LOCUS       pCMV-dCas9:nls:g        9757 bp    DNA     circular SYN 23-JUN-2022
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13206939|
COMMENT     VNTDBDATE|-14449652|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCMV-dCas9:nls:gs:ERb[C447]|
COMMENT     VNTAUTHORNAME|erik|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="pcDNA3"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(5310..5327)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(5311..5328)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(5347..5364)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      5350..5577
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      5640..5946
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(6060..6080)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(6066..6282)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        6078..6347
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      6246..6323
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    6308..6327
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             6462..7256
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            6465..7253
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      7433..7552
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    7521..7540
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(7596..7614)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(7613..7635)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(7649..7678)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(7987..8606)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(8761..9621)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(8761..9621)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(9663..9691)
                     /vntifkey="30"
                     /label=AmpR_promoter
     primer_bind     5578..5607
                     /vntifkey="28"
                     /label=pimp\my\pCDNA3-\F
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_signal     944..949
                     /vntifkey="87"
                     /label=Kozakovo\zaporedje
     misc_feature    5291..5293
                     /vntifkey="21"
                     /label=stop\kodon
     misc_feature    5096..5125
                     /vntifkey="21"
                     /label=gs10
     misc_feature    953..5095
                     /vntifkey="21"
                     /label=dCas9
     misc_feature    5126..5290
                     /vntifkey="21"
                     /label=ERb[C447]
BASE COUNT     2486 a      2616 c      2591 g      2064 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgcaccacca 
      961 ccaccaccac gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg 
     1021 ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac 
     1081 cgaccggcac agcatcaaga agaacctgat cggcgccctg ctgttcgaca gcggagaaac 
     1141 agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg 
     1201 gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt 
     1261 ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat 
     1321 cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct 
     1381 gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct 
     1441 ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa 
     1501 cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga 
     1561 aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa 
     1621 gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt 
     1681 cggcaacctg attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct 
     1741 ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct 
     1801 gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga 
     1861 cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag 
     1921 cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct 
     1981 cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg 
     2041 ctacgccggc tacatcgatg gcggagccag ccaggaagag ttctacaagt tcatcaagcc 
     2101 catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct 
     2161 gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga 
     2221 gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga 
     2281 aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg 
     2341 aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt 
     2401 cgaggaagtg gtggacaagg gcgccagcgc ccagagcttc atcgagcgga tgaccaactt 
     2461 cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt 
     2521 caccgtgtac aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc 
     2581 cttcctgagc ggcgagcaga aaaaagccat cgtggacctg ctgttcaaga ccaaccggaa 
     2641 agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt 
     2701 ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct 
     2761 gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga 
     2821 tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac 
     2881 ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg 
     2941 ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac 
     3001 aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca 
     3061 cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga 
     3121 tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct 
     3181 gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa 
     3241 catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg 
     3301 cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga 
     3361 acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa 
     3421 tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt 
     3481 ggacgctatc gtgcctcaga gctttctgaa ggacgactcc atcgataaca aagtgctgac 
     3541 tcggagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa 
     3601 gatgaagaac tactggcgcc agctgctgaa tgccaagctg attacccaga ggaagttcga 
     3661 caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa 
     3721 gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg 
     3781 gatgaacact aagtacgacg agaacgacaa actgatccgg gaagtgaaag tgatcaccct 
     3841 gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat 
     3901 caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat 
     3961 caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt 
     4021 gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt 
     4081 ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg 
     4141 gaagcggcct ctgatcgaga caaacggcga aacaggcgag atcgtgtggg ataagggccg 
     4201 ggactttgcc accgtgcgga aagtgctgtc tatgccccaa gtgaatatcg tgaaaaagac 
     4261 cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgacaa 
     4321 gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac 
     4381 cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa 
     4441 gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc 
     4501 catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct 
     4561 gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg 
     4621 cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct 
     4681 ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt 
     4741 tgtggaacag cacaaacact acctggacga gatcatcgag cagatcagcg agttctccaa 
     4801 gagagtgatc ctggccgacg ctaatctgga caaggtgctg agcgcctaca acaagcacag 
     4861 agacaagcct atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct 
     4921 gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag 
     4981 caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac 
     5041 acggatcgac ctgtctcagc tgggaggctc tgatccaaaa aagaagagaa aggtaggcgg 
     5101 ctctggcggc ggctccggag gctcttccag ccagcaacaa tctatgcgat tggcgaactt 
     5161 gcttatgctg ctcagtcacg taaggcatgc cagcaataaa gggatggaac atcttcttaa 
     5221 tatgaagtgt aagaatgtgg tgcccgttta cgatctcctg ctggaaatgc tcaacgcaca 
     5281 cgttctcagg taatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct 
     5341 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 
     5401 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 
     5461 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 
     5521 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 
     5581 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg 
     5641 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 
     5701 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 
     5761 gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta cggcacctcg 
     5821 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 
     5881 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 
     5941 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttggggattt 
     6001 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg 
     6061 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc 
     6121 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 
     6181 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 
     6241 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 
     6301 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt 
     6361 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca 
     6421 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 
     6481 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 
     6541 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 
     6601 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc 
     6661 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 
     6721 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 
     6781 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 
     6841 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 
     6901 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 
     6961 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga 
     7021 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 
     7081 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 
     7141 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 
     7201 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 
     7261 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 
     7321 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 
     7381 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat 
     7441 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 
     7501 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 
     7561 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 
     7621 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 
     7681 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 
     7741 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 
     7801 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 
     7861 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 
     7921 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 
     7981 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 
     8041 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 
     8101 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 
     8161 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag 
     8221 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 
     8281 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 
     8341 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 
     8401 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 
     8461 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 
     8521 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 
     8581 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 
     8641 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 
     8701 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 
     8761 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 
     8821 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 
     8881 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 
     8941 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 
     9001 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 
     9061 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 
     9121 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 
     9181 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 
     9241 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 
     9301 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 
     9361 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 
     9421 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 
     9481 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 
     9541 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 
     9601 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 
     9661 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 
     9721 tccgcgcaca tttccccgaa aagtgccacc tgacgtc 
//
