LOCUS       pML64_pDONR-ZmIAA2      3264 bp ds-DNA     circular     24-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      complement(268..295)
                     /label="rrnB_T2_terminator"
     terminator      complement(427..470)
                     /label="rrnB_T1_terminator"
     misc_feature    522..544
                     /label="M13_pUC_fwd_primer"
     misc_feature    537..553
                     /label="M13_forward20_primer"
     misc_feature    569..668
                     /label="L1"
     misc_recomb     645..665
                     /label="attB1"
     misc_feature    657..673
                     /label="attB1_adaptor_tail"
     misc_feature    669..670
                     /label="TC In Frame"
     misc_feature    671..673
                     /label="Yeast Kozak"
     primer          674..694
                     /label="ZmIAA2_fwd"
                     /note="sequence: AAAAAGCAGGCTTCAAAATGGCTGGAGCCGACGTCGAC"
     misc_feature    674..1384
                     /label="ZmIAA2"
     CDS             674..1384
                     /label="Translation 674-1384"
     primer          complement(1356..1384)
                     /label="ZmIAA2_rev"
                     /note="sequence: AGAAAGCTGGGTGTCAGCTCCTGTTCTTGCACTTGTCCTTCGTTC"
     misc_feature    1385..1387
                     /label="IAA16"
     CDS             1385..1387
                     /label="Translation 1385-1387"
     misc_feature    complement(1385..1400)
                     /label="attB2_tail+STOP"
     misc_feature    1388..1487
                     /label="L2"
     misc_recomb     complement(1392..1412)
                     /label="attB2"
     primer_bind     complement(1505..1526)
                     /label="T7"
     promoter        complement(1506..1524)
                     /label="T7_promoter"
     misc_feature    complement(1525..1543)
                     /label="M13_reverse_primer"
     gene            1658..2467
                     /label="KanR2"
     rep_origin      2598..3217
                     /label="pBR322_origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTC AAAATGGCTG GAGCCGACGT CGACGTCGGG ACGGAGCTCC GCCTCGGCCT
      721 GCCAGGAGGA GGCGCCGATG CGGCCAAGGC TGCCAAGAGG GGCTTCGAGG ACACCATCGA
      781 CCTCAAGCTG AAGCTGCCCA CCGCCGGCAT GGAGGAAGCT GCCGCTGCCG CCGCCGCCAA
      841 GCCGGAGCCG GCCGCCGAGA AGGCCAAGAG GCCCGCCGAG GCGCCGGCCG CTGATGCCGA
      901 GAAGCCACCC GCACCCAAGG CACAAGCTGT GGGTTGGCCA CCAGTCCGGT CATACCGCAG
      961 GAACGTCATG ACCGTCCAGT CTGTGAAGAG CAAGAAGGAA GAGGAGCCTG AGAAGCAGCA
     1021 GTCCGCTGCC AACGCCGGCG GCAACGGCTC CGCCTTCGTG AAGGTCAGCA TGGACGGCGC
     1081 GCCCTACCTG CGCAAGGTGG ACCTGAAGAT GTACAACAGC TACACGGAGC TCTCCGTTGC
     1141 TCTGAAGAAG ATGTTCAGCA CCTTCACCAC ATCCGGGAAC AACATGAATG AGGGAAAACT
     1201 AGTTGATCCG GTCAGCGGTG CTGATGTGGT CACTACTTAC GAAGACAAGG ATGGCGACTG
     1261 GATGCTCGTT GGCGACGTCC CGTGGGAGAT GTTTGTCGAG TCATGCAGGC GTCTGAGGAT
     1321 CATGAAGAGC TCTGAAGCCA TAGGTCTTGC ACCAAGAACG AAGGACAAGT GCAAGAACAG
     1381 GAGCTGACAC CCAGCTTTCT TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT
     1441 TTGTTGCAAC GAACAGGTCA CTATCAGTCA AAATAAAATC ATTATTTGCC ATCCAGCTGA
     1501 TATCCCCTAT AGTGAGTCGT ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT
     1561 GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAATA ATATCATCAT GAACAATAAA
     1621 ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC
     1681 GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG
     1741 CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC
     1801 AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT
     1861 CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC
     1921 TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT
     1981 AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG
     2041 GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC
     2101 TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG
     2161 TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC
     2221 GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA
     2281 ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC
     2341 CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA
     2401 ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT
     2461 TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC TGACTTGACG
     2521 GGACGGCGCA AGCTCATGAC CAAAATCCCT TAACGTGAGT TACGCGTCGT TCCACTGAGC
     2581 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT
     2641 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA
     2701 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT
     2761 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA
     2821 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC
     2881 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG
     2941 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG
     3001 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG
     3061 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT
     3121 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC
     3181 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
     3241 TTGCTGGCCT TTTGCTCACA TGTT
//