LOCUS       pCMV-THRbN412:gs        7717 bp    DNA     circular SYN  8-JUL-2020
DEFINITION  pcDNA3.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13420195|
COMMENT     VNTDBDATE|-13420195|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCMV-THRbN412:gs10:NLS:VPR (new)|
COMMENT     VNTAUTHORNAME|Mega lab|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
FEATURES             Location/Qualifiers
     misc_feature    315..602
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        236..852
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    769..789
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        863..881
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    complement(3270..3287)
                     /vntifkey="21"
                     /label=Sp6_primer
     promoter        complement(3271..3288)
                     /vntifkey="30"
                     /label=Sp6_promoter
     misc_feature    complement(3307..3324)
                     /vntifkey="21"
                     /label=BGH_rev_primer
     terminator      3310..3537
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     rep_origin      3600..3906
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    complement(4020..4040)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(4026..4242)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        4038..4307
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      4206..4283
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    4268..4287
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             4422..5216
                     /gene="Neomycin"
                     /vntifkey="4"
                     /label=Neomycin
                     /note="ORF frame 3"
     gene            4425..5213
                     /gene="Neomycin"
                     /vntifkey="60"
     terminator      5393..5512
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    5481..5500
                     /vntifkey="21"
                     /label=EBV_rev_primer
     misc_feature    complement(5556..5574)
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(5573..5595)
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        complement(5609..5638)
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      complement(5947..6566)
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             complement(6721..7581)
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            complement(6721..7581)
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        complement(7623..7651)
                     /vntifkey="30"
                     /label=AmpR_promoter
     primer_bind     3538..3567
                     /vntifkey="28"
                     /label=pimp\my\pCDNA3-\F
     misc_feature    206..235
                     /vntifkey="21"
                     /label=pimp\my\pCDNA3\-r
     misc_signal     944..949
                     /vntifkey="87"
                     /label=Kozakovo\zaporedje
     misc_signal     1622..1645
                     /vntifkey="87"
                     /label=NLS
     misc_feature    1646..3232
                     /vntifkey="21"
                     /label=VPR
     misc_feature    1592..1621
                     /vntifkey="21"
                     /label=gs10
     misc_feature    956..1591
                     /vntifkey="21"
                     /label=THRbN412
     misc_feature    3233..3253
                     /vntifkey="21"
                     /label=au1
BASE COUNT     1738 a      2167 c      2039 g      1773 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tgcacatgga 
      961 ggagctgcag aagagcatcg gccacaagcc cgagcccacc gacgaggagt gggagctgat 
     1021 caagaccgtg accgaggccc acgtggccac caacgcccag ggcagccact ggaagcagaa 
     1081 gaggaagttc ctgcccgagg acatcggcca ggcccccatc gtgaacgccc ccgagggcgg 
     1141 caaggtggac ctggaggcct tcagccactt caccaagatc atcacccccg ccatcaccag 
     1201 ggtggtggac ttcgccaaga agctgcccat gttctgcgag ctgccctgcg aggaccagat 
     1261 catcctgctg aagggctgct gcatggagat catgagcctg agggccgccg tgaggtacga 
     1321 ccccgagagc gagaccctga ccctgaacgg cgagatggcc gtgaccaggg gccagctgaa 
     1381 gaacggcggc ctgggcgtgg tgagcgacgc catcttcgac ctgggcatga gcctgagcag 
     1441 cttcaacctg gacgacaccg aggtggccct gctgcaggcc gtgctgctga tgagcagcga 
     1501 caggcccggc ctggcctgcg tggagaggat cgagaagtac caggacagct tcctgctggc 
     1561 cttcgagcac tacatcaact acaggaagca cggcggctct ggcggcggct ccggaggctc 
     1621 tgatccaaaa aagaagagaa aggtagaggc cagcggttcg ggacgggctg acgcattgga 
     1681 cgattttgat ctggatatgc tgggaagtga cgccctcgat gattttgacc ttgacatgct 
     1741 tggttcggat gcccttgatg actttgacct cgacatgctc ggcagtgacg cccttgatga 
     1801 tttcgacctg gacatgctga ttaacactag tggcggctct ggcggcggct ccggaggctc 
     1861 tagccagtac ctgcccgaca ccgacgaccg gcaccggatc gaggaaaagc ggaagcggac 
     1921 ctacgagaca ttcaagagca tcatgaagaa gtcccccttc agcggcccca ccgaccctag 
     1981 acctccacct agaagaatcg ccgtgcccag cagatccagc gccagcgtgc caaaacctgc 
     2041 cccccagcct taccccttca ccagcagcct gagcaccatc aactacgacg agttccctac 
     2101 catggtgttc cccagcggcc agatctctca ggcctctgct ctggctccag cccctcctca 
     2161 ggtgctgcct caggctcctg ctcctgcacc agctccagcc atggtgtctg cactggctca 
     2221 ggcaccagca cccgtgcctg tgctggctcc tggacctcca caggctgtgg ctccaccagc 
     2281 ccctaaacct acacaggccg gcgagggcac actgtctgaa gctctgctgc agctgcagtt 
     2341 cgacgacgag gatctgggag ccctgctggg aaacagcacc gatcctgccg tgttcaccga 
     2401 cctggccagc gtggacaaca gcgagttcca gcagctgctg aaccagggca tccctgtggc 
     2461 ccctcacacc accgagccca tgctgatgga ataccccgag gccatcaccc ggctcgtgac 
     2521 aggcgctcag aggcctcctg atccagctcc tgcccctctg ggagcaccag gcctgcctaa 
     2581 tggactgctg tctggcgacg aggacttcag ctctatcgcc gatatggatt tctcagcctt 
     2641 gctgggctct ggcagcggca gccgggattc cagggaaggg atgtttttgc cgaagcctga 
     2701 ggccggctcc gctattagtg acgtgtttga gggccgcgag gtgtgccagc caaaacgaat 
     2761 ccggccattt catcctccag gaagtccatg ggccaaccgc ccactccccg ccagcctcgc 
     2821 accaacacca accggtccag tacatgagcc agtcgggtca ctgaccccgg caccagtccc 
     2881 tcagccactg gacccagcgc ccgcagtgac tcccgaggcc agtcacctgt tggaggaccc 
     2941 cgatgaagag acgagccagg ctgtcaaagc ccttcgggag atggccgata ctgtgattcc 
     3001 ccagaaggaa gaggctgcaa tctgtggcca aatggacctt tcccatccgc ccccaagggg 
     3061 ccatctggat gagctgacaa ccacacttga gtccatgacc gaggatctga acctggactc 
     3121 acccctgacc ccggaattga acgagattct ggataccttc ctgaacgacg agtgcctctt 
     3181 gcatgccatg catatcagca caggactgtc catcttcgac acatctctgt ttgacacata 
     3241 caggtatatc taatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct 
     3301 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 
     3361 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 
     3421 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 
     3481 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 
     3541 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg 
     3601 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 
     3661 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 
     3721 gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta cggcacctcg 
     3781 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 
     3841 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 
     3901 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttggggattt 
     3961 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg 
     4021 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc 
     4081 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 
     4141 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 
     4201 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 
     4261 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt 
     4321 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca 
     4381 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 
     4441 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 
     4501 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 
     4561 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc 
     4621 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 
     4681 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 
     4741 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 
     4801 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 
     4861 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 
     4921 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga 
     4981 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 
     5041 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 
     5101 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 
     5161 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 
     5221 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 
     5281 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 
     5341 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat 
     5401 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 
     5461 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 
     5521 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 
     5581 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 
     5641 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 
     5701 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 
     5761 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 
     5821 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 
     5881 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 
     5941 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 
     6001 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 
     6061 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 
     6121 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag 
     6181 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 
     6241 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 
     6301 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 
     6361 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 
     6421 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 
     6481 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 
     6541 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 
     6601 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 
     6661 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 
     6721 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 
     6781 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 
     6841 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 
     6901 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 
     6961 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 
     7021 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 
     7081 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 
     7141 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 
     7201 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 
     7261 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 
     7321 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 
     7381 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 
     7441 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 
     7501 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 
     7561 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 
     7621 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 
     7681 tccgcgcaca tttccccgaa aagtgccacc tgacgtc 
//
