LOCUS       pCMV-dCas9:nls:g        9631 bp    DNA     circular SYN 23-JUN-2022
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13461718|
COMMENT     VNTDBDATE|-14449639|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCMV-dCas9:nls:gs:DHFR[C174]|
COMMENT     VNTAUTHORNAME|erik|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     source          1..9631
                     /organism="pcDNA3"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(5184..5201)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(5185..5202)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(5221..5238)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      5224..5451
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      5514..5820
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(5934..5954)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(5940..6156)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        5952..6221
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      6120..6197
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    6182..6201
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             6336..7130
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            6339..7127
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      7307..7426
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    7395..7414
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(7470..7488)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(7487..7509)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(7523..7552)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(7861..8480)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(8635..9495)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(8635..9495)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(9537..9565)
                     /vntifkey="30"
                     /label=AmpR_promoter
     primer_bind     5452..5481
                     /vntifkey="28"
                     /label=pimp\my\pCDNA3-\F
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_signal     944..949
                     /vntifkey="87"
                     /label=Kozakovo\zaporedje
     misc_feature    5165..5167
                     /vntifkey="21"
                     /label=stop\kodon
     misc_feature    5096..5125
                     /vntifkey="21"
                     /label=gs10
     misc_feature    953..5095
                     /vntifkey="21"
                     /label=dCas9
     misc_feature    5126..5164
                     /vntifkey="21"
                     /label=DHFR[C174]
BASE COUNT     2456 a      2580 c      2562 g      2033 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgcaccacca 
      961 ccaccaccac gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg 
     1021 ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac 
     1081 cgaccggcac agcatcaaga agaacctgat cggcgccctg ctgttcgaca gcggagaaac 
     1141 agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg 
     1201 gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt 
     1261 ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat 
     1321 cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct 
     1381 gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct 
     1441 ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa 
     1501 cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga 
     1561 aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa 
     1621 gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt 
     1681 cggcaacctg attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct 
     1741 ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct 
     1801 gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga 
     1861 cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag 
     1921 cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct 
     1981 cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg 
     2041 ctacgccggc tacatcgatg gcggagccag ccaggaagag ttctacaagt tcatcaagcc 
     2101 catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct 
     2161 gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga 
     2221 gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga 
     2281 aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg 
     2341 aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt 
     2401 cgaggaagtg gtggacaagg gcgccagcgc ccagagcttc atcgagcgga tgaccaactt 
     2461 cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt 
     2521 caccgtgtac aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc 
     2581 cttcctgagc ggcgagcaga aaaaagccat cgtggacctg ctgttcaaga ccaaccggaa 
     2641 agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt 
     2701 ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct 
     2761 gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga 
     2821 tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac 
     2881 ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg 
     2941 ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac 
     3001 aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca 
     3061 cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga 
     3121 tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct 
     3181 gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa 
     3241 catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg 
     3301 cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga 
     3361 acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa 
     3421 tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt 
     3481 ggacgctatc gtgcctcaga gctttctgaa ggacgactcc atcgataaca aagtgctgac 
     3541 tcggagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa 
     3601 gatgaagaac tactggcgcc agctgctgaa tgccaagctg attacccaga ggaagttcga 
     3661 caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa 
     3721 gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg 
     3781 gatgaacact aagtacgacg agaacgacaa actgatccgg gaagtgaaag tgatcaccct 
     3841 gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat 
     3901 caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat 
     3961 caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt 
     4021 gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt 
     4081 ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg 
     4141 gaagcggcct ctgatcgaga caaacggcga aacaggcgag atcgtgtggg ataagggccg 
     4201 ggactttgcc accgtgcgga aagtgctgtc tatgccccaa gtgaatatcg tgaaaaagac 
     4261 cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgacaa 
     4321 gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac 
     4381 cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa 
     4441 gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc 
     4501 catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct 
     4561 gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg 
     4621 cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct 
     4681 ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt 
     4741 tgtggaacag cacaaacact acctggacga gatcatcgag cagatcagcg agttctccaa 
     4801 gagagtgatc ctggccgacg ctaatctgga caaggtgctg agcgcctaca acaagcacag 
     4861 agacaagcct atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct 
     4921 gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag 
     4981 caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac 
     5041 acggatcgac ctgtctcagc tgggaggctc tgatccaaaa aagaagagaa aggtaggcgg 
     5101 ctctggcggc ggctccggag gctctggtat taagtacaag tttgaggtct atgaaaagaa 
     5161 tgactaatct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag 
     5221 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 
     5281 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 
     5341 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 
     5401 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 
     5461 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 
     5521 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
     5581 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
     5641 ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
     5701 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
     5761 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
     5821 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct 
     5881 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 
     5941 gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca 
     6001 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 
     6061 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 
     6121 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 
     6181 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 
     6241 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 
     6301 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 
     6361 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 
     6421 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 
     6481 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt 
     6541 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 
     6601 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 
     6661 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 
     6721 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 
     6781 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 
     6841 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg 
     6901 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 
     6961 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 
     7021 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 
     7081 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct 
     7141 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac 
     7201 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 
     7261 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 
     7321 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 
     7381 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 
     7441 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
     7501 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 
     7561 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 
     7621 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 
     7681 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
     7741 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
     7801 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     7861 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     7921 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     7981 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     8041 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt 
     8101 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     8161 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     8221 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     8281 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 
     8341 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     8401 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     8461 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     8521 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     8581 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     8641 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     8701 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     8761 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     8821 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     8881 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     8941 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     9001 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     9061 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     9121 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     9181 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     9241 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     9301 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
     9361 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
     9421 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
     9481 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 
     9541 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 
     9601 cacatttccc cgaaaagtgc cacctgacgt c 
//
