LOCUS       pCMV-AncGR2N175:        7603 bp    DNA     circular SYN 31-AUG-2019
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12959450|
COMMENT     VNTDBDATE|-12970973|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCMV-AncGR2N175:gs10:NLS:VPR|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(3156..3173)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(3157..3174)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(3193..3210)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      3196..3423
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      3486..3792
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(3906..3926)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(3912..4128)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        3924..4193
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      4092..4169
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    4154..4173
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             4308..5102
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            4311..5099
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      5279..5398
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    5367..5386
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(5442..5460)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(5459..5481)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(5495..5524)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(5833..6452)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(6607..7467)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(6607..7467)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(7509..7537)
                     /vntifkey="30"
                     /label=AmpR_promoter
     primer_bind     3424..3453
                     /vntifkey="28"
                     /label=pimp\my\pCDNA3-\F
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_signal     944..949
                     /vntifkey="87"
                     /label=Kozakovo\zaporedje
     misc_feature    950..952
                     /vntifkey="21"
                     /label=start\kodon
     misc_signal     1508..1531
                     /vntifkey="87"
                     /label=NLS
     misc_feature    1532..3118
                     /vntifkey="21"
                     /label=VPR
     misc_feature    953..1477
                     /vntifkey="21"
                     /label=AncGR2N175
     misc_feature    1478..1507
                     /vntifkey="21"
                     /label=gs10
     misc_feature    3119..3139
                     /vntifkey="21"
                     /label=au1
BASE COUNT     1714 a      2112 c      2008 g      1769 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgctgatcag 
      961 cctgctggag gtgatcgagc ccgaggtgct gtacagcggc tacgacagca ccctgcccga 
     1021 caccagcacc aggctgatga gcaccctgaa caggctgggc ggcaggcagg tggtgagcgc 
     1081 cgtgaagtgg gccaaggccc tgcccggctt caggaacctg cacctggacg accagatgac 
     1141 cctgctgcag tacagctgga tgagcctgat ggccttcagc ctgggctgga ggagctacaa 
     1201 gcagagcaac ggcaacatgc tgtgcttcgc ccccgacctg gtgatcaacg aggagaggat 
     1261 gcagctgccc tacatgtacg accagtgcca gcagatgctg aagatcagca gcgagttcgt 
     1321 gaggctgcag gtgagctacg acgagtacct gtgcatgaag gtgctgctgc tgctgagcac 
     1381 cgtgcccaag gacggcctga agagccaggc cgtgttcgac gagatcagga tgacctacat 
     1441 caaggagctg ggcaaggcca tcgtgaagag ggagggcggc ggctctggcg gcggctccgg 
     1501 aggctctgat ccaaaaaaga agagaaaggt agaggccagc ggttcgggac gggctgacgc 
     1561 attggacgat tttgatctgg atatgctggg aagtgacgcc ctcgatgatt ttgaccttga 
     1621 catgcttggt tcggatgccc ttgatgactt tgacctcgac atgctcggca gtgacgccct 
     1681 tgatgatttc gacctggaca tgctgattaa cactagtggc ggctctggcg gcggctccgg 
     1741 aggctctagc cagtacctgc ccgacaccga cgaccggcac cggatcgagg aaaagcggaa 
     1801 gcggacctac gagacattca agagcatcat gaagaagtcc cccttcagcg gccccaccga 
     1861 ccctagacct ccacctagaa gaatcgccgt gcccagcaga tccagcgcca gcgtgccaaa 
     1921 acctgccccc cagccttacc ccttcaccag cagcctgagc accatcaact acgacgagtt 
     1981 ccctaccatg gtgttcccca gcggccagat ctctcaggcc tctgctctgg ctccagcccc 
     2041 tcctcaggtg ctgcctcagg ctcctgctcc tgcaccagct ccagccatgg tgtctgcact 
     2101 ggctcaggca ccagcacccg tgcctgtgct ggctcctgga cctccacagg ctgtggctcc 
     2161 accagcccct aaacctacac aggccggcga gggcacactg tctgaagctc tgctgcagct 
     2221 gcagttcgac gacgaggatc tgggagccct gctgggaaac agcaccgatc ctgccgtgtt 
     2281 caccgacctg gccagcgtgg acaacagcga gttccagcag ctgctgaacc agggcatccc 
     2341 tgtggcccct cacaccaccg agcccatgct gatggaatac cccgaggcca tcacccggct 
     2401 cgtgacaggc gctcagaggc ctcctgatcc agctcctgcc cctctgggag caccaggcct 
     2461 gcctaatgga ctgctgtctg gcgacgagga cttcagctct atcgccgata tggatttctc 
     2521 agccttgctg ggctctggca gcggcagccg ggattccagg gaagggatgt ttttgccgaa 
     2581 gcctgaggcc ggctccgcta ttagtgacgt gtttgagggc cgcgaggtgt gccagccaaa 
     2641 acgaatccgg ccatttcatc ctccaggaag tccatgggcc aaccgcccac tccccgccag 
     2701 cctcgcacca acaccaaccg gtccagtaca tgagccagtc gggtcactga ccccggcacc 
     2761 agtccctcag ccactggacc cagcgcccgc agtgactccc gaggccagtc acctgttgga 
     2821 ggaccccgat gaagagacga gccaggctgt caaagccctt cgggagatgg ccgatactgt 
     2881 gattccccag aaggaagagg ctgcaatctg tggccaaatg gacctttccc atccgccccc 
     2941 aaggggccat ctggatgagc tgacaaccac acttgagtcc atgaccgagg atctgaacct 
     3001 ggactcaccc ctgaccccgg aattgaacga gattctggat accttcctga acgacgagtg 
     3061 cctcttgcat gccatgcata tcagcacagg actgtccatc ttcgacacat ctctgtttga 
     3121 cacatacagg tatatctaat ctagagggcc ctattctata gtgtcaccta aatgctagag 
     3181 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 
     3241 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 
     3301 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 
     3361 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 
     3421 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta 
     3481 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 
     3541 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 
     3601 ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc 
     3661 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 
     3721 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 
     3781 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg 
     3841 ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 
     3901 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa 
     3961 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 
     4021 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 
     4081 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 
     4141 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga 
     4201 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta 
     4261 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag 
     4321 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 
     4381 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 
     4441 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 
     4501 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 
     4561 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 
     4621 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 
     4681 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 
     4741 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 
     4801 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg 
     4861 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 
     4921 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 
     4981 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 
     5041 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 
     5101 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 
     5161 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 
     5221 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 
     5281 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     5341 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 
     5401 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 
     5461 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     5521 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     5581 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
     5641 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     5701 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     5761 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 
     5821 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 
     5881 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 
     5941 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 
     6001 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta 
     6061 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 
     6121 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 
     6181 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 
     6241 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 
     6301 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 
     6361 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 
     6421 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 
     6481 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 
     6541 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 
     6601 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 
     6661 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 
     6721 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 
     6781 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 
     6841 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 
     6901 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 
     6961 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 
     7021 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 
     7081 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 
     7141 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 
     7201 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 
     7261 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 
     7321 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 
     7381 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 
     7441 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 
     7501 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 
     7561 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc 
//
