LOCUS       pCMV-ERb[N446]:g        7639 bp    DNA     circular SYN  9-FEB-2020
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13204246|
COMMENT     VNTDBDATE|-13204246|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCMV-ERb[N446]:gs10:NLS:VPR|
COMMENT     VNTAUTHORNAME|erik|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(3192..3209)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(3193..3210)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(3229..3246)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      3232..3459
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      3522..3828
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(3942..3962)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(3948..4164)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        3960..4229
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      4128..4205
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    4190..4209
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             4344..5138
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            4347..5135
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      5315..5434
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    5403..5422
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(5478..5496)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(5495..5517)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(5531..5560)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(5869..6488)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(6643..7503)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(6643..7503)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(7545..7573)
                     /vntifkey="30"
                     /label=AmpR_promoter
     primer_bind     3460..3489
                     /vntifkey="28"
                     /label=pimp\my\pCDNA3-\F
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_signal     944..949
                     /vntifkey="87"
                     /label=Kozakovo\zaporedje
     misc_signal     1544..1567
                     /vntifkey="87"
                     /label=NLS
     misc_feature    1568..3154
                     /vntifkey="21"
                     /label=VPR
     misc_feature    1514..1543
                     /vntifkey="21"
                     /label=gs10
     misc_feature    3155..3175
                     /vntifkey="21"
                     /label=au1
     misc_feature    956..1513
                     /vntifkey="21"
                     /label=ERb[N446]
BASE COUNT     1737 a      2102 c      1969 g      1831 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgcacgacgc 
      961 tctgtcacct gaacagctcg ttttgacgct tttggaagct gaaccaccac acgttctgat 
     1021 cagccgaccc tctgctccat tcacagaggc cagtatgatg atgagcctca ctaagttggc 
     1081 cgataaagaa cttgtccata tgatttcctg ggcgaagaaa atacccggat tcgtagagct 
     1141 ttccctcttc gatcaggtac ggctgctcga gagttgctgg atggaggttc tgatgatggg 
     1201 gctgatgtgg agatccatag atcaccccgg taaacttatc tttgcgccag accttgtact 
     1261 cgaccgggac gaaggaaaat gcgtagaagg gatcctggaa atttttgata tgctgctcgc 
     1321 taccaccagt cggttcaggg agctgaaatt gcagcacaag gaatatcttt gcgtgaaagc 
     1381 gatgatactt ctcaattcaa gtatgtaccc cctcgtgaca gcaacccagg acgcagatag 
     1441 ctccagaaaa cttgctcatc ttcttaatgc ggtcaccgac gcattggtct gggttattgc 
     1501 aaagtctggt ataggcggct ctggcggcgg ctccggaggc tctgatccaa aaaagaagag 
     1561 aaaggtagag gccagcggtt cgggacgggc tgacgcattg gacgattttg atctggatat 
     1621 gctgggaagt gacgccctcg atgattttga ccttgacatg cttggttcgg atgcccttga 
     1681 tgactttgac ctcgacatgc tcggcagtga cgcccttgat gatttcgacc tggacatgct 
     1741 gattaacact agtggcggct ctggcggcgg ctccggaggc tctagccagt acctgcccga 
     1801 caccgacgac cggcaccgga tcgaggaaaa gcggaagcgg acctacgaga cattcaagag 
     1861 catcatgaag aagtccccct tcagcggccc caccgaccct agacctccac ctagaagaat 
     1921 cgccgtgccc agcagatcca gcgccagcgt gccaaaacct gccccccagc cttacccctt 
     1981 caccagcagc ctgagcacca tcaactacga cgagttccct accatggtgt tccccagcgg 
     2041 ccagatctct caggcctctg ctctggctcc agcccctcct caggtgctgc ctcaggctcc 
     2101 tgctcctgca ccagctccag ccatggtgtc tgcactggct caggcaccag cacccgtgcc 
     2161 tgtgctggct cctggacctc cacaggctgt ggctccacca gcccctaaac ctacacaggc 
     2221 cggcgagggc acactgtctg aagctctgct gcagctgcag ttcgacgacg aggatctggg 
     2281 agccctgctg ggaaacagca ccgatcctgc cgtgttcacc gacctggcca gcgtggacaa 
     2341 cagcgagttc cagcagctgc tgaaccaggg catccctgtg gcccctcaca ccaccgagcc 
     2401 catgctgatg gaataccccg aggccatcac ccggctcgtg acaggcgctc agaggcctcc 
     2461 tgatccagct cctgcccctc tgggagcacc aggcctgcct aatggactgc tgtctggcga 
     2521 cgaggacttc agctctatcg ccgatatgga tttctcagcc ttgctgggct ctggcagcgg 
     2581 cagccgggat tccagggaag ggatgttttt gccgaagcct gaggccggct ccgctattag 
     2641 tgacgtgttt gagggccgcg aggtgtgcca gccaaaacga atccggccat ttcatcctcc 
     2701 aggaagtcca tgggccaacc gcccactccc cgccagcctc gcaccaacac caaccggtcc 
     2761 agtacatgag ccagtcgggt cactgacccc ggcaccagtc cctcagccac tggacccagc 
     2821 gcccgcagtg actcccgagg ccagtcacct gttggaggac cccgatgaag agacgagcca 
     2881 ggctgtcaaa gcccttcggg agatggccga tactgtgatt ccccagaagg aagaggctgc 
     2941 aatctgtggc caaatggacc tttcccatcc gcccccaagg ggccatctgg atgagctgac 
     3001 aaccacactt gagtccatga ccgaggatct gaacctggac tcacccctga ccccggaatt 
     3061 gaacgagatt ctggatacct tcctgaacga cgagtgcctc ttgcatgcca tgcatatcag 
     3121 cacaggactg tccatcttcg acacatctct gtttgacaca tacaggtata tctaatctag 
     3181 agggccctat tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc 
     3241 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 
     3301 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 
     3361 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 
     3421 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca 
     3481 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg 
     3541 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 
     3601 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 
     3661 gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 
     3721 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 
     3781 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 
     3841 tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa 
     3901 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg 
     3961 gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt 
     4021 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 
     4081 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 
     4141 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 
     4201 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 
     4261 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag 
     4321 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 
     4381 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 
     4441 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 
     4501 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga 
     4561 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 
     4621 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 
     4681 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 
     4741 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 
     4801 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 
     4861 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 
     4921 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 
     4981 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 
     5041 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 
     5101 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 
     5161 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 
     5221 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 
     5281 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 
     5341 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 
     5401 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 
     5461 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     5521 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     5581 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     5641 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     5701 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     5761 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     5821 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     5881 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     5941 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     6001 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     6061 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     6121 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     6181 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     6241 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     6301 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 
     6361 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     6421 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     6481 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     6541 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     6601 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     6661 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     6721 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     6781 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     6841 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     6901 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     6961 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     7021 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     7081 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     7141 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     7201 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
     7261 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     7321 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     7381 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     7441 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     7501 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     7561 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
     7621 aaaagtgcca cctgacgtc 
//
