LOCUS       Exported                1156 bp DNA     linear   SYN 27-JUN-2022
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 27, 2022 from SnapGene Viewer 6.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gap             1..15
                     /estimated_length=15
     promoter        242..260
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             269..291
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     gap             1115..1124
                     /estimated_length=10
     gap             1137..1146
                     /estimated_length=10
ORIGIN
        1 nnnnnnnnnn nnnnncnnna gcggcagggt cggaacagga gagcgcacga gggagcttcc
       61 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      121 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      181 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atggctcgac agatctaatt
      241 ctaatacgac tcactatagg gaaataattt tgtttaactt taagaaggag atataccggt
      301 atgactagta tgcgtatcaa catctccctg tcgtcgttat tcgaacggtt aagtaaactg
      361 agctctcgtt caattgcgat tacctgtgga gttgtcctcg cctctgctat tgcttttccc
      421 atcatacgtc gggattatca gacgttcctg gaggttggtc ctagctatgc cccgcaaaac
      481 tttcgcggct acattatcgt ttgcgtactt agcttgttcc gccaagagca gaaaggcctg
      541 gcgatctacg accggctccc agagaagcgc cgctggctgg cggatctgcc atttcgtgaa
      601 ggtaccagac cgagtattac ctcacacatt atccagcgcc agcgcactca acttgtggat
      661 caggaatttg ccacacgtga actgattgac aaggttattc cgagggtgca agcacgacat
      721 accgataaaa catttttgag cacgagcaag tttgaatttc atgcgaaagc cattttcctg
      781 ttgccatcga ttccaatcaa cgacccgctg aacataccta gtcatgatac ggtgcgtcga
      841 acgaaacgcg aaattgccca catgcatgac tatcacgatt gcacccttca cctagctctg
      901 gcggcacagg gatgggaaaa gaagtcctga aaaaaannct gggggccagc gtcatccgct
      961 ggncgggnnn gggtgtgccc tgnncgccga ctgaatnnnc tttcttatac nccacccccg
     1021 taatgannnn nncgcgncgt ggnnanaaan ngatgnnnnn catcccatcn ggntatntng
     1081 annnngantc cgggcnnnna aanncgnncg anaannnnnn nnnngnngnn tcnnngnnnn
     1141 nnnnnncant nggnan
//
