /gene="KA074v2"
                     /note="KA074v2"
     misc_feature    complement(5006..5014)
                     /label="BF006"
                     /note="BF006"
                     /note="Geneious type: primer_bind_reverse"
     CDS             complement(5023..5042)
                     /gene="usp"
                     /note="usp"
     primer_bind     complement(5023..5042)
                     /label="07"
                     /note="07"
     misc_feature    5043..5162
                     /label="Ligated sequences (concatenated sequence 2)"
                     /note="Ligated sequences (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     CDS             complement(5181..6965)
                     /gene="int"
                     /label="phiBT1 int/attP"
                     /note="BT1 int"
     gene            complement(7009..7080)
                     /note="BT1 attP"
ORIGIN
        1 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
       61 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      121 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      181 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      241 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      301 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      361 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      421 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      481 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      541 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      601 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      661 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      721 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      781 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      841 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      901 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      961 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1021 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1081 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1141 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1201 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1261 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1321 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1381 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1441 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1501 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1561 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1621 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1681 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1741 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1801 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1861 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1921 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1981 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2041 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2101 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2161 agtgccacct gactagtcca ctgcctctcg gtaaaatcca gcaaaaatta atcagtgcag
     2221 ctcgctgcac tgattaattt ttgatcaata ggagatcgct tgtgacggca agcacattga
     2281 aatctgttga gtaggcctgt tattgtcgcc cccaggagac ggagaatctc gacgggggcg
     2341 cagatgagat tcaacttatt gggacgtgtc catgtaatca ccgatgcggg atgtgtaatt
     2401 ccgcttaaat cctcgaaggc gacccagctc ctggtgctgc tgctcctcag gcggcacgag
     2461 gtggtgggat cgggggtgct catcgaggag ttgtgggcgg accacccgcc ccgcagcgcc
     2521 atgacgacgc tgcagacgta cgtgtaccac acccgccggc tgctggggga gcaccgggtg
     2581 acgagcgacg accgggaatt ggtcctgacc cagccgcccg gctacttcgc cctgatcgac
     2641 gaggacgaac tcgacgtcgc ggtcgccgag cgtctgatcc gcaccggcgg ccggctgctc
     2701 gaggagaacc ggctcgagga ggcgctcgcc tcgttggacg cgggactgga tctctggcga
     2761 ggcccggcgc tgtccaccgt accgtgcggc cgggtgctcg aaagcaatat cgcgcacctg
     2821 gaagagctgc ggctttttgg aatgcagctc cgtatcgacg cgaattggcg gctgggcaga
     2881 atagggccga tgattccgga actccggtcc ctggtaattt cgcatccgct gaacgagacc
     2941 ctgcacgcca aactgatggg cgcgctctgt cagatgggca ggcgcgccga ggcgctggaa
     3001 tcgtatcgga atctccggcg gatactgtcc gacgaactgg gggtggatcc gacgccggaa
     3061 atccagcgta tgcacatgga aattctcaac ggtgagaagg tgctcgtgta gcaccggtcc
     3121 gtgaacgcgg tggagcccct cttaagtgtt cccctccctg cctcgtggtc cctcacgcgc
     3181 tcagctttgg gcgcccggct cgagcggcgg tcgaagggag atggggtgcc gctggacgcg
     3241 gcgccggtgg atccggcatc gaggggtccc gtatcggcct tcgagcctcc ttcgagccac
     3301 ggggccgacg atgacgacga ccaccggacg aacgcatcga ttaattaagg taccgagctc
     3361 catatgcctc agcgcatgct tcctctcgac caacaggccg ctcgacggcc tgccccgttc
     3421 cgcgtgccgg ggactaccgc tgcccgggtc cgccgcgtcg ccctggacgt ccgccggtcc
     3481 gtccaccccc ggacggcccc cgccgcccgc cttggtcccc ctgctcgcgc tcgccggttc
     3541 gtccgtcgtc gcgattgcgt ccgctgctgc gacgatcgcc gtggccgctg gcttgccggt
     3601 ccctgtggtc gcggccgccg gttcgccgat cgccgcgatc ctgcccgccg gttcgccggt
     3661 ccccgtggtc ccgtccgccg gttcgcctgc cccgctgggg ctgcccaccc ctcggttcgt
     3721 tcttctgatc cagccgctcg aacagccgga gccactgctc cggccggacg taggcgacca
     3781 cggcgccgcc cccgaccccc gcgtggtcga gcgccgcctc gacgcggcgc ctcgggtact
     3841 tgcgcagaag cgacgcctgg atgttgccgc cgacgccggt gaagcacagc tcgaccatcg
     3901 actcgtagcg ttcgagcgcc gcgccttcca gcagcggttc ggcgcgcctg cgcagccgca
     3961 tgatcgccga gtcgaccttg ggcaccggct tgaacagccg gcggtcgacc ttctcgacga
     4021 actcccactc gaacagcggc caggtcatca ccgtgaggcg gctccagcgg ccgtaatcgc
     4081 cggtccgctt ccgggcgaac tccagctgcg tgaccatcgt cgccgtctcg atcgtcggcg
     4141 cctccaggca ccagtccacg atcgccgagg tgatgccgta ggggatcgcg ccgacgaagg
     4201 cgaacggctc gggcggcggt tcggcggtga ggaagtcggc gttgacgact tcgatgttcg
     4261 ggtggccgga aagcttctcc cgcaacgact tcgccagccg ggggtcgatc tcgtacgacg
     4321 tcacctgacg cgcgcggtcg gcgagttccc tggtgagcag cccttcgccg gggccggctt
     4381 ccagcaccgg cagatcgggc cgcagctcgg ctgtctcggc gatgcgcgcg atggtcttgc
     4441 ggtcgcggag gaagttctgc ccgaactggc gccggttgcg gtcccgctcg gtacggccca
     4501 gcaccggccg gttctggttg gggtgctgcc gatcctggtt gcggcgacgc gggcgcggct
     4561 gctcgtccga actgctcacc gctggatcct accaaccggc acgattgtcc agcccacaac
     4621 agcatcgcgg tgccacgtgt ggaccgcgtc ggtcagatcc tccccgcacc tctcgccagc
     4681 cgtcaagatc gaccgcgtgc acctgcgatc gccgatcaac cgcgactagc atcgggcgca
     4741 agccgccact cgaacggaca ctcgcctaga gtcgacccgg tatatccatc ctttttcgca
     4801 cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt
     4861 cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc
     4921 ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg gcgggaactt
     4981 cgaagttcct atactttcta gagaatagga acttcgaact gcaggtcgac ggatccccgg
     5041 aatatcaagc ttatcgatac cgtcgacctc gagggggatc cactagtggt gacgaaggaa
     5101 ctactagtta gcctaactaa cgatttcaag gccaaaaaag gggccgaacc gtccgccggc
     5161 tcgacccctc ccgctgtgcg ctacagcgcc gcaagctccc gctcagtggc ttcgctcgct
     5221 tcgtcttcct ccttcagcag ctccgcccac ttgagcgtca cacggtcctt cagggggatg
     5281 tatttcttcg tctcagggtg gcgcccccgg tcgaccatga cggagtcaag gaagaagctc
     5341 aggaactccc gacgctcgta cacgtcccag cttgcccaga tgcccccttc ggccgtcggg
     5401 tcttcgccgc tgaaccactc agacggaacg cgggtgctgc cgttcatctt ctcgtcaagc
     5461 tcagcgagtc gggtcgtgca ctccgcttcg taggaccggt attgcagcac cgttgagcgc
     5521 cacgtttcca gctcttcacg cccgacgtac agaccggcct tacggtccgc ctgaaggtcc
     5581 ttgatggagc gccgcacgtt gtctaggtgc gcctgttgtt cgcgccgctc atcggccacc
     5641 cccgctaggt cgtgctgaag ggcgaagcgc tccgcagcgg cggcaatcca tgcctgatcg
     5701 tgttcatctt ccatgtcggc tgtgcggagc cgtgcccaca ccttcgaagc aacgaattcg
     5761 tccagttcgc tgcgcttgac cgacaagccg ccgtgcccct tcgggttggc gcacatgtaa
     5821 ttgccttcgg caaggtcgcc gttgcgctta cggccaccct gggactgacc cattgagccg
     5881 ccgcagattc ggcaccccag gaaacgccac ccgctcagaa gcgtcggttc gacttcggcc
     5941 ccaggcttcc ggtcgctgag attcttcccg gaacgcttct cttgaagctc aagccacttc
     6001 gagccgctga gaatgcccgt gtggggcgtt agcggcttgc cgccggggtc gcgccgtatg
     6061 acgttgatgt gcgccttacc gtgcttcaca cgctcgaatg cgaaaccgcc gattgcggga
     6121 tggttgagaa tccatcggac cgtttgagcg cgccacatga tcggcttttc agcgccgttc
     6181 aggcgacgtg ccttgatgga cgcaagacgc ttttccgtgg cgcgtctctc agccattccg
     6241 ggcgacggga tcttttcctt ctcgaaggtc gttgcaatgg cgttgtcgga cacgccttcg
     6301 aacgacattt tcgccatgcg ctcaactagc tcgacgtgat ccgggttgtc ttcgtccggc
     6361 tcaagaacgg agatcacgag attatcgacc ttcttgcgca cggcgcgcat tccgaacggg
     6421 gcggaagacg agtgaacgcc acccagcgcg gcaatctcgt ctttcgcacc cttcaggcgc
     6481 tccgccttca ggtcactgtc ctgtttcgca agggcagcga tcagcgcgaa aatggcgacg
     6541 ccgatagggg tagacgtgtc aaggaacggc tcaagaaccg acatgaagcg cacgccgtgc
     6601 ttcttcaatt cgttgtcgat ttcgagcgcg tcatgggcgc ccttgcgagt gagccgggaa
     6661 agctcattca caacgacaac gtcaccttcg ccggcgcgga cttcgcccat catgcgctcg
     6721 aagtcggcac gggtcacgtt cgggtcccag ccggagcgcc cgacgtccac gaactcacct
     6781 gccacgaccc agcgcgcacc cccctgggcg ttgcgagacg cgaccaacgc acgaccggcc
     6841 gctagctgtg cttcggtcga cacgtctgag ccgtcggacc ggcccttaga ctgacgcgcg
     6901 tacaggaaga cgcgaacagt gtctagaagg tgctcaggga cgtcgggagc gatgaagggc
     6961 gacatgccta gatcttggct catgggcgct gacctgcgct ttcttttggg aacattggtg
     7021 gtgctgagta gtttcccatg gatcactgtc cagagacaac aacccagcac ccgaaacgtc
     7081 tgaagccccg
//
