LOCUS       pCMV-dCas9:nls:g        9736 bp    DNA     circular SYN 23-JUN-2022
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14184962|
COMMENT     VNTDBDATE|-14449651|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCMV-dCas9:nls:gs:THRbC413|
COMMENT     VNTAUTHORNAME|erik|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="pcDNA3"
                     /mol_type="other DNA"
                     /vntifkey="98"
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(5289..5306)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(5290..5307)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(5326..5343)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      5329..5556
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      5619..5925
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(6039..6059)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(6045..6261)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        6057..6326
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      6225..6302
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    6287..6306
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             6441..7235
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            6444..7232
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      7412..7531
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    7500..7519
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(7575..7593)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(7592..7614)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(7628..7657)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(7966..8585)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(8740..9600)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(8740..9600)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(9642..9670)
                     /vntifkey="30"
                     /label=AmpR_promoter
     primer_bind     5557..5586
                     /vntifkey="28"
                     /label=pimp\my\pCDNA3-\F
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_signal     944..949
                     /vntifkey="87"
                     /label=Kozakovo\zaporedje
     misc_feature    5270..5272
                     /vntifkey="21"
                     /label=stop\kodon
     misc_feature    5096..5125
                     /vntifkey="21"
                     /label=gs10
     misc_feature    953..5095
                     /vntifkey="21"
                     /label=dCas9
     misc_feature    5126..5269
                     /vntifkey="21"
                     /label=THRbC413\His
BASE COUNT     2466 a      2625 c      2595 g      2050 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgcaccacca 
      961 ccaccaccac gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg 
     1021 ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac 
     1081 cgaccggcac agcatcaaga agaacctgat cggcgccctg ctgttcgaca gcggagaaac 
     1141 agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg 
     1201 gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt 
     1261 ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat 
     1321 cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct 
     1381 gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct 
     1441 ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa 
     1501 cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga 
     1561 aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa 
     1621 gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt 
     1681 cggcaacctg attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct 
     1741 ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct 
     1801 gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga 
     1861 cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag 
     1921 cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct 
     1981 cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg 
     2041 ctacgccggc tacatcgatg gcggagccag ccaggaagag ttctacaagt tcatcaagcc 
     2101 catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct 
     2161 gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga 
     2221 gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga 
     2281 aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg 
     2341 aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt 
     2401 cgaggaagtg gtggacaagg gcgccagcgc ccagagcttc atcgagcgga tgaccaactt 
     2461 cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt 
     2521 caccgtgtac aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc 
     2581 cttcctgagc ggcgagcaga aaaaagccat cgtggacctg ctgttcaaga ccaaccggaa 
     2641 agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt 
     2701 ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct 
     2761 gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga 
     2821 tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac 
     2881 ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg 
     2941 ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac 
     3001 aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca 
     3061 cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga 
     3121 tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct 
     3181 gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa 
     3241 catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg 
     3301 cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga 
     3361 acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa 
     3421 tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt 
     3481 ggacgctatc gtgcctcaga gctttctgaa ggacgactcc atcgataaca aagtgctgac 
     3541 tcggagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa 
     3601 gatgaagaac tactggcgcc agctgctgaa tgccaagctg attacccaga ggaagttcga 
     3661 caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa 
     3721 gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg 
     3781 gatgaacact aagtacgacg agaacgacaa actgatccgg gaagtgaaag tgatcaccct 
     3841 gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat 
     3901 caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat 
     3961 caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt 
     4021 gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt 
     4081 ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg 
     4141 gaagcggcct ctgatcgaga caaacggcga aacaggcgag atcgtgtggg ataagggccg 
     4201 ggactttgcc accgtgcgga aagtgctgtc tatgccccaa gtgaatatcg tgaaaaagac 
     4261 cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgacaa 
     4321 gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac 
     4381 cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa 
     4441 gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc 
     4501 catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct 
     4561 gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg 
     4621 cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct 
     4681 ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt 
     4741 tgtggaacag cacaaacact acctggacga gatcatcgag cagatcagcg agttctccaa 
     4801 gagagtgatc ctggccgacg ctaatctgga caaggtgctg agcgcctaca acaagcacag 
     4861 agacaagcct atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct 
     4921 gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag 
     4981 caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac 
     5041 acggatcgac ctgtctcagc tgggaggctc tgatccaaaa aagaagagaa aggtaggcgg 
     5101 ctctggcggc ggctccggag gctctcacgt gacccacttc tggcccaagc tgctgatgaa 
     5161 ggtgaccgac ctgaggatga tcggcgcctg ccacgccagc aggttcctgc acatgaaggt 
     5221 ggagtgcccc accgagctgt tcccccccct gttcctggag gtgttcgagt aatctagagg 
     5281 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt 
     5341 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 
     5401 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 
     5461 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 
     5521 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct 
     5581 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 
     5641 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 
     5701 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca 
     5761 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 
     5821 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 
     5881 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 
     5941 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg 
     6001 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg 
     6061 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt 
     6121 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 
     6181 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 
     6241 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 
     6301 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 
     6361 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga 
     6421 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 
     6481 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 
     6541 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 
     6601 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg 
     6661 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 
     6721 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 
     6781 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 
     6841 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 
     6901 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 
     6961 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 
     7021 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 
     7081 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 
     7141 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 
     7201 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac 
     7261 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 
     7321 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 
     7381 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa 
     7441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     7501 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta 
     7561 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 
     7621 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 
     7681 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 
     7741 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 
     7801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
     7861 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
     7921 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     7981 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     8041 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     8101 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     8161 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     8221 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     8281 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     8341 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     8401 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 
     8461 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     8521 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     8581 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     8641 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     8701 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     8761 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     8821 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     8881 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     8941 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     9001 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     9061 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     9121 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     9181 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     9241 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     9301 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     9361 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     9421 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     9481 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     9541 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     9601 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     9661 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     9721 agtgccacct gacgtc 
//
