LOCUS       Exported                1153 bp DNA     linear   SYN 27-JUN-2022
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 27, 2022 from SnapGene Viewer 6.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gap             1..13
                     /estimated_length=13
     promoter        240..258
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             267..289
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     gap             1120..1130
                     /estimated_length=11
     gap             1143..1153
                     /estimated_length=11
ORIGIN
        1 nnnnnnnnnn nnntcggtag cggcagggtc ggacaggana gcgcacgagg gagcttccag
       61 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      121 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      181 ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag atctaattct
      241 aatacgactc actataggga aataattttg tttaacttta agaaggagat ataccggtat
      301 gactagtatg gtgagcggct ggcggctgtt caagaagatt agcggaggtg gtggatcggg
      361 cggaggggga tcaatggagc ttttcactgg cgttgttccc atcctggtcg agctggacgg
      421 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
      481 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      541 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
      601 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
      661 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      721 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      781 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
      841 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
      901 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      961 cctgagcacc cagtccgccc tgagcaaaga ccccaacgan aagcgcnatc acatggncct
     1021 gctggnnttc gtgnncgccn ccngggnnca cnnnnnnngt gnnccatnca ccatcatcnn
     1081 cnntcnnnnn cggnnnactc nnngncannn nnnnncnnan nnnnnnnnnn cnnnnnncaa
     1141 ncnnnnnnnn nnn
//
