LOCUS       Exported                6984 bp ds-DNA    circular SYN 23-NOV-2016
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pHG7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 29 Nov 2016 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     oriT            442..551
                     /note="incP origin of transfer"
     CDS             complement(651..662)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     protein_bind    733..832
                     /bound_moiety="phage phi-C31 integrase"
                     /note="phage phi-C31 attP"
                     /note="attachment site of phage phi-C31"
     CDS             842..2659
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /note="phage phi-C31 integrase"
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     primer_bind     complement(2680..2696)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2704..2720
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2728..2758)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2773..2794
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3082..3670)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3841..4701)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4702..4806)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        5005..5772
                     /note="actII-orf4"
     promoter        5808..5999
                     /note="act1p"
     gene            complement(6066..80)
                     /note="Hyg B"
ORIGIN
        1 aattcctggt cgttccgcag gctcgcgtag gaatcatccg aatcaatacg gtcgagaagt
       61 aacagggatt cttgtgtcac agcggacctc tattcacagg gtacgggccg gcttaattcc
      121 gcacggccgg tcgcgacacg gcctgtccgc accgcggatc aggcgttgac gatgacgggc
      181 tggtcggcca cgtcggggac gacggggagt caggcaacta ttattgaagc atttatcagg
      241 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
      301 ttccgcgcac atttccccga aaagtgccac ctgactagcg ttcgtctgga aggcagtaca
      361 ccttgatagg tgggctgccc ttcctggttg gcttggtttc atcagccatc cgcttgccct
      421 catctgttac gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg
      481 ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac
      541 ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc
      601 aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgcg acgcccctct
      661 atggcccgta ctgacggaca caccgaagcc ccggcggcaa ccctcagcgg atgccccggg
      721 gcttcacgtt ttcccaggtc agaagcggtt ttcgggagta gtgccccaac tggggtaacc
      781 tttgagttct ctcagttggg ggcgtagggt cgccgacatg acacaagggg ttgtgaccgg
      841 ggtggacacg tacgcgggtg cttacgaccg tcagtcgcgc gagcgcgaga actcgagcgc
      901 agcaagccca gcgacacagc gtagcgccaa cgaagacaag gcggccgacc ttcagcgcga
      961 agtcgagcgc gacgggggcc ggttcaggtt cgtcgggcat ttcagcgaag cgccgggcac
     1021 gtcggcgttc gggacggcgg agcgcccgga gttcgaacgc atcctgaacg aatgccgcgc
     1081 cgggcggctc aacatgatca ttgtctatga cgtgtcgcgc ttctcgcgcc tgaaggtcat
     1141 ggacgcgatt ccgattgtct cggaattgct cgccctgggc gtgacgattg tttccactca
     1201 ggaaggcgtc ttccggcagg gaaacgtcat ggacctgatt cacctgatta tgcggctcga
     1261 cgcgtcgcac aaagaatctt cgctgaagtc ggcgaagatt ctcgacacga agaaccttca
     1321 gcgcgaattg ggcgggtacg tcggcgggaa ggcgccttac ggcttcgagc ttgtttcgga
     1381 gacgaaggag atcacgcgca acggccgaat ggtcaatgtc gtcatcaaca agcttgcgca
     1441 ctcgaccact ccccttaccg gacccttcga gttcgagccc gacgtaatcc ggtggtggtg
     1501 gcgtgagatc aagacgcaca aacaccttcc cttcaagccg ggcagtcaag ccgccattca
     1561 cccgggcagc atcacggggc tttgtaagcg catggacgct gacgccgtgc cgacccgggg
     1621 cgagacgatt gggaagaaga ccgcttcaag cgcctgggac ccggcaaccg ttatgcgaat
     1681 ccttcgggac ccgcgtattg cgggcttcgc cgctgaggtg atctacaaga agaagccgga
     1741 cggcacgccg accacgaaga ttgagggtta ccgcattcag cgcgacccga tcacgctccg
     1801 gccggtcgag cttgattgcg gaccgatcat cgagcccgct gagtggtatg agcttcaggc
     1861 gtggttggac ggcagggggc gcggcaaggg gctttcccgg gggcaagcca ttctgtccgc
     1921 catggacaag ctgtactgcg agtgtggcgc cgtcatgact tcgaagcgcg gggaagaatc
     1981 gatcaaggac tcttaccgct gccgtcgccg gaaggtggtc gacccgtccg cacctgggca
     2041 gcacgaaggc acgtgcaacg tcagcatggc ggcactcgac aagttcgttg cggaacgcat
     2101 cttcaacaag atcaggcacg ccgaaggcga cgaagagacg ttggcgcttc tgtgggaagc
     2161 cgcccgacgc ttcggcaagc tcactgaggc gcctgagaag agcggcgaac gggcgaacct
     2221 tgttgcggag cgcgccgacg ccctgaacgc ccttgaagag ctgtacgaag accgcgcggc
     2281 aggcgcgtac gacggacccg ttggcaggaa gcacttccgg aagcaacagg cagcgctgac
     2341 gctccggcag caaggggcgg aagagcggct tgccgaactt gaagccgccg aagccccgaa
     2401 gcttcccctt gaccaatggt tccccgaaga cgccgacgct gacccgaccg gccctaagtc
     2461 gtggtggggg cgcgcgtcag tagacgacaa gcgcgtgttc gtcgggctct tcgtagacaa
     2521 gatcgttgtc acgaagtcga ctacgggcag ggggcaggga acgcccatcg agaagcgcgc
     2581 ttcgatcacg tgggcgaagc cgccgaccga cgacgacgaa gacgacgccc aggacggcac
     2641 ggaagacgta gcggcgtagc gaagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     2701 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     2761 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     2821 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     2881 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2941 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3001 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3061 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3121 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3181 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3241 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3301 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3361 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3421 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3481 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     3541 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3601 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3661 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3721 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3781 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3841 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3901 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3961 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4021 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4081 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4141 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4201 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4261 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4321 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4381 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4441 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4501 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4561 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4621 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4681 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4741 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4801 tccgcgcaca tttccccgaa aagtgccacc tgactagtcc actgcctctc ggtaaaatcc
     4861 agcaaaaatt aatcagtgca gctcgctgca ctgattaatt tttgatcaat aggagatcgc
     4921 ttgtgacggc aagcacattg aaatctgttg agtaggcctg ttattgtcgc ccccaggaga
     4981 cggagaatct cgacgggggc gcagatgaga ttcaacttat tgggacgtgt ccatgtaatc
     5041 accgatgcgg gatgtgtaat tccgcttaaa tcctcgaagg cgacccagct cctggtgctg
     5101 ctgctcctca ggcggcacga ggtggtggga tcgggggtgc tcatcgagga gttgtgggcg
     5161 gaccacccgc cccgcagcgc catgacgacg ctgcagacgt acgtgtacca cacccgccgg
     5221 ctgctggggg agcaccgggt gacgagcgac gaccgggaat tggtcctgac ccagccgccc
     5281 ggctacttcg ccctgatcga cgaggacgaa ctcgacgtcg cggtcgccga gcgtctgatc
     5341 cgcaccggcg gccggctgct cgaggagaac cggctcgagg aggcgctcgc ctcgttggac
     5401 gcgggactgg atctctggcg aggcccggcg ctgtccaccg taccgtgcgg ccgggtgctc
     5461 gaaagcaata tcgcgcacct ggaagagctg cggctttttg gaatgcagct ccgtatcgac
     5521 gcgaattggc ggctgggcag aatagggccg atgattccgg aactccggtc cctggtaatt
     5581 tcgcatccgc tgaacgagac cctgcacgcc aaactgatgg gcgcgctctg tcagatgggc
     5641 aggcgcgccg aggcgctgga atcgtatcgg aatctccggc ggatactgtc cgacgaactg
     5701 ggggtggatc cgacgccgga aatccagcgt atgcacatgg aaattctcaa cggtgagaag
     5761 gtgctcgtgt agcaccggtc cgtgaacgcg gtggagcccc tcttaagtgt tcccctccct
     5821 gcctcgtggt ccctcacgcg ctcagctttg ggcgcccggc tcgagcggcg gtcgaaggga
     5881 gatggggtgc cgctggacgc ggcgccggtg gatccggcat cgaggggtcc cgtatcggcc
     5941 ttcgagcctc cttcgagcca cggggccgac gatgacgacg accaccggac gaacgcatcg
     6001 attaattaag cggccgccat atgtctagag gtaccgctag cgcatgcaga tctcggcccg
     6061 gggcgtcagg cgccgggggc ggtgtccggc ggcccccaga ggaactgcgc cagttcctcc
     6121 ggatcggtga agccggagag atccagcggg gtctcctcga acacctcgaa gtcgtgcagg
     6181 aaggtgaagg cgagcagttc gcgggcgaag tcctcggtcc gcttccactg cgccccgtcg
     6241 agcagcgcgg ccaggatctc gcggtcgccc cggaaggcgt tgagatgcag ttgcaccagg
     6301 ctgtagcggg agtctcccgc atagacgtcg gtgaagtcga cgatcccggt gacctcggtc
     6361 gcggccaggt ccacgaagat gttggtcccg tgcaggtcgc cgtggacgaa ccggggttcg
     6421 cggccggcca gcagcgtgtc cacgtccggc agccagtcct ccaggcggtc cagcagccgg
     6481 ggcgagaggt agccccaccc gcggtggtcc tcgacggtcg ccgcgcggcg ttcccgcagc
     6541 agttccggga agacctcgga atggggggtg agcacggtgt tcccggtcag cggcaccctg
     6601 tgcagccggc cgagcacccg gccgagttcg cgggccaggg cgagcagcgc gttccggtcg
     6661 gtcgtgccgt ccatcgcgga ccgccaggtg gtgccggtca tccggctcat caccaggtag
     6721 ggccacggcc aggctccggt gccgggccgc agctcgccgc ggccgaggag gcggggcacc
     6781 ggcaccgggg cgtccgccag gaccgcgtac gcctccgact ccgacgcgag gctctccgga
     6841 ccgcaccagt gctcgccgaa cagcttgatc accgggtcgg gctcgccgac cagtacgggg
     6901 ttggtgctct cgccgggcac ccgcagcacc ggcggcaccg gcagcccgag ctcctccagg
     6961 gctcggcggg ccagcggctc ccag
//
