LOCUS       pML65_pDONR-ZmIAA5      3213 bp ds-DNA     circular     17-JUN-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      799..1418
                     /label="pBR322_origin"
     terminator      complement(1733..1760)
                     /label="rrnB_T2_terminator"
     terminator      complement(1892..1935)
                     /label="rrnB_T1_terminator"
     misc_feature    1987..2009
                     /label="M13_pUC_fwd_primer"
     misc_feature    2002..2018
                     /label="M13_forward20_primer"
     misc_feature    2034..2133
                     /label="L1"
     misc_recomb     2110..2130
                     /label="attB1"
     misc_feature    2122..2138
                     /label="attB1_adaptor_tail"
     misc_feature    2134..2135
                     /label="TC In Frame"
     misc_feature    2136..2138
                     /label="Yeast Kozak"
     primer          2139..2159
                     /label="ZmIAA5_fwd"
                     /note="sequence: AAAAAGCAGGCTTCAAAATGTCGGTGGAGACGGAGCGG"
     misc_feature    2139..2798
                     /label="ZmIAA5"
     CDS             2139..2798
                     /label="Translation 2139-2798"
     primer          complement(2781..2798)
                     /label="ZmIAA5_rev"
                     /note="sequence: AGAAAGCTGGGTGTCATCGGGCGGCTCTTGGTGC"
     misc_feature    2799..2801
                     /label="IAA16"
     CDS             2799..2801
                     /label="Translation 2799-2801"
     misc_feature    complement(2799..2814)
                     /label="attB2_tail+STOP"
     misc_feature    2802..2901
                     /label="L2"
     misc_recomb     complement(2806..2826)
                     /label="attB2"
     primer_bind     complement(2919..2940)
                     /label="T7"
     promoter        complement(2920..2938)
                     /label="T7_promoter"
     misc_feature    complement(2939..2957)
                     /label="M13_reverse_primer"
     gene            3072..668
                     /label="KanR2"
     CDS             3072..668
                     /label="KanR2"
ORIGIN
        1 CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG
       61 TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA
      121 CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT
      181 TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC
      241 GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG
      301 CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC
      361 GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC
      421 CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA
      481 AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG
      541 CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA
      601 AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT
      661 TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC
      721 GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG
      781 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA
      841 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG
      901 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG
      961 TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT
     1021 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA
     1081 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG
     1141 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC
     1201 GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA
     1261 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC
     1321 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT
     1381 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
     1441 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC
     1501 GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG
     1561 AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT
     1621 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC
     1681 GCAACGCAAT TAATACGCGT ACCGCTAGCC AGGAAGAGTT TGTAGAAACG CAAAAAGGCC
     1741 ATCCGTCAGG ATGGCCTTCT GCTTAGTTTG ATGCCTGGCA GTTTATGGCG GGCGTCCTGC
     1801 CCGCCACCCT CCGGGCCGTT GCTTCACAAC GTTCAAATCC GCTCCCGGCG GATTTGTCCT
     1861 ACTCAGGAGA GCGTTCACCG ACAAACAACA GATAAAACGA AAGGCCCAGT CTTCCGACTG
     1921 AGCCTTTCGT TTTATTTGAT GCCTGGCAGT TCCCTACTCT CGCGTTAACG CTAGCATGGA
     1981 TGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTCT TAAGCTCGGG CCCCAAATAA
     2041 TGATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA AATTGATGAG CAATGCTTTT
     2101 TTATAATGCC AACTTTGTAC AAAAAAGCAG GCTTCAAAAT GTCGGTGGAG ACGGAGCGGA
     2161 GCTCCACCGA GTCCTCCGGG GCTTCCGGGC TCGACTTCGA GGACACCGCG CTCACGCTCA
     2221 CCCTCCGCCT CCCGGGCTCC GCGCCTTCCG CCGCCGCCGC CGCCGCGGCT TCCCTGTCCC
     2281 TCTCCTCCTC GTCGTCCTCC GCCTTCCCCG ACCCCGACCG CAAGCGCGCC TCCTCCGACG
     2341 CTGACCCCGG CCGCTCCTCC CCGCTCGCCG CGTCCTCCGA CGCTGCACCG GCACCCAAGG
     2401 CTCGTGTGGT GGGCTGGCCG CCGGTGAGGT CGTACCGCAA GAACGCGCTC GCCGACGCCG
     2461 CGGGCTCCAG CAAGGCCGCC AAGTTCGTCA AGGTGGCCGT CGACGGCGCG CCCTACCTGC
     2521 GGAAGGTGGA CCTGCAGGCG TACGCCGGCT ACGACCAGCT GCTCCGCGCG CTCCAGGACA
     2581 AGTTCTTCTC CCACTTCACC ATCAGGAAGT TCGCCGACGA CGAGAGGAAG CTGGTGGACG
     2641 CGGTGAACGG GACGGAGTAC GTACCCACGT ACGAGGACAA GGATGGCGAC TGGATGCTCG
     2701 TCGGCGACGT CCCCTGGAAG ATGTTCGTGG AAACCTGCCA GCGCCTTCGT CTGATGAAAG
     2761 GTTCAGAGGC CGTGAACTTG GCACCAAGAG CCGCCCGATG ACACCCAGCT TTCTTGTACA
     2821 AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA
     2881 GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC CTATAGTGAG TCGTATTACA
     2941 TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA AAATCTCTGA TGTTACATTG
     3001 CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA
     3061 CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG GCCGCGATTA AATTCCAACA
     3121 TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA
     3181 CAATCTATCG CTTGTATGGG AAGCCCGATG CGC
//