LOCUS       Exported                1135 bp DNA     linear   SYN 27-JUN-2022
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 27, 2022 from SnapGene Viewer 6.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gap             2..12
                     /estimated_length=11
     CDS             22..528
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="FTLEXFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIV
                     LSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTP
                     NMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRL
                     CERILA"
     CDS             544..567
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     gap             1084..1094
                     /estimated_length=11
ORIGIN
        1 cnnnnnnnnn nnagtnngnn cttcacactc gaaganttcg ttggggactg gcgacagaca
       61 gccggctaca acctggacca agtccttgaa cagggaggtg tgtccagttt gtttcagaat
      121 ctcggggtgt ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa tgggctgaag
      181 atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc
      241 gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc actttaaggt gatcctgcac
      301 tatggcacac tggtaatcga cggggttacg ccgaacatga tcgactattt cggacggccg
      361 tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg taacagggac cctgtggaac
      421 ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg gctccctgct gttccgagta
      481 accatcaacg gagtgaccgg ctggcggctg tgcgaacgca ttctggcgac gcgtggaggt
      541 ggccatcacc atcatcacca tcaccacgga taactcgagc aaagcccgcc gaaaggcggg
      601 cttttctgta caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
      661 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgggct
      721 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      781 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      841 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      901 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttnngntt
      961 ncngatttag tgctttacgn cacctcnacc ccaaaaaaaa nttngattnn nggngatgnn
     1021 ntcacgtann ggnnnnncnn nnnatagang nntnncncnn ttgacnnnnn nantnnnnnn
     1081 nttnnnnnnn nnnntnnnnt nnnnannnnn ncaacnnctc annnccnnna annnn
//
