LOCUS       pML225_pGP8GF-ZmAFB2/3_ 7549 bp ds-DNA     circular     10-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    60..241
                     /label="ADH1 Terminator"
     primer_bind     complement(212..236)
                     /label="pGILDA-R"
     primer_bind     242..280
                     /label="oNL1872 GPDf"
     misc_feature    242..896
                     /label="Actual GPD Promoter"
     misc_feature    785..808
                     /label="GPDPro_F_primer (2002)"
     misc_feature    897..913
                     /label="pBluescriptSK_primer"
     misc_feature    921..924
                     /label="attR1"
     misc_feature    926..2644
                     /label="ZmAFB2/3 a"
     CDS             926..2648
                     /label="Translation 926-2648"
     CDS             2648..2740
                     /label="Translation 2648-2740"
     protein_bind    2657..2722
                     /label="3xFLAG"
     misc_binding    2723..2740
                     /label="6xHis"
     misc_feature    2788..2805
                     /label="attR2"
     misc_feature    complement(2788..2805)
                     /label="attR1"
     misc_feature    2806..2888
                     /label="attR2"
     misc_feature    complement(2806..2895)
                     /label="attR1"
     primer_bind     complement(2971..3000)
                     /label="oNL1913 TCYC1 front Rev"
     terminator      2971..3210
                     /label="CYC1_terminator"
     misc_feature    complement(2980..2998)
                     /label="pYESTrp_rev_primer"
     misc_feature    complement(2980..2998)
                     /label="CYC1_primer"
     primer_bind     complement(3187..3210)
                     /label="oNL1475 TCYC1r"
     misc_feature    3211..4211
                     /label="HIS3 3'UTR + DED1 homology"
     misc_feature    4212..4219
                     /label="PmeI site"
     misc_feature    complement(4220..4238)
                     /label="M13_reverse_primer"
     primer_bind     complement(4224..4240)
                     /label="M13R"
     misc_feature    complement(4237..4259)
                     /label="M13_pUC_rev_primer"
     misc_binding    complement(4246..4268)
                     /label="LacO"
     rep_origin      complement(4608..5290)
                     /label="ColE1 origin"
     rep_origin      complement(4611..5230)
                     /label="pBR322_origin"
     gene            complement(5385..5528)
                     /label="Ampicillin"
     primer_bind     complement(5504..5542)
                     /label="j5_0066_(pGP8G1)_(CtoT)_reverse"
     primer_bind     5517..5547
                     /label="j5_0067_(pGP8G2-start)_forward"
     misc_feature    5529..5529
                     /label="p"
     CDS             complement(5530..6245)
                     /label="Ampicillin"
     promoter        complement(6246..6344)
                     /label="bla promoter"
     promoter        complement(6287..6315)
                     /label="AmpR_promoter"
     misc_feature    complement(6464..6486)
                     /label="pGEX_3_primer"
     primer_bind     complement(6464..6486)
                     /label="3GEX"
     misc_feature    6620..6625
                     /label="unused HIS3 5' UTR homology"
     misc_feature    6626..6633
                     /label="PmeI site"
     misc_feature    6634..6945
                     /label="HIS3 5' UTR"
     misc_feature    6946..59
                     /label="HIS3"
ORIGIN
        1 aagccacctc gcccaatggt accaacgatg ttccctccac caaaggtgtt cttatgtagg
       61 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat
      121 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt
      181 tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct
      241 cagtttatca ttatcaatac tcgccatttc aaagaatacg taaataatta atagtagtga
      301 ttttcctaac tttatttagt caaaaaatta gccttttaat tctgctgtaa cccgtacatg
      361 cccaaaatag ggggcgggtt acacagaata tataacatcg taggtgtctg ggtgaacagt
      421 ttattcctgg catccactaa atataatgga gcccgctttt taagctggca tccagaaaaa
      481 aaaagaatcc cagcaccaaa atattgtttt cttcaccaac catcagttca taggtccatt
      541 ctcttagcgc aactacagag aacaggggca caaacaggca aaaaacgggc acaacctcaa
      601 tggagtgatg caacctgcct ggagtaaatg atgacacaag gcaattgacc cacgcatgta
      661 tctatctcat tttcttacac cttctattac cttctgctct ctctgatttg gaaaaagctg
      721 aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct acttgactaa taagtatata
      781 aagacggtag gtattgattg taattctgta aatctatttc ttaaacttct taaattctac
      841 ttttatagtt agtctttttt ttagttttaa aacaccagaa cttagtttcg acggattcta
      901 gaactagtgg atcccccatc acaaaatgGC GTACTTTCCA GACGAAGTAG TTGGGTACAT
      961 TTTAGGGTAC GTAACATCTC ACCAAGATAG AAATGCGGTT AGCTTGGTTT GCAGGGCATG
     1021 GTACGACATT GAGAGACACG GGAGGCATAG TGTACTAGTT AGGAATTGCT ATGCAGTTTG
     1081 TCCTGAACGT GTTCACATGA GGTTTCCTAA CATGAGGGCA CTGTCACTAA AGGGTAAGCC
     1141 CCATTTTGCA GAATTCAACC TTGTACCCGC CGGCTGGGGA GCTACCGCCA ACCCGTGGGT
     1201 TGATGCCTGT GCTAGAGCTT GTCCAGGACT GGAGGAACTG AGGTTGAAAT TTATGGTAGT
     1261 GACTGACGAG TGCTTAAAAT TATTATCCCT TTCCTTCACG AATTTTAAGA GCTTGGTTCT
     1321 AGTATGTTGT GAGGGGTTTT CCACGACTGG TTTAGCAAAT ATAGCTACAA ACTGTAGATT
     1381 CCTTAAGGAG CTAGATCTGC AGAAATCTTG TGTCAAGCAC CAAGATCACC AGTGGATTAA
     1441 CTGCTTTCCT AAATCATCTA CATCCCTAGA GTGCCTAAAT TTCAGTTGCC TTACCGGAGA
     1501 GGTGAATGCC GTGGCGCTAG AGGAATTGGT CGCGCGTTCA CCAAACTTAA AATCCTTGAG
     1561 ACTAAATCTG GCTGTCCCGT TCGACGTCCT AAGCCGTATA TTGTCTCGTA CGCCCAAACT
     1621 GGAAGATTTA GGTACTGGTT CTTTTTTGCA GGGAAATGAC CCAGCAGCCT ATGCATCCCT
     1681 GTGCCGTGCG CTTGAAAACT GTACTAGCCT TAAAAGCATT TCAGGGTTTT GGGACGCTCC
     1741 AGGGTTCTAC GTGCAAGGGA TACTAAGTAA TTGCAAAATC AGGAATCTAA CCTGCCTTAA
     1801 TTTGAGCTAT GCAACTTTAA TCCAATCAAC ACAACTAATA GGAATCATAA GACACTGCAA
     1861 GAAACTACAC GTCTTGTGGG TGCTAGACCA TATAGGTGAT GAGGGTCTGA AGGCCGTGAG
     1921 CTTCTCTTGT CCTGATCTGC AGGAGCTTCG TGTCTACCCG TCTGTGGTCG CACCCAGAGG
     1981 GACAGTAACC GAAGAGGGGC TAGTGGCATT GAGCTCCTGT CGTAAGCTAC AACATGTGTT
     2041 GTTTTCCTGC GTTCGTATGA CTAACACGGC CCTAATGACA ATCGCAAGAT ACTGTCCCAG
     2101 GCTAACTTCT TTCCGTCTGT GTATTAGAAA ACCAAGAAGT GCGGACGCGG TAACAGGTCA
     2161 GCCCCTTGAC GAGGGATTCG GTGCAATTGT CCGTTCTTGC AGGGGGTTGA GAAGATTGGC
     2221 GATGAGCGGC CTACTAACAG ATTCCGTTTT CCTTTATATT GGTATGTACG CTGAAAAATT
     2281 GGAGATGTTG AGCGTCACTT TCGCAGGTGA CACCGATGAT GGAATGGTGT ATGTCTTAAA
     2341 CGGATGCAGA AACCTTAAGA AGCTAGTCAT TAAGGAATCA CCATTCGGCG ACGCCGCGTT
     2401 ACTGGCTGGA GCCCACAGAT ACGAGAGCAT GAGGAGTCTG TGGATGTCCT CTTGTCAAAT
     2461 AACTTTGGGG GGTTGCAAGG CTTTAGCCGC CACGATGCCC AACATTAATG TTGAAGTGAT
     2521 AGGTGGAGCC TCTTTTGGAG CTATGGATGG AGGAGTAAGT GGAGCTCGTA AAGTTGATAT
     2581 GTTATATTTG TATAGAACTT TGGCGGGGCC TAGATGCGAT ACCCCTGGGT TCGTGAGTAT
     2641 TCTAGGATct ggtggagatt acaaggatca tgatggtgat tacaaggatc acgacatcga
     2701 ctacaaggat gacgatgaca agcaccatca tcaccaccat tgattctatg tcaggctccc
     2761 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat
     2821 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
     2881 gtttctcgtt cagctttctt gtacaaagtg gtgatgggct gcaggaattc gatatcaagc
     2941 ttatcgatac cgtcgacctc gagtcatgta attagttatg tcacgcttac attcacgccc
     3001 tccccccaca tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc
     3061 tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct
     3121 tttttttctg tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa
     3181 ggttttggga cgctcgaagg ctttaatttg tgacaccgat tatttaaagc tgcagcatac
     3241 gatatatata catgtgtata tatgtatacc tatgaatgtc agtaagtatg tatacgaaca
     3301 gtatgatact gaagatgaca aggtaatgca tcattctata cgtgtcattc tgaacgaggc
     3361 gcgctttcct tttttctttt tgctttttct ttttttttct cttgaactcg agaaaaaaaa
     3421 tataaaagag atggaggaac gggaaaaagt tagttgtggt gataggtggc aagtggtatt
     3481 ccgtaagaac aacaagaaaa gcatttcata ttatggctga actgagcgaa caagtgcaaa
     3541 atttaagcat caacgacaac aacgagaatg gttatgttcc tcctcactta agaggaaaac
     3601 caagaagtgc cagaaataac agtagcaact acaataacaa caacggcggc tacaacggtg
     3661 gccgtggcgg tggcagcttc tttagcaaca accgtcgtgg tggttacggc aacggtggtt
     3721 tcttcggtgg aaacaacggt ggcagcagat ctaacggccg ttctggtggt agatggatcg
     3781 atggcaaaca tgtcccagct ccaagaaacg aaaaggccga gatcgccata tttggtgtcc
     3841 ccgaggatcc aaatttccaa tcttctggta ttaacttcga taactacgat gatattccag
     3901 tggacgcctc tggtaaggat gttcctgaac caatcacaga atttacctca cctccattgg
     3961 acggattgtt attggaaaac atcaaattgg cccgtttcac caagccaaca cctgtgcaaa
     4021 aatactccgt ccctatcgtt gccaacggca gagatttgat ggcctgtgcg cagaccggtt
     4081 ctggtaagac tggtgggttt ttattcccag tgttgtccga atcatttaag actggaccat
     4141 ctcctcaacc agagtctcaa ggctcctttt accaaagaaa ggcctaccca actgctgtca
     4201 ttatggctcc agtttaaacc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4261 acaattccac acaacatagg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     4321 gtgaggtaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4381 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4441 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4501 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4561 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4621 gcgtttttcc ataggctcgg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4681 aggtggcgaa acccgacagg actataaaga taccaggcgt tcccccctgg aagctccctc
     4741 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4801 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4861 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4921 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4981 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5041 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     5101 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5161 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     5221 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5281 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     5341 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5401 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactgccc
     5461 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5521 ccgcgagatc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5581 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5641 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5701 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5761 cgatcaaggc gagttacatg atcccccatg ttgtgaaaaa aagcggttag ctccttcggt
     5821 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5881 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5941 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6001 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6061 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     6121 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6181 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6241 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6301 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6361 cgaaaagtgc cacctgacgt cttattatca tgacattaac ctataaaaat aggcgtatca
     6421 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     6481 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     6541 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     6601 ttgtactgag agtgcaccat aattcgttta aaccgtttta agagcttggt gagcgctagg
     6661 agtcactgcc aggtatcgtt tgaacacggc attagtcagg gaagtcataa cacagtcctt
     6721 tcccgcaatt ttctttttct attactcttg gcctcctcta gtacactcta tattttttta
     6781 tgcctcggta atgattttca tttttttttt tccacctagc ggatgactct ttttttttct
     6841 tagcgattgg cattatcaca taatgaatta tacattatat aaagtaatgt gatttcttcg
     6901 aagaatatac taaaaaatga gcaggcaaga taaacgaagg caaagatgac agagcagaaa
     6961 gccctagtaa agcgtattac aaatgaaacc aagattcaga ttgcgatctc tttaaagggt
     7021 ggtcccctag cgatagagca ctcgatcttc ccagaaaaag aggcagaagc agtagcagaa
     7081 caggccacac aatcgcaagt gattaacgtc cacacaggta tagggtttct ggaccatatg
     7141 atacatgctc tggccaagca ttccggctgg tcgctaatcg ttgagtgcat tggtgactta
     7201 cacatagacg accatcacac cactgaagac tgcgggattg ctctcggtca agcttttaaa
     7261 gaggccctag gggccgtgcg tggagtaaaa aggtttggat caggatttgc gcctttggat
     7321 gaggcacttt ccagagcggt ggtagatctt tcgaacaggc cgtacgcagt tgtcgaactt
     7381 ggtttgcaaa gggagaaagt aggagatctc tcttgcgaga tgatcccgca ttttcttgaa
     7441 agctttgcag aggctagcag aattaccctc cacgttgatt gtctgcgagg caagaatgat
     7501 catcaccgta gtgagagtgc gttcaaggct cttgcggttg ccataagag
//