LOCUS       Exported                1153 bp DNA     linear   SYN 27-JUN-2022
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 27, 2022 from SnapGene Viewer 6.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(60..572)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     RBS             591..613
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(622..640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gap             1086..1095
                     /estimated_length=10
     gap             1117..1127
                     /estimated_length=11
ORIGIN
        1 nnnnnnnttn gctcnnntan ngtggtgagn tgatgatggt gatggccacc tccacgcgtc
       61 gccagaatgc gttcgcacag ccgccagccg gtcactccgt tgatggttac tcggaacagc
      121 agggagccgt cggggttgat caggcgctcg tcgataattt tgttgccgtt ccacagggtc
      181 cctgttacag tgatcttttt gccgtcgaac acggcgatgc cttcatacgg ccgtccgaaa
      241 tagtcgatca tgttcggcgt aaccccgtcg attaccagtg tgccatagtg caggatcacc
      301 ttaaagtgat gatcatccac agggtacacc accttaaaaa ttttttcgat ctggcccatt
      361 tggtcgccgc tcagaccttc atacgggatg atgacatgga tgtcgatctt cagcccattt
      421 tcaccgctca ggacaatcct ttggatcgga gttacggaca ccccgagatt ctgaaacaaa
      481 ctggacacac ctccctgttc aaggacttgg tccaggttgt agccggctgt ctgtcgccag
      541 tccccaacga aatcttcgag tgtgaagacc atactagtca taccggtata tctccttctt
      601 aaagttaaac aaaattattt ccctatagtg agtcgtatta gaattagatc tgtcgagcca
      661 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      721 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      781 gaaacccgac aggactataa agataccagg cgtttccccc ctggaagctc cctcgtgcgc
      841 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      901 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      961 aagctggggc tgtgtgcacg aaaccccccc gtttcagccc gaccgctgcg ccnttatncn
     1021 ggtaantatc gtcttngant nccancccgg gtaaganncn acttntcnnn ntnnnnnagc
     1081 cnactnnnnn nnnnntnanc canannnnnn nnntgtnnnn nnnnnnntta cnnnnnnntc
     1141 tttnnnannn nnn
//
